Molecular basis for meiotic recombination of homologous chromosomes

• Project/Area Number: 26251037
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Chromosome dynamics
• Research Institution: Osaka University
• Principal Investigator: HIRAOKA Yasushi 大阪大学, 生命機能研究科, 教授 (10359078)
• Co-Investigator(Renkei-kenkyūsha): ASAKAWA Haruhiko 大阪大学, 生命機能研究科, 准教授 (70399533)
• Research Collaborator: CHIKASHIGE Yuji 国立研究開発法人情報通信研究機構, 未来ICT研究所, 主任研究員 (60359081) ; OBUSE Chikashi 北海道大学, 先端生命科学研究院, 教授 (00273855)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥40,560,000 (Direct Cost: ¥31,200,000、Indirect Cost: ¥9,360,000); Fiscal Year 2016: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000); Fiscal Year 2015: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000); Fiscal Year 2014: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
• Keywords: 細胞 / 染色体

Outline of Final Research Achievements

Imaging of meiotic chromatin: Using super-resolution microscopy, we found that meiotic cohesion-based chromatin structures are necessary for homologous chromosome pairing. We also found that chromatin decompaction occurs during meiotic DNA replication and that the decompaction requires acetylation of histone H4. In addition, we developed a new method to visualize histone modification in living cells.
Regulatory mechanisms of meiosis: We found that a component of the nuclear pore complex, Nup132, plays an important role in progression of meiosis, and that two APC/C activators regulate progression of meiosis. We also revealed that a nuclear membrane protein Lem2 augments centromeric heterochromatin in a nutrition-dependent manner.

Research Products

• [Journal Article] Spatial organization of the Schizosaccharomyces pombe genome within the nucleus. (2017)
  • Author(s): Matsuda A, Asakawa H, Haraguchi T, Hiraoka Y.
  • Journal Title: Yeast. Volume: 34 Issue: 2 Pages: 55-66
  • DOI: 10.1002/yea.3217
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] In vitro reconstitution and biochemical analyses of theSchizosaccharomyces pombe nucleosome. (2017)
  • Author(s): Masako Koyama, Wataru Nagakura, Hiroki Tanaka, Tomoya Kujirai, Yuji Chikashige, Tokuko Haraguchi, Yasushi Hiraoka, Hitoshi Kurumizaka.
  • Journal Title: Biochemical and biophysical research communications. Volume: 482 Issue: 4 Pages: 896-901
  • DOI: 10.1016/j.bbrc.2016.11.130
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Lipid droplet dynamics during Schizosaccharomyces pombe sporulation and their role in spore survival. (2017)
  • Author(s): Yang HJ, Osakada H, Kojidani T, Haraguchi T, Hiraoka Y.
  • Journal Title: Biology open Volume: 6 Pages: 217-222
  • DOI: 10.1242/bio.022384
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fission yeast APC/C activators Slp1 and Fzr1 sequentially trigger two consecutive nuclear divisions during meiosis. (2017)
  • Author(s): Chikashige Y, Yamane M, Okamasa K, Osakada H, Tsutsumi C, Nagahama Y, Fukuta N, Haraguchi T, Hiraoka Y.
  • Journal Title: FEBS letters. Volume: 591 Issue: 7 Pages: 1029-1040
  • DOI: 10.1002/1873-3468.12612
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A cohesion-based platform for homologous chromosome pairing in meiosis (2016)
  • Author(s): Da-Qiao Ding, Tokuko Haraguchi, Yasushi Hiraoka
  • Journal Title: Current Genetics Volume: 印刷中 Issue: 3 Pages: 499-502
  • DOI: 10.1007/s00294-016-0570-x
  • Peer Reviewed
• [Journal Article] Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions. (2016)
  • Author(s): Tange Y, Chikashige Y, Takahata S, Kawakami K, Higashi M, Mori C, Kojidani T, Hirano Y, Asakawa H, Murakami Y, Haraguchi T, Hiraoka Y.
  • Journal Title: Genes to Cells Volume: 21 Issue: 8 Pages: 812-832
  • DOI: 10.1111/gtc.12385
  • NAID: 120005906898
  • Peer Reviewed / Open Access
• [Journal Article] Identification of Conserved MEL-28/ELYS Domains with Essential Roles in Nuclear Assembly and Chromosome Segregation. (2016)
  • Author(s): Gomez-Saldivar G, Fernandez A, Hirano Y, Mauro M, Lai A, Ayuso C, Haraguchi T, Hiraoka Y, Piano F, Askjaer P.
