Census of Deep Subseafloor Life: Biogeographical distribution of microbial life in Earth's interior

• Project/Area Number: 26251041
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: INAGAKI Fumio 国立研究開発法人海洋研究開発機構, 海洋掘削科学研究開発センター, 研究開発センター長代理 (50360748)
• Co-Investigator(Kenkyū-buntansha): 星野 辰彦 国立研究開発法人海洋研究開発機構, 高知コア研究所, 主任研究員 (30386619)
• Project Period (FY): 2014-06-27 – 2017-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥41,860,000 (Direct Cost: ¥32,200,000、Indirect Cost: ¥9,660,000); Fiscal Year 2016: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000); Fiscal Year 2014: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
• Keywords: 海底下生命圏 / アーキア / 微生物群集構造 / デジタルPCR / 海洋掘削科学 / IODP / 微生物群集構造解析 / 次世代シーケンサー / 遺伝子定量 / 16S rRNA遺伝子 / 海底堆積物

Outline of Final Research Achievements

To elucidate biogeographical distribution of deep microbial life in the global subseafloor sedimentary environment which accounts for ~70% of the Earth's surface, we extracted environmental genomic DNA from frozen core samples obtained by multiple scientific ocean drilling expeditions in the past decade and performed the comprehensive quantification and sequence analysis of 16S rRNA genes. Consequently, the proportion of Archaea in the global subseafloor sedimentary biosphere was estimated at ~37%, which is nearly consistent with archaeal abundance in the overlying marine environment. In addition, analysis of microbial community structure and taxonomic composition demonstrated that the deep subseafloor biosphere can be categorized into two types: the aerobic microbial ecosystem in the open ocean and the anaerobic microbial ecosystem in the marginal ocean, which populations are persisting vertically into deeper sedimentary habitats.

Research Products

• [Int'l Joint Research] ブレーメン大学/アルフレッドウェゲナー極地研究所/マックスプランク海洋微生物学研究所(ドイツ)
• [Int'l Joint Research] カリフォルニア工科大学/ロードアイランド大学/南カリフォルニア大学(米国)
• [Int'l Joint Research] チューリッヒ工科大学(スイス)
• [Int'l Joint Research] オーフス大学(デンマーク)
• [Int'l Joint Research] ブレーメン大学/マックスプランク海洋微生物学研究所/アルフレッドウェゲナー極地研究所(ドイツ)
• [Int'l Joint Research] カリフォルニア工科大学/ロードアイランド大学/クレイグベンター研究所(米国)
• [Int'l Joint Research] チューリッヒ工科大学(スイス)
• [Journal Article] Deep-biosphere methane production stimulated by geofluids in the Nankai accretionary complex. (2018)
  • Author(s): Ijiri, A., Inagaki, F., Kubo, Y., Adhikari, R. R., Hattori, S., Hoshino, et al.
  • Journal Title: Science Advances Volume: 印刷中
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] D:L-amino acid modeling reveals fast microbial turnover of days to months in the subsurface hydrothermal sediment of Guaymas Basin. (2018)
  • Author(s): Moller, M. H., Glombitza, C., Lever, M., Deng, L., Morono, Y., Inagaki, F., Doll, M., Su, C., Lomstein, B. A.
  • Journal Title: Frontiers in Microbiology Volume: 9 Pages: 967-967
  • DOI: 10.3389/fmicb.2018.00967
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 海底下の微生物起源ガスと生命活動との関わりー海洋科学掘削の最前線ー (2018)
  • Author(s): 稲垣 史生, 井尻 暁, 北田数也, 町山栄章
  • Journal Title: 石油技術協会誌 Volume: 83 Pages: 130-137
  • NAID: 130007966758
  • Peer Reviewed
• [Journal Article] Assessment for capacity to capture DNA aerosols by clean filters for molecular biology experiments. (2018)
  • Author(s): Morono, Y., Hoshino, T., Terada, T., Suzuki, T., Sato, T., Yuasa, H., Kubota, Y., and Inagaki, F.
  • Journal Title: Microbes and Environments Volume: 印刷中
  • Peer Reviewed / Open Access
• [Journal Article] 海底下深部生命圏フロンティアの探究と将来展望. (2018)
  • Author(s): 稲垣史生, 諸野祐樹, 星野辰彦, 井尻暁, 肖楠, 鈴木志野, 石井俊一, 浦本豪一郎, 寺田武志, 井町寛之, 久保雄介.
  • Journal Title: 地質学雑誌 Volume: 124 Pages: 77-92
  • NAID: 130007377667
  • Peer Reviewed / Open Access
• [Journal Article] Temperature Limit of the Deep Biosphere off Muroto. (2018)
  • Author(s): Heuer, V. B., Inagaki, F., Morono, Y., Kubo, Y., Maeda, L., and the Expedition 370 Scientists.
