Contact Irreversible Electroporation of Cells and Tissues under the Control of the Electric Field and Temperature

• Project/Area Number: 26249021
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Thermal engineering
• Research Institution: Kyushu University
• Principal Investigator: Takamatsu Hiroshi 九州大学, 工学研究院, 教授 (20179550)
• Co-Investigator(Kenkyū-buntansha): 藏田 耕作 九州大学, 工学研究院, 准教授 (00368870) ; Wang Haidong (WANG Haidong) 九州大学, 工学研究院, 助教 (30729405) ; 福永 鷹信 九州大学, 工学研究院, 技術職員 (60591196)
• Co-Investigator(Renkei-kenkyūsha): SUMIMOTO Hideki 九州大学, 大学院医学研究院, 教授 (30179303) ; OYA Yusuke 琉球大学, 大学院医学研究科, 教授 (30240964)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥42,900,000 (Direct Cost: ¥33,000,000、Indirect Cost: ¥9,900,000); Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥31,070,000 (Direct Cost: ¥23,900,000、Indirect Cost: ¥7,170,000)
• Keywords: 不可逆エレクトロポレーション / 低侵襲治療 / 温度計測 / デバイス開発 / 微小電極 / 低電圧化 / アジュバント / 低侵襲医療 / 高電圧パルス利用 / 生体医工学 / 温度測定

Outline of Final Research Achievements

This study aimed to develop contact irreversible electroporation for treatment of superficial tumors by using electric pulses at extremely reduced voltages. We first developed a method to measure the temperature rise associated with considerably short electric pulses. We also showed that transient temperature rise during electroporation could be estimated by numerical analysis. Protein denaturation due to the excess electric pulses was detectable by using Raman spectroscopic measurement. We then fabricated comb-shaped miniature contact electrodes with the size and geometry optimized by numerical analysis. The electroporation with the electrode successfully necrotized the cells at the surface of a tissue phantom. Furthermore, the depth of the necrotized tissue was controllable by selecting the pulse parameters. We also confirmed that the use of adjuvants could reduce the magnitude of the electric field required for the treatment by up to 30%.