  • Journal Title: PLoS genetics. Volume: 12 Issue: 6 Pages: e1006131-e1006131
  • DOI: 10.1371/journal.pgen.1006131
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Depletion of autophagy receptor p62/SQSTM1 enhances the efficiency of gene delivery in mammalian cells. (2016)
  • Author(s): Tsuchiya M, Ogawa H, Koujin T, Kobayashi S, Mori C, Hiraoka Y, Haraguchi T.
  • Journal Title: FEBS letters. Volume: 590 Issue: 16 Pages: 2671-2680
  • DOI: 10.1002/1873-3468.12262
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A genetically encoded probe for live-cell imaging of H4K20 monomethylation (2016)
  • Author(s): Sato Y., Kujirai T., Arai R., Asakawa H., Ohtsuki C., Horikoshi N., Yamagata K., Ueda J., Nagase T., Haraguchi T., Hiraoka Y., Kimura A., Kurumizaka H., Kimura, H.
  • Journal Title: J. Mol. Biol. Volume: 428 Issue: 20 Pages: 3885-3902
  • DOI: 10.1016/j.jmb.2016.08.010
  • Peer Reviewed / Open Access
• [Journal Article] Borna Disease Virus Assembles Porous Cage-like Viral Factories in the Nucleus. (2016)
  • Author(s): Hirai Y, Hirano Y, Matsuda A, Hiraoka Y, Honda T, Tomonaga K.
  • Journal Title: The Journal of biological chemistry. Volume: 291 Issue: 50 Pages: 25789-25798
  • DOI: 10.1074/jbc.m116.746396
  • Peer Reviewed / Open Access
• [Journal Article] Uniquely designed nuclear structures of lower eukaryotes (2016)
  • Author(s): Masaaki Iwamoto, Yasushi Hiraoka, Tokuko Haraguchi
  • Journal Title: Current Opinion in Cell Biology Volume: 40 Pages: 66-73
  • DOI: 10.1016/j.ceb.2016.02.019
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Shugoshin forms a specialized chromatin domain at subtelomeres that regulates transcription and replication timing. (2016)
  • Author(s): Tashiro S, Handa T, Matsuda A, Ban T, Takigawa T, Miyasato K, Ishii K, Kugou K, Ohta K, Hiraoka Y, Masukata H, Kanoh J.
  • Journal Title: Nature Commun. Volume: 7 Issue: 1 Pages: 10393-10393
  • DOI: 10.1038/ncomms10393
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Histone H3K36 trimethylation is essential for multiple silencing mechanism in fission yeast. (2016)
  • Author(s): Shota Suzuki, Hiroaki Kato, Yutaka Suzuki, Yuji Chikashige, Yasushi Hiraoka, Hiroshi Kimura, Koji Nagao, Chikashi Obuse, Shinya Takahata and Yota Murakami
  • Journal Title: Nucleic Acids Research Volume: 44 (9) Issue: 9 Pages: 4147-4162
  • DOI: 10.1093/nar/gkw008
  • NAID: 120005819302
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Virtual nuclear envelope breakdown and its regulators in fission yeast meiosis (2016)
  • Author(s): Asakawa H, Yang H, Hiraoka Y, Haraguchi T.
  • Journal Title: Front. Cell Dev. Volume: 4 Pages: 1-8
  • DOI: 10.3389/fcell.2016.00005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A nucleoporin that facilitates meiotic kinetochore reorganization (2016)
  • Author(s): Yang H-J, Haraguchi T, Hiraoka Y
  • Journal Title: Cell Cycle Volume: 15 Issue: 3 Pages: 307-308
  • DOI: 10.1080/15384101.2015.1125237
  • Peer Reviewed / Open Access
• [Journal Article] Meiotic cohesion-based chromosome structure is essential for homologous chromosome pairing in Schizosaccharomyces pombe (2015)
  • Author(s): Ding DQ, Matsuda A, Okamasa K, Nagahama Y, Haraguchi T, Hiraoka Y
  • Journal Title: Chromosoma Volume: special issue Issue: 2 Pages: 1-10
  • DOI: 10.1007/s00412-015-0551-8
  • Peer Reviewed / Open Access
• [Journal Article] Cellular economy in fission yeast cells continuously cultured with limited nitrogen resources (2015)
  • Author(s): Yuji Chikashige, Shinichi Arakawa, Kenji Leibnitz, Chihiro Tsutsumi, Chie Mori, Hiroko Osakada, Masayuki Murata, Tokuko Haraguchi, Yasushi Hiraoka
  • Journal Title: Scientific Reports Volume: 5 Issue: 1
  • DOI: 10.1038/srep15617
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly. (2015)
  • Author(s): Yang H-J, Asakawa H, Haraguchi T, Hiraoka Y.
