Studies on genome integrity of archaea in the extremophilic environments
Project/Area Number |
26242075
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biomolecular chemistry
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Research Institution | Kyushu University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
内海 利男 新潟大学, 自然科学系, 教授 (50143764)
金井 昭夫 慶應義塾大学, 環境情報学部, 教授 (60260329)
跡見 晴幸 京都大学, 工学(系)研究科(研究院), 教授 (90243047)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000)
Fiscal Year 2016: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2015: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2014: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
|
Keywords | 超好熱性アーキア / ゲノム安定性維持 / 遺伝情報 / DNA修復 / RNAプロセシング / 翻訳装置 / 極限環境 / Thermococcales / 生体機能物質 / アーキア / 遺伝情報伝達系 / 極限環境生命 |
Outline of Final Research Achievements |
We discovered novel repair enzymes from hyperthermophilic archaea and named them EndoQ and EndoMS. EndoQ works for deaminated base repair. EndoMS is expected to be a repair enzyme for mismatched bases. These findings are the breakthrough for understanding of DNA repair systems in Archaea. By constructing an in vitro reconstitution system using PfEndA, PfRtcB, PfArchaease, and precursor tRNA, understanding of the reaction mechanism of tRNA processing has greatly progressed. PF0374 and PF0560 were discovered as novel regulation factors in the translation process. Their ribosome-binding property and each specific function were elucidated. With these discoveries, understanding of the mechanisms for genome integrity under extreme environments has greatly advanced.
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Report
(4 results)
Research Products
(190 results)
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[Journal Article] Exonuclease processivity of archaeal replicative DNA polymerase in association with PCNA is expedited by mismatches in DNA.2017
Author(s)
Yoda, T., Tanabe, M., Tsuji, T., Yoda, T., Ishino, S., Shirai, T., Ishino, Y., Takeyama, H., and Nishida, H.
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Journal Title
Sci. Rep.
Volume: 7
Issue: 1
Pages: 44582-44582
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Atomic structure of an archaeal GAN suggests its dual roles as an exonuclease in DNA repair and a CMG component in DNA replication.2016
Author(s)
Oyama, T., Ishino, S., Shirai, T., Yamagami, T., Nagata, M., Ogino, H., Kusunoki, M., and Ishino, Y.
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Journal Title
Nucleic Acids, Res.
Volume: 44
Issue: 19
Pages: 9509-9517
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] PCNA is involved in the EndoQ-mediated DNA repair process in Thermococcales.2016
Author(s)
Shiraishi, M., Ishino, S., Yoshida, K., Yamagami, T., Cann, I., and Ishino, Y.
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Journal Title
Sci. Rep.
Volume: 6
Issue: 1
Pages: 25532-25532
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] A longer finger-subdomain of family A DNA polymerases found by metagenomic analysis strengthens DNA binding and primer extension abilities.2016
Author(s)
Yamagami, T., Matsukawa, H., Tsunekawa, S., Kawarabayasi, Y., Ishino, S., and Ishino, Y
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Journal Title
Gene
Volume: 576
Issue: 2
Pages: 690-695
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Mutation of the gene encoding the ribonuclease P RNA in the hyperthermophilic archaeon Thermococcus kodakarensis causes decreased growth rate and impaired processing of tRNA precursors.2015
Author(s)
Ueda, T., Ishino, S., Suematsu K., Nakashima, T., Kakuta, Y., Kawarabayasi, Y., Ishino, Y., and Kimura, M.
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Journal Title
Biochem Biophys Res Commun.
Volume: 468
Issue: 4
Pages: 660-665
DOI
Related Report
Peer Reviewed
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[Journal Article] Guanine nucleotide exchange factor 2 for Rab5 proteins coordinated with GLUP6/GEF regulates the intracellular transport of the proglutelin from the Golgi apparatus to the protein storage vacuole in rice endosperm2015
Author(s)
Wen, L., Fukuda, M., Sunada, M., Ishino, S., Ishino, Y., Okita, W.T., Ogawa, M., Ueda, T. and Kumamaru, T.
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Journal Title
J. Exp. Bot.
Volume: 66
Issue: 20
Pages: 6137-6147
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Functional role of the C-terminal tail of the archaeal ribosomal stalk in recruitment of two elongation factors to the sarcin/ricin loop of 23S rRNA.2015
Author(s)
Imai, H, Miyoshi, T, Murakami, R, Ito, K, Ishino, Y, and Uchiumi, T.