  • Journal Title: Proceedings of the International Ocean Discovery Program. Volume: 370 Pages: 1-50
  • DOI: 10.14379/iodp.proc.370.2017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Aerobic and Anaerobic Methanotrophic Communities Associated with Methane Hydrates Exposed on the Seafloor: A High-Pressure Sampling and Stable Isotope-Incubation Experiment (2017)
  • Author(s): Case, D., Ijiri, A., Morono, Y., Tavormina, P., Orphan V. J., and Inagaki, F.
  • Journal Title: Frontiers in Microbiology Volume: 8 Pages: 2569-2569
  • DOI: 10.3389/fmicb.2017.02569
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Methyl-compound use and slow growth characterize microbial life in 2-km-deep subseafloor coal and shale beds (2017)
  • Author(s): Trembath-Reichert, E., Morono, Y., Ijiri, A., Hoshino, T., Dowson, K. S., Inagaki, F., and Orphan, V. J.
  • Journal Title: Proc. Natl. Acad. Sci. U.S.A. Volume: 114 Issue: 44
  • DOI: 10.1073/pnas.1707525114
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Unusual metabolic diversity of hyperalkaliphilic microbial communities associated with subterranean serpentinization at The Cedars (2017)
  • Author(s): Suzuki Shino、Ishii Shun'ichi、Hoshino Tatsuhiko、Rietze Amanda、Tenney Aaron、Morrill Penny L、Inagaki Fumio、Kuenen J Gijs、Nealson Kenneth H
  • Journal Title: The ISME Journal Volume: 11 Issue: 11 Pages: 2584-2598
  • DOI: 10.1038/ismej.2017.111
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Estimation of the influence of sequencing errors and distribution of random-sequence tags on quantitative sequencing (2017)
  • Author(s): Tatsuhiko Hoshino, Yohei Hamada
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 印刷中 Issue: 3 Pages: 359-364
  • DOI: 10.1016/j.jbiosc.2017.04.003
  • NAID: 40021323754
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Atribacteria from the Subseafloor Sedimentary Biosphere Disperse to the Hydrosphere through Submarine Mud Volcanoes (2017)
  • Author(s): Hoshino, T., Toki, T., Ijiri, A., Morono, Y., Machiyama, H., Ashi, J., Okamura, K., and Inagaki, F.
  • Journal Title: Frontiers in Microbiology Volume: 8 Pages: 1135-1135
  • DOI: 10.3389/fmicb.2017.01135
  • NAID: 120006383302
  • Peer Reviewed / Open Access
• [Journal Article] Bioturbation as a key driver behind the dominance of Bacteria over Archaea in near-surface sediment (2017)
  • Author(s): Chen, X., Andersen, T. J., Morono, Y., Inagaki, F., Jorgensen, B. B., and Lever. M. A.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 2400-2400
  • DOI: 10.1038/s41598-017-02295-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Distribution of anaerobic carbon monoxide dehydrogenase genes in deep subseafloor sediments (2017)
  • Author(s): Tatsuhiko Hoshino, Fumio Inagaki
  • Journal Title: Letters in Applied Microbiology Volume: 64 Issue: 5 Pages: 355-363
  • DOI: 10.1111/lam.12727
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Exploration of cultivable fungal communities in deep coal‐bearing sediments from ~1.3 to 2.5 km below the ocean floor. (2017)
  • Author(s): Liu, C.-H., Huang, X., Xie, T.-N., Duan, N., Xue, Y.-R., Zhao, T.-X., Lever, M. A., Hinrichs, K.-U., and Inagaki, F.