  • Journal Title: J. Cell Biol. Volume: 211 Issue: 2 Pages: 295-308
  • DOI: 10.1083/jcb.201501035
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fission yeast meets a legend in Kobe: report of the Eighth International Fission Yeast Meeting. (2015)
  • Author(s): Asakawa H, Yamamoto TG, Hiraoka Y.
  • Journal Title: Genes Cells Volume: 20 Issue: 12 Pages: 967-971
  • DOI: 10.1111/gtc.12307
  • Peer Reviewed / Open Access
• [Journal Article] Selective autophagic receptor p62 regulates the abundance of transcriptional coregulator ARIP4 during nutrient starvation (2015)
  • Author(s): Megumi Tsuchiya, Shin Isogai, Hiroaki Taniguchi, Hidehito Tochio, Masahiro Shirakawa, Ken-ichirou Morohashi, Yasushi Hiraoka, Tokuko Haraguchi & Hidesato Ogawa
  • Journal Title: Scientific Report Volume: 5 Issue: 1 Pages: 14498-14498
  • DOI: 10.1038/srep14498
  • NAID: 120005820894
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The nuclear pore complex acts as a master switch for nuclear and cell differentiation (2015)
  • Author(s): Masaaki Iwamoto, Yasushi Hiraoka, Tokuko Haraguchi
  • Journal Title: Communicative and Integrative Biology Volume: 8 Issue: 4 Pages: e1056950-e1056950
  • DOI: 10.1080/19420889.2015.1056950
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Histone H4 acetylation required for chromatin decompaction during DNA replication (2015)
  • Author(s): Ruan K, Yamamoto TG, Asakawa H, Chikashige Y, Kimura H, Masukata H, Haraguchi T, Hiraoka Y.
  • Journal Title: Sci Rep Volume: 5 Issue: 1 Pages: 1-10
  • DOI: 10.1038/srep12720
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Highly condensed chromatins are formed adjacent to subtelomeric and decondensed silent chromatin in fission yeast. (2015)
  • Author(s): Matsuda A, Chikashige Y, Ding DQ, Ohtsuki C, Mori C, Asakawa H, Kimura H, Haraguchi T, Hiraoka Y.
  • Journal Title: Nature Communication Volume: 6 Issue: 1 Pages: 1-12
  • DOI: 10.1038/ncomms8753
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Chromosome Scaffold is a Double-Stranded Assembly of Scaffold Proteins. (2015)
  • Author(s): Poonperm R, Takata H, Hamano T, Matsuda A, Uchiyama S, Hiraoka Y, Fukui K
  • Journal Title: Sci. Rep. Volume: 5 Issue: 1 Pages: 11916-11916
  • DOI: 10.1038/srep11916
  • NAID: 120006995010
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Recent advancements in structured-illumination microscopy toward live-cell imaging. (2015)
  • Author(s): Hirano Y, Matsuda A, Hiraoka Y
  • Journal Title: Microscopy (Oxf) Volume: 64 Issue: 4 Pages: 237-249
  • DOI: 10.1093/jmicro/dfv034
  • Peer Reviewed / Open Access
• [Journal Article] Uncleavable Nup98-Nup96 is functional in the fission yeast Schizosaccharomyces pombe (2015)
  • Author(s): Haruhiko Asakawa, Chie Mori, Chizuru Ohtsuki, Masaaki Iwamoto, Yasushi Hiraoka, Tokuko Haraguchi
  • Journal Title: FEBS Open Bio Volume: 5 Issue: 1 Pages: 508-514
  • DOI: 10.1016/j.fob.2015.06.004
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy. (2015)
  • Author(s): Kobayashi,S., Koujin, T., Kojidani, T., Osakada, H., Mori, C., Hiraoka, Y., Haraguchi, T.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 印刷中 Issue: 22 Pages: 7027-7032
  • DOI: 10.1073/pnas.1501235112
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Biased assembly of the nuclear pore complex is required for somatic and germline nuclear differentiation in Tetrahymena (2015)
  • Author(s): Masaaki Iwamoto, Takako Koujin, Hiroko Osakada, Chie Mori, Tomoko Kojidani, Atsushi Matsuda, Haruhiko Asakawa, Yasushi Hiraoka, Tokuko Haraguchi
  • Journal Title: Journal of Cell Science Volume: 128 Pages: 1812-1823
  • DOI: 10.1242/jcs.167353
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fission Yeast Scp3 Potentially Maintains Microtubule Orientation through Bundling (2015)
  • Author(s): Ozaki K, Chikashige Y, Hiraoka Y, Matsumoto T.
