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Regulation of photosynthesis under variable light

Research Project

Project/Area Number 26251033
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Minagawa Jun  基礎生物学研究所, 環境光生物学研究部門, 教授 (80280725)

Co-Investigator(Kenkyū-buntansha) 得津 隆太郎  基礎生物学研究所, 環境光生物学研究部門, 助教 (60613940)
Research Collaborator Kamada Konomi  
Yonezawa Harumi  
Kojima Youko  
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥40,430,000 (Direct Cost: ¥31,100,000、Indirect Cost: ¥9,330,000)
Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2016: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2015: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2014: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Keywords光合成 / 植物 / 藻類 / 環境適応
Outline of Final Research Achievements

Light is essential for photosynthesis, but the amounts of light that exceed an organism's assimilation capacity cause photooxidative stress. Photosynthetic organisms minimize such potential harm through protection mechanisms referred to as non-photochemical quenching. One such mechanism called qE quenching is readily activated under high-light conditions. In this study, we investigated the details of the induction mechanism of qE quenching in a green alga Chlamydomonas reinhardtii. LHCSRs have been proposed to mediate qE quenching in C. reinhardtii when grown under high-light conditions. We showed that qE requires blue-light perception by phototropin, LHCSRs gene expression, and light dissipation by LHCSRs transported in the chloroplast. We further showed that the phot mutation can be suppressed by mutations in an E3 ubiquitin ligase CUL4-DDB1(DET1), suggesting this DET1-dependent E3 ubiquitin ligase plays a vital role in the blue light signal transduction in green algae.

Academic Significance and Societal Importance of the Research Achievements

ほぼすべての光合成生物は直射日光を苦手としていることが明らかになり、光合成生物が過剰光をいかに凌いで光合成を行うかの重要性の認識が高まっている。地球上の二酸化炭素固定量の約半分は水中の光合成生物(藻類)が担うが、多くの藻類では過剰光をしのぐ仕組み(qEクエンチング)を、必要に応じて「誘導」していることが知られている。本研究はこの誘導の根幹を分子レベルで初めて明らかにした。フォトトロピンという陸上植物では光屈性等で知られる青色光受容体が藻類では光防御の起点となることがわかった。さらにその下流のシグナル伝達因子も明らかになり、本研究により光合成の環境適応研究は細胞生物学の領域に入った。

Report

(5 results)
  • 2018 Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (44 results)

All 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (4 results) Journal Article (12 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 12 results,  Open Access: 6 results,  Acknowledgement Compliant: 5 results) Presentation (25 results) (of which Int'l Joint Research: 15 results,  Invited: 19 results) Book (2 results) Remarks (1 results)

  • [Int'l Joint Research] フリードリヒ・シラー大学イェーナ(ドイツ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] ミュンスター大学(ドイツ)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] 原子力・代替エネルギー庁(フランス)

    • Related Report
      2014 Annual Research Report
  • [Int'l Joint Research] ハンガリー科学アカデミー(ハンガリー)

    • Related Report
      2014 Annual Research Report
  • [Journal Article] LHCSR1-dependent fluorescence quenching is mediated by excitation energy transfer from LHCII to photosystem I inChlamydomonas reinhardtii2018

    • Author(s)
      Kosuge Kotaro、Tokutsu Ryutaro、Kim Eunchul、Akimoto Seiji、Yokono Makio、Ueno Yoshifumi、Minagawa Jun
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: in press Issue: 14 Pages: 3722-3727

    • DOI

      10.1073/pnas.1720574115

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Investigation on the Thermodynamic Dissociation Kinetics of Photosystem II Supercomplexes To Determine the Binding Strengths of Light-Harvesting Complexes2018

    • Author(s)
      Kim Eunchul、Tokutsu Ryutaro、Minagawa Jun
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 122 Issue: 5 Pages: 1627-1630

    • DOI

      10.1021/acs.jpcb.7b12417

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Algal photoprotection is regulated by the E3 ligase CUL4?DDB1DET12018

    • Author(s)
      Aihara Yusuke、Fujimura-Kamada Konomi、Yamasaki Tomohito、Minagawa Jun
    • Journal Title

      Nature Plants

      Volume: 5 Issue: 1 Pages: 34-40

    • DOI

      10.1038/s41477-018-0332-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A blue light photoreceptor mediates the feedback regulation of photosynthesis.2016

    • Author(s)
      Petroutsos D, Tokutsu R, Maruyama S, Flori S, Greiner A, Magneschi L, Cusant L, Kottke T, Mittag M, Hegemann P, Finazzi G and Minagawa J.
    • Journal Title

      Nature

      Volume: 537 Issue: 7621 Pages: 563-566

    • DOI

      10.1038/nature19358

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Eyespot-dependent determination of the phototactic sign in Chlamydomonas reinhardtii2016

    • Author(s)
      Ueki, N., Ide, T., Mochiji, S., Kobayashi, Y., Tokutsu, R., Ohnishi, N., Yamaguchi, K., Shigenobu, S., Tanaka, K., Minagawa, J., Hisabori, T., Hirono, M., Wakabayashi, K.
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 印刷中 Issue: 19 Pages: 5266-5304

    • DOI

      10.1073/pnas.1525538113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Heat induction of cyclic electron flow around photosystem I in the symbiotic dinoflagellate Symbiodinium.2016

    • Author(s)
      Aihara, Y., Takahashi, S., Minagawa, J.
    • Journal Title

      Plant Physiol.

