| Budget Amount *help |
¥41,860,000 (Direct Cost: ¥32,200,000、Indirect Cost: ¥9,660,000)
Fiscal Year 2016: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2014: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
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| Outline of Final Research Achievements |
To elucidate biogeographical distribution of deep microbial life in the global subseafloor sedimentary environment which accounts for ~70% of the Earth's surface, we extracted environmental genomic DNA from frozen core samples obtained by multiple scientific ocean drilling expeditions in the past decade and performed the comprehensive quantification and sequence analysis of 16S rRNA genes. Consequently, the proportion of Archaea in the global subseafloor sedimentary biosphere was estimated at ~37%, which is nearly consistent with archaeal abundance in the overlying marine environment. In addition, analysis of microbial community structure and taxonomic composition demonstrated that the deep subseafloor biosphere can be categorized into two types: the aerobic microbial ecosystem in the open ocean and the anaerobic microbial ecosystem in the marginal ocean, which populations are persisting vertically into deeper sedimentary habitats.
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