  • Journal Title: PLoS One Volume: 10 Issue: 3 Pages: e0120109-e0120109
  • DOI: 10.1371/journal.pone.0120109
  • NAID: 120005745675
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Meiotic nuclear movements in fission yeast are regulated by the transcription factor Mei4 downstream of a Cds1-dependent replication checkpoint pathway. (2014)
  • Author(s): Ruan K, Yamamoto TG, Asakawa H, Chikashige Y, Masukata H, Haraguchi T, Hiraoka Y.
  • Journal Title: Genes Cells Volume: 20 Issue: 3 Pages: 160-172
  • DOI: 10.1111/gtc.12207
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Externally Controllable Molecular Communication (2014)
  • Author(s): Tadashi Nakano, Shouhei Kobayashi, Tatsuya Suda, Yutaka Okaie, Yasushi Hiraoka, Tokuko Haraguchi
  • Journal Title: IEEE Journal of Selected Areas in Communications Volume: 32 Issue: 12 Pages: 2417-2431
  • DOI: 10.1109/jsac.2014.2367667
  • Peer Reviewed / Open Access
• [Journal Article] Externally Controllable Molecular Communication Systems. (2014)
  • Author(s): Nakano, T., Kobayashi, S., Suda, T., Okaie, Y., Hiraoka, Y., Haraguchi, T.
  • Journal Title: IEEE Journal of Selected Areas in Communications Volume: 32 Pages: 2417-2431
  • DOI: 10.1145/2619955.2619971
  • Peer Reviewed / Open Access
• [Journal Article] Cooperative Target Tracking by a Mobile Bionanosensor Network (2014)
  • Author(s): Okaie Y, Nakano T, Hara T, Obuchi T, Hosoda K, Hiraoka Y, Nishio S.
  • Journal Title: IEEE Transactions on Nanobioscience Volume: 13 Issue: 3 Pages: 267-277
  • DOI: 10.1109/tnb.2014.2343237
  • Peer Reviewed
• [Journal Article] Chromosomes Rein Back the Spindle Pole Body during Horsetail Movement in Fission Yeast Meiosis (2014)
  • Author(s): Yuji Chikashige, Miho Yamane, Kasumi Okamasa, Chie Mori, Noriko Fukuta, Atsushi Matsuda, Tokuko Haraguchi, Yasushi Hiraoka
  • Journal Title: Cell Structure and Function Volume: 39 Issue: 2 Pages: 93-100
  • DOI: 10.1247/csf.14007
  • NAID: 130004053902
  • ISSN: 0386-7196, 1347-3700
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A method of correlative light and electron microscopy for yeast cells (2014)
  • Author(s): Asakawa H, Hiraoka Y, Haraguchi T
  • Journal Title: Micron Volume: 61 Pages: 53-61
  • DOI: 10.1016/j.micron.2014.02.007
  • Peer Reviewed / Open Access
• [Presentation] A framework of meiotic chromosomes for homologous pairing. (2017)
  • Author(s): 平岡泰
  • Organizer: 広島大学クロマチン動態数理研究拠点"5th International Symposium of the Mathematics on Chromatin Live Synamics"
  • Place of Presentation: 東広島芸術文化ホールくらら（広島県東広島市）
  • Year and Date: 2017-03-07
  • Int'l Joint Research / Invited
• [Presentation] ヒストン不足が引き起こす染色体の不分離 (2017)
  • Author(s): 平岡泰
  • Organizer: よこはまＮＭＲ研究会 第56回ワークショップ「ヌクレオームとビッグデータ」
  • Place of Presentation: 理化学研究所（神奈川県横浜市）
  • Year and Date: 2017-03-03
  • Invited
• [Presentation] Chromatin-anchoring nuclear membrane proteins in fission yeast (2017)
  • Author(s): Hiraoka Y
  • Organizer: FMIシンポジウム
  • Place of Presentation: Friedrich Miescher Institute for Biomedical Research（Basel, Switzerland）
