Direct visualization of endogenous gene expression through targeted genetic labeling

• Project/Area Number: 26250001
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Tohoku University
• Principal Investigator: Tanimoto Hiromu 東北大学, 生命科学研究科, 教授 (70714955)
• Co-Investigator(Kenkyū-buntansha): 近藤 周 国立遺伝学研究所, 系統生物研究センター, 助教 (90408401)
• Research Collaborator: YAMAGATA Nobuhiro 東北大学, 大学院生命科学研究科, 助教 (50716248)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥41,080,000 (Direct Cost: ¥31,600,000、Indirect Cost: ¥9,480,000); Fiscal Year 2016: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2014: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
• Keywords: 脳機能モデル動物 / 遺伝学 / 神経科学

Outline of Final Research Achievements

Comprehensive connectivity maps of nervous systems and connectomics are providing detailed designs of neuronal circuits. Although these connectivity maps are powerful in predicting hypothetical circuit functions and guiding new experiments thereon, the information of neurotransmitters and receptors is critical in interpreting how a given circuit functions. This project research aims at comprehensive mapping of neurotransmitters in the Drosophila brain. Using the CRISPR/Cas9 system, we generated a transgenic library of GAL4 knock-in that recapitulate the expression of genes for neurotransmitter biosynthesis and their receptors. By visualizing their expression patterns in the brain, we quantitatively compare the distribution of neurotransmitter-releasing and receiving cells. This study provided the basis of neurotransmitter map in the fly brain.

