| Budget Amount *help |
¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
Fiscal Year 2017: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2016: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2015: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2014: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
|
|---|
| Outline of Final Research Achievements |
The fundamental research of chromosome stability and selection of targeting region of genome, and analysis of signaling pathway for Chinese hamster ovary cells are necessary for genome-wide platform of industrial mammalian cells. We focused on the chromosome stability and find that BRCA1 repression is effective for the construction of stable and productive cell lines. Moreover, we investigated genome-wide gene expression among the CHO cell lines which have various chromosome numbers and determined the differentially-expressed genes among these cell lines and estimated the functions. Also, we determined the suitable target chromosomal region for site-specific genome integration.
|