  • Year and Date: 2017-01-20
  • Int'l Joint Research / Invited
• [Presentation] A framework of meiotic chromosomes for homologous pairing. (2016)
  • Author(s): Hiraoka Y
  • Organizer: 2016 Workshop on the Molecular and Physical Biology of Chromosomes
  • Place of Presentation: Woods Hole Marine Biological Laboratory（Massachusetts, USA）
  • Year and Date: 2016-09-11
  • Int'l Joint Research / Invited
• [Presentation] 遺伝子のすがたを生きたまま見る (2016)
  • Author(s): 平岡泰
  • Organizer: 一般公開シンポジウム「遺伝子のすがた -カラダの中でおこる不思議-」
  • Place of Presentation: 早稲田大学国際会議場井深大記念ホール（東京都新宿区）
  • Year and Date: 2016-08-21
  • Invited
• [Presentation] Roles for inner nuclear membrane proteins Lem2 and Bqt4 in chromosome organization (2015)
  • Author(s): Yasushi Hiraoka
  • Organizer: 4th International Symposium of the Mathematics on Chromatin Live Dynamics
  • Place of Presentation: 広島市国際青年会館（広島県・広島市）
  • Year and Date: 2015-12-07
  • Int'l Joint Research / Invited
• [Presentation] Chromosomes and nuclear structures in fission yeast (2015)
  • Author(s): Yasushi Hiraoka
  • Organizer: Cold Spring Harbor Laboratory Meeting “Cell Biology of Yeasts”
  • Place of Presentation: Cold Spring Harbor(USA)
  • Year and Date: 2015-11-03
  • Int'l Joint Research / Invited
• [Presentation] Role for the fission yeast Lem2 protein in chromosome segregation and genome stability (2015)
  • Author(s): Yasushi Hiraoka
  • Organizer: Nuclear Transport Meeting
  • Place of Presentation: Sant Feliu de Guixols(Spain)
  • Year and Date: 2015-09-20
  • Int'l Joint Research / Invited
• [Presentation] Histone H4 acetylation required for chromatin loosening during DNA replication (2015)
  • Author(s): 阮琨、山本孝治、淺川東彦、原口徳子、平岡 泰
  • Organizer: 酵母遺伝学フォーラム第48回研究報告会
  • Place of Presentation: 広島大学（広島県・東広島市）
  • Year and Date: 2015-09-01
• [Presentation] Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore assembly (2015)
  • Author(s): Yang HJ, Asakawa H, Haraguchi T, Hiraoka Y
  • Organizer: EMBO Conference on Meiosis
  • Place of Presentation: Oxford（UK）
  • Year and Date: 2015-08-31
  • Int'l Joint Research
• [Presentation] A Role of Chromatin-Nuclear Membrane Protein Interaction on the Nuclear Envelope Assembly (2015)
  • Author(s): Tokuko Haraguchi, Shouhei Kobayashi, Takako Koujin, Hiroko Osakada,Tomoko Kojidani, Chie Mori, Yasushi Hiraoka
  • Organizer: International Symposium on Chromatin Structure, Dynamics, and Function
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県・淡路市）
  • Year and Date: 2015-08-25
  • Int'l Joint Research
• [Presentation] Chromosome Segregation is Impaired by Loss of Lem2 (2015)
  • Author(s): Yoshie Tange, Yuji Chikashige, Yasuhiro Hirano, Tokuko Haraguchi, Yasushi Hiraoka
  • Organizer: International Symposium on Chromatin Structure, Dynamics, and Function
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県・淡路市）
  • Year and Date: 2015-08-25
  • Int'l Joint Research / Invited
• [Presentation] Highly condensed chromatins are formed adjacent to subtelomeric and decondensed silent chromatin in fission yeast (2015)
