CRISPR-mediated reading and writing of the epigenome
| Project/Area Number |
26250038
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Kyushu University |
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Principal Investigator |
Ito Takashi 九州大学, 医学研究院, 教授 (90201326)
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| Research Collaborator |
MIURA Fumihito 九州大学, 大学院医学研究院, 講師 (50447348)
OKADA Satoshi 九州大学, 大学院医学研究院, 助教 (30734488)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥30,160,000 (Direct Cost: ¥23,200,000、Indirect Cost: ¥6,960,000)
Fiscal Year 2016: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2015: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2014: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
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| Keywords | エピゲノム / ゲノム編集 / 次世代シーケンシング / CRISPR/Cas9 / dCas9 / DNAメチル化 / BiFC / エピジェネティクス / CRISPR / PBAT |
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| Outline of Final Research Achievements |
The cell responds to environmental changes by altering its genome expression pattern, but not the genome sequences per se, and inherits the pattern even after cell division. This mechanism is called epigenetics, which is based on various chemical modifications of DNA and proteins comprising the chromosomes, and the term epigenome indicates the genome-wide pattern of these epigenetic modifications. It becomes increasingly possible to read, write, and visualize the epigenetics of targeted genomic regions by combining the technologies of CRISPR/Cas9-based genome editing and the next-generation DNA sequencing. In this study, we have developed basic methods for these novel approaches in epigenomics.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] Software updates in the Illumina HiSeq platform affect whole-genome bisulfite sequencing2017
Author(s)
Toh, H., Shirane, K. (co-first author), Miura, F., Kubo, N., Ichiyanagi, K., Hayashi, K., Saitou, M., Suyama, M., Ito, T. & Sasaki, H
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Journal Title
BMC Genomics
Volume: 18
Issue: 1
Pages: 31-38
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] DNA Methylome Analysis Identifies Transcription Factor-Based Epigenomic Signatures of Multilineage Competence in Neural Stem/Progenitor Cells.2017
Author(s)
Sanosaka T, Imamura T, Hamazaki N, Chai M, Igarashi K, Ideta-Otsuka M, Miura F, Ito T, Fujii N, Ikeo K, Nakashima K
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Journal Title
Cell Rep
Volume: 20
Issue: 12
Pages: 2992-3003
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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