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Development of norovirus detection system utilizing time-resolved fluorescence imaging excited by surface plasmon resonance

Research Project

Project/Area Number 26246008
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Nanobioscience
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

FUJIMAKI Makoto  国立研究開発法人産業技術総合研究所, 電子光技術研究部門, 研究グループ長 (10392656)

Co-Investigator(Kenkyū-buntansha) 香川 景一郎  静岡大学, 電子工学研究所, 准教授 (30335484)
谷口 孝喜  藤田保健衛生大学, 医学部, 教授 (40094213)
染谷 雄一  国立感染症研究所, ウイルス第二部, 主任研究官 (50283809)
守口 匡子  藤田保健衛生大学, 医学部, 講師 (60298528)
白土 東子 (堀越東子 / 白土 東子(堀越東子))  国立感染症研究所, ウイルス第二部, 主任研究官 (60356243)
久保田 智巳  国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (90356923)
芦葉 裕樹  国立研究開発法人産業技術総合研究所, 電子光技術研究部門, 研究員 (90712216)
Co-Investigator(Renkei-kenkyūsha) KAWAHITO Shoji  静岡大学, 電子工学研究所, 教授 (40204763)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2016: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
Fiscal Year 2015: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
Fiscal Year 2014: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Keywordsバイオセンサ / 表面プラズモン共鳴 / 蛍光増強 / CMOSイメージセンサ / 時間分解測定 / 交差反応性ノロウイルスファージ抗体 / 抗体の結晶化 / バイオチップ / 交差反応性抗ノロウイルスファージ抗体
Outline of Final Research Achievements

A property analysis of 12A11 antibody, which is an anti-norovirus antibody that we developed, was performed, and it was confirmed that the antibody reacted to several different genotype noroviruses. We showed that it was possible to detect approximately 100 virus-like particles of norovirus existing in the detection area of a fluorescent-detection-type biosensor utilizing a surface plasmon resonance excitation layer of aluminum, in which the antibody and a quantum dot fluorescent dye were used. It was also confirmed that a time-resolved fluorescence imaging method can successfully distinguish noises emitted from sensor chip substrates from signals of the fluorescent dye. The sensitivity of the sensor was improved quadruple by introducing the time-resolved fluorescence imaging.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (34 results)

All 2017 2016 2015 2014 Other

All Journal Article (7 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 4 results) Presentation (19 results) (of which Int'l Joint Research: 8 results,  Invited: 4 results) Book (2 results) Remarks (1 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 1 results)

  • [Journal Article] Detection of norovirus virus-like particles using a surface plasmon resonance-assisted fluoroimmunosensor optimized for quantum dot fluorescent labels2017

    • Author(s)
      H. Ashiba, Y. Sugiyama, X. Wang, H. Shirato, K. Moriguchi, K. Taniguchi, Y. Ohki, M. Fujimaki
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 93 Pages: 260-266

    • DOI

      10.1016/j.bios.2016.08.099

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A programmable sub-nanosecond time-gated 4-tap lock-in pixel CMOS image sensor for real-time fluorescence lifetime imaging microscopy2017

    • Author(s)
      M. -W. Seo, Y. Shirakawa, Y. Masuda, Y. Kawata, K. Kagawa, K. Yasutomi, S. Kawahito
    • Journal Title

      ISSCC Dig. Tech. Papers

      Volume: - Pages: 70-71

    • DOI

      10.1109/isscc.2017.7870265

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A high performance multi-tap CMOS lock-in pixel image sensor for biomedical applications2017

    • Author(s)
      M. -W. Seo, Y. Shirakawa, K. Kagawa, K. Yasutomi, S. Kawahito
    • Journal Title

      Photonics West 2017, Proc. SPIE

      Volume: 10076 Pages: 100760V-100760V

    • DOI

      10.1117/12.2255951

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Design of an 8-tap CMOS lock-in pixel with lateral electric field charge modulator for highly time-resolved imaging2017

    • Author(s)
      Y. Shirakawa, M-W. Seo, K. Yasutomi, K. Kagawa, N. Teranishi, S. Kawahito
    • Journal Title

      Photonics West 2017, Proc. SPIE

      Volume: 10108 Pages: 101080N-101080N

    • DOI

      10.1117/12.2251788

    • NAID

      40021162425

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Detection of extremely low concentrations of biological substances using near-field illumination2016

    • Author(s)
      M. Yasuura, M. Fujimaki
    • Journal Title

      Scientific Reports

      Volume: 6:39241 Issue: 1 Pages: 1-7

    • DOI

      10.1038/srep39241

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A CMOS image sensor with lateral electric field modulation pixels for sub nano-second time response fluorescence lifetime imaging2016