  • Author(s): Atsushi Matsuda, Tokuko Haraguchi, Yasushi Hiraoka
  • Organizer: International Symposium on Chromatin Structure, Dynamics, and Function
  • Place of Presentation: 淡路夢舞台国際会議場（兵庫県・淡路市）
  • Year and Date: 2015-08-24
  • Int'l Joint Research
• [Presentation] Nup132 modulates meiotic spindle attachment in fission yeast by regulating kinetochore reassembly (2015)
  • Author(s): Hui-Ju Yang, Haruhiko Asakawa, Tokuko Haraguchi, Yasushi Hiraoka
  • Organizer: 8th International Fission Yeast Meeting
  • Place of Presentation: 生田神社会館（兵庫県・神戸市）
  • Year and Date: 2015-06-25
  • Int'l Joint Research
• [Presentation] Shugoshin forms a specialized chromatin domain at subtelomeres, repressing transcription and replication (2015)
  • Author(s): Sanki Tashiro, Tetsuya Handa, Atsushi Matsuda, Takuto Ban, Toru Takigawa, Kazumi Miyasato, Kojiro Ishii, Kazuto Kugou, Kunihiro Ohta, Yasushi Hiraoka, Hisao Masukata, Junko Kanoh
  • Organizer: 8th International Fission Yeast Meeting
  • Place of Presentation: 生田神社会館（兵庫県・神戸市）
  • Year and Date: 2015-06-24
  • Int'l Joint Research
• [Presentation] Chromosome segregation is impaired by loss of LEM-domain nuclear membrane protein Lem2 (2015)
  • Author(s): Yasushi Hiraoka, Yoshie Tange, Yuji Chikashige, Tokuko Haraguchi
  • Organizer: 8th International Fission Yeast Meeting
  • Place of Presentation: 生田神社会館（兵庫県・神戸市）
  • Year and Date: 2015-06-24
  • Int'l Joint Research / Invited
• [Presentation] Super-resolution microscopy reveals highly condensed chromatin adjacent to subtelomeric, decondensed silent chromatin (2015)
  • Author(s): Atsushi Matsuda, Tokuko Haraguchi, Yasushi Hiraoka
  • Organizer: 8th International Fission Yeast Meeting
  • Place of Presentation: 生田神社会館（兵庫県・神戸市）
  • Year and Date: 2015-06-24
  • Int'l Joint Research
• [Presentation] Replication timing control by telomere-adjacent nuclear localization in fission yeast (2015)
  • Author(s): Hisao Masukata, Yuta Nakamura, Hidesato Ogawa, Haruhiko Asakawa, Tatsuro S Takahashi, Takuro Nakagawa, Yasushi Hiraoka, Shiho Ogawa
  • Organizer: 8th International Fission Yeast Meeting
  • Place of Presentation: 生田神社会館（兵庫県・神戸市）
  • Year and Date: 2015-06-22
  • Int'l Joint Research / Invited
• [Presentation] Actin-dependent redistribution of lipid droplets confers the stress resistance of a yeast ascospore (2015)
  • Author(s): Yang HJ, Kojidani T, Osakada H, Asakawa H, Haraguchi T, Hiraoka Y
  • Organizer: Cold Spring Harbor Asia Conferences “Lipid Metabolism and Human Metabolic Disorders”
  • Place of Presentation: Suzhou(China)
  • Year and Date: 2015-06-04
  • Int'l Joint Research
• [Presentation] Roles for the inner nuclear membrane in genome stability (2015)
  • Author(s): 平岡 泰
  • Organizer: 蛋白研セミナー “Nuclear organization and actin-dependent mechanisms in genome stability”
  • Place of Presentation: 大阪大学蛋白質研究所（大阪府・吹田市）
  • Year and Date: 2015-05-18
• [Presentation] Meiotic function of histone H2A in fission yeast (2014)
  • Author(s): Yamamoto TG, Haraguchi T, Hiraoka Y.