Research Products

• [Journal Article] Raman microspectroscopic detection of thermal denaturation associated with irreversible electroporation (2017)
  • Author(s): Kosaku Kurata, Takashi Yoshii, Yoshihiro Deguchi, Hiroshi Takamatsu
  • Journal Title: International Journal of Heat and Mass Transfer Volume: 111 Pages: 163-170
  • DOI: 10.1016/j.ijheatmasstransfer.2017.03.115
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Development of contact irreversible electroporation using a comb-shaped miniature electrode (2017)
  • Author(s): Shuto Yoshimatsu, Masahiro Yoshida, Kosaku Kurata, Hiroshi Takamatsu
  • Journal Title: Journal of Thermal Science and Technology Volume: 12 Issue: 2 Pages: JTST0023-JTST0023
  • DOI: 10.1299/jtst.2017jtst0023
  • NAID: 130006022854
  • ISSN: 1880-5566
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Irreversible electroporation: Medical application of intense electric pulses for sustainable health (2016)
  • Author(s): Kosaku Kurata, Hiroshi Takamatsu
  • Journal Title: AIP Conference Proceedings Volume: 1717 Pages: 020002-020002
• [Journal Article] Irreversible electroporation: Medical application of intense electric pulses for sustainable health (2016)
  • Author(s): Kosaku Kurata and Hiroshi Takamatsu
  • Journal Title: AIP Conference Proceedings Volume: - Pages: 020002-020002
  • DOI: 10.1063/1.4943424
  • Acknowledgement Compliant
• [Journal Article] Engineering approach to irreversible electroporation (2014)
  • Author(s): Hiroshi Takamatsu, Kosaku Kurata
  • Journal Title: Proceedings of the 16th International Heat Transfer Conference Volume: IHTC15-KN05 Pages: 18-18
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 微小櫛形電極を利用した生体ファントムの非熱的不可逆エレクトロポレーション (2018)
  • Author(s): 太田冬里，中島彬，藏田耕作，高松洋
  • Organizer: 第30回バイオエンジニアリング講演会
• [Presentation] 接触式櫛形電極を用いた生体模擬組織の低電圧不可逆エレクトロポレーション (2017)
  • Author(s): 吉松秀隼, 藏田耕作, 高松洋
  • Organizer: 第54回日本伝熱シンポジウム
• [Presentation] Effect of surfactant addition on the threshold of irreversible electroporation (2017)
  • Author(s): Keishiro Fukunaga, Kosaku Kurata, Hiroshi Takamatsu
  • Organizer: Ninth Asian Pacific Conference on Biomechanics
  • Int'l Joint Research
• [Presentation] Effects of Pulse Repetition and Interval on Cell Mortality by Irreversible Electroporation (2016)
  • Author(s): Masahiro Yoshida, Shuto Yoshimatsu, Kosaku Kurata, Hiroshi Takamatsu
  • Organizer: The Fourth International Forum on Heat Transfer
  • Place of Presentation: Sendai
  • Year and Date: 2016-11-02
  • Int'l Joint Research
• [Presentation] Development of comb-shaped miniature electrode for contact irreversible electroporation (2016)
  • Author(s): Shuto Yoshimatsu, Masahiro Yoshida, Kosaku Kurata, Takanobu Fukunaga, Hiroshi Takamatsu
  • Organizer: The 27th International Symposium on Transport Phenomena (ISTP27)
  • Place of Presentation: waikiki
  • Year and Date: 2016-09-20
  • Int'l Joint Research
• [Presentation] 不可逆的電気穿孔による生体擬似組織の電気的特性の変化 (2016)
  • Author(s): 藏田耕作，中村小春，福永鷹信，王海東，高松洋
  • Organizer: 第53回日本伝熱シンポジウム
  • Place of Presentation: 大阪府
  • Year and Date: 2016-05-24
• [Presentation] Irreversible Electroporation: Medical Application of Intense Electric Pulses for Sustainable Health (2015)
  • Author(s): Kosaku Kurata, Hiroshi Takamatsu
  • Organizer: ICE-SEAM 2015
  • Place of Presentation: Solo, Indonesia
  • Year and Date: 2015-11-11
  • Int'l Joint Research / Invited
• [Presentation] 不可逆エレクトロポレーションを利用した非熱的な細胞電壊療法 (2015)
  • Author(s): 藏田耕作
