Multiple functional electron paramagnetic resonance imaging for quantitative cancer studies

• Project/Area Number: 26249057
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Measurement engineering
• Research Institution: Hokkaido University
• Principal Investigator: Hirata Hiroshi 北海道大学, 情報科学研究科, 教授 (60250958)
• Co-Investigator(Kenkyū-buntansha): 安井 博宣 北海道大学, アイソトープ総合センター, 准教授 (10570228)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥41,080,000 (Direct Cost: ¥31,600,000、Indirect Cost: ¥9,480,000); Fiscal Year 2017: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000); Fiscal Year 2016: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000); Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2014: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
• Keywords: 電子スピン共鳴 / 酸素分圧 / pH / 腫瘍 / イメージング / 酸素 / 計測工学 / 癌 / 分析科学 / 放射線 / 可視化 / 電子常磁性共鳴 / 電子スピン共鳴イメージング / 画像再構成 / 緩和時間

Outline of Final Research Achievements

We developed the methods of continues-wave electron spin resonance (ESR) imaging for the visualization of oxygen partial pressure and pH of tumor tissues. Using accelerated four-dimensional spectral-spatial imaging, mapping of oxygen partial pressure and pH performed. ESR imaging recorded relaxation time T2* of oxygen-sensitive spin probes and ESR spectral lines of a pH-sensitive spin probe for oxygen mapping and pH mapping. Estimations of oxygen partial pressure and pH used those spectral parameters. Finally, we tested the developed methods with solution phantoms and tumor-bearing mice.