Research Products

• [Int'l Joint Research] Max Planck Institute of Neurobiology/University of Munich(Germany)
• [Int'l Joint Research] HHMI Janelia Research Campus/Cold Spring Harbor Laboratory(米国)
• [Int'l Joint Research] Max Planck Institute of Neurobiology/University of Munich/Leibniz Institute for Neurobiology(Germany)
• [Int'l Joint Research] HHMI Janelia Research Campus/Cold Spring Harbor Laboratory(米国)
• [Journal Article] Suppression of dopamine neurons mediates reward. (2016)
  • Author(s): Yamagata N, Hiroi M, Kondo S, Abe A, Tanimoto, H.
  • Journal Title: PLoS Biol Volume: 14(12) Issue: 12 Pages: e1002586-e1002586
  • DOI: 10.1371/journal.pbio.1002586
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Dynamics of memory-guided choice behavior in <i>Drosophila</i> (2016)
  • Author(s): Rohwedder A, Wenz NL, Stehle B, Huser A, Yamagata N, Zlatic M, Truman JW, Tanimoto H, Saumweber T, Gerber B.
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 92 Issue: 8 Pages: 346-357
  • DOI: 10.2183/pjab.92.346
  • NAID: 130005420972
  • ISSN: 0386-2208, 1349-2896
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Direct neural pathways convey distinct visual information to Drosophila mushroom bodies. (2016)
  • Author(s): Vogt K, Aso Y, Hige T, Knapek S, Ichinose T, Friedrich AB, Turner GC, Rubin GM, Tanimoto H.
  • Journal Title: eLife Volume: in press
  • DOI: 10.7554/elife.14009
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Functional dissociation in sweet taste receptor neurons between and within taste or- gans of Drosophila. (2016)
  • Author(s): Thoma V, Knapek S, Arai S, Hartl M, Kohsaka H, Sirigrivatanawong P, Abe A, Hashimoto K, Tanimoto H.
  • Journal Title: Nature Communications Volume: 7 Issue: 1 Pages: 10678-10678
  • DOI: 10.1038/ncomms10678
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Reward signal in a recurrent circuit drives appetitive long-term memory formation. (2015)
  • Author(s): Ichinose T, Aso Y, Yamagata N, Abe A, Rubin GM, Tanimoto H.
  • Journal Title: eLife Volume: 4 Pages: 1-18
  • DOI: 10.7554/elife.10719
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Reversing Stimulus Timing in Visual Conditioning Leads to Memories with Opposite Valence in Drosophila. (2015)
  • Author(s): Vogt K, Yarali A, Tanimoto H.
  • Journal Title: PLoS One Volume: 10(10) Issue: 10 Pages: e0139797-e0139797
  • DOI: 10.1371/journal.pone.0139797
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A model for non-monotonic intensity coding. (2015)
  • Author(s): Nehrkorn J, Tanimoto H, Herz AV, Yarali A.
  • Journal Title: R Soc Open Sci Volume: 2 Issue: 5 Pages: 150120-150120
  • DOI: 10.1098/rsos.150120
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genome-Wide Association Analyses Point to Candidate Genes for Electric Shock Avoidance in Drosophila melanogaster. (2015)
  • Author(s): Appel M, Scholz CJ, Muller T, Dittrich M, Konig C, Bockstaller M, Oguz T, Khalili A, Antwi-Adjei E, Schauer T, Margulies C, Tanimoto H, Yarali A.
  • Journal Title: PLoS One Volume: 10 Issue: 5 Pages: e0126986-e0126986
  • DOI: 10.1371/journal.pone.0126986
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distinct dopamine neurons mediate reward signals for short- and long-term memories (2015)
  • Author(s): Yamagata N, Ichinose T, Aso Y, Placais P, Friedrich AB, Sima RJ, Preat T, Rubin GM, Tanimoto H
  • Journal Title: PNAS Volume: 112 Issue: 2 Pages: 578-583
  • DOI: 10.1073/pnas.1421930112
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mushroom body output neurons encode valence and guide memory-based action selection in Drosophila (2014)
  • Author(s): Aso Y, Sitaraman D, Ichinose T, Kaun KR, Vogt K, Belliart-Guerin G, Placais PY, Robie AA, Yamagata N, Schnaitmann C, Rowell WJ, Johnston RM, Ngo TT, Chen N, Korff W, Nitabach MN, Heberlein U, Preat T, Branson KM, Tanimoto H, Rubin GM
  • Journal Title: Elife Volume: 4
  • DOI: 10.7554/elife.04580
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The neuronal architecture of the mushroom body provides a logic for associative learning. (2014)
  • Author(s): Aso Y, Hattori D, Yu Y, Johnston R, Iyer N, Ngo T, Dionne H, Abbott L, Axel R, Tanimoto H, Rubin G.
  • Journal Title: eLife Volume: 3
  • DOI: 10.7554/elife.04577
  • Peer Reviewed / Open Access
• [Journal Article] Shared mushroom body circuits operate visual and olfactory memories in Drosophila. (2014)
  • Author(s): Vogt K, Schnaitmann C, Dylla K, Knapek S, Aso Y, Rubin GM, Tanimoto H.
  • Journal Title: eLife Volume: 3
  • Peer Reviewed / Open Access
• [Presentation] Visualization and neuronal control of memory-guided choice behaviour. (2017)
  • Author(s): Tanimoto H.
  • Organizer: Janelia Farm Conference “Structure and Function of the Insect Mushroom Body”
  • Place of Presentation: Verginia, USA
  • Year and Date: 2017-03-05
  • Int'l Joint Research / Invited
• [Presentation] Neural pathways for the formation, consolidation, and retrieval of memory in the fly brain. (2016)
  • Author(s): Tanimoto H.
  • Organizer: The 6th International Symposium on "Biology of Decision Making"
  • Place of Presentation: Paris, France
  • Year and Date: 2016-05-25
  • Int'l Joint Research / Invited
• [Presentation] Dopamine circuits and memory formation. (2015)
  • Author(s): Tanimoto H.
  • Organizer: 14th European Symposium for Insect Taste and Olfaction
  • Place of Presentation: Cagliari, Italy
  • Year and Date: 2015-09-20
  • Int'l Joint Research / Invited
• [Presentation] Mushroom body reward circuits. (2015)
  • Author(s): Tanimoto H.
  • Organizer: The EMBO-Kavli meeting “Neural circuits and behaviour of Drosophila”
  • Place of Presentation: Crete, Greece
  • Year and Date: 2015-07-05
  • Int'l Joint Research / Invited
• [Presentation] Dopamine circuits for memory formation. (2015)
  • Author(s): Tanimoto H.
  • Organizer: 3rd Asia-Pacific Drosophila Research Conference
  • Place of Presentation: Beijing, China
  • Year and Date: 2015-05-11
  • Int'l Joint Research / Invited
• [Presentation] Mashroom body circuits and memory formation. (2014)
  • Author(s): Tanimoto H.
  • Organizer: The Genetics Society Autumn Meeting 2014
  • Place of Presentation: The Royal Society, London (UK)
  • Year and Date: 2014-11-27 – 2014-11-28
  • Invited
• [Presentation] Mashroom body circuits and memory formation. (2014)
  • Author(s): Tanimoto H.
  • Organizer: Neurofly 2014
  • Place of Presentation: Convention Center Crete Maris, Crete (Greece)
  • Year and Date: 2014-10-05 – 2014-10-09
  • Invited
• [Presentation] Distinct dopamine neurons mediate reward signals for short- and long-term memories. (2014)
  • Author(s): Tanimoto H.
  • Organizer: Janelia Farm 2014 Fall Conference
  • Place of Presentation: Janelia Farm Reserch Campus, Ashburn (USA)
  • Year and Date: 2014-09-21 – 2014-09-24
  • Invited
• [Presentation] Neural circuits for memory formation. (2014)
  • Author(s): Tanimoto H.
  • Organizer: The 85th Annual Meeting of the Zoological Society of Japan
  • Place of Presentation: Tohoku University Kawauchi Campus, Sendai
  • Year and Date: 2014-09-11 – 2014-09-13
  • Invited
• [Presentation] Neural circuits for colour discrimination and learning. (2014)
  • Author(s): Tanimoto H, Vogt K, Schnaitmann C, Aso Y, Rubin GM, Wachtler T, Garbers C.
  • Organizer: 11th International Congress of Neuroethology and 36th Annual Meeting of The Japanese Society for Comparative Physiology and Biochemistry
  • Place of Presentation: Sapporo Convention Center, Sapporo
  • Year and Date: 2014-07-29 – 2014-08-01
  • Invited
• [Presentation] Functional diversity of the Drosophila mushroom body. (2014)
  • Author(s): Tanimoto H.
  • Organizer: Janelia Farm 2014 Spring Conference
  • Place of Presentation: Janelia Farm Reserch Campus, Ashburn (USA)
  • Year and Date: 2014-04-27 – 2014-05-01
  • Invited