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Gene regulatory network underlying epiblast cell determination and their derivation into various somatic cell lineages

Research Project

Project/Area Number 26251024
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionKyoto Sangyo University

Principal Investigator

KONDOH Hisato  京都産業大学, 総合生命科学部, 教授 (70127083)

Co-Investigator(Renkei-kenkyūsha) ISHII Yasuo  東京女子医科大学, 医学部, 講師 (20582430)
TAKEMOTO Tatsuya  徳島大学, 先端酵素学研究所, 教授 (30443899)
ARUGA Jun  長崎大学, 医歯薬学総合研究科, 教授 (10232076)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥40,430,000 (Direct Cost: ¥31,100,000、Indirect Cost: ¥9,330,000)
Fiscal Year 2016: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2015: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2014: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Keywords遺伝子発現調節 / 転写因子 / エピブラスト / 幹細胞 / 体細胞系列 / 胚発生 / ノード / エピブラスト幹細胞 / マウス胚操作
Outline of Final Research Achievements

Diverse somatic cells develop from pluripotent epiblast cells that are established after embryo implantation. However, the gene regulatory network underlying epiblast cell state determination and that involved in somatic cell derivation remains unclear. We investigated the regulatory function of major transcription factors (TFs) in the epiblast, and showed a drastic change in the gene regulatory network in the epiblast compared with the preimplantation pluripotent state. To characterize the process of somatic cell derivation, we established stem cell lines that represent early somatic lineages, e.g., neural progenitor cell lines with anteroposterior regional specificities, and characterized these intermediate somatic cell states. In addition, we investigated the regulatory function of the TF SOX2 in determining the regional specificity of endodermal tissues.

Report

(4 results)
  • 2017 Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (54 results)

All 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (4 results) Journal Article (12 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 12 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (30 results) (of which Int'l Joint Research: 15 results,  Invited: 8 results) Book (1 results) Remarks (7 results)

  • [Int'l Joint Research] Emory University of Medicine/Albert Einstein College of Medicine(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Tel-Aviv University/Hebrew University of Jerusalem(Israel)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Katholieke Universiteit Leuven(Belgium)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Erasmus University Medical Center(オランダ)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Roles of ZIC2 in Regulation of Pluripotent Stem Cells2018

    • Author(s)
      Kondoh Hisato
    • Journal Title

      Adv Exp Med Biol.

      Volume: 1046 Pages: 339-351

    • DOI

      10.1007/978-981-10-7311-3_17

    • ISBN
      9789811073106, 9789811073113
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nasal and otic placode specific regulation of Sox2 involves both activation by Sox-Sall4 synergism and multiple repression mechanisms2018

    • Author(s)
      Sugahara Satoko、Fujimoto Tooru、Kondoh Hisato、Uchikawa Masanori
    • Journal Title

      Developmental biology

      Volume: 433 Issue: 1 Pages: 61-74

    • DOI

      10.1016/j.ydbio.2017.11.005

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancer Analyses Using Chicken Embryo Electroporation2017

    • Author(s)
      Uchikawa Masanori、Nishimura Naoko、Iwafuchi-Doi Makiko、Kondoh Hisato
    • Journal Title

      Methods in molecular biology (Clifton, N.J.)

      Volume: 1650 Pages: 191-202

    • DOI

      10.1007/978-1-4939-7216-6_12

    • ISBN
      9781493972159, 9781493972166
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ChIP-seq analysis of genomic binding regions of five major transcription factors highlights a central role for ZIC2 in the mouse epiblast stem cell gene regulatory network2017

    • Author(s)
      Matsuda Kazunari、Mikami Tomoyuki、Oki Shinya、Iida Hideaki、Andrabi Munazah、Boss Jeremy M.、Yamaguchi Katsushi、Shigenobu Shuji、Kondoh Hisato
    • Journal Title

      Development

      Volume: 144 Pages: 1948-1958

    • DOI

      10.1242/dev.143479

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Intrinsic lens potential of neural retina inhibited by Notch signaling as the cause of lens transdifferentiation2017

    • Author(s)
      Iida Hideaki、Ishii Yasuo、Kondoh Hisato
    • Journal Title

      Dev. Bill.