  • Organizer: The 4D Nucleome 2014
  • Place of Presentation: Aki grando hotel, Hiroshima
  • Year and Date: 2014-12-18
• [Presentation] 染色体末端付近において転写・複製に抑制的なクロマチン構造がShugoshin 2依存的に形成される (2014)
  • Author(s): 田代 三喜, 松田 厚志, 半田 哲也, 坂 琢人, 宮里 和実, 石井 浩二郎, 久郷 和人, 太田 邦史, 平岡 泰, 升方 久夫 , 加納 純子
  • Organizer: 第32回染色体ワークショップ・第13回核ダイナミクス研究会
  • Place of Presentation: 安芸グランドホテル、広島県
  • Year and Date: 2014-12-17
• [Presentation] 分裂酵母サブテロメアのクロマチン高次構造 (2014)
  • Author(s): 松田 厚志, 木村 宏, 原口 徳子, 平岡 泰
  • Organizer: 第32回染色体ワークショップ・第13回核ダイナミクス研究会
  • Place of Presentation: 安芸グランドホテル、広島県
  • Year and Date: 2014-12-17
• [Presentation] 分裂酵母核内膜タンパク質Lem2とBqt4のクロマチン形成における役割 (2014)
  • Author(s): 平岡 泰
  • Organizer: 第32回染色体ワークショップ・第13回核ダイナミクス研究会
  • Place of Presentation: 安芸グランドホテル、広島県
  • Year and Date: 2014-12-16
• [Presentation] 超解像顕微鏡法によるボルナウイルス特異的核内構造体の構造および機能の解明 (2014)
  • Author(s): 平井 悠哉, 平野 泰弘, 本田 知之, 平岡 泰
  • Organizer: 第32回染色体ワークショップ・第13回核ダイナミクス研究会
  • Place of Presentation: 安芸グランドホテル、広島県
  • Year and Date: 2014-12-16
• [Presentation] 分裂酵母の減数分裂におけるヒストンH2A の役割 (2014)
  • Author(s): 山本孝治、原口徳子、平岡泰
  • Organizer: 第32回染色体ワークショップ.第13回核ダイナミクス研究会
  • Place of Presentation: 安芸グランドホテル、広島県
  • Year and Date: 2014-12-16
• [Presentation] 分裂酵母サブテロメアのクロマチン高次構造：脱凝縮したサイレント領域と凝縮した隣接領域 (2014)
  • Author(s): 松田 厚志、木村 宏、原口 徳子、平岡 泰
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、神奈川県
  • Year and Date: 2014-11-27
• [Presentation] 分裂酵母ネオセントロメアの減数分裂における機能特性 (2014)
  • Author(s): 荻山 友貴、淺川 東彦、平岡 泰、石井 浩二郎
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、神奈川県
  • Year and Date: 2014-11-25
• [Presentation] S期後期複製開始点の時空間的制御機構 (2014)
  • Author(s): 小川(長田） 志帆、高橋 達郎、中川 拓郎、小川 英知、淺川 東彦、平岡 泰、升方 久夫
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜、神奈川県
  • Year and Date: 2014-11-25
• [Presentation] 核内膜タンパク質のヘテロクロマチン形成における役割 (2014)
  • Author(s): 丹下 喜恵, 原口 徳子,平岡 泰
  • Organizer: 第87回日本生化学会大会
  • Place of Presentation: 国立京都国際会館、京都府
  • Year and Date: 2014-10-18
• [Presentation] 細胞核ダイナミクスの生細胞蛍光イメージング (2014)
  • Author(s): 平岡 泰
  • Organizer: 分子科学研究所研究会「細胞核内反応の分子科学」
  • Place of Presentation: 岡崎コンファレンスセンター、愛知県
  • Year and Date: 2014-09-27
  • Invited
• [Presentation] 減数分裂期におけるヒストンH2Aの機能解析 (2014)
  • Author(s): 山本孝治、原口徳子、平岡泰
  • Organizer: 第6回光塾
  • Place of Presentation: 未来ICT研究所、兵庫県
  • Year and Date: 2014-09-06
• [Presentation] 核膜内膜タンパク質が転写を調節する様子をありのまま見たい！ (2014)
  • Author(s): 平野泰弘、平岡泰
  • Organizer: 第6回光塾
  • Place of Presentation: 未来ICT研究所、兵庫県
  • Year and Date: 2014-09-06
• [Presentation] Live-cell imaging of chromosome dynamics in fission yeast (2014)
  • Author(s): 平岡 泰
  • Organizer: クロマチン動態数理研究拠点 MINI SYMPOSIUM