  • Journal Title: Environmental Microbiology Volume: 19 Issue: 2 Pages: 803-818
  • DOI: 10.1111/1462-2920.13653
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] International Ocean Discovery Program Expedition 370 Preliminary Report: Temperature Limit of the Deep Biosphere off Muroto (2017)
  • Author(s): Verena B. Heuer, Fumio Inagaki, Yuki Morono, Yusuke Kubo, Lena Maeda, and Expedition 370 Scientists
  • Journal Title: International Ocean Discovery Program Volume: ** Pages: 1-29
  • DOI: 10.14379/iodp.pr.370.2017
  • Open Access / Int'l Joint Research
• [Journal Article] Predominance of Viable Spore-Forming Piezophilic Bacteria in High-Pressure Enrichment Cultures from ~1.5 to 2.4 km-Deep Coal-Bearing Sediments below the Ocean Floor (2017)
  • Author(s): Jiasong Fang、加藤千明、Gabriella Runko、能木 裕一、堀 知行、Jiangtao Li、諸野祐樹、稲垣史生
  • Journal Title: Frontiers in Microbiology Volume: 8 (137) Pages: 1-10
  • DOI: 10.3389/fmicb.2017.00137
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Application of Stochastic Labeling with Random-Sequence Barcodes for Simultaneous Quantification and Sequencing of Environmental 16S rRNA genes (2017)
  • Author(s): Tatsuhiko Hoshino, Fumio Inagaki
  • Journal Title: PLoS One Volume: 12 Issue: 1 Pages: e0169431-e0169431
  • DOI: 10.1371/journal.pone.0169431
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Variance and potential niche separation of microbial communities in subseafloor sediments off Shimokita Peninsula, Japan (2016)
  • Author(s): Takuro Nunoura, Yoshihiro Takaki, Shigeru Shimamura, Jungo Kakuta, Hiromi Kazama, Miho Hirai, Noriaki Masui, Hitoshi Tomaru, Yuki Morono, Hiroyuki Imachi, Fumio Inagaki, Ken Takai
  • Journal Title: Environmental Microbiology Volume: 18 Issue: 6 Pages: 1-16
  • DOI: 10.1111/1462-2920.13096
  • Peer Reviewed
• [Journal Article] Thermal properties and thermal structure in the deep-water coalbed basin off the Shimokita Peninsula, Japan (2016)
  • Author(s): Wataru Tanikawa, Osamu Tadai, Sumito Morita, Weiren Lin, Yasuhiro Yamada, Yoshinori Sanada, Kyaw Moe, Yu'suke Kubo, Fumio Inagaki
  • Journal Title: Marine and Petroleum Geology Volume: 73 Pages: 445-461
  • DOI: 10.1016/j.marpetgeo.2016.03.006
  • Peer Reviewed / Open Access
• [Journal Article] 海底下生命圏：生命存在の限界に挑む (2016)
  • Author(s): 稲垣史生
  • Journal Title: 現代化学, 東京化学同人 Volume: 538 Pages: 31-33
• [Journal Article] 海洋掘削科学：地球内部の生命圏フロンティアと生命生息限界に挑む (2016)
  • Author(s): 稲垣史生
  • Journal Title: JGL日本地球惑星科学連合ニュースレター Volume: 12 Pages: 8-10
  • Peer Reviewed
• [Journal Article] 地球に残された未知生命圏フロンティアに挑む―八戸沖から見る地球環境とエネルギーの未来― (2016)
  • Author(s): 稲垣史生
  • Journal Title: 青森県工業技術教育振興会会報 Volume: 28 Pages: 3-5
• [Journal Article] 海底下の微生物生態系と新規木質バイオマス代謝機能の探索 (2016)
  • Author(s): 稲垣史生, 大田ゆかり
  • Journal Title: 四国紙パルプ工業協会ニュースレター Volume: 41 Pages: 1-10
• [Journal Article] Phylogenetic Diversity of <i>aprA</i> Genes in Subseafloor Sediments on the Northwestern Pacific Margin off Japan (2015)
  • Author(s): Masataka Aoki, Ryota Kakiuchi, Takashi Yamaguchi, Ken Takai, Fumio Inagaki, Hiroyuki Imachi
  • Journal Title: Microbes and Environments Volume: 30 Issue: 3 Pages: 276-280
  • DOI: 10.1264/jsme2.ME15023
  • NAID: 130005099202
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access
• [Journal Article] Exploring deep microbial life in coal-bearing sediments down to ~2.5km below the seafloor (2015)
  • Author(s): INAGAKI FUMIO, Kai-Uwe Hinrichs,KUBO YUSUKE,Marshall W. Bowles,Verena B. Heuer,Wei-Li Hong,HOSHINO TATSUHIKO,IJIRI AKIRA,IMACHI HIROYUKI,ITO MOTOO,KANEKO MASANORI,Mark A. Lever,Lin Yu-Shin,Barbara A. Methe,Morita Sumito,MORONO YUKI,et al.
  • Journal Title: SCIENCE/AAAS/ Volume: 349 Issue: 6246 Pages: 420-424
  • DOI: 10.1126/science.aaa6882
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ecophysiology of Zetaproteobacteria Associated with Shallow Hydrothermal Iron-Oxyhydroxide Deposits in Nagahama Bay of Satsuma Iwo-Jima, Japan (2015)
  • Author(s): HOSHINO TATSUHIKO, Kuratomi Takashi,MORONO YUKI,Hori Tomoyuki,Oiwane Hisashi,Kiyokawa Shoichi,INAGAKI FUMIO
  • Journal Title: Frontiers in Microbiology Volume: 6 Pages: 1554-1554
  • DOI: 10.3389/fmicb.2015.01554
  • Peer Reviewed / Open Access
• [Journal Article] Presence of oxygen and aerobic communities from sea floor to basement in deep-sea sediments (2015)
  • Author(s): D’Hondt, S., Inagaki, F., Zarikian, C.A., Yamaguchi, Y.T., et al.
