Fundamental research for genome-wide platform of industrial mammalian cells
| Project/Area Number |
26249125
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka University (2015-2017)
The University of Tokushima (2014) |
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Principal Investigator |
Omasa Takeshi 大阪大学, 工学研究科, 教授 (00252586)
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| Co-Investigator(Kenkyū-buntansha) |
西島 謙一 名古屋大学, 工学研究科, 准教授 (10262891)
河原 正浩 東京大学, 大学院工学系研究科(工学部), 准教授 (50345097)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
Fiscal Year 2017: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2016: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2015: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2014: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
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| Keywords | CHO cell / mammalian cell / antibody production / genome-wide analysis / chromosome / バイオ医薬品 / チャイニーズハムスター卵巣細胞 / ゲノム育種 / 抗体医薬 / ﾊﾞｲｵ医薬品 / ｹﾞﾉﾑ育種 |
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| Outline of Final Research Achievements |
The fundamental research of chromosome stability and selection of targeting region of genome, and analysis of signaling pathway for Chinese hamster ovary cells are necessary for genome-wide platform of industrial mammalian cells. We focused on the chromosome stability and find that BRCA1 repression is effective for the construction of stable and productive cell lines. Moreover, we investigated genome-wide gene expression among the CHO cell lines which have various chromosome numbers and determined the differentially-expressed genes among these cell lines and estimated the functions. Also, we determined the suitable target chromosomal region for site-specific genome integration.
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Report
(4 results)
Research Products
(68 results)
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[Presentation] Overview of Biologics Production in Japan2015
Author(s)
Takeshi Omasa
Organizer
2nd Joint International Symposium on Animal Cell Technology for Asian Network by JAACT, TCELS and MU
Place of Presentation
Bangkok, Thailand
Year and Date
2015-11-18
Related Report
Int'l Joint Research / Invited
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