| Budget Amount *help |
¥195,260,000 (Direct Cost: ¥150,200,000、Indirect Cost: ¥45,060,000)
Fiscal Year 2018: ¥33,410,000 (Direct Cost: ¥25,700,000、Indirect Cost: ¥7,710,000)
Fiscal Year 2017: ¥33,410,000 (Direct Cost: ¥25,700,000、Indirect Cost: ¥7,710,000)
Fiscal Year 2016: ¥33,410,000 (Direct Cost: ¥25,700,000、Indirect Cost: ¥7,710,000)
Fiscal Year 2015: ¥44,590,000 (Direct Cost: ¥34,300,000、Indirect Cost: ¥10,290,000)
Fiscal Year 2014: ¥50,440,000 (Direct Cost: ¥38,800,000、Indirect Cost: ¥11,640,000)
|
|---|
| Outline of Final Research Achievements |
This project aimed to reveal biological processes mediated by RNA modifications. We discovered a cap-specific novel m6A writer for mRNA, and an RNA acetyltransferase using an acetate ion as a substrate. We studied CO2-sensitive tRNA modification and its association with Warburg effect, found dynamic regulation of tRNA modification by sensing cellular metabolic status, and reported pre-tRNA capping. We also showed that a single methylation of 23S rRNA plays a critical role in ribosomal 50S subunit assembly in the late stage. We elucidated biogenesis of 5-formylcytidine in human mitochondrial tRNA. These observations contribute to our deep understanding of functional and physiological roles of RNA modifications as epitranscriptome marks.
|