Research Products

• [Int'l Joint Research] West Virginia University(米国)
• [Int'l Joint Research] Novosibirsk Inst. of Organic Chemistry(ロシア連邦)
• [Int'l Joint Research]
• [Int'l Joint Research] Novosibirsk Inst. Organic Chemistry(ロシア連邦)
• [Int'l Joint Research] West Virginia University/School of Medicine/Department of Biochemistry(米国)
• [Journal Article] Impact of the characteristic impedance of coaxial lines on the sensitivity of a 750-MHz electronically tunable EPR resonator (2018)
  • Author(s): R. Nakaoka, D. A. Komarov, S. Matsumoto, H. Hirata
  • Journal Title: Applied Magnetic Resonance Volume: 印刷中 Issue: 8 Pages: 853-867
  • DOI: 10.1007/s00723-018-1001-6
  • NAID: 120006709282
  • Peer Reviewed
• [Journal Article] A 750-MHz electronically tunable resonator using microstrip line couplers for electron paramagnetic resonance imaging of a mouse tumor-bearing leg (2018)
  • Author(s): T. Amida, R. Nakaoka, D. A. Komarov, K. Yamamoto, O. Inanami, S. Matsumoto, H. Hirata
  • Journal Title: IEEE Transactions on Biomedical Engineering Volume: 65 Pages: 1124-1132
  • DOI: 10.1109/tbme.2017.2743232
  • NAID: 120006466513
  • Peer Reviewed
• [Journal Article] Fast backprojection-based reconstruction of spectral-spatial EPR images from projections with the constant sweep of a magnetic field (2017)
  • Author(s): D. A. Komarov, H. Hirata
  • Journal Title: Journal of Magnetic Resonance Volume: 281 Pages: 44-50
  • DOI: 10.1016/j.jmr.2017.05.005
  • NAID: 120006709274
  • Peer Reviewed
• [Journal Article] Feasibility of in vivo three-dimensional T2* mapping using dicarboxy-PROXYL and CW-EPR-based single-point imaging (2017)
  • Author(s): H. Kubota, D. A. Komarov, H. Yasui, S. Matsumoto, O. Inanami, I. A. Kirilyuk, V. V. Khramtsov, and H. Hirata
  • Journal Title: Magnetic Resonance Materials in Physics, Biology and Medicine Volume: 印刷中 Issue: 3 Pages: 291-298
  • DOI: 10.1007/s10334-016-0606-8
  • NAID: 120006470969
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Passive decoupling due to low Q-factors of four-channel coils in 300-MHz pulsed EPR imaging (2015)
  • Author(s): A. Enomoto, K. Saito, S. Subramanian, M. C. Krishna, H. Hirata, and N. Devasahayam
  • Journal Title: Applied Magnetic Resonance Volume: - Issue: 6 Pages: 671-683
  • DOI: 10.1007/s00723-015-0671-6
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Three-dimensional oxygen mapping using a pair of isotopic nitroxyl radicals and CW-EPR-based single-point imaging (2017)
  • Author(s): H. Kubota, D. A. Komarov, S. Matsumoto, K. Yamamoto, H. Yasui, O. Inanami, I. A. Kirilyuk, V. V. Khramtsov, H. Hirata
  • Organizer: EPR 2017: International Conference on Electron Paramagnetic Resonance Spectroscopy and Imaging of Biological Systems
  • Int'l Joint Research / Invited
• [Presentation] Fast backprojection-based reconstruction of spectral-spatial EPR images from projections with the constant sweep of a magnetic field (2017)
  • Author(s): D. A. Komarov, H. Hirata
  • Organizer: EPR 2017: International Conference on Electron Paramagnetic Resonance Spectroscopy and Imaging of Biological Systems
  • Int'l Joint Research
• [Presentation] 窒素同位体標識ニトロキシルラジカルによる腫瘍モデルマウスの酸素分圧イメージング (2017)
  • Author(s): 久保田晴江, 安井博宣, 松元慎吾, 稲波修, Igor A. Kirilyuk, Valery V. Khramtsov, 平田拓
  • Organizer: 第56回電子スピンサイエンス学会年会
• [Presentation] 電子常磁性共鳴分光による腫瘍組織のpHマッピング法の開発 (2017)
  • Author(s): 市川裕貴, Denis A. Komarov, 山本久美子, 稲波修, 松元慎吾, 平田拓
  • Organizer: 第56回電子スピンサイエンス学会年会
• [Presentation] 腫瘍モデルマウス用750MHzチューナブル共振器の最適設計 (2017)
  • Author(s): 中岡梨々子, 平田拓
  • Organizer: 第56回電子スピンサイエンス学会年会
• [Presentation] マイクロ波シミュレーションによる750 MHz EPR共振器の磁場発生効率の最適化 (2016)
  • Author(s): 編田竜也, 松元慎吾, 平田拓
  • Organizer: 第55回電子スピンサイエンス学会年会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2016-11-10
• [Presentation] 高速EPRシングル・ポイント・イメージング法における部分k空間データ取得の緩和時間T2*マッピングへの影響評価 (2016)
  • Author(s): 長谷川昴平, 松元慎吾, 平田拓
  • Organizer: 第55回電子スピンサイエンス学会年会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2016-11-10