  • Organizer: 九州伝熱セミナーin由布院
  • Place of Presentation: 大分県由布市
  • Year and Date: 2015-11-06
  • Invited
• [Presentation] Contact Irreversible Electroporation for a Less-Invasive Tissue Ablation (2015)
  • Author(s): Kosaku Kurata, Hiroshi Takamatsu
  • Organizer: Twelfth International Conference on Flow Dynamics
  • Place of Presentation: Sendai
  • Year and Date: 2015-10-27
  • Int'l Joint Research
• [Presentation] エレクトロポレーションにおけるパルス印加条件が細胞膜破壊に与える影響 (2015)
  • Author(s): 岡田伸，中村小春，吉松秀隼，吉田将大，福永鷹信，藏田耕作，高松洋
  • Organizer: 第26回バイオフロンティア講演会
  • Place of Presentation: 福岡
  • Year and Date: 2015-10-02
• [Presentation] Feasibility Study on Contact Irreversible Electroporation for Superficial Tumor Treatment by Using Miniature Electrodes (2015)
  • Author(s): Kosaku Kurata, Manabu Sato, Takanobu Fukunaga, Hiroshi Takamatsu
  • Organizer: Eighth Asian Pacific Conference on Biomechanics
  • Place of Presentation: Sapporo
  • Year and Date: 2015-09-16
  • Int'l Joint Research
• [Presentation] エレクトロポレーションによる細胞致死率に及ぼすパルス条件および浸透圧の影響 (2015)
  • Author(s): 吉松秀隼，吉田将大，藏田耕作，福永鷹信，高松洋
  • Organizer: 日本機械学会2015年度年次大会
  • Place of Presentation: 札幌
  • Year and Date: 2015-09-13
• [Presentation] 不可逆エレクトロポレーションによる細胞壊死に関する生体ファントム実験と数値シミュレーションの比較 (2015)
  • Author(s): 吉田将大，吉松秀隼，藏田耕作，福永鷹信，高松洋
  • Organizer: 日本機械学会2015年度年次大会
  • Place of Presentation: 札幌
  • Year and Date: 2015-09-13
• [Presentation] 微小表面電極を用いた接触式不可逆エレクトロポレーションに関する研究 (2015)
  • Author(s): 藏田耕作，佐藤学，福永鷹信，高松洋
  • Organizer: 第51回九大生体材料・力学研究会
  • Place of Presentation: 福岡
  • Year and Date: 2015-07-28
• [Presentation] 不可逆エレクトロポレーションによる細胞壊死領域の評価－生体ファントムを用いた実験と解析の比較－ (2015)
  • Author(s): 吉田将大，吉松秀隼，藏田耕作，福永鷹信，高松洋
  • Organizer: 日本機械学会九州学生会第46回卒業研究発表講演会
  • Place of Presentation: 福岡県北九州市
  • Year and Date: 2015-03-03
• [Presentation] エレクトロポレーションにおけるパルス印加条件が細胞致死率に与える影響 (2015)
  • Author(s): 吉松秀隼，吉田将大，藏田耕作，福永鷹信，高松洋
  • Organizer: 日本機械学会九州学生会第46回卒業研究発表講演会
  • Place of Presentation: 福岡県北九州市
  • Year and Date: 2015-03-03
• [Presentation] Study on contact irreversible electroporation using miniature electrode (2015)
  • Author(s): 佐藤学，藏田耕作，福永鷹信，高松洋
  • Organizer: 第3回バイオメカニクス研究センター＆エレクトロニクス実装学会九州支部合同研究会
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2015-02-06
• [Presentation] 微小表面電極による接触式不可逆エレクトロポレーションの低電圧化に関する研究 (2015)
  • Author(s): 藏田耕作，佐藤学，福永鷹信，高松洋
  • Organizer: 第27回バイオエンジニアリング講演会
  • Place of Presentation: 新潟県新潟市
  • Year and Date: 2015-01-09 – 2015-01-10
• [Presentation] 不可逆エレクトロポレーションにおける電極先端形状が腫瘍除去と熱的損傷に与える影響の解析 (2015)
  • Author(s): 春日陽介，藏田耕作，高松洋
  • Organizer: 第27回バイオエンジニアリング講演会
  • Place of Presentation: 新潟県新潟市
  • Year and Date: 2015-01-09 – 2015-01-10
• [Presentation] Numerical and Experimental Studies on Irreversible Electroporation for Tumor Treatment (2014)
  • Author(s): Kosaku Kurata, Hiroshi Takamatsu
  • Organizer: The 4th Japan-Switzerland Workshop on Biomechanics
  • Place of Presentation: 三重県伊勢市
  • Year and Date: 2014-09-01 – 2014-09-04
• [Presentation] 不可逆エレクトロポレーションによる固形腫瘍の低侵襲治療法に関する研究 (2014)
  • Author(s): 藏田耕作, 高松洋
  • Organizer: 第50回九大生体材料・力学研究会
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2014-08-22
• [Presentation] 不可逆エレクトロポレーションによる非熱的療法 (2014)
  • Author(s): 藏田耕作, 福永鷹信, 高松洋
  • Organizer: 第3回臨床熱工学研究会
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2014-06-16
• [Presentation] 不可逆エレクトロポレーションが生体組織の電気的特性に与える影響 (2014)
  • Author(s): 藏田耕作, 松下将大, 福永鷹信, 高松洋
  • Organizer: 第51回日本伝熱シンポジウム
  • Place of Presentation: 静岡県浜松市
  • Year and Date: 2014-05-21 – 2014-05-23