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Chromatin dynamics induced by inflammatory stimulation

Research Project

Project/Area Number 26250041
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field System genome science
Research InstitutionThe University of Tokyo

Principal Investigator

Wada Youichiro  東京大学, アイソトープ総合センター, 教授 (10322033)

Co-Investigator(Kenkyū-buntansha) 神吉 康晴  東京大学, アイソトープ総合センター, 助教 (00534869)
藤井 穂高  大阪大学, 微生物病研究所, 准教授 (30302665)
興梠 貴英  自治医科大学, 医学部, 准教授 (40401046)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2014: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
Keywords転写 / RNAポリメレースII / 炎症 / 内皮細胞 / ゲノム解析技術 / クロマチン / 質量分析
Outline of Final Research Achievements

Among vascular diseases, atherosclerosis is one of the most important factors of ischemic heart diseases and cerebrovascular diseases, and elucidation of the mechanism in consistent gene expression regulation of atherogenic proteins. To dissect the adhesion molecule gene regulation, we adopted a model using endothelial cells stimulated inflammatory stimulator, and presence of transcription complexes, which regulate multiple genes at the same time, was suggested. In this research, we performed (i) comprehensive chromatin interaction analysis, (ii) identification of interaction associated proteincomplex, (iii) intervention of chromatin structure, and it was observed that transcription factories regulated both induced and reduced genes from different chromosomes.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (17 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (6 results) Journal Article (7 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (4 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results)

  • [Int'l Joint Research] Oxford University(英国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Connecticut/Harvard University(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Shandong Univeristy/Huazhong Agricultural University(China)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Oxford University(英国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] University of Connecticut/Harvard University(米国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] Shandong University(China)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Dynamically and epigenetically coordinated GATA/ETS/SOX transcription is indispensable for endothelial cell differentiation2017

    • Author(s)
      Kanki Y, Nakaki R, Shimamura T, Matsunaga T, Yamamizu K, Katayama S, Suehiro JI, Osawa T, Aburatani H, Kodama T, Wada Y, Yamashita JK, Minami T.
    • Journal Title

      Nucleic Acids Research

      Volume: 印刷中 Issue: 8 Pages: 4344-4358

    • DOI

      10.1093/nar/gkx159

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The Role of Cdkn1A-Interacting Zinc Finger Protein 1 (CIZ1) in DNA Replication and Pathophysiology.2016

    • Author(s)
      Liu Q, Niu N, Wada Y, Liu J.
    • Journal Title

      Int J Mol Sci.

      Volume: 17 Issue: 2 Pages: 212-212

    • DOI

      10.3390/ijms17020212

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of Chronic Low-Dose Radiation on Human Neural Progenitor Cells.2016

    • Author(s)
      Katsura M, Cyou-Nakamine H, Zen Q, Zen Y, Nansai H, Amagasa S, Kanki Y, Inoue T, Kaneki K, Taguchi A, Kobayashi M, Kaji T, Kodama T, Miyagawa K, Wada Y, Akimitsu N, Sone H.
    • Journal Title

      Sci Rep.

      Volume: 6 Issue: 1 Pages: 20027-20027

    • DOI

      10.1038/srep20027

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Low-Dose Cadmium Upregulates VEGF Expression in Lung Adenocarcinoma Cells.2015

    • Author(s)
      Liu F, Wang B, Li L, Dong F, Chen X, Li Y, Dong X, Wada Y, Kapron CM, Liu J.
    • Journal Title

      Int J Environ Res Public Health

      Volume: 12 Issue: 9 Pages: 10508-21

    • DOI

      10.3390/ijerph120910508

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Distinct effects of Hoxa2 overexpression in cranial neural crest populations reveal that the mammalian hyomandibular-ceratohyal boundary maps within the styloid process.2015

    • Author(s)
      Kitazawa T, Fujisawa K, Narboux-Neme N, Arima Y, Kawamura Y, Inoue T, Wada Y, Kohro T, Aburatani H, Kodama T, Kim KS, Sato T, Uchijima Y, Maeda K, Miyagawa-Tomita S, Minoux M, Rijli FM, Levi G, Kurihara Y, Kurihara H
    • Journal Title

      Developmental Biology

      Volume: 15 Issue: 2 Pages: 162-174

    • DOI

      10.1016/j.ydbio.2015.04.007

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements.2014

    • Author(s)
      Inoue T, Kohro T, Tanaka T, Kanki Y, Li G, Poh HM, Mimura I, Kobayashi M, Taguchi A, Maejima T, Suehiro JI, Sugiyama A, Kaneki K, Aruga H, Dong S, Stevens JF, Yamamoto S, Tsutsumi S, Fujita T, Ruan X, Aburatani H, Nangaku M, Ruan Y, Kodama T, Wada Y.
    • Journal Title

      Genome Biology

      Volume: 15 Issue: 4

    • DOI

      10.1186/gb-2014-15-4-r63

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Direct Evidence for Pitavastatin Induced Chromatin Structure Change in the KLF4 Gene in Endothelial Cells.2014

    • Author(s)
      Maejima T, Inoue T, Kanki Y, Kohro T, Li G, Ohta Y, Kimura H, Kobayashi M, Taguchi A, Tsutsumi S, Iwanari H, Yamamoto S, Aruga H, Dong S, Stevens JF, Poh HM, Yamamoto K, Kawamura T, Mimura I,
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 5 Pages: e96005-e96005

    • DOI

      10.1371/journal.pone.0096005

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Dynamics of chromatin structure in stimulated vascular endothelial cells2016

    • Author(s)
      和田洋一郎
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば会議場(茨城県つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Dynamic chromatin movement in stimulated endothelial cells suggested by interactome analysis2016

    • Author(s)
      和田洋一郎
    • Organizer
      3D nucleome workshop
    • Place of Presentation
      Huazhong Agricultural University, Wuhan, China
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 炎症刺激におけるクロマチン構造変化を介した 遺伝子発現の制御機構2016

    • Author(s)
      和田洋一郎
    • Organizer
      第17回日本分子脳神経外科学会
    • Place of Presentation
      帝京大学板橋キャンパス本部棟(東京都板橋区)
    • Year and Date
      2016-08-26
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Dynamic chromatin movement in stimulated endothelial cells suggested by interactome analysis2015

    • Author(s)
      和田洋一郎
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2015-12-02
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2014-04-04   Modified: 2022-02-07  

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