| Budget Amount *help |
¥39,910,000 (Direct Cost: ¥30,700,000、Indirect Cost: ¥9,210,000)
Fiscal Year 2017: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2016: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2015: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2014: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
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| Outline of Final Research Achievements |
Seven proteins located in mitochondria were selected from 13 candidate proteins from AmBP30 protein complex responsible for mRNA processing of ACR-toxin receptor gene ACRS. AmBP30 protein which binds to ACRS mRNA, was localized in mitochondria only from ACR-toxin insensitive citrus cultivars. ACRTS1, ACRTS2, ACRTS3, and ACRTS4 were isolated as cluster genes for ACT-toxin biosynthesis enzymes and their functions were analyzed. Immunoprecipitation using anti-ACTT1 antibodies identified all 9 enzymes which had been known to have a responsibility for ACT-toxin biosynthesis, as well as additional new 7 candidate enzymes and their genes for the toxin biosynthesis.
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