    • Author(s)
      Zhuo Li, Min-Woong Seo, Keiichiro Kagawa, Keita Yasutomi, Shoji Kawahito
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: Vol. 55 Issue: 4S Pages: 04EM06-04EM06

    • DOI

      10.7567/jjap.55.04em06

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A 10 ps Time-Resolution CMOS Image Sensor With Two-Tap True-CDS Lock-In Pixels for Fluorescence Lifetime Imaging2016

    • Author(s)
      M-W. Seo, K. Kagawa, K. Yasutomi, N. Teranishi, Z. Li, I.A. Halin, S. Kawahito
    • Journal Title

      IEEE Journal of Solid-State Circuits

      Volume: Vol.51. No. 1 Issue: 1 Pages: 141-154

    • DOI

      10.1109/jssc.2015.2496788

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] An external force-assisted near-field illumination biosensor for detection of extremely low concentrations of biological substances2017

    • Author(s)
      M. Fujimaki
    • Organizer
      The 24th Congress of the International Commission for Optics
    • Place of Presentation
      京王プラザホテル(東京)
    • Year and Date
      2017-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 極低濃度のバイオ物質を検出するには2017

    • Author(s)
      藤巻 真
    • Organizer
      Joint International Workshop of WFF&WFSM
    • Place of Presentation
      北海道大学(北海道)
    • Year and Date
      2017-03-02
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] A programmable sub-nanosecond time-gated 4-tap lock-in pixel CMOS image sensor for real-time fluorescence lifetime imaging microscopy2017

    • Author(s)
      M. -W. Seo, Y. Shirakawa, Y. Masuda, Y. Kawata, K. Kagawa, K. Yasutomi, S. Kawahito
    • Organizer
      2017 IEEE International Solid- State Circuits Conference
    • Place of Presentation
      San Francisco Marriott, San Francisco (USA)
    • Year and Date
      2017-02-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A high performance multi-tap CMOS lock-in pixel image sensor for biomedical applications2017

    • Author(s)
      M. -W. Seo, Y. Shirakawa, K. Kagawa, K. Yasutomi, S. Kawahito
    • Organizer
      Photonics West 2017
    • Place of Presentation
      Moscone Center, San Francisco (USA)
    • Year and Date
      2017-01-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Design of an 8-tap CMOS lock-in pixel with lateral electric field charge modulator for highly time-resolved imaging2017

    • Author(s)
      Y. Shirakawa, M-W. Seo, K. Yasutomi, K. Kagawa, N. Teranishi, S. Kawahito
    • Organizer
      Photonics West 2017
    • Place of Presentation
      Moscone Center, San Francisco (USA)
    • Year and Date
      2017-01-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A Lateral Electric Field Charge Modulator with Bipolar-gates for High Time-Resolved Imaging2016

    • Author(s)
      Y. Morikawa, K. Yasutomi, S. Imanishi, T. Takasawa, K. Kagawa, N. Teranishi, S. Kawahito
    • Organizer
      3rd International Workshop on Image Sensors and Imaging Systems
    • Place of Presentation
      東京工業大学(東京都)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Aluminum-based V-trench biosensor for plasmon-assisted fluoroimmunoassay of norovirus virus-like particles2016

    • Author(s)
      H. Ashiba, Y. Sugiyama, Y. Ohki, X. Wang, M. Fujimaki
    • Organizer
      Biosensors 2016
    • Place of Presentation
      Gothenburg, (Sweden)
    • Year and Date
      2016-05-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multi-aperture ultra-high-speed imaging with lateral electric field charge modulator2016

    • Author(s)
      K. Kagawa, F. Mochizuki, M. -W, Seo, K. Yasutomi, S. Kawahito
    • Organizer
      Photonics West 2016
    • Place of Presentation
      San Francisco, (USA)
    • Year and Date
      2016-02-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] V溝バイオセンサーによる免疫アッセイ2016

    • Author(s)
      藤巻 真
    • Organizer
      生物化学的測定研究会 第21回 学術シンポジウム
    • Place of Presentation
      産業技術総合研究所関西センター(大阪府)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Computational modeling of carbohydrate recognition in norovirus capsid protein with lewis antigen2015

    • Author(s)
      T. Ishida, T. Kubota, H. Shirato
    • Organizer
      THE INTERNATIONAL CHEMICAL CONGRESS OF PACIFIC BASIN SOCIETIES 2015
    • Place of Presentation
      Honolulu, (USA)
    • Year and Date
      2015-12-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ノロウイルスのウイルス様粒子のV溝バイオセンサーによる検出2015