• [Presentation] EPR分光によるpH推定アルゴリズムの開発 (2016)
  • Author(s): 市川裕貴, 平田拓
  • Organizer: 第55回電子スピンサイエンス学会年会
  • Place of Presentation: 大阪市立大学
  • Year and Date: 2016-11-10
• [Presentation] CW-EPR法による酸素分圧及びプローブ濃度定量化計測の実現可能性検証 (2016)
  • Author(s): 安田雄介, 久保田晴江, 松元慎吾, 平田拓
  • Organizer: 第54回電子スピンサイエンス学会年会
  • Place of Presentation: 朱鷺メッセ（新潟コンベンションセンター）
  • Year and Date: 2016-11-02
• [Presentation] 部分k空間データ取得による高速EPRシングル・ポイント・イメージング法の実験 (2016)
  • Author(s): 長谷川昴平, 松元慎吾, 平田拓
  • Organizer: 第54回電子スピンサイエンス学会年会
  • Place of Presentation: 朱鷺メッセ（新潟コンベンションセンター）
  • Year and Date: 2016-11-02
• [Presentation] マイクロストリップラインの結合を利用して共振周波数の調整を行う750MHz EPR共振器の開発 (2016)
  • Author(s): 編田竜也, 平田拓
  • Organizer: 第54回電子スピンサイエンス学会年会
  • Place of Presentation: 朱鷺メッセ（新潟コンベンションセンター）
  • Year and Date: 2016-11-02
• [Presentation] 酸素感受性同位体ニトロキシルラジカルの特性評価実験 (2016)
  • Author(s): 久保田晴江, 安井博宣, 松元慎吾, 三宅祐輔, 稲波修, I. A. Kirilyuk, V. V. Khramtsov, 平田拓
  • Organizer: 第54回電子スピンサイエンス学会年会
  • Place of Presentation: 朱鷺メッセ（新潟コンベンションセンター）
  • Year and Date: 2016-11-02
• [Presentation] CW-EPR-based oxygen mapping technique using a pair of isotopic nitroxyl radicals (2016)
  • Author(s): H. Kubota, H. Yasui, S. Matsumoto, O. Inanami, I. A. Kirilyuk, V. V. Khramtsov, H. Hirata
  • Organizer: Xth International Workshop on EPR in Biology and Medicine
  • Place of Presentation: Krakow, Poland
  • Year and Date: 2016-10-02
  • Int'l Joint Research / Invited
• [Presentation] Development of a CW-EPR-based oxygen-mapping technique using a pair of isotopic nitroxyl radicals (2016)
  • Author(s): H. Hirata, H. Kubota, H. Yasui, S. Matsumoto, O. Inanami, I. A. Kirilyuk, and V. V. Khramtsov
  • Organizer: Asia-Pacific EPR/ESR Symposium 2016
  • Place of Presentation: Irkutsk, Russia
  • Year and Date: 2016-08-28
  • Int'l Joint Research / Invited
• [Presentation] Feasibility study of a CW-EPR-based oxygen mapping technique using a pair of isotopic nitroxyl radicals (2016)
  • Author(s): H. Hirata, H. Kubota, H. Yasui, S. Matsumoto, O. Inanami, I. A. Kirilyuk, and V. V. Khramtsov
  • Organizer: 58th Annual Rocky Mountain Conference on Magnetic Resonance (39th International EPR Symposium)
  • Place of Presentation: Breckenridge, Colorado, USA
  • Year and Date: 2016-07-17
  • Int'l Joint Research
• [Presentation] Characteristics of 14N- and 15N-labeled dicarboxy-PROXYLs as oxygen-sensitive probes for CW-EPR-based single-point imaging (SPI) (2016)
  • Author(s): H. Kubota, H. Yasui, S. Matsumoto, O. Inanami, I. A. Kirilyuk, V. V. Khramtsov, and H. Hirata
  • Organizer: 58th Annual Rocky Mountain Conference on Magnetic Resonance (39th International EPR Symposium)
  • Place of Presentation: Breckenridge, Colorado, USA
  • Year and Date: 2016-07-17
  • Int'l Joint Research
• [Presentation] Simulation of RF magnetic fields in crossed loop resonators for 300 MHz pulsed EPR (2014)
  • Author(s): T. Ishii and H. Hirata
  • Organizer: Joint Conference of 9th Asia-Pacific EPR/ESR Symposium, 1st International EPR (ESR) Society Symposium, and 53rd SEST Annual Meeting (APES-IES-SEST 2014)
  • Place of Presentation: Nara, Japan
  • Year and Date: 2014-11-12 – 2014-11-16
• [Presentation] Artifact suppression in electron paramagnetic resonance imaging of 14N- and 15N-labeled nitroxyl radicals with asymmetric absorption spectra (2014)
  • Author(s): W. Takahashi, Y. Miyake, and H. Hirata
  • Organizer: Joint Conference of 9th Asia-Pacific EPR/ESR Symposium, 1st International EPR (ESR) Society Symposium, and 53rd SEST Annual Meeting (APES-IES-SEST 2014)
  • Place of Presentation: Nara, Japan
  • Year and Date: 2014-11-12 – 2014-11-16
• [Presentation] Electron paramagnetic resonance spectroscopy for in vivo measurement of tumour extracellular pH- the effect of X-ray irradiation (2014)
  • Author(s): J. Goodwin, K. Yachi, M. Nagane, H. Yasui, Y. Miyake, O. Inanami, A. Bobko, V. Khramstov, H. Hirata
  • Organizer: Joint Annual Meeting ISMRM-ESMRMB 2014
  • Place of Presentation: Milan, Italy
  • Year and Date: 2014-05-10 – 2014-05-16