• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Roles and modes of action of nectins in heterotypic cell-cell adhesions

Research Project

Project/Area Number 26251013
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionKobe University

Principal Investigator

Takai Yoshimi  神戸大学, 医学研究科, 特命教授 (60093514)

Co-Investigator(Kenkyū-buntansha) 下野 洋平  神戸大学, 医学(系)研究科(研究院), 准教授 (90594630)
水谷 清人  神戸大学, 医学(系)研究科(研究院), 講師 (50559177)
宮田 宗明  神戸大学, 医学(系)研究科(研究院), 助教 (90582007)
溝口 明  三重大学, 医学(系)研究科(研究院), 教授 (90181916)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥40,820,000 (Direct Cost: ¥31,400,000、Indirect Cost: ¥9,420,000)
Fiscal Year 2016: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2015: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2014: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Keywords細胞接着・運動 / 細胞シグナル伝達機構 / 異種細胞間接着 / 細胞膜受容体 / ネクチン / アファディン / 細胞情報伝達機構
Outline of Final Research Achievements

The roles and modes of action of nectins in heterotypic cell-cell adhesions were investigated. We identified a novel cell adhesion apparatus formed by the heterophilic trans-interaction between nectin-4 and nectin-1, which were expressed in the luminal and basal cells in the mammary gland, respectively. Nectin-4 in this apparatus enhanced the prolactin receptor signaling. Moreover, we showed that nectin-2 and nectin-3 expressed in olfactory and supporting cells, respectively, interacted in trans and recruited cadherin to the heterotypic cell junctions, resulting in the formation of the mosaic pattern in the olfactory epithelium. Further, we showed that nectin-2δ was localized on the plasma membranes of astrocytic perivascular endfoot processes facing the basement membrane of blood vessels in the brain. Genetic ablation of nectin-2 caused degeneration of the astrocytic perivascular endfoot processes and neurons, indicating that nectin- 2 is required to maintain the brain structures.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (40 results)

All 2017 2016 2015 2014

All Journal Article (27 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 27 results,  Open Access: 15 results,  Acknowledgement Compliant: 10 results) Presentation (12 results) (of which Invited: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Nectin-4 co-stimulates the prolactin receptor by interacting with SOCS1 and inhibiting its activity on the JAK2-STAT5a signaling pathway.2017

    • Author(s)
      Maruoka, M., Kedashiro, S., Ueda, Y., Mizutani, K., and Takai, Y.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 292 Issue: 17 Pages: 6895-6909

    • DOI

      10.1074/jbc.m116.769091

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Roles of afadin in the formation of the cellular architecture of the mouse hippocampus and dentate gyrus.2017

    • Author(s)
      Miyata M, Maruo T, Kaito A, Wang S, Yamamoto H, Fujiwara T, Mizoguchi A, Mandai K, Takai Y
    • Journal Title

      Mol Cell Neurosci

      Volume: 79 Pages: 34-44

    • DOI

      10.1016/j.mcn.2016.12.007

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aging-dependent expression of synapse-related proteins in the mouse brain.2017

    • Author(s)
      Shiotani, H., Maruo, T., Sakakibara, S., Miyata, M., Mandai, K., Mochizuki, H., Takai, Y.
    • Journal Title

      Genes to Cells

      Volume: 印刷中 Issue: 5 Pages: 472-484

    • DOI

      10.1111/gtc.12489

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Multiple roles of afadin in the ultrastructural morphogenesis of mouse hippocampal mossy fiber synapses.2017

    • Author(s)
      Sai, K., Wang, S., Kaito, A., Fujiwara, T., Maruo, T., Itoh, Y., Miyata, M., Sakakibara, S., Miyazaki, N., Murata, K., Yamaguchi, Y., Haruta, T., Nishioka, H., Motojima, Y., Komura, M., Kimura, K., Mandai, K., Takai, Y., and Mizoguchi, A.
    • Journal Title

      J Comp Neurol

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus.2016

    • Author(s)
      Kobayashi S, Hida Y, Ishizaki H, Inoue E, Tanaka-Okamoto M, Yamasaki M, Miyazaki T, Masahiro F, Kitajima I, Takai Y, Watanabe M, Ohtsuka T, Manabe T
    • Journal Title

      Eur J Neurosci.

