|Budget Amount *help
¥40,690,000 (Direct Cost: ¥31,300,000、Indirect Cost: ¥9,390,000)
Fiscal Year 2017: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2016: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2014: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
|Outline of Final Research Achievements
DNA double-strand breaks (DSBs) are deleterious DNA damage. DSBs are repaired by two major DNA repair pathways: Non Homologous End Joining and Homologous Recombination (HR). RNF8 has been known as a molecule that promotes NHEJ and suppresses HR. In this research, we revealed that RNF8 promotes HR in BRCA1-negative cells. 2. DSBs are utilized for gene editing technology. However, DSBs can induce gene mutations during gene editing with DSBs. We have developed DSB-free new gene editing methods and revealed molecular mechanisms working in the gene editing technology.