  • Journal Title: Nature Geoscience Volume: 8 Issue: 4 Pages: 299-304
  • DOI: 10.1038/ngeo2387
  • Peer Reviewed
• [Journal Article] Low frequency of endospore-specific genes in subseafloor sedimentary metagenomes (2015)
  • Author(s): Mikihiko Kawai, Ikuo Uchiyama, Hideto Takami, Fumio Inagaki
  • Journal Title: Environmental Microbiology Reports Volume: 7 Issue: 2 Pages: 341-350
  • DOI: 10.1111/1758-2229.12254
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Intact preservation of environmental samples by freezing under an alternating magnetic field (2015)
  • Author(s): Morono, Y., Terada, T., Yamamoto, Y., Xiao, N., Hirose, T., Sugeno, M., Ohwada, N., Inagaki F
  • Journal Title: Environ. Microbiol. Rep. Volume: 7 Issue: 2 Pages: 243-251
  • DOI: 10.1111/1758-2229.12238
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Origins of lithium in submarine mud volcano fluid in the Nankai accretionary wedge (2015)
  • Author(s): Nishio, Y., Ijiri, A., Toki, T., Morono, Y., Tanimizu, M., Nagaishi, K., and Inagaki, F.
  • Journal Title: Earth Plant. Sci. Lett. Volume: 414 Pages: 144-155
  • DOI: 10.1016/j.epsl.2015.01.018
  • Peer Reviewed
• [Journal Article] Cross-species, amplifiable microsatellite markers for neoverrucid barnacles from deep-sea hydrothermal vents developed using next-generation sequencing. (2014)
  • Author(s): Nakajima Y, Shinzato C, Khalturina M, Watanabe H, Inagaki F, Satoh N, Mitarai S.
  • Journal Title: Int. J. Mol. Sci. Volume: 15 Issue: 8 Pages: 14364-71
  • DOI: 10.3390/ijms150814364
  • Peer Reviewed / Open Access
• [Journal Article] An improved sample preparation method for imaging microstructures of fine-grained marine sediment using microfocus X-ray computed tomography and scanning electron microscopy (2014)
  • Author(s): Go-Ichiro Uramoto, Yuki Morono, Katsuyuki Uematsu, Fumio Inagaki
  • Journal Title: Limnology and Oceanography: Methods Volume: 12 Issue: 7 Pages: 469-483
  • DOI: 10.4319/lom.2014.12.469
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A new hybrid pressure-coring system for the drilling vessel Chikyu (2014)
  • Author(s): Y Kubo, Y Mizuguchi, F Inagaki, K Yamamoto
  • Journal Title: Scientific Drilling Volume: 17 Pages: 37-43
  • DOI: 10.5194/sd-17-37-2014
  • Peer Reviewed
• [Journal Article] Gold-ISH: A nano-size gold particle-based phylogenetic identification compatible with NanoSIMS. (2014)
  • Author(s): Kubota, K., Morono, Y. , Ito, M., Terada, T., Itezono, S., Harada, H., and Inagaki, F.
  • Journal Title: Syst. Appl. Microbiol. Volume: 37 Issue: 4 Pages: 261-266
  • DOI: 10.1016/j.syapm.2014.02.003
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Exploring multi-spheres interactions through scientific ocean drilling: A perspective from the deep biosphere. (2017)
  • Author(s): Fumio Inagaki , Kai-Uwe Hinrichs
  • Organizer: 日本地球惑星科学連合2017年大会
  • Int'l Joint Research / Invited
• [Presentation] Biosphere Frontiers: Global pictures and perspectives (2017)
  • Author(s): Fumio Inagaki
  • Organizer: Australasian IODP Regional Planning Workshop
  • Int'l Joint Research / Invited
• [Presentation] Deciphering geosphere-biosphere interactions and limits of life through scientific ocean drilling (2017)
  • Author(s): Fumio Inagaki
  • Organizer: Department Lecture, Department of Earth System Sciences, Yonsei University, Korea
  • Invited
• [Presentation] Metabolic diversity of hyperalkaliphilic microbial communities associated with serpentinization at The Cedars. (2017)
  • Author(s): Shino Suzuki, Shunichi Ishii, Tatsuhiko Hoshino, Fumio Inagaki, J. Gijs Kuenen, Kenneth H. Nealson
  • Organizer: Goldschmidt 2017
  • Int'l Joint Research / Invited
• [Presentation] Limits to deep subseafloor life and geosphere-biosphere interactions (2017)
  • Author(s): Fumio Inagaki, Steven D’Hondt, Kai-Uwe Hinrichs
