| Budget Amount *help |
¥40,430,000 (Direct Cost: ¥31,100,000、Indirect Cost: ¥9,330,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2016: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2015: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2014: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
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| Outline of Final Research Achievements |
Light is essential for photosynthesis, but the amounts of light that exceed an organism's assimilation capacity cause photooxidative stress. Photosynthetic organisms minimize such potential harm through protection mechanisms referred to as non-photochemical quenching. One such mechanism called qE quenching is readily activated under high-light conditions. In this study, we investigated the details of the induction mechanism of qE quenching in a green alga Chlamydomonas reinhardtii. LHCSRs have been proposed to mediate qE quenching in C. reinhardtii when grown under high-light conditions. We showed that qE requires blue-light perception by phototropin, LHCSRs gene expression, and light dissipation by LHCSRs transported in the chloroplast. We further showed that the phot mutation can be suppressed by mutations in an E3 ubiquitin ligase CUL4-DDB1(DET1), suggesting this DET1-dependent E3 ubiquitin ligase plays a vital role in the blue light signal transduction in green algae.
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