| Budget Amount *help |
¥195,260,000 (Direct Cost: ¥150,200,000、Indirect Cost: ¥45,060,000)
Fiscal Year 2018: ¥36,140,000 (Direct Cost: ¥27,800,000、Indirect Cost: ¥8,340,000)
Fiscal Year 2017: ¥36,140,000 (Direct Cost: ¥27,800,000、Indirect Cost: ¥8,340,000)
Fiscal Year 2016: ¥36,140,000 (Direct Cost: ¥27,800,000、Indirect Cost: ¥8,340,000)
Fiscal Year 2015: ¥36,140,000 (Direct Cost: ¥27,800,000、Indirect Cost: ¥8,340,000)
Fiscal Year 2014: ¥50,700,000 (Direct Cost: ¥39,000,000、Indirect Cost: ¥11,700,000)
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| Outline of Final Research Achievements |
We have developed a unique platform to systematically identify target molecules of bioactive compounds by utilizing novel technologies such as molecular barcode sequencing and bimolecular luminescence complementation. Specifically, an integrated method to detect chemical genomic interaction using barcoded gene disruptant libraries of both budding and fission yeasts, a barcoded, pooled shRNA viral library, and a split luciferase-based mammalian three-hybrid system was established. By using this method as well as a conventional physical interaction screening using compound-conjugated beads, we could identify target molecules and the mechanisms of action of several target-unknown metabolites from microbes and marine organisms.
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