  • Organizer: International Workshop on “Marine Geomicrobiology: A Matter of Energy"
  • Int'l Joint Research / Invited
• [Presentation] 海底下の微生物起源ガスと生命活動との関わり-海洋科学掘削の最前線- (2017)
  • Author(s): 稲垣史生
  • Organizer: 石油技術協会平成29年度秋季講演会
  • Invited
• [Presentation] High-pressure sampling and experimental tools for the deep-sea and subseafloor life study (2017)
  • Author(s): Fumio Inagaki
  • Organizer: Deep Carbon Observatory Cultivation Workshop 2017
  • Int'l Joint Research / Invited
• [Presentation] Deciphering geosphere-biosphere interactions and limits of life through scientific ocean drilling (2017)
  • Author(s): Fumio Inagaki, Kai-Uwe Hinrichs
  • Organizer: International Society for Subsurface Microbiology 2017
  • Int'l Joint Research / Invited
• [Presentation] 海底下生命圏における微生物生態系の機能と限界について (2017)
  • Author(s): 稲垣史生
  • Organizer: ComBio2017 生命科学系合同年次大会
  • Int'l Joint Research / Invited
• [Presentation] 海底下地下深く潜む微生物 (2017)
  • Author(s): 稲垣史生
  • Organizer: 第７回日本微生物学連盟フォーラム「微生物ー変わり者たちの素顔ー」
  • Invited
• [Presentation] 種子島沖海底泥火山群の間隙水の化学組成鉛直プロファイルから見積もられた泥火山の活動状況 (2017)
  • Author(s): 井尻暁、濱田洋平、土岐知弘、安慶名昂、星野辰彦、萩野恭子、町山栄章、芦寿一郎、稲垣史生
  • Organizer: 日本地球惑星科学連合2017年大会
  • Int'l Joint Research
• [Presentation] IODP Expedition 370: Temperature Limit of the Deep Biosphere off Muroto (2017)
  • Author(s): Yuki Morono, Verena B. Heuer, Fumio Inagaki, Yusuke Kubo, Lena Maeda, and the Expedition 370 Scientists
  • Organizer: 日本地球惑星科学連合2017年大会
  • Int'l Joint Research
• [Presentation] The global survey of subseafloor sedimentary microbiomes (2017)
  • Author(s): Tatuhiko Hoshino, Hideyuki Doi, Lars Wormer, Yuki Morono, Steven D'Hondt, Kai-Uwe Hinrichs, Fumio Inagaki
  • Organizer: Goldschmidt2017
  • Int'l Joint Research
• [Presentation] ランダムシーケンスタグを応用した定量シーケンス（qSeq）法の開発 (2017)
  • Author(s): 星野辰彦, 濱田洋平, 稲垣史生
  • Organizer: 環境微生物系学会合同大会2017
• [Presentation] 室戸沖海底下掘削による生命生息温度限界への挑戦（T-リミット） (2017)
  • Author(s): 諸野祐樹, Hauer B. Verena, 稲垣史生, 久保雄介, 前田玲奈, Expedition 370 Scientists
  • Organizer: 環境微生物系学会合同大会2017
• [Presentation] How stable iron isotopes can help identifying the limit of life in deep marine sediments of the Nankai Trough (IODP Expedition 370) (2017)
  • Author(s): Susann Henkel, Verena Heuer, Fumio Inagaki, Yuki Morono, Kai-Uwe Hinrichs and Expedition 370 Scientists
  • Organizer: International Workshop on “Marine Geomicrobiology: A Matter of Energy"
  • Int'l Joint Research
• [Presentation] Global microbial diversity in subseafloor sediment (2017)
  • Author(s): Tatsuhiko Hoshino, Hideyuki Doi, Lars Wormer, Yuki Morono, Kai-Uwe Hinrichs, Fumio Inagaki
  • Organizer: International Society for Subsurface Microbiology 2017
  • Int'l Joint Research
• [Presentation] Temperature Limit of the Deep Subseafloor Biosphere in the Nankai Trough Subduction Zone off Cape Muroto (IODP T-Limit Expedition 370) (2017)
  • Author(s): Yuki Morono, Verena B. Heuer, Fumio Inagaki, Yusuke Kubo, Lena Maeda, The Expedition 370 Scientists
  • Organizer: AGU Fall Meeting 2017
  • Int'l Joint Research
• [Presentation] Characterization of Deep Marine Subsurface Fungi from South Pacific Gyre Sediments (2017)
  • Author(s): Morgan Sobol, Mayra Rodriguez, Tatsuhiko Hoshino, Fumio Inagaki, Brandi Kiel Reese
  • Organizer: Astrobiology Graduate Conference 2017
  • Int'l Joint Research
• [Presentation] Secondary Methanogenesis in Dormant Submarine Mud Volcano off Tanegashima Island, Japan (2017)
  • Author(s): Ijiri A, Toki T, Agena K, Hoshino T, Hagino K, Hamada Y, Machiyama H, Ashi J, Inagaki F
  • Organizer: Goldschmidt 2017
  • Int'l Joint Research
• [Presentation] The deep biosphere in Bengal Fan sediments (IODP Exp. 354) (2017)
  • Author(s): Adhikari, R. R., Heuer, V. B., Elvert, M., Hoshino, T., Inagaki, F., Kallmeyer, J., Kitte, A., Womer, L., Hinrichs, K.-U.