      Volume: 44 Issue: 5 Pages: 2272-2284

    • DOI

      10.1111/ejn.13331

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Localization of nectin-2δ at perivascular astrocytic endfoot processes and degeneration of astrocytes and neurons in nectin-2 knockout mouse brain.2016

    • Author(s)
      Miyata M, Mandai K, Maruo T, Sato J, Shiotani H, Kaito A, Itoh Y, Wang S, Fujiwara T, Mizoguchi A, Takai Y, Rikitake Y
    • Journal Title

      Brain Res

      Volume: 1649 Pages: 90-101

    • DOI

      10.1016/j.brainres.2016.08.023

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nectin spot: a novel type of nectin-mediated cell adhesion apparatus.2016

    • Author(s)
      Mizutani, K., and Takai, Y.
    • Journal Title

      Biochemical Journal

      Volume: 473 Issue: 18 Pages: 2691-2715

    • DOI

      10.1042/bcj20160235

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Regulatory role of the cell adhesion molecule nectin-1 in GABAergic inhibitory synaptic transmission in the CA3 region of mouse hippocampus.2016

    • Author(s)
      Geng, X., Mandai, K., Maruo, T., Wang, S., Fujiwara, T., Mizoguchi, A., Takai, Y., and Mori, M.
    • Journal Title

      Genes Cells

      Volume: 21 Issue: 1 Pages: 88-98

    • DOI

      10.1111/gtc.12322

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Synergistic action of nectins and cadherins generates the mosaic cellular pattern of the olfactory epithelium.2016

    • Author(s)
      Sayaka Katsunuma, Hisao Honda, Tomoyasu Shinoda, Yukitaka Ishimoto, Takaki Miyata, Hiroshi Kiyonari, Takaya Abe, Ken-ichi Nibu, Yoshimi Takai, and Hideru Togashi.
    • Journal Title

      The Journal of Cell Biology

      Volume: 212 Issue: 5 Pages: 561-75

    • DOI

      10.1083/jcb.201509020

    • NAID

      120005766825

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] A Novel Nectin-mediated Cell Adhesion Apparatus Which Is Implicated in Prolactin Receptor Signaling for Mammary Gland Development2016

    • Author(s)
      Kitayama M, Mizutani K, Maruoka M, Mandai K, Sakakibara S, Ueda Y, Komori T, Shimono Y, Takai Y
    • Journal Title

      Journal of Biological Chemistry

      Volume: 291 Issue: 11 Pages: 5817-5831

    • DOI

      10.1074/jbc.m115.685917

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Activity-dependent alteration of the morphology of a hippocampal giant synapse.2016

    • Author(s)
      Maruo, T., Mandai, K., Takai, Y., and Mori, M.
    • Journal Title

      Mol Cell Neurosci

      Volume: 71 Pages: 25-33

    • DOI

      10.1016/j.mcn.2015.12.005

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nectin-1 spots regulate the branching of olfactory mitral cell dendrites.2015

    • Author(s)
      Fujiwara, T., Inoue, T., Maruo, T., Rikitake, Y., Ieki, N., Mandai, K., Kimura, K., Kayahara, T., Wang, S., Itoh, Y., Sai, K., Mori, M., Mori, K., Takai, Y., and Mizoguchi, A.
    • Journal Title

      Mol Cell Neurosci

      Volume: 68 Pages: 143-150

    • DOI

      10.1016/j.mcn.2015.07.003

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nectin-1 spots as a novel adhesion apparatus that tethers mitral cell lateral dendrites in a dendritic meshwork structure of the developing mouse olfactory bulb.2015

    • Author(s)
      Inoue, T., Fujiwara, T., Rikitake, Y., Maruo, T., Mandai, K., Kimura, K, Kayahara, T, Wang, S., Itoh, Y., Sai, K., Mori, M., Mori, K., Mizoguchi, A., and Takai, Y.
    • Journal Title

      J Comp Neurol

      Volume: 523 Issue: 12 Pages: 1824-1839

    • DOI

      10.1002/cne.23762

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impairment of radial glial scaffold-dependent neuronal migration and formation of double cortex by genetic ablation of afadin.2015

    • Author(s)
      Yamamoto H, Mandai K, Konno D, Maruo T, Matsuzaki F, Takai Y
    • Journal Title

      Brain Res

      Volume: 43 Pages: 775-86

    • DOI

      10.1016/j.brainres.2015.05.012

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of CXCR4 in Metastasized Breast Cancer Cells and Implication in Their Dormancy2015

    • Author(s)
      Nobutani K, Shimono Y, Mizutani K, Ueda Y, Suzuki T, Kitayama M, Minami A, Momose K, Miyawaki K, Akashi K, Azuma T, Takai Y
    • Journal Title

      PLoS One

      Volume: 10 Issue: 6 Pages: e0130032-e0130032

    • DOI

      10.1371/journal.pone.0130032

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The cell adhesion molecule necl-4/cadm4 serves as a nobel regulator for contact inhibition of cell movement and proliferation.2015

    • Author(s)
      Yamana S, Tokiyama A, Mizutani K, Hirata K, Takai Y, Rikitake Y.
    • Journal Title

      PloS One.

