| Budget Amount *help |
¥44,330,000 (Direct Cost: ¥34,100,000、Indirect Cost: ¥10,230,000)
Fiscal Year 2016: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2015: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2014: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
|
|---|
| Outline of Final Research Achievements |
We discovered novel repair enzymes from hyperthermophilic archaea and named them EndoQ and EndoMS. EndoQ works for deaminated base repair. EndoMS is expected to be a repair enzyme for mismatched bases. These findings are the breakthrough for understanding of DNA repair systems in Archaea. By constructing an in vitro reconstitution system using PfEndA, PfRtcB, PfArchaease, and precursor tRNA, understanding of the reaction mechanism of tRNA processing has greatly progressed. PF0374 and PF0560 were discovered as novel regulation factors in the translation process. Their ribosome-binding property and each specific function were elucidated. With these discoveries, understanding of the mechanisms for genome integrity under extreme environments has greatly advanced.
|