  • Organizer: Goldschmidt 2017
  • Int'l Joint Research
• [Presentation] オープンクリーンシステムを用いた実験環境下におけるDNA汚染の影響評価 (2017)
  • Author(s): 寺田武志、諸野祐樹、星野辰彦、鈴木剛人、佐藤卓広、湯浅久史、久保田裕仁、稲垣史生
  • Organizer: 環境微生物系学会合同大会2017
• [Presentation] 生物電気化学や地球生命工学的手法による持続的CO2資源化・利活用システムの創出 (2016)
  • Author(s): 稲垣史生, 石井俊一, 井町寛之
  • Organizer: 日本化学会
  • Place of Presentation: 同志社大学、京都府京都市
  • Year and Date: 2016-03-24
  • Invited
• [Presentation] Exploring deep-biosphere frontiers through scientific ocean drilling (2016)
  • Author(s): Fumio Inagaki
  • Organizer: The Chikyu IODP Board Meeting in 2016
  • Place of Presentation: 神戸大学、兵庫県神戸市
  • Year and Date: 2016-03-23
  • Int'l Joint Research / Invited
• [Presentation] The deep mud-volcano biosphere associated with the Nankai accretionary complex: Implication for geosphere-biosphere interactions (2016)
  • Author(s): Fumio Inagaki
  • Organizer: Gordon Research Conference on Gas Hydrates
  • Place of Presentation: ガルベストン、アメリカ合衆国
  • Year and Date: 2016-03-04
  • Int'l Joint Research / Invited
• [Presentation] Exploring Frontiers of the Deep Biosphere through Scientific Ocean Drilling (2015)
  • Author(s): Fumio Inagaki
  • Organizer: University of Southern California Geobiology Course
  • Place of Presentation: ロサンゼルス、アメリカ合衆国
  • Year and Date: 2015-12-23
  • Int'l Joint Research / Invited
• [Presentation] Exploring Frontiers of the Deep Biosphere through Scientific Ocean Drilling (2015)
  • Author(s): Fumio Inagaki
  • Organizer: American Geophysical Union (AGU) 2015 Fall Meeting
  • Place of Presentation: サンフランシスコ、アメリカ合衆国
  • Year and Date: 2015-12-14
  • Int'l Joint Research / Invited
• [Presentation] 地球普遍物質の利活用による生物電気化学的バイオ資源の創出について (2015)
  • Author(s): 稲垣史生
  • Organizer: 平成28年度戦略目標の策定等に関するワークショップ「エネルギー・資源生産のためのバイ オ資源の利活用技術」
  • Place of Presentation: 文部科学省、東京都千代田区
  • Year and Date: 2015-11-13
  • Invited
• [Presentation] 地球に残された未知生命圏フロンティアに挑むー見えてきた生命圏と非生命圏のあいだー (2015)
  • Author(s): 稲垣史生
  • Organizer: 「ちきゅう」就航10周年記念シンポジウム
  • Place of Presentation: イイノホール、東京千代田区
  • Year and Date: 2015-11-12
  • Invited
• [Presentation] 八戸沖に拡がる海底下の森ー「ちきゅう」による世界最深の海底下生命圏の発見についてー (2015)
  • Author(s): 稲垣史生
  • Organizer: 青森県八戸市：地球深部探査船「ちきゅう」10周年記念シンポジウム
  • Place of Presentation: 八戸グランドホテル、青森県八戸市
  • Year and Date: 2015-10-30
  • Invited
• [Presentation] 海洋・海底下環境における炭素・エネルギー循環と革新的CO2資源化・利活用技術開発 (2015)
  • Author(s): 稲垣史生
  • Organizer: JST-CRDS 俯瞰とスコープ抽出のための検討会「エネルギーとバイオ（生物）」
  • Place of Presentation: TKP市ヶ谷カンファレンスセンター、東京都新宿区
  • Year and Date: 2015-10-15
  • Invited
• [Presentation] 海底下深部の非在来型炭化水素資源環境における生物地球化学的炭素循環とその環境規定要因 (2015)
  • Author(s): 稲垣史生、Kai-Uwe Hinrichs、IODP Expedition 337 Scientists
  • Organizer: 日本地球化学会
  • Place of Presentation: 横浜国立大学、神奈川県横浜市
  • Year and Date: 2015-09-18
  • Invited
• [Presentation] 海底泥火山深部生命圏の実態解明にむけて (2015)
  • Author(s): 稲垣史生、井尻暁
  • Organizer: 日本地質学会
  • Place of Presentation: 信州大学、長野県長野市
  • Year and Date: 2015-09-13
  • Invited
• [Presentation] Exploring the extent and limits of the deep biosphere associated with coal-bearing hydrocarbon system down to ~2.5 km below the seafloor off Shimokita, Japan (2015)
  • Author(s): Fumio Inagaki, Kai-Uwe Hinrichs, IODP Expedition 337 Scientists
  • Organizer: The 3rd International Symposium on Application of Nano-Geosciences in Petroleum Engineering
  • Place of Presentation: 京都文化プラザ、京都府京都市
  • Year and Date: 2015-09-05
  • Int'l Joint Research / Invited
