Exploring the brain circuit principles using nerve fiber imaging

• Project/Area Number: 26250003
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: The University of Tokyo
• Principal Investigator: Ikegaya Yuji 東京大学, 大学院薬学系研究科(薬学部), 教授 (10302613)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥39,910,000 (Direct Cost: ¥30,700,000、Indirect Cost: ¥9,210,000); Fiscal Year 2017: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2016: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2015: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
• Keywords: 神経線維 / 樹状突起 / 軸索 / カルシウム / スパイン / 脳・神経 / 神経細胞 / イメージング / 神経科学

Outline of Final Research Achievements

We devised and improved the optical system, which now allows us to image over 300 spines at a high speed of 100 Hz. Using this new technology:
i) we captured dendritic computations of pyramidal cells of the Hippocampal CA3 field. Large-scale analyses of the dendritic computations in terms of the circuit operation principle revealed that the balance between excitatory and inhitibory inputs collapsed locally. We published these results in Cell Reports.
ii) Hippocampal CA1 neurons that had been activated by environmental exploration were reactivated by specific synchronous inputs from precisely woven neuronal circuits in the upstream CA3 field. Using optical time-lapse imaging of dendrites, we discovered that the precision of cells to be activated gradually increases by repeating the synchronous activity itself. We published these results in Science.

Research Products

• [Journal Article] Hippocampal ripples down-regulate synapses (2018)
  • Author(s): Norimoto Hiroaki、Makino Kenichi、Gao Mengxuan、Shikano Yu、Okamoto Kazuki、Ishikawa Tomoe、Sasaki Takuya、Hioki Hiroyuki、Fujisawa Shigeyoshi、Ikegaya Yuji
  • Journal Title: Science Volume: 359 Issue: 6383 Pages: 1524-1527
  • DOI: 10.1126/science.aao0702
  • Peer Reviewed
• [Journal Article] Activation of Hilar Mossy Cells and Dentate Granule Cells During Sharp Wave/Ripples (2017)
  • Author(s): Ikegaya Yuji、Ouchi Ayako、Matsumoto Nobuyoshi
  • Journal Title: Curr. Res. Neurosci. Volume: 7 Issue: 1 Pages: 1-8
  • DOI: 10.3923/crn.2017.1.8
  • Peer Reviewed / Open Access
• [Journal Article] Functional organization of flash-induced V1 offline reactivation (2016)
  • Author(s): Funayama, K., Hagura, N., Ban, H., Ikegaya, Y.
  • Journal Title: J. Neurosci. Volume: 36 Issue: 46 Pages: 11727-11738
  • DOI: 10.1523/jneurosci.1575-16.2016
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 3-Hz subthreshold oscillations of CA2 neurons in vivo (2016)
  • Author(s): Matsumoto, N. Okamoto, K., Takagi, Y., Ikegaya, Y.
  • Journal Title: Hippocampus Volume: 26 Issue: 12 Pages: 1570-1578
  • DOI: 10.1002/hipo.22657
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Subcellular imbalances in synaptic activity (2016)
  • Author(s): Takahashi, N., Kobayashi, C., Ishikawa, T., Ikegaya, Y
  • Journal Title: Cell Reports Volume: 14 Issue: 6 Pages: 1348-1354
  • DOI: 10.1016/j.celrep.2016.01.024
  • Peer Reviewed / Open Access
• [Journal Article] cAMP-dependent calcium oscillations of astrocytes: an implication for pathology. (2016)
  • Author(s): Ujita, S., Sasaki, T., Asada, A., Funayama, K., Gao, M., Mikoshiba, K., Matsuki, N., Ikegaya, Y.
  • Journal Title: Cereb. Cortex Volume: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Spatial clusters of constitutively active neurons in mouse visual cortex. (2016)
  • Author(s): Makino, K., Funayama, K., Ikegaya, Y.
  • Journal Title: Anat. Sci. Int. Volume: 91 Issue: 2 Pages: 188-195
  • DOI: 10.1007/s12565-015-0284-z
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Subtle modulation of ongoing calcium dynamics in astrocytic microdomains by sensory inputs (2015)
  • Author(s): Asada, A., Ujita, S., Nakayama, R., Oba, S., Ishii, S., Matsuki, N., Ikegaya, Y.
  • Journal Title: Physiol. Rep. Volume: 3 Issue: 10 Pages: e12454-e12454
  • DOI: 10.14814/phy2.12454
  • NAID: 120005844257
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Memory formation and retrieval of neuronal silencing in the auditory cortex. (2015)
  • Author(s): H. Nomura*+, K. Hara, R. Abe, N. Hitora-Imamura, R. Nakayama, T. Sasaki*, N. Matsuki, Y. Ikegaya*
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 112 Issue: 31 Pages: 9740-9744
  • DOI: 10.1073/pnas.1500869112
  • Peer Reviewed
• [Journal Article] Ex vivo cultured neuronal networks emit in vivo-like spontaneous activity (2014)
  • Author(s): Okamoto K, Ishikawa T, Abe R, Ishikawa D, Kobayashi C, Mizunuma M, Norimoto N, Matsuki N and Ikegaya Y
  • Journal Title: Journal of Physiological Sciences Volume: 64 Issue: 6 Pages: 421-31
  • DOI: 10.1007/s12576-014-0337-4
  • Peer Reviewed
• [Journal Article] Sound-induced hyperpolarization of hippocampal neurons. (2014)
  • Author(s): Abe, R., Sakaguchi, T., Matsumoto, N., Matsuki, N., and Ikegaya, Y.
  • Journal Title: Neuroreport. Volume: 25 Issue: 13 Pages: 1013-1017
  • DOI: 10.1097/wnr.0000000000000206
  • Peer Reviewed / Open Access
• [Presentation] Sharp Wave発生時における空間的に局在したシナプス入力 (2017)
  • Author(s): 石川智愛、池谷裕二
  • Organizer: 第40回日本神経科科学大会
• [Presentation] CA1 pyramidal neurons receive spatially clustered synaptic inputs during sharp waves/ripples EX vivo (2017)
  • Author(s): Ishikawa, T., Ikegaya, Y.
  • Organizer: Society for Neuroscience 2017
• [Presentation] シナプス入力の大規模イメージグ、シンポジウム：高速・大規模データ取得のための顕微鏡技術 (2014)
  • Author(s): 池谷裕二
  • Organizer: 日本顕微鏡学会第70回記念学術講演会
  • Place of Presentation: 幕張
  • Year and Date: 2014-05-11 – 2014-05-13
  • Invited
• [Remarks] 東京大学・大学院薬学系研究科・薬品作用学教室のホームページ
  • URL: http://www.yakusaku.jp/