  • Place of Presentation: 広島大学、広島県
  • Year and Date: 2014-09-05
  • Invited
• [Presentation] GFP融合抗体断片の細胞内発現によるタンパク質翻訳後修飾の生細胞観察 (2014)
  • Author(s): 淺川東彦、大槻千鶴、佐藤優子、木村 宏、平岡 泰
  • Organizer: 酵母遺伝学フォーラム第47回研究報告会
  • Place of Presentation: 東京都、東京大学弥生講堂
  • Year and Date: 2014-09-03
• [Presentation] 分裂酵母核内膜タンパク質の染色体分配とヘテロクロマチン形成における役割 (2014)
  • Author(s): 丹下喜恵、原口徳子、平岡 泰
  • Organizer: 酵母遺伝学フォーラム第47回研究報告会
  • Place of Presentation: 東京都、東京大学弥生講堂
  • Year and Date: 2014-09-02
• [Presentation] 分裂酵母における減数分裂期DNA複製の停止による染色体の構造変化 (2014)
  • Author(s): Kun Ruan、山本孝治、浅川東彦、近重裕次、升方久夫、原口徳子、平岡 泰
  • Organizer: 酵母遺伝学フォーラム第47回研究報告会
  • Place of Presentation: 東京都、東京大学弥生講堂
  • Year and Date: 2014-09-02
• [Presentation] Inner nuclear membrane proteins affecting chromosome segregation in fission yeast (2014)
  • Author(s): Hiraoka Y
  • Organizer: FASEB Science Research Conference“Yeast Chromosome Structure, Replication and Segregation”
  • Place of Presentation: Steamboat Springs, USA
  • Year and Date: 2014-07-16
  • Invited
• [Presentation] 分裂酵母核内膜タンパク質Lem 2 の解析 (2014)
  • Author(s): 丹下喜恵、原口徳子、平岡泰
  • Organizer: 第2 回若手の会クロマチン動構造若手交流ワークショップ
  • Place of Presentation: 十勝サホロリゾート、北海道
  • Year and Date: 2014-07-02
• [Presentation] Nuclear Membranes and Heterochromatin in S. pombe (2014)
  • Author(s): Hiraoka Y
  • Organizer: Gordon Conference “Chromatin Structure & Function”
  • Place of Presentation: Bentley University, USA
  • Year and Date: 2014-06-11
  • Invited
• [Presentation] 分裂酵母のmRNA分布に占めるリボソームタンパク質遺伝子の特異なふるまい (2014)
  • Author(s): 近重裕次, 荒川伸一, Kenji Leibnitz, 森知栄, 堤千尋, 小坂田裕子, 福田紀子, 村田正幸, 原口徳子, 平岡泰
  • Organizer: 第66回日本細胞生物学会大会
  • Place of Presentation: 奈良県新公会堂、奈良県
  • Year and Date: 2014-06-11
• [Book] Fission Yeast: A Laboratory Manual (2016)
  • Author(s): Ding DQ, Hiraoka Y
  • Total Pages: 487
  • Publisher: Cold Spring Harbor Laboratory Press
• [Book] Fission Yeast: A Laboratory Manual (2016)
  • Author(s): Asakawa H, Ding DQ, Haraguchi T, Hiraoka Y
  • Total Pages: 487
  • Publisher: Cold Spring Harbor Laboratory Press
• [Book] 新・生細胞蛍光イメージング (2015)
  • Author(s): 原口徳子、木村宏、平岡泰（編著）
  • Total Pages: 352
  • Publisher: 共立出版
• [Remarks] 細胞核ダイナミクス研究室
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/general/lab/07/
• [Remarks] 細胞核ダイナミクス研究室 研究成果
  • URL: http://www.fbs.osaka-u.ac.jp/jpn/general/lab/07/result/
• [Remarks] Hiraoka Laboratory
  • URL: http://www.fbs.osaka-u.ac.jp/labs/hiraoka/index.html
• [Patent(Industrial Property Rights)] 核酸導入促進剤 (2016)
  • Inventor(s): 小川英知、原口徳子、土屋惠、平岡泰
  • Industrial Property Rights Holder: 大阪大学、情報通信研究機構
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-006937
  • Filing Date: 2016-01-18