      Volume: 10 Issue: 4 Pages: e0124259-e0124259

    • DOI

      10.1371/journal.pone.0124259

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nectin-1 spots as a novel adhesion apparatus that tethers mitral cell lateral dendrites in a dendritic meshwork structure of the developing mouse olfactory bulb.2015

    • Author(s)
      Inoue T, Fujiwara T, Rikitake Y, Maruo T, Mandai K, Kimura K, Kayahara T, Wang S, Ioth Y, Sai K, Mori M, Mori K, Mizoguchi A, Takai Y.
    • Journal Title

      J Comp Neurol.

      Volume: 印刷中

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Human T-cell leukemia virus type 1 (HTLV-1) tax requires CADM1/TSLC1 for inactivation of the NF-κB inhibitor A20 and constitutive NF-κB signaling.2015

    • Author(s)
      Pujari R, Hunte R, Thomas R, van der Weyden L, Rauch D, Ratner L, Nyborg JK, Ramos JC, Takai Y, Shembade N.
    • Journal Title

      PLoS Pathog.

      Volume: 16 Issue: 3 Pages: e1004721-e1004721

    • DOI

      10.1371/journal.ppat.1004721

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nectins and nectin-like molecules in development and disease.2015

    • Author(s)
      Mandai K, Rikitake Y, Mori M, Takai Y.
    • Journal Title

      Curr Top Dev Biol.

      Volume: 112 Pages: 197-231

    • DOI

      10.1016/bs.ctdb.2014.11.019

    • ISBN
      9780124077584
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Crystal structure of afadin PDZ domain-nectin-3 complex shows the structural plasticity of the ligand-binding site.2015

    • Author(s)
      Fujiwara Y, Goda N, Tamashiro T, Narita H, Satomura K, Tenno T, Nakagawa A, Oda M, Suzuki M, Sakisaka T, Takai Y, Hiroaki H.
    • Journal Title

      Protein Sci.

      Volume: 24 Issue: 3 Pages: 376-385

    • DOI

      10.1002/pro.2628

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Quantitative analysis of the cellular composition in seminiferous tubules in normal and genetically modified infertile mice.2015

    • Author(s)
      Nakata H, Wakayama T, Takai Y, Iseki S.
    • Journal Title

      J Histochem Cytochem.

      Volume: 63 Issue: 2 Pages: 99-113

    • DOI

      10.1369/0022155414562045

    • NAID

      120005593935

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic ablation of afadin causes mislocalization and deformation of Paneth cells in the mouse small intestinal epithelium.2014

    • Author(s)
      Tanaka-Okamoto M, Itoh Y, Miyoshi J, Mizoguchi A, Mizutani K, Takai Y, Inoue M.
    • Journal Title

      PLoS One.

      Volume: 9 Issue: 10 Pages: e110549-e110549

    • DOI

      10.1371/journal.pone.0110549

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Afadin requirement for cytokine expressions in keratinocytes during chemically induced inflammation in mice.2014

    • Author(s)
      Yoshida T, Iwata T, Takai Y, Birchmeier W, Yamato M, Okano T.
    • Journal Title

      Genes Cells.

      Volume: 19 Issue: 11 Pages: 842-852

    • DOI

      10.1111/gtc.12184

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] s-Afadin binds more preferentially to the cell adhesion molecules nectins than l-afadin.2014

    • Author(s)
      Kobayashi R, Kurita S, Miyata M, Maruo T, Mandai K, Rikitake Y, Takai Y.
    • Journal Title

      Genes Cells.

      Volume: 19 Issue: 12 Pages: 853-863

    • DOI

      10.1111/gtc.12185

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Syntaxin-binding protein STXBP5 inhibits endothelial exocytosis and promotes platelet secretion.2014

    • Author(s)
      Zhu Q, Yamakuchi M, Ture S, de la Luz Garcia-Hernandez M, Ko KA, Modjeski KL, LoMonaco MB, Johnson AD, O'Donnell CJ, Takai Y, Morrell CN, Lowenstein CJ.
    • Journal Title

      J Clin Invest.

      Volume: 124 Issue: 10 Pages: 4503-4516

    • DOI

      10.1172/jci71245

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Platelet secretion and hemostasis require syntaxin-binding protein STXBP5.2014

    • Author(s)
      Ye S, Huang Y, Joshi S, Zhang J, Yang F, Zhang G, Smyth SS, Li Z, Takai Y, Whiteheart SW.
    • Journal Title

      J Clin Invest.