      Volume: 421 Issue: 2 Pages: 118-125

    • DOI

      10.1016/j.ydbio.2016.11.004

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sip1 regulates the generation of the inner nuclear layer retinal cell lineages in mammals.2016

    • Author(s)
      Menuchin-Lasowski Y., Oren-Giladi P., Xie Q., Ezra-Elia R., Ofri R., Peled-Hajaj S., Farhy C., Higashi Y., Van de Putte T., Kondoh H., Huylebroeck D., Cvekl A., Ashery-Padan R.
    • Journal Title

      Development. 143:2829-2841 (2016)

      Volume: 143 Issue: 15 Pages: 829-2841

    • DOI

      10.1242/dev.136101

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Axial level-dependent molecular and cellular mechanisms underlying the genesis of the embryonic neural plate.2016

    • Author(s)
      Kondoh H, Takada S, Takemoto T.
    • Journal Title

      Development, Growth & Differentiation.

      Volume: 58 Issue: 5 Pages: 427-427

    • DOI

      10.1111/dgd.12295

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] R26-WntVis reporter mice showing graded response to Wnt signal levels.2016

    • Author(s)
      Takemoto T, Abe T, Kiyonari H, Nakao K, Furuta Y, Suzuki H, Takada S, Fujimori T, Kondoh H.
    • Journal Title

      Genes Cells.

      Volume: 印刷中 Issue: 6 Pages: 661-669

    • DOI

      10.1111/gtc.12364

    • NAID

      120006306641

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulation of trunk neural crest delamination by δEF1 and Sip1 in the chicken embryo.2016

    • Author(s)
      Yasumi T, Inoue M, Maruhashi M, Kamachi Y, Higashi Y, Kondoh H, Uchikawa M.
    • Journal Title

      Dev Growth Differ.

      Volume: 58 Pages: 205-214

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] YAP is essential for tissue tension to ensure vertebrate 3D body shape2015

    • Author(s)
      Sean Porazinski, Huijia Wang, Yoichi Asaoka, Martin Behrndt, Tatsuo Miyamoto, Hitoshi Morita, Shoji Hata et al.
    • Journal Title

      Nature

      Volume: 印刷中 Issue: 7551 Pages: 217-221

    • DOI

      10.1038/nature14215

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Transcriptional repressor ZEB2 promotes terminal differentiation of CD8+ effector and memory T cell populations during infection.2015

    • Author(s)
      Omilusik KD, Best JA, Yu B, Goossens S, Weidemann A, Nguyen JV, Seuntjens E, Stryjewska A, Zweier C, Roychoudhuri R, Gattinoni L, Bird LM, Higashi Y, Kondoh H, Huylebroeck D, Haigh J, Goldrath AW.
    • Journal Title

      J Exp Med.

      Volume: 12 Issue: 12 Pages: 2027-2039

    • DOI

      10.1084/jem.20150194

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Sixteen additional enhancers associated with the chicken Sox2 locus outside the central 50‐kb region2014

    • Author(s)
      Ryuji Okamoto, Masanori Uchikawa, Hisato Kondoh.
    • Journal Title

      Development, Growth & Differentiation

      Volume: 57 Issue: 1 Pages: 24-39

    • DOI

      10.1111/dgd.12185

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] SOX2-dependent determination of tissue identity in the foregut.2017

    • Author(s)
      Teramoto M, Sugawara R, Ishii Y, Kuroiwa A, Kondoh H.
    • Organizer
      ConBio2017
    • Related Report
      2016 Annual Research Report
  • [Presentation] A drastic change in the major regulatory TFs from SOX2-POU5F1 to ZIC2-OTX2 in the pluripotent stem cells representing pre-implantation and post-implantation stages.2017

    • Author(s)
      Kondoh H, Matsuda K, Oki S, Shigenobu S.
    • Organizer
      ConBio2017
    • Related Report
      2016 Annual Research Report
  • [Presentation] エピブラスト幹細胞株から樹立された領域特性をもつ神経幹細胞株の解析2017

    • Author(s)
      中村香絵、Boitet C、吉氷康矢、飯田英明、近藤寿人
    • Organizer
      ConBio2017
    • Related Report
      2016 Annual Research Report
  • [Presentation] Foxa2とともにnEGFPを発現するエピブラスト幹細胞を用いた、初期発生過程の研究2017

    • Author(s)
      稲森祥子、石井泰雄、近藤寿人
    • Organizer
      ConBio2017
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヘンゼン結節は脊索前板の発生を介して、Otx2を発現するエピブラストに頭部を形成させる2017