• [Presentation] Application of NanoSIMS to Deep Subseafloor Life (2015)
  • Author(s): Fumio Inagaki
  • Organizer: SISS-17: The Scientific International Symposium on SIMS and Related Techniques Based on Ion-Solid Interactions
  • Place of Presentation: 成蹊大学、東京都武蔵野市
  • Year and Date: 2015-06-26
  • Int'l Joint Research / Invited
• [Presentation] 海底下の生命活動と炭素循環の脈動ー持続的な炭素・エネルギー循環システムの構築に向けて (2015)
  • Author(s): 稲垣史生
  • Organizer: 石油資源開発株式会社セミナー
  • Place of Presentation: 石油資源開発株式会社東京本社、東京都千代田区
  • Year and Date: 2015-06-25
  • Invited
• [Presentation] 海洋科学技術が切り拓く地球環境とエネルギーの未来 (2015)
  • Author(s): 稲垣史生
  • Organizer: 第三回シンポジウム「海洋科学技術が切り拓く地球環境とエネルギーの未来ーCO2回収・利活用技術 のインパクトー」
  • Place of Presentation: 一橋講堂、東京都国立市
  • Year and Date: 2015-06-24
  • Invited
• [Presentation] 海底下の生命活動と炭素循環研究から未来を探る (2015)
  • Author(s): 稲垣史生
  • Organizer: 海洋研究開発機構科学メディア意見交換会「地球掘削科学の今までそしてこれから」
  • Place of Presentation: 海洋研究開発機構東京事務所、東京都千代田区
  • Year and Date: 2015-06-15
• [Presentation] 科学海洋掘削による海底下限界生命圏の追究 (2015)
  • Author(s): 稲垣史生
  • Organizer: 日本地球惑星連合大会
  • Place of Presentation: 幕張メッセ国際会議場、千葉県千葉市
  • Year and Date: 2015-05-27
  • Invited
• [Presentation] Limits and habitability of microbial life in the deep subseafloor biosphere (2015)
  • Author(s): Fumio Inagaki, Kai-Uwe Hinrichs, Steven D'Hondt
  • Organizer: International Marine Microbiology Conference
  • Place of Presentation: 青島、中国
  • Year and Date: 2015-05-24
  • Int'l Joint Research / Invited
• [Presentation] Exploring deep life in coal-bearing sediments down to ~2.5km below the seafloor (2015)
  • Author(s): Fumio Inagaki, Kai-Uwe Hinrichs, IODP Expedition 337 Scientists
  • Organizer: MPI Seminar
  • Place of Presentation: ブレーメン、ドイツ
  • Year and Date: 2015-05-12
  • Int'l Joint Research / Invited
• [Presentation] Exploring deep microbial life in coal-bearing sediments down to ~2.5km below the seafloor (2015)
  • Author(s): Fumio Inagaki, Kai-Uwe Hinrichs, IODP Expedition 337 Scientists
  • Organizer: International Symposium for Frontiers in Marine Geology-Deep Life and Deep Carbon
  • Place of Presentation: 高雄、台湾
  • Year and Date: 2015-04-16
  • Int'l Joint Research / Invited
• [Presentation] Constraining the temperature limit of the microbial deep biosphere in the Nankai Trough subseafloor (2015)
  • Author(s): Fumio Inagaki, Kai-Uwe Hinrichs, Verena Heuer
  • Organizer: International Symposium for Frontiers in Marine Geology-Deep Life and Deep Carbon
  • Place of Presentation: 高雄、台湾
  • Year and Date: 2015-04-16
  • Int'l Joint Research / Invited
• [Presentation] Exploring deep life in coal-bearing sediments down to ~2.5km below the seafloor (2015)
  • Author(s): Fumio Inagaki, Kai-Uwe Hinrichs and IODP Expedition 337 shipboard and shore-based scientists
  • Organizer: Deep Carbon Observatory (DCO) 2nd International Science Meeting
  • Place of Presentation: ミュンヘン、ドイツ
  • Year and Date: 2015-03-26 – 2015-03-28
  • Invited
• [Presentation] 地球深部探査船「ちきゅう」による海底下生命圏の探求 ー海底下の森に生命を探るー (2015)
  • Author(s): 稲垣史生
  • Organizer: 四国紙パルプ研究協議会設立40執念記念講演会
  • Place of Presentation: 高知大学農学部、高知県南国市
  • Year and Date: 2015-03-10
  • Invited
• [Presentation] IODP Expedition 337 Deep coalbed biosphere off Shimokita (2014)
  • Author(s): Fumio Inagaki, Kai-Uwe Hinrichs
  • Organizer: ECORD Summer School 2014
  • Place of Presentation: ブレーメン、ドイツ
  • Year and Date: 2014-10-22 – 2014-10-26
  • Invited