      Volume: 171 Issue: 1 Pages: 522-529

    • DOI

      10.1104/pp.15.01886

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PSBR is required for efficient binding of LHCSR3 to photosystem II - light-harvesting supercomplexes in Chlamydomonas reinhardtii.2015

    • Author(s)
      9.Xue, H., Tokutsu, R., Bergner, S., Scholz, M., Minagawa, J., Hippler, M.
    • Journal Title

      Plant Physiol

      Volume: 167 Issue: 4 Pages: 1566-1578

    • DOI

      10.1104/pp.15.00094

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Dynamic regulation of photosynthesis in Chlamydomonas reinhardtii.2015

    • Author(s)
      Minagawa, J., Tokutsu, R.
    • Journal Title

      Plant J.

      Volume: 82 Issue: 3 Pages: 413-428

    • DOI

      10.1111/tpj.12805

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Diversification of light harvesting complex gene family via intra- and intergenic duplications in the coral symbiotic alga Symbiodinium.2015

    • Author(s)
      Maruyama, S., Shoguchi, E., Satoh, N., Minagawa, J.
    • Journal Title

      PLoS One

      Volume: 10 Issue: 3 Pages: 1-15

    • DOI

      10.1371/journal.pone.0119406

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Chloroplast remodeling during state transitions in Chlamydomonas reinhardtii as revealed by non-invasive techniques in vivo.2014

    • Author(s)
      Nagy, G., Unnep, R., Zsiros, O., Tokutsu, R., Takizawa, K., Porcar, L., Moyet, L., Petroutsos, D., Garab, G., Finazzi, G., Minagawa, J.
    • Journal Title

      Proc. Natl. Acad. Sci. U. S. A.

      Volume: 111 Issue: 13 Pages: 5042-5047

    • DOI

      10.1073/pnas.1322494111

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Transcriptional regulation of the stress-responsive light harvesting complex genes in Chlamydomonas reinhardtii2014

    • Author(s)
      Maruyama S, Tokutsu R, Minagawa J
    • Journal Title

      Plant and Cell Physiology

      Volume: 55 Issue: 7 Pages: 1304-1310

    • DOI

      10.1093/pcp/pcu068

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Biochemical Characterization of Photosystem I-Associated Light-Harvesting Complexes I and II Isolated from State 2 Cells of Chlamydomonas reinhardtii2014

    • Author(s)
      H. Takahashi, A. Okamuro, J. Minagawa and Y. Takahashi
    • Journal Title

      Plant Cell Physiol

      Volume: 55 Issue: 8 Pages: 1437-1449

    • DOI

      10.1093/pcp/pcu071

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Algal photoprotection is mediated by phototropin and CUL4-DDB1DET12018

    • Author(s)
      Jun Minagawa
    • Organizer
      北京大学セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Algal photoprotection Mechanism and Signal Transduction2018

    • Author(s)
      Jun Minagawa
    • Organizer
      " International Symposium on photosynthesis and chloroplast biogenesis "
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ROC75 is an Attenuator for the Circadian Clock that Controls LHCSR3 Expression2017

    • Author(s)
      Jun Minagawa,Yousef Yari Kamrani,Takuya Matsuo,Maria Mittag
    • Organizer
      The 18th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Photoprotection in Chlamydomonas : Mechanism and signal transduction2017

    • Author(s)
      Jun Minagawa
    • Organizer
      1st Asia-Oceania International Congress on Photosynthesis (AOICP2018)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Investigation on Spilover upon Open/Closed PSll States Using PAM-Spectrometer2017

    • Author(s)
      Eunchul Kim,Jun Minagawa
    • Organizer
      第9回日本光合成学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Regulation of photosynthesisby the power of proton2017

    • Author(s)
      皆川純
    • Organizer
      Japanese society of Plant Physiology
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The molecular functions of LHCII phosphorylation during state transitions2017

    • Author(s)
      得津隆太郎、Kim Eunchul、秋本誠志、横野牧生、鎌田このみ、大西紀和、皆川純
    • Organizer
      日本植物生理学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] サンゴ共生褐虫藻におけるPSI-LHCI超複合体の機能構造解析2017

    • Author(s)
      加藤弘樹、得津隆太郎、河合(久保田)寿子、Ray Burton-Smith、皆川純
    • Organizer
      日本植物生理学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] An Innovative Way to Isolate Stable PSII Supercomplexes2017

    • Author(s)
      Akimasa Watanabe, Ryutaro Tokutsu, Eunchul Kim, Burton-Smith Ray, Jun Minagawa
    • Organizer
      日本植物生理学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 褐虫藻Symbiodinium minutumにおける光化学系I複合体の機能構造解析2017