    • Author(s)
      吉氷康矢、近藤寿人
    • Organizer
      ConBio2017
    • Related Report
      2016 Annual Research Report
  • [Presentation] Sox2の神経系での発現を制御するD1エンハンサーの解析2017

    • Author(s)
      飯田英明、内川昌則、近藤寿人
    • Organizer
      ConBio2017
    • Related Report
      2016 Annual Research Report
  • [Presentation] SOX2-dependent determination of tissue identity in the foregut.2017

    • Author(s)
      Teramoto M, Sugawara R, Ishii Y, Kuroiwa A, Kondoh H.
    • Organizer
      ISDB 2017
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Roles for ZIC2 in the regulation of epiblast stem cells and embryonic stem cells.2017

    • Author(s)
      Kondoh H, Matsuda K, Oki S, Shigenobu S.
    • Organizer
      ISDB 2017
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Embryonic region-dependent development and interactions of grafted Hensen’s node with host tissues: reconsideration of organizer model.2017

    • Author(s)
      Kondoh H, Yoshihi K.
    • Organizer
      50th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Intrinsic lens potential of neural retina inhibited by Notch signaling as the cause of “lens transdifferentiation”.2017

    • Author(s)
      Iida H, Ishii Y, Kondoh H.
    • Organizer
      50th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Establishment of neural progenitor cells lines with defined regional specificities from EpiSCs.2017

    • Author(s)
      Nakamura K, Boitet C, Satake S, Yoshihi K, Iida H, Ishii Y, Kondoh H.
    • Organizer
      50th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Establishment and use of a new epiblast stem cell line marking Foxa2 expression with GFP.2017

    • Author(s)
      Inamori S, Ishii Y, Kondoh H.
    • Organizer
      50th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] SOX2-dependent determination of tissue identity in the foregut.2017

    • Author(s)
      Teramoto M, Sugawara R, Ishii Y, Kuroiwa A, Kondoh H.
    • Organizer
      50th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular and cellular mechanisms underlying lens transdifferentiation from embryonic neural retina.2016

    • Author(s)
      Kondoh H, Iida T
    • Organizer
      Avian Model Systems 9.
    • Place of Presentation
      Academia Sinica, Taipei, Taiwan
    • Year and Date
      2016-03-28
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional genomics of peri- and post-implantation stage stem cells2016

    • Author(s)
      Kondoh H, Matsuda K, Mikami, T. Oki S, Shigenobu S.
    • Organizer
      The 39th Annual Meeting of Molecular Biology Society Japan.
    • Related Report
      2016 Annual Research Report
  • [Presentation] Intrinsic lens potential of neural retina inhibited by Notch signaling as the cause of lens transdifferentiation.2016

    • Author(s)
      Iida T, Ishii Y, Kondoh H.
    • Organizer
      The 39th Annual Meeting of Molecular Biology Society Japan.
    • Related Report
      2016 Annual Research Report
  • [Presentation] Functional genomics of peri- and post-implantation stage stem cells.2016

    • Author(s)
      Kondoh, H.
    • Organizer
      IRM Distinguished Lectures, University of Pennsylvania Institute for Regenerative Medicine.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Genome-wide functional interactions of five major transcriptional factors in mouse epiblast stem cells.2016

    • Author(s)
      Kondoh H, Matsuda K, Mikami, T. Oki S, Shigenobu S.
    • Organizer
      Cell Symposia: Transcriptional Regulation in Development and Disease.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Simultaneous promotion of neuronal and lens development from chicken embryonic neural retina.2016

    • Author(s)
      Iida H, Ishii Y, Kondoh H.
    • Organizer
      49th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Reinvestigation of the developmental potentioal of grafted Hensen’s node.2016

    • Author(s)
      Yoshihi K, Ishii Y. Kondoh H.
    • Organizer
      49th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Direct derivation of embryonic neural progenitor cell lines from epiblast stem cells.2016

    • Author(s)
      Nakamura K, Boitet C, Satake S, Yoshihi K, Ishii Y, Kondoh H.
    • Organizer
      49th Annual Meeting of the Japanese society of Developmental Biologists.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regional specification of the neural primordia starting from the epiblast.2015

    • Author(s)
      H. Kondoh
    • Organizer
      The workshop "Development and Adult Neurogenesis in the Central Nervous System".
    • Place of Presentation
      Baeza, Spain
    • Year and Date
      2015-10-05 – 2015-10-07
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Modeling the regionality of embryonic neural development in epiblast stem cells.2015

