in vivo super-resolution imaging by utilizing novel laser technologies
| Project/Area Number |
26242082
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic / Social brain science
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| Research Institution | Hokkaido University |
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Principal Investigator |
Nemoto Tomomi 北海道大学, 電子科学研究所, 教授 (50291084)
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 俊一 東北大学, 多元物質科学研究所, 教授 (30162431)
川上 良介 北海道大学, 電子科学研究所, 助教 (40508818)
大友 康平 北海道大学, 電子科学研究所, 特任助教 (40547204)
日比 輝正 北海道大学, 電子科学研究所, 特任講師 (50554292)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥44,460,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥10,260,000)
Fiscal Year 2016: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2015: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2014: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
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| Keywords | ２光子顕微鏡 / 超短光パルスレーザー / ベクトルビーム / バイオイメージング / 神経科学 / スパイン / 超解像 / 光操作 / インビボ・イメージング / 光脳科学 / 超解像顕微鏡 / 応用光学 / イメージング / 光渦 / in vivoイメージング / 光ファイバー増幅器 / 多光子顕微鏡 |
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| Outline of Final Research Achievements |
We successfully generated vector beams, optical vortex by using liquid crystal devices and developed a super-resolution microscope, two-photon STED microscope. In addition, we developed an excitation wavelength unmixing method useful for multicolor staining specimens. For in vivo imaging of living mouse brains, adaptive optics improved spatial resolutions and enabled laser ablation of neural fibers. In the case of depression model mice, a super-resolution microscopy could demonstrate quite finer morphological changes of dendritic spines that had not detected by conventional confocal microscopy. Moreover, numerical analyses on super-resolution effects showed that microscopy using vector beams can observe finer structure in specimens clearly, suggesting a superiority of vector beams.
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Report
(4 results)
Research Products
(162 results)
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[Presentation] Transmissive liquid-crystal device correcting primary coma aberration and astigmatism in laser scanning microscopy2016
Author(s)
Ayano Tanabe, Terumasa Hibi, Sari Ipponjima, Kenji Matsumoto, Masafumi Yokoyama, Makoto Kurihara, Nobuyuki Hashimoto, Tomomi Nemoto
Organizer
BiOS, SPIE
Place of Presentation
The Moscone Center, San Francisco, CA (USA)
Year and Date
2016-02-07
Related Report
Int'l Joint Research
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[Presentation] 透過型液晶収差補正素子の開発と生体組織標本への応用2015
Author(s)
田辺綾乃,日比輝正,一本嶋佐理,松本健志,横山正史,栗原誠,橋本信幸,根本知己
Organizer
日本光学会年次学術講演会Optics & Photonics Japan 2015
Place of Presentation
筑波大学東京キャンパス文京校舎（東京都・文京区）
Year and Date
2015-10-28
Related Report
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[Presentation] Transmissive liquid crystal devices correcting the spherical aberrations in laser scanning microscopy2015
Author(s)
Ayano Tanabe, Terumasa Hibi, Kenji Matsumoto, Masafumi Yokoyama, Makoto Kurihara, Sari Ipponjima, Nobuyuki Hashimoto, Tomomi Nemoto,
Organizer
BiOS, SPIE
Place of Presentation
The Moscone Center (San Francisco, USA)
Year and Date
2015-02-07
Related Report
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[Presentation] 膵外分泌・内分泌における膜融合関連分子SNAP23の機能の解明2014
Author(s)
國井政孝, 吉村信一郎, 高橋倫子, 小林 雅樹, 佐藤隆史, 川上良介, 根本知己, 河西晴郎, 北村忠弘, 佐藤 健,原田彰宏
Organizer
第66回日本細胞生物学会大会
Place of Presentation
奈良県新公会堂東大寺総合文化センター（奈良県・奈良市）
Year and Date
2014-06-13
Related Report
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