    • Author(s)
      杉山勇輝、黒田千愛、大木義路、芦葉裕樹、王暁民、藤巻真
    • Organizer
      2015年度 放電学会 年次大会
    • Place of Presentation
      防衛大学校(神奈川県横須賀市)
    • Year and Date
      2015-12-05
    • Related Report
      2015 Annual Research Report
  • [Presentation] ノロウィルスキャプシドタンパク質の糖鎖認識における糖鎖構造依存性2015

    • Author(s)
      石田豊和、久保田智巳、白土東子
    • Organizer
      第9回分子科学討論会
    • Place of Presentation
      東京工業大学 大岡山キャンパス(東京都)
    • Year and Date
      2015-09-16
    • Related Report
      2015 Annual Research Report
  • [Presentation] V溝バイオセンサーへのカーボンナノチューブ蛍光標識の適用2015

    • Author(s)
      芦葉裕樹、藤巻真、王暁民、飯泉陽子、湯田坂雅子、岡崎俊也
    • Organizer
      第76回応用物理学会秋季学術講演会
    • Place of Presentation
      名古屋国際会議(愛知県)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] V溝バイオセンサーを用いたノロウイルス様粒子の検出2015

    • Author(s)
      杉山勇輝、黒田千愛、大木義路、芦葉裕樹、王暁民、藤巻真
    • Organizer
      第76回応用物理学会秋季学術講演会
    • Place of Presentation
      名古屋国際会議(愛知県)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] ノロウィルスキャプシドタンパク質と血液型糖鎖抗原との相互作用の結晶学的解析2015

    • Author(s)
      久保田智巳、伊藤浩美、成松久、石田豊和、白土東子
    • Organizer
      第15回日本蛋白質科学会年会
    • Place of Presentation
      あわぎんホール(徳島県)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] ノロウィルスキャプシドタンパク質とルイス抗原との糖鎖認識:計算科学による相互作用解析2015

    • Author(s)
      石田豊和、久保田智巳、白土東子
    • Organizer
      第15回日本蛋白質科学会年会
    • Place of Presentation
      あわぎんホール(徳島県)
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] ノロウィルスキャプシドタンパク質と血液型糖鎖抗原との糖鎖認識機構2015

    • Author(s)
      石田豊和、久保田智巳、白土東子
    • Organizer
      第18回理論化学討論会
    • Place of Presentation
      大阪大学会館(大阪府)
    • Year and Date
      2015-05-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 量子ドットを蛍光標識としたV溝バイオセンサーの設計2015

    • Author(s)
      藤巻真、芦葉裕樹、王暁民、粟津浩一
    • Organizer
      第62回応用物理学会春季学術講演会
    • Place of Presentation
      東海大学湘南キャンパス(神奈川県)
    • Year and Date
      2015-03-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] 結晶構造を基にしたノロウイルスキャプシドタンパク質とルイス抗原との結合エネルギー解析2014

    • Author(s)
      久保田智巳、石田豊和、白土東子
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2014-11-26
    • Related Report
      2014 Annual Research Report
  • [Book] 最新医学、第70巻11月増刊号、「ノロウイルスと血液型抗原」2015

    • Author(s)
      白土東子
    • Total Pages
      205
    • Publisher
      最新医学社
    • Related Report
      2015 Annual Research Report
  • [Book] Glycoscience: Biology and Medicine2014

    • Author(s)
      Naoyuki Taniguchi, Haruko Shirato ほか
    • Total Pages
      1556
    • Publisher
      Springer Japan
    • Related Report
      2014 Annual Research Report
  • [Remarks] 産業技術総合研究所 電子光技術研究部門 光センシンググループ

    • URL

      https://unit.aist.go.jp/esprit/opt-sens/index.html

    • Related Report
      2014 Annual Research Report
  • [Patent(Industrial Property Rights)] 光学的検出方法及び光学的検出装置2017

    • Inventor(s)
      安浦雅人、藤巻真、芦葉裕樹、島隆之
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017-02-23
    • Related Report
      2016 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 光学的検出方法及び光学的検出装置2016

    • Inventor(s)
      安浦雅人、藤巻真、芦葉裕樹
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-090662
    • Filing Date
      2016-04-28
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 標的物質検出装置及び標的物質検出方法2016

    • Inventor(s)
      安浦雅人、藤巻真、芦葉裕樹、島隆之
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-232382
    • Filing Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 標的物質検出装置及び標的物質検出方法2016

    • Inventor(s)
      安浦雅人、藤巻真、芦葉裕樹、島隆之
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-232383
    • Filing Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 光学的検出方法及び光学的検出装置2016

    • Inventor(s)
      安浦雅人、藤巻真、芦葉裕樹、島隆之
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-245113
    • Filing Date
      2016-12-19
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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