• [Presentation] Methodological advances for the identification of subseafloor communities and their activities (2014)
  • Author(s): Fumio Inagaki, Yuki Morono, Motoo Ito, Tatsuhiko Hoshino
  • Organizer: ECORD Summer School 2014
  • Place of Presentation: ブレーメン、ドイツ
  • Year and Date: 2014-10-22 – 2014-10-26
  • Invited
• [Presentation] 海底下生命圏の地球横断的解析ー存在量・多様性を規定する因子の解明ー (2014)
  • Author(s): 星野辰彦，諸野祐樹，寺田武志，稲垣史生
  • Organizer: 環境微生物系学会合同大会2014
  • Place of Presentation: 浜松アクトシティー、静岡県浜松市
  • Year and Date: 2014-10-17 – 2014-10-20
• [Presentation] 科学海洋掘削による地球深部生命の探究 (2014)
  • Author(s): 稲垣史生
  • Organizer: 高知大学コア保管庫落成記念シンポジウム
  • Place of Presentation: 高知大学海洋コア総合研究センター、高知県南国市
  • Year and Date: 2014-10-17
  • Invited
• [Presentation] Global Subseafloor Microbiomes Project (2014)
  • Author(s): Tatsuhiko Hoshino, Yuki Morono, and Fumio Inagaki
  • Organizer: International Symposium for Subsurface Microbiology (ISSM) 2014
  • Place of Presentation: ソウル、韓国
  • Year and Date: 2014-10-05 – 2014-10-10
• [Presentation] The deep mud-volcano biosphere in the Nankai Trough accretionary complex (2014)
  • Author(s): Fumio Inagaki, Akira Ijiri, Yuki Morono, Tatsuhiko Hoshino, and Expeditions 903 and 906 Scientists
  • Organizer: International Symposium for Subsurface Microbiology (ISSM) 2014
  • Place of Presentation: モントレー、米国
  • Year and Date: 2014-10-05 – 2014-10-10
• [Presentation] Drilling technology and contamination assessment for deep and low-biomass samples (2014)
  • Author(s): Fumio Inagaki, Kai-Uwe Hinrichs, Yusuke Kubo
  • Organizer: Deep Carbon Observatory (DCO) Deep Life Directory Meeting
  • Place of Presentation: モントレー、米国
  • Year and Date: 2014-10-02 – 2014-10-05
  • Invited
• [Presentation] Limits and habitability of subseafloor life in the deep sedimentary biosphere (2014)
  • Author(s): Fumio Inagaki, Kai-Uwe Hinrichs, Steven D'Hondt
  • Organizer: International Society for Microbial Ecology (ISME) 2014
  • Place of Presentation: ソウル、韓国
  • Year and Date: 2014-09-24 – 2014-09-29
  • Invited
• [Presentation] Limits and habitability of subseafloor life in the deep sedimentary biosphere (2014)
  • Author(s): Fumio Inagaki, Kai-Uwe Hinrichs, Steven D'Hondt
  • Organizer: 中国海洋大学
  • Place of Presentation: 青島、中国
  • Year and Date: 2014-07-23
  • Invited
• [Presentation] Limits and habitability of subseafloor life in the deep sedimentary biosphere (2014)
  • Author(s): Fumio Inagaki, Kai-Uwe Hinrichs, Steven D'Hondt
  • Organizer: 上海交通大学
  • Place of Presentation: 上海、中国
  • Year and Date: 2014-07-20
  • Invited
• [Presentation] 日本近海の海底炭化水素資源と炭素循環 (2014)
  • Author(s): 稲垣史生
  • Organizer: 高知市市民大学
  • Place of Presentation: 高知カルポートプラザ、高知県高知市
  • Year and Date: 2014-07-15
  • Invited
• [Book] Developments in Marine Geology volume 7, Earth and Life Processes Discovered from Subseafloor Environments, (eds.) R. Stein, D. Blackman, F. Inagaki, and H.C. Larsen (2014)
  • Author(s): Fumio Inagaki and Victoria Orphan
  • Total Pages: 829
  • Publisher: Elsevier
• [Patent(Industrial Property Rights)] 標的拡散の定量方法及びそのためのキット (2017)
  • Inventor(s): 星野辰彦, 稲垣史生
  • Industrial Property Rights Holder: 星野辰彦, 稲垣史生
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017
  • Overseas
• [Patent(Industrial Property Rights)] 標的核酸の定量方法及びそのためのキット (2015)
  • Inventor(s): 星野辰彦, 稲垣史生
  • Industrial Property Rights Holder: 星野辰彦, 稲垣史生
  • Industrial Property Rights Type: 特許
  • Filing Date: 2015-11-18