    • Author(s)
      加藤弘樹、得津隆太郎、河合(久保田)寿子、Ray Burton-Smith、皆川純
    • Organizer
      日本光合成学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Amphipolを用いたPSII-LHCII Supercomplexの精製2017

    • Author(s)
      渡邉 顕正, 得津 隆太郎, Burton-Smith Ray, Eunchul Kim, 皆川 純
    • Organizer
      日本光合成学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 光合成の再最適化を目指す2016

    • Author(s)
      皆川純
    • Organizer
      植物科学シンポジウム
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Dissipation of excess light energy in Chlamydomonas reinhardtii2015

    • Author(s)
      Minagawa, J.
    • Organizer
      Japan-Germany Binational Seminar
    • Place of Presentation
      かんぽの宿熱海本館(神奈川県熱海市)
    • Year and Date
      2015-03-22
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dissipation of excess light energy in Chlamydomonas reinhardtii2015

    • Author(s)
      Minagawa, J.
    • Organizer
      CRL International forum : Tokyo Tech-HHU Dusseldorf Joint Symposium on Photosynthesis as a New Chemical Resource
    • Place of Presentation
      東京工業大学田町キャンパス(東京都港区)
    • Year and Date
      2015-03-04
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dissipation of Excess Light Energy in Chlamydomonas reinhardtii2015

    • Author(s)
      皆川純
    • Organizer
      山田コンファレンス
    • Place of Presentation
      奈良県、奈良市、奈良春日野国際フォーラム甍
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dissipation of Excess Light Energy in Chlamydomonas reinhardtii2015

    • Author(s)
      皆川純
    • Organizer
      International Conference on Microalgae and Biofuel
    • Place of Presentation
      トルコ、イスタンブール、コチ大学
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dissipation of Excess Light Energy in Chlamydomonas reinhardtii2015

    • Author(s)
      皆川純
    • Organizer
      Asia and Oceania Conference on Photobiology
    • Place of Presentation
      台湾、台北、アカデミア・シニカ
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 光化学系超複合体のリモデリングによる環境適応2014

    • Author(s)
      皆川純
    • Organizer
      IGERセミナー
    • Place of Presentation
      名古屋大学(愛知県名古屋市)
    • Year and Date
      2014-12-11
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Dynamic remodeling of photosynthetic supercomplexes during environmental acclimation2014

    • Author(s)
      Minagawa, J.
    • Organizer
      Japan-France Binational Symposium
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2014-10-27
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Non-photochemical quenching in Chlamydomonas2014

    • Author(s)
      Minagawa, J.
    • Organizer
      IPR seminar
    • Place of Presentation
      大阪大学(大阪府吹田市)
    • Year and Date
      2014-10-24
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A new model for state transitions in green algae as revealed by a study on the chloroplast remodeling in vivo2014

    • Author(s)
      Minagawa, J.
    • Organizer
      Japanese-Finnish Bionational Seminar
    • Place of Presentation
      定山渓万世閣ホテルミリオーネ(北海道札幌市)
    • Year and Date
      2014-10-10
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Regulation of NPQ induction in Chlamydomonas reinhardtii2014

    • Author(s)
      Minagawa, J
    • Organizer
      Gordon Research Conference on Photosynthesis
    • Place of Presentation
      West Dover, VT, USA
    • Year and Date
      2014-08-11
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 藻類の光合成効率はいかに達成されているのか2014

    • Author(s)
      皆川純
    • Organizer
      産学連携コンソーシアム
    • Place of Presentation
      東京大学(東京都文京区)
    • Year and Date
      2014-07-25
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Chloroplast remodeling during state transitions in a green alga Chlamydomonas reinhardtii2014

    • Author(s)
      Minagawa, J.
    • Organizer
      The 4th International Conference on Algal Biomass, Biofuels and Bioproducts
    • Place of Presentation
      Santa Fe, NM, USA
    • Year and Date
      2014-06-16
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dynamic aspects of photosynthesis2014

    • Author(s)
      Minagawa, J.
    • Organizer
      The 16th International conference on the cell and molecular biology of Chlamydomonas
    • Place of Presentation
      Asilomar, CA, USA
    • Year and Date
      2014-06-10
    • Related Report
      2014 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] Advances in Photosynthesis and Respiration Vol.40 : Non-Photochemical Quenching and Energy Dissipation in Plants, Algae, and Cyanobacteria2014

    • Author(s)
      Finazzi, G., Minagawa, J.
    • Total Pages
      649
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] Advances in Plant Biology Vol. 5 : Plastid Biology2014

    • Author(s)
      Johnson, G. N., Cardol, P., Minagawa, J., Finazzi, G.
    • Total Pages
      589
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Remarks] 基礎生物学研究所環境光生物学研究部門

    • URL

      http://www.nibb.ac.jp/photo/index.html

    • Related Report
      2017 Annual Research Report

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Published: 2014-04-04   Modified: 2020-03-30  

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