Innovation of chemiluminogenetics capable of noninvasive manipulation of biological functions deep inside body
| Project/Area Number |
26251018
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
Nagai Takeharu 大阪大学, 産業科学研究所, 教授 (20311350)
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| Co-Investigator(Renkei-kenkyūsha) |
HIGUCHI YURIKO 京都大学, 健康長寿社会の総合医療開発ユニット, 講師 (40402797)
SUGA HIROAKI 東京大学, 理学(系)研究科(研究院), 教授 (00361668)
ENOKI RYOSUKE 北海道大学, 医学(系)研究科(研究院), 助教 (00528341)
INOUE KEN-ICHI 京都大学, 霊長類研究所, 助教 (90455395)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2015: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2014: ¥22,880,000 (Direct Cost: ¥17,600,000、Indirect Cost: ¥5,280,000)
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| Keywords | 発光タンパク質 / 光感受性タンパク質 / オプトジェネティクス / 発光基質 / ケミルミノジェネティクス / 化学発光タンパク質 |
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| Outline of Final Research Achievements |
In this study, we developed several fundamental technologies for
“Chemiluminogenetics” which enables noninvasive manipulation of physiological functions in deep tissue or body by using excited state energy of luminescence reaction in chemiluminescent proteins upon substrate addition. First, we fused an Oplopholus luciferase with a green fluorescent protein, yielding a bright chemiluminescent protein, GeNL that has more than 10-fold brightness compared with a previously developed chemiluminescent protein, Nano-lantern. We also developed a red-shifted version, ReNL which can be applied for low illumination light therapy in deep tissue. Furthermore, we developed a genetically-encoded photosensitizer Green SuperNova that can produce more reactive oxygen species than previous photosensitizer.
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Report
(4 results)
Research Products
(77 results)
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[Journal Article] A Temporary Gating of Actin Remodeling during Synaptic Plasticity Consists of the Interplay between the Kinase and Structural Functions of CaMKII.2015
Author(s)
Kim, K., Lakhanpal, G., Lu, HE., Khan, M., Suzuki, A., Kato-Hayashi, M., Narayanan, R., Luyben, TT., Matsuda, T., Nagai, T., Blanpied, TA., Hayashi, Y. and Okamoto K.
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Journal Title
Neuron
Volume: 87
Issue: 4
Pages: 813-826
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Super-duper chemilumiescent proteins2016
Author(s)
Takeharu Nagai
Organizer
University of Bordeaux-Osaka,University, Osaka City University, Mini-Symposium,on Synthetic
Place of Presentation
ISIR, Osaka University (Ibaraki, Osaka, Japan)
Year and Date
2016-01-26
Related Report
Int'l Joint Research / Invited
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[Presentation] 生物発光が拓く未来社会2015
Author(s)
永井 健治
Organizer
第53回日本生物物理学会年会・市民講演会
Place of Presentation
石川県教育会館 ホール (石川県金沢市)
Year and Date
2015-09-12
Related Report
Invited
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[Presentation] 平成26 年度派遣報告2015
Author(s)
稲垣 成矩
Organizer
第4回産研若手人材育成派遣ワークショップ
Place of Presentation
大阪大学産業科学研究所 (大阪府茨木市)
Year and Date
2015-07-24
Related Report
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