Elucidating mechanisms of activated sludge reduction by a high-pressure jet device

• Project/Area Number: 26249076
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Civil and environmental engineering
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Hosomi Masaaki 東京農工大学, 工学(系)研究科(研究院), 教授 (90132860)
• Co-Investigator(Kenkyū-buntansha): 堀 知行 国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 研究グループ付 (20509533) ; 寺田 昭彦 東京農工大学, 工学(系)研究科(研究院), 教授 (30434327)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥33,150,000 (Direct Cost: ¥25,500,000、Indirect Cost: ¥7,650,000); Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2014: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
• Keywords: 高圧ジェット装置 / 汚泥減容化 / 活性汚泥 / 圧壊 / 排水処理 / 16S rRNA / 酸素富化 / 余剰汚泥減容化 / 硝化液循環活性汚泥法 / 活性汚泥法 / 微生物叢解析 / コスト試算 / 細菌 / 窒素除去 / 高度処理 / 酸素富加 / 余剰汚泥減量化 / 細菌破砕 / 微生物群集構造解析 / 汚泥減溶化 / 細胞損傷 / 細胞膜 / 細胞壁 / 糸状性細菌 / キャビテーション

Outline of Final Research Achievements

To reduce the amount of excess sludge from a wastewater treatment facility, a high-pressure jet device (HPJD) was developed. Bacterial cell disruption mechanisms by an HPJD were investigated. The application of an atomic force microscopy to a single bacterial cell elucidated that cavitation is most intense factor to disrupt bacterial cells among friction, collision and itself. The installation of an HPJD into a modified Ludzak-Ettinger process reduced the amount of excess sludge discharge by, at maximum, 73% in comparison with the process without an HPJD. Deterioration of water qualities of the treated water and settlability of activated sludge due to an HPJD was not observed. The analysis of amplicon sequencing based on 16S rRNA gene displayed the reduction of filamentous bacteria in the process with an HPJD, suggesting an effectiveness in prevention of sludge bulking.

Research Products

• [Journal Article] Disentangling the multiple effects of a novel high pressure jet device upon bacterial cell disruption (2017)
  • Author(s): Xie Li、Terada Akihiko、Hosomi Masaaki
  • Journal Title: Chemical Engineering Journal Volume: 323 Pages: 105-113
  • DOI: 10.1016/j.cej.2017.04.067
  • Peer Reviewed
• [Journal Article] Efficacy of a high-pressure jet device for excess sludge reduction in a conventional activated sludge process: Pilot-scale demonstration (2017)
  • Author(s): Yoshino Hiroyuki、Suenaga Toshikazu、Fujii Tadahiro、Hori Tomoyuki、Terada Akihiko、Hosomi Masaaki
  • Journal Title: Chemical Engineering Journal Volume: 326 Pages: 78-86
  • DOI: 10.1016/j.cej.2017.05.084
  • Peer Reviewed
• [Journal Article] Single-cell analysis of the disruption of bacteria with a high-pressure jet device: An application of atomic force microscopy (2016)
  • Author(s): L. Xie, Q. Bao, A. Terada, M. Hosomi
  • Journal Title: Chemical Engineering Journal Volume: 306 Pages: 1099-1108
  • DOI: 10.1016/j.cej.2016.07.112
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] High-pressure jet device for activated sludge reduction: Feasibility of sludge solubilization (2015)
  • Author(s): Suenaga, T., Nishimura, M., Yoshino, H., Kato, H., Nonokuchi, M., Fujii, T., Satoh, H., Terada, A., Hosomi, M.
  • Journal Title: Biochemical Engineering Journal Volume: 100 Pages: 1-8
  • DOI: 10.1016/j.bej.2015.03.022
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of a predominant effect on bacterial cell disruption and released organic matters by a high-pressure jet device (2015)
  • Author(s): Xie, L., Qian, B., Suenaga, T., Yoshino H., Terada, A., Hosomi, M.
  • Journal Title: Biochemical Engineering Journal Volume: 101 Pages: 220-227
  • DOI: 10.1016/j.bej.2015.05.019
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 高圧ジェット装置を導入した硝化液循環活性汚泥法における余剰汚泥減容化 (2018)
  • Author(s): 吉野 寛之、堀 知行、寺田 昭彦、細見 正明
  • Organizer: 化学工学会 第83年会
• [Presentation] 活性汚泥法への高圧噴射装置の導入による細菌・真核生物群集構造変化の追跡 (2017)
  • Author(s): 吉野寛之、末永俊和、藤井忠弘、堀知行、寺田昭彦、細見正明
  • Organizer: 第５１回日本水環境学会年会
  • Place of Presentation: 熊本大学（熊本）
  • Year and Date: 2017-03-15
• [Presentation] Elucidating a dominant effect of high-pressure jet device on bacterial cell disruption and release of intracellular substances (2016)
  • Author(s): L. Xie, A. Terada, M. Hosomi
  • Organizer: 1st International Conference on Bioresource Technology
  • Place of Presentation: バルセロナ（スペイン）
  • Year and Date: 2016-10-23
  • Int'l Joint Research
• [Presentation] Feasibility of a high pressure jet device for excess activity sludge reduction and selective bacterial disruption towards microbial community control (2016)
  • Author(s): H. Yoshino, T. Fujii, T. Hori, A. Terada, M. Hosomi
  • Organizer: MEWE and Biofilms IWA Specialist Conference
  • Place of Presentation: コペンハーゲン（デンマーク）
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] 高圧噴射装置を導入した循環式硝化脱窒法における余剰汚泥減容化 (2016)
  • Author(s): 湊拓弘、吉野寛之、寺田昭彦、藤井忠弘、細見正明
  • Organizer: 第50回 日本水環境学会年会
  • Place of Presentation: アスティ徳島（徳島県徳島市）
  • Year and Date: 2016-03-16
• [Presentation] 高圧噴射装置の導入による糸状性細菌の発生に起因するバルキングの抑制 (2016)
  • Author(s): 吉野寛之、藤井忠弘、堀知行、寺田昭彦、細見正明
  • Organizer: 第50回 日本水環境学会年会
  • Place of Presentation: アスティ徳島（徳島県徳島市）
  • Year and Date: 2016-03-16
• [Presentation] Elucidating a predominant effect on bacterial cell disruption by a high pressure jet device and released dissolved organic matters (2015)
  • Author(s): Li Xie, Wian Bao, Toshikazu Suenaga, Hiroyuki Yoshino, Akihiko Terada, Masaaki Hosomi
  • Organizer: 6th IWA-ASPIRE Conference & Exhibition
  • Place of Presentation: Beijing, China
  • Year and Date: 2015-09-20 – 2015-09-24
• [Presentation] 高圧噴射装置の設置による余剰汚泥減容化とそれに伴う微生物群集の変化 (2015)
  • Author(s): 吉野寛之、藤井忠弘、堀知行、寺田昭彦、細見正明
  • Organizer: 化学工学会第47回秋季大会
  • Place of Presentation: 北海道大学（北海道札幌市）
  • Year and Date: 2015-09-09
• [Presentation] 高圧噴射装置による余剰汚泥減容化に伴う細菌・真核生物群集構造の変化 (2015)
  • Author(s): 吉野寛之、藤井忠弘、堀知行、寺田昭彦、細見正明
  • Organizer: 環境バイオテクノロジー学会2015年度大会
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-06-29
• [Presentation] Pilot-scale demonstration of a high pressure jet device for excess sludge reduction from a conventional activated sludge system (2015)
  • Author(s): Akihiko Terada, Hiroyuki Yoshino, Li Xie, Toshikazu Suenaga, Tomoyuki Hori, Shohei Riya, Masaaki Hosomi
  • Organizer: EnvironmentAsia 2015
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-06-17 – 2015-06-19
  • Invited
• [Presentation] Pilot-scale demonstration of a high pressure jet device for excess sludge reduction from a conventional activated sludge system (2015)
  • Author(s): Terada, A., Yoshino, H., Xie, L., Suenaga, T., Hori, T., Riya, S., Hosomi, M.
  • Organizer: The 3rd EnvironmentAsia conference
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2015-06-17
  • Int'l Joint Research / Invited
• [Presentation] Sludge reduction and microbial community of activated sludge system with a high pressure jet device (2015)
  • Author(s): Yoshino, H., Suenaga, T., Hori, T., Terada, A., Fujii, T., Nonokuchi, M., Hosomi, M.
  • Organizer: The 12th IWA Leading Edge Conference on Water and Wastewater Technologies
  • Place of Presentation: Hong Kong, China
  • Year and Date: 2015-05-30
  • Int'l Joint Research
• [Presentation] Characteristics of bacterial cell destruction and dissolved organic matter by a high pressure jet device (2015)
  • Author(s): Li Xie, Wian Bao, Toshikazu Suenaga, Hiroyuki Yoshino, Akihiko Terada, Masaaki Hosomi
  • Organizer: 化学工学会 第80年会
  • Place of Presentation: 東京都江東区（芝浦工業大学）
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] 高圧ジェット装置による余剰汚泥減容化に伴う細菌・真核生物群集構造の変化 (2015)
  • Author(s): 吉野寛之、藤井忠弘、堀智行、寺田昭彦、細見正明
  • Organizer: 第49回日本水環境学会年会
  • Place of Presentation: 金沢大学（石川県金沢市）
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 高圧ジェット装置による余剰汚泥減容が微生物叢の変化と排水処理性能に及ぼす影響 (2014)
  • Author(s): 吉野寛之、寺田昭彦、藤井忠弘、堀知行、野々口稔、細見正明
  • Organizer: 環境科学会2014年会
  • Place of Presentation: つくば国際会議場（茨城県つくば市）
  • Year and Date: 2014-09-18 – 2014-09-19
• [Presentation] Performance of excess activated sludge reduction and the resultant microbial community structure by a high pressure jet device (2014)
  • Author(s): Hiroyuki Yoshino, Toshikazu Suenaga, Akihiko Terada, Tadhiro Fujii, Minoru Nonoguchi, Masaaki Hosomi
  • Organizer: Water and Environment Technology Conference 2014
  • Place of Presentation: 早稲田大学（東京都新宿区）
  • Year and Date: 2014-06-28 – 2014-06-29