| Budget Amount *help |
¥40,300,000 (Direct Cost: ¥31,000,000、Indirect Cost: ¥9,300,000)
Fiscal Year 2016: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2015: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2014: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
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| Outline of Final Research Achievements |
The Moloney murine leukemia virus (MLV)-based retroviral vector MSCV-GFP, which is repressed by the histone H3 lysine 9 methyltranferase SETDB1 pathway in mESCs was used as a reporter provirus, we performed a genome-wide CRISPR screen to advance our understanding of retroelement silencing in mESCs. We identified more than 80 genes involved in this process including not only already known retroelement silencing genes but also multiple novel ones. Among the characterized novel factors, we find that they seem to work for silencing in different layers, thus upstream and parallel or downstream of SETDB1.
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