Chromatin dynamics induced by inflammatory stimulation
Project/Area Number |
26250041
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
System genome science
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Research Institution | The University of Tokyo |
Principal Investigator |
Wada Youichiro 東京大学, アイソトープ総合センター, 教授 (10322033)
|
Co-Investigator(Kenkyū-buntansha) |
神吉 康晴 東京大学, アイソトープ総合センター, 助教 (00534869)
藤井 穂高 大阪大学, 微生物病研究所, 准教授 (30302665)
興梠 貴英 自治医科大学, 医学部, 准教授 (40401046)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2014: ¥21,320,000 (Direct Cost: ¥16,400,000、Indirect Cost: ¥4,920,000)
|
Keywords | 転写 / RNAポリメレースII / 炎症 / 内皮細胞 / ゲノム解析技術 / クロマチン / 質量分析 |
Outline of Final Research Achievements |
Among vascular diseases, atherosclerosis is one of the most important factors of ischemic heart diseases and cerebrovascular diseases, and elucidation of the mechanism in consistent gene expression regulation of atherogenic proteins. To dissect the adhesion molecule gene regulation, we adopted a model using endothelial cells stimulated inflammatory stimulator, and presence of transcription complexes, which regulate multiple genes at the same time, was suggested. In this research, we performed (i) comprehensive chromatin interaction analysis, (ii) identification of interaction associated proteincomplex, (iii) intervention of chromatin structure, and it was observed that transcription factories regulated both induced and reduced genes from different chromosomes.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Dynamically and epigenetically coordinated GATA/ETS/SOX transcription is indispensable for endothelial cell differentiation2017
Author(s)
Kanki Y, Nakaki R, Shimamura T, Matsunaga T, Yamamizu K, Katayama S, Suehiro JI, Osawa T, Aburatani H, Kodama T, Wada Y, Yamashita JK, Minami T.
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Journal Title
Nucleic Acids Research
Volume: 印刷中
Issue: 8
Pages: 4344-4358
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Effects of Chronic Low-Dose Radiation on Human Neural Progenitor Cells.2016
Author(s)
Katsura M, Cyou-Nakamine H, Zen Q, Zen Y, Nansai H, Amagasa S, Kanki Y, Inoue T, Kaneki K, Taguchi A, Kobayashi M, Kaji T, Kodama T, Miyagawa K, Wada Y, Akimitsu N, Sone H.
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Journal Title
Sci Rep.
Volume: 6
Issue: 1
Pages: 20027-20027
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Distinct effects of Hoxa2 overexpression in cranial neural crest populations reveal that the mammalian hyomandibular-ceratohyal boundary maps within the styloid process.2015
Author(s)
Kitazawa T, Fujisawa K, Narboux-Neme N, Arima Y, Kawamura Y, Inoue T, Wada Y, Kohro T, Aburatani H, Kodama T, Kim KS, Sato T, Uchijima Y, Maeda K, Miyagawa-Tomita S, Minoux M, Rijli FM, Levi G, Kurihara Y, Kurihara H
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Journal Title
Developmental Biology
Volume: 15
Issue: 2
Pages: 162-174
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements.2014
Author(s)
Inoue T, Kohro T, Tanaka T, Kanki Y, Li G, Poh HM, Mimura I, Kobayashi M, Taguchi A, Maejima T, Suehiro JI, Sugiyama A, Kaneki K, Aruga H, Dong S, Stevens JF, Yamamoto S, Tsutsumi S, Fujita T, Ruan X, Aburatani H, Nangaku M, Ruan Y, Kodama T, Wada Y.
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Journal Title
Genome Biology
Volume: 15
Issue: 4
Pages: R63-R63
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Direct Evidence for Pitavastatin Induced Chromatin Structure Change in the KLF4 Gene in Endothelial Cells.2014
Author(s)
Maejima T, Inoue T, Kanki Y, Kohro T, Li G, Ohta Y, Kimura H, Kobayashi M, Taguchi A, Tsutsumi S, Iwanari H, Yamamoto S, Aruga H, Dong S, Stevens JF, Poh HM, Yamamoto K, Kawamura T, Mimura I,
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Journal Title
PLoS One.
Volume: 9
Issue: 5
Pages: e96005-e96005
DOI
Related Report
Peer Reviewed / Open Access
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