Mechanism and Evolution of RNA polymerases
| Project/Area Number |
26251009
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | The University of Tokyo (2016)
National Institute of Advanced Industrial Science and Technology (2014-2015) |
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Principal Investigator |
Tomita Kozo 東京大学, 大学院新領域創成科学研究科, 教授 (00345274)
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| Project Period (FY) |
2014-06-27 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥40,560,000 (Direct Cost: ¥31,200,000、Indirect Cost: ¥9,360,000)
Fiscal Year 2016: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2015: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
Fiscal Year 2014: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
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| Keywords | RNA / 構造 / 反応機構 / 反応分子機構 / ヌクレオチド転移酵素 / X線結晶構造 / 酵素反応 / 構造解析 / 機能解析 / RNA合成 / X線結晶 / tRNA / 蛋白質 |
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| Outline of Final Research Achievements |
In this project, we focus on the molecular mechanism of RNA polymerizations by CC-, A- CCA-adding enzymes and by RNA polymerase complexed with S1 protein. We clarified the mechanisms of CC-addition and A-addition by CC-adding and A-adding enzymes, respectively. We also clarify the mechanism by which CCA-adding enzyme discriminates mature tRNA from immature tRNA. We also clarified the molecular basis of replication initiation by using S1 protein in RNA polymerase complex.
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Report
(4 results)
Research Products
(28 results)