      Volume: 124 Issue: 10 Pages: 4517-4528

    • DOI

      10.1172/jci75572

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Suppression of the TGF-β1-induced protein expression of SNAI1 and N-cadherin by miR-199a.2014

    • Author(s)
      Suzuki T, Mizutani K, Minami A, Nobutani K, Kurita S, Nagino M, Shimono Y, Takai Y.
    • Journal Title

      Genes Cells.

      Volume: 19 Issue: 9 Pages: 667-675

    • DOI

      10.1111/gtc.12166

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Roles of afadin in the development of the cellular architecture of the hippocampus.2016

    • Author(s)
      宮田宗明、丸尾知彦、山本昆明、垣内愛加、王淑杰、藤原武志、溝口明、萬代研二、高井義美
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] ネクチン-2αとマウス手綱核2016

    • Author(s)
      塩谷元、萬代研二、宮田宗明、丸尾知彦、垣内愛加、王淑杰、藤原武志、溝口明、望月秀樹、高井義美
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] 海馬苔状線維シナプス分化における2つのアファディンスプライスバリアントの役割2016

    • Author(s)
      丸尾知彦、萬代研二、榊原正太郎、伊藤優、藤原武志、王淑杰、崔煌植、垣内愛加、耿瀟奇、森正弘
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] 細胞の接着とシグナル伝達2015

    • Author(s)
      高井義美
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会 (BMB2015)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 乳腺分化を制御する新規のネクチン依存性細胞間接着装置2015

    • Author(s)
      圓岡真宏、北山-吉田美登里、水谷清人、高井義美
    • Organizer
      第38回日本分子生物学会年会・第88回日本生化学会大会 合同大会  (BMB2015)
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Annual Research Report
  • [Presentation] 転移乳がん細胞におけるCXCR4の発現低下とがん休眠との関連2015

    • Author(s)
      上田悠貴、信谷健太郎、下野洋平、水谷清人、鈴木俊裕、北山美登里、南晶洋、百瀬健次、宮脇恒太、赤司浩一、東健、高井義美
    • Organizer
      第74回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] AdAMS: Activith-dependent alteration of morphology of a hippocampal mossy fiber bouton.2015

    • Author(s)
      Maruo Tomohiko, Mandai Kenji, Takai Yoshimi, Mori Masahiro
    • Organizer
      第38回日本神経科学大会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2015-07-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] SBF-SEM 3D reconstitution analysis reveals alterations in composition and morphology of mouse hippocampal mossy fiber synapses by afadin knockout2015

    • Author(s)
      Takeshi Fujiwara, Shujie Wang, Yu Itoh, Kousyoku Sai, Aika Kaito, Naoyuki Miyazaki, Kazuyoshi Murata, Tomohiko Maruo, Hideaki Yamamoto, Kenji Mandai, Yoshimi Takai, Akira Mizoguchi
    • Organizer
      第120回日本解剖学会総会・全国学術集会・第92回日本生理学会大会合同大会
    • Place of Presentation
      神戸国際会議場・展示場
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Afadin plays an important role in formation of puncta adherentia junction and differentiation of presynapses in hippocampal neurons.2014

    • Author(s)
      Daisaku Toyoshima, Kenji Mandai, Tomohiko Maruo, Irwan Supriyanto, Hideru Togashi, Takahito Inoue, Masahiro Mori, Yoshimi Takai
    • Organizer
      Neuroscience 2014
    • Place of Presentation
      Washington, DC, USA
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] マイクロRNA-199aはTGFβ-1で誘導されるSNAI1とN-cadherinの発現を 抑制する2014

    • Author(s)
      鈴木俊裕、水谷清人、南晶洋、信谷健太郎、梛野正人、下野洋平、 高井義美
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Afadin-deficiency leads to the formation of double cortex by abnormal radial glial scaffold and cell-autonomous impairment of neuronal migration during development.2014

    • Author(s)
      Hideaki Yamamoto, Kenji Mandai, Daijiro Konno, Tomohiko Maruo, Fumio Matsuzaki, Yoshimi Takai
    • Organizer
      2014 CSHL Conference on Glia in Health & Disease
    • Place of Presentation
      New York, NY, USA
    • Year and Date
      2014-07-17 – 2014-07-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] シグナル伝達と細胞接着―研究の歴史と展望―2014

    • Author(s)
      高井義美
    • Organizer
      第100回日本消化器病学会総会
    • Place of Presentation
      東京国際フォーラム
    • Year and Date
      2014-04-23 – 2014-04-26
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Patent(Industrial Property Rights)] ErbB3活性化に伴うシグナルの伝達抑制物質及びそのスクリーニング方法2016

    • Inventor(s)
      高井義美、水谷清人、慶田城迅
    • Industrial Property Rights Holder
      高井義美、水谷清人、慶田城迅
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2016-096427
    • Filing Date
      2016-05-12
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi