| Budget Amount *help |
¥41,080,000 (Direct Cost: ¥31,600,000、Indirect Cost: ¥9,480,000)
Fiscal Year 2016: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2014: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
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| Outline of Final Research Achievements |
Comprehensive connectivity maps of nervous systems and connectomics are providing detailed designs of neuronal circuits. Although these connectivity maps are powerful in predicting hypothetical circuit functions and guiding new experiments thereon, the information of neurotransmitters and receptors is critical in interpreting how a given circuit functions. This project research aims at comprehensive mapping of neurotransmitters in the Drosophila brain. Using the CRISPR/Cas9 system, we generated a transgenic library of GAL4 knock-in that recapitulate the expression of genes for neurotransmitter biosynthesis and their receptors. By visualizing their expression patterns in the brain, we quantitatively compare the distribution of neurotransmitter-releasing and receiving cells. This study provided the basis of neurotransmitter map in the fly brain.
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