    • Author(s)
      Kondoh H
    • Organizer
      Current trends in biomedicine workshop: Development and adult neurogenesis in the central nervous system.
    • Place of Presentation
      Universidad International de Andalucia, Baeza, Spain
    • Year and Date
      2015-10-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sox2-Pax6 interaction leading to lens development even via non-canonical pathway2015

    • Author(s)
      Kondoh H
    • Organizer
      Symposium: An eye on development
    • Place of Presentation
      University of Heidelberg, Heidelberg, Germany
    • Year and Date
      2015-06-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Two classes of Sox2-partner factor interaction involved in developmental gene regulation, as indicated by genome-wide ChIP-seq analaysis.2015

    • Author(s)
      Kondoh H, Matsuda, K, Mikami T, Andrabi M, Yamaguchi K, Shigenobu S.
    • Organizer
      48th Annual meetin of the Japanese Society of Developmental Biologists.
    • Place of Presentation
      Tsukuba Convention Center, Tsukuba, Ibaragi, Japan
    • Year and Date
      2015-06-02
    • Related Report
      2015 Annual Research Report
  • [Presentation] Regionally specific mechanisms of neural plate development.2014

    • Author(s)
      H. Kondoh
    • Organizer
      18th International Conference of the International Society of Differentiation.
    • Place of Presentation
      London, UK
    • Year and Date
      2014-11-02 – 2014-11-05
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] An ensemble of Sox-partner interactions determines genomic targets and specify cell states.2014

    • Author(s)
      H. Kondoh
    • Organizer
      4th International SOX Research Conference
    • Place of Presentation
      Cleveland, Ohio, USA
    • Year and Date
      2014-09-08 – 2014-09-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] Dynamics of gene regulatory network centered by Sox2 during embryogenesis.2014

    • Author(s)
      H. Kondoh
    • Organizer
      KSU International Symposium: Cutting-edge of Life Sciences
    • Place of Presentation
      京都産業大学、京都市
    • Year and Date
      2014-05-30 – 2014-05-31
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] The role of Tbx6 in the derivation of mesodermal tissue from the axial stem cells.2014

    • Author(s)
      Takemoto, H. Kondoh
    • Organizer
      47th Annual Meeting of Japanese Society of Developmental Biologists
    • Place of Presentation
      ウィンク愛知、名古屋市
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] Multiple enhancers of the Sox2 locus distributed within a 200 kb region in the chicken genome.2014

    • Author(s)
      M. Uchikawa, R. Okamoto, H. Kondoh
    • Organizer
      47th Annual Meeting of Japanese Society of Developmental Biologists
    • Place of Presentation
      ウィンク愛知、名古屋市
    • Year and Date
      2014-05-27 – 2014-05-30
    • Related Report
      2014 Annual Research Report
  • [Book] Sox2: Biology and Role in Development and Disease2015

    • Author(s)
      Kondoh H and Lovell-Badge R (Eds)
    • Total Pages
      327
    • Publisher
      Academic Press/Elsevier
    • Related Report
      2015 Annual Research Report
  • [Remarks] Laboratory of Developmental Biology 発生生物学研究室

    • URL

      https://www.cc.kyoto-su.ac.jp/~i1659235/HKlab/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 再生医療の地図を描くエピブラスト幹細胞

    • URL

      https://www.kyoto-su.ac.jp/faculty/professors/nls/ahcetq00000023w2-att/st_kondo.pdf

    • Related Report
      2016 Annual Research Report
  • [Remarks] SOX2に導かれた、発生の遺伝子制御の道

    • URL

      https://www.kyoto-su.ac.jp/graduate/g_ls/kenkyu/kondo.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 教員紹介 近藤寿人

    • URL

      https://www.kyoto-su.ac.jp/graduate/g_ls/kyoin/kondo.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] SOX2に導かれた発生の遺伝子制御への道

    • URL

      https://www.kyoto-su.ac.jp/graduate/g_ls/kenkyu/kondo.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 胚発生を制御する細胞間シグナルと遺伝子調節

    • URL

      http://www.kyoto-su.ac.jp/graduate/g_ls/kyoin/kondo.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] Laboratory of Developmental Biology

    • URL

      http://www.cc.kyoto-su.ac.jp/~yishii/devbiol/index.html

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2022-02-22  

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