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Journal Title
Genes. Cells
Volume: 20
Issue: 7
Pages: 613-624
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] An optimized Npro-based method for expression and purification of intrinsically disordered proteins for NMR study.2015
Author(s)
Goda, N., Matsuo, N., Tenno, T., Ishino, S., Ishino, Y., Fukuchi, S., Ota, M., and Hiroaki, H.
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Journal Title
J. Intrinsic. Disordered Prot.
Volume: 3
Pages: 1-6
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Characterization of anti-P monoclonal antibodies directed against the ribosomal protein•RNA complex antigen and produced using MRL autoimmune-prone mice.2015
Author(s)
Sato, H., Onozuka, M., Hagiya, A., Hoshino, S., Narita, I., and Uchiumi T.
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Journal Title
Clin. Exp. Immunol.
Volume: 179
Issue: 2
Pages: 236-244
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] A novel endonuclease that may be responsible for damaged DNA base repair in Pyrococcus furiosus2015
Author(s)
Shiraishi, M., Ishino, S., Yamagami, T., Egashira, Y., Kiyonari, S., and Ishino, Y.
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Journal Title
Nucleic Acids Res
Volume: 5
Issue: 5
Pages: 2853-2863
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Activation of the MCM helicase from the thermophilic archaeon, Thermoplasma acidophilum by interactions with GINS and Cdc6-2.2014
Author(s)
Ogino, H., Ishino, S., Haugland, G.T., Birkeland, N-K., Kohda, D., and Ishino, Y.
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Journal Title
Extremophiles
Volume: 18
Issue: 5
Pages: 915-924
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Multiple Interactions of the Intrinsically Disordered Region between the N-terminal Helicase and C-terminal Nuclease Domains2014
Author(s)
S. Ishino, T. Yamagami, M. Kitamura, N. Kodera, T. Mori, S. Sugiyama, T. Ando, N. Goda, T. Tenno, H. Hiroaki, and Y. Ishino
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Journal Title
J. Biol. Chem.
Volume: 289
Issue: 31
Pages: 21627-21639
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular insights into the interaction of the ribosomal stalk protein with elongation factor 1α.2014
Author(s)
Ito, K., Honda, T., Suzuki, T., Miyoshi, T., Murakami, R., Yao, M., and Uchiumi T.
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Journal Title
Nucleic Acids Res.
Volume: 42
Issue: 22
Pages: 14042-14052
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Crystal structure of α-amylase from Oryza sativa : Molecular insights into enzyme activity and thermostability2014
Author(s)
Akihito OCHIAI, Hiroshi SUGAI, Kazuki HARADA, Seiya TANAKA, Yohei ISHIYAMA, Kosuke ITO, Takaaki TANAKA, Toshio UCHIUMI, Masayuki TANIGUCHI, Toshiaki MITSUI
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Journal Title
Bioscience, Biotechnology, and Biochemistry
Volume: (In press)
Issue: 6
Pages: 989-997
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Structure and functions of the Thermococcal-specific exonuclease.2015
Author(s)
Sonoko Ishino, Ken-ichi Miyazono, Takeshi Yamagami, Takafumi Hamasuna, Tsuyoshi Shirai, Hiroya Tomita, Tamotsu Kanai, Haruyuki Atomi, Masaru Tanokura, Yoshizumi Ishino.
Organizer
2015 International Chemical Congress of Pacific Basin Societies.
Place of Presentation
Honolulu, Hawaii, USA
Year and Date
2015-12-15
Related Report
Int'l Joint Research / Invited
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[Presentation] Structure and functions of the replicative helicase complex in Archaea.2015
Author(s)
Sonoko Ishino, Hiromi Ogino, Mariko Nagata, Kohei Kobayashi, Takuji Oyama, Takeshi Yamagami, Jan-Robert Simmons, Tamotsu Kanai, Haruyuki Atomi, Yutaka Kawarabayasi, Yoshizumi Ishino.
Organizer
第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
Place of Presentation
神戸ポートアイランド、神戸
Year and Date
2015-12-01
Related Report
Invited
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