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Molecular basis of the complex morphogenesis of a rare actinomycete

Research Project

Project/Area Number 26252010
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionThe University of Tokyo

Principal Investigator

Ohnishi Yasuo  東京大学, 大学院農学生命科学研究科(農学部), 教授 (90292789)

Co-Investigator(Renkei-kenkyūsha) Takeaki Tezuka  東京大学, 大学院農学生命科学研究科, 助教 (80646414)
Project Period (FY) 2014-06-27 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥40,170,000 (Direct Cost: ¥30,900,000、Indirect Cost: ¥9,270,000)
Fiscal Year 2016: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2015: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2014: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Keywords放線菌 / 希少放線菌 / 形態分化 / 遺伝子発現制御 / 胞子嚢 / べん毛 / 転写因子 / 菌糸 / ChIP-seq / mRNA-seq / 微生物 / 運動性胞子
Outline of Final Research Achievements

The rare actinomycete Actinoplanes missouriensis shows complex morphological differentiation. A. missouriensis is a filamentous bacterium and produces terminal sporangia that include hundreds of flagellated spores. Upon contact with water, the spores are released from the sporangia by dehiscence and swim rapidly for a short period of time until they find niches for germination. In this research project, I studied the molecular basis of its complex morphogenesis by several approaches and obtained new knowledge of the fundamental issues of cell biology, such as “sleeping and awakening of cells” and “regulation of cell motility”.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (26 results)

All 2017 2016 2015 2014

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 3 results) Presentation (23 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Journal Article] Regulation of sporangium formation by BldD in the rare actinomycete Actinoplanes missouriensis2017

    • Author(s)
      Yoshihiro Mouri, Kenji Konishi, Azusa Fujita, Takeaki Tezuka, Yasuo Ohnishi
    • Journal Title

      Journal of Bacteriology

      Volume: 199 Issue: 12

    • DOI

      10.1128/jb.00840-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Genetic and Transcriptional Analyses of the Flagellar Gene Cluster in Actinoplanes missouriensis2016

    • Author(s)
      Moon-Sun Jang, Yoshihiro Mouri, Kaoru Uchida, Shin-Ichi Aizawa, Masayuki Hayakawa, Nobuyuki Fujita, Takeaki Tezuka and Yasuo Ohnishi
    • Journal Title

      Journal of Bacteriology

      Volume: 198 Issue: 16 Pages: 2219-2227

    • DOI

      10.1128/jb.00306-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Isolation of a novel plasmid from Couchioplanes caeruleus and construction of two plasmid vectors for gene expression in Actinoplanes missouriensis2015

    • Author(s)
      Moon-Sun Jang, Azusa Fujita, Satomi Ikawa, Keitaro Hanawa, Hideki Yamamura, Tomohiko Tamura, Masayuki Hayakawa, Takeaki Tezuka, Yasuo Ohnishi
    • Journal Title

      Plasmid

      Volume: 77 Pages: 32-38

    • DOI

      10.1016/j.plasmid.2014.12.001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 希少放線菌Actinoplanes missouriensisの胞子嚢形成に関与するAMIS76070と相互作用するタンパク質の探索2017

    • Author(s)
      手塚武揚、小山達樹、安久都卓哉、大西康夫
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都府・京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 希少放線菌Actinoplanes missouriensisのべん毛形成に必須なチオレドキシンAMIS75470の機能部位および標的タンパク質の同定2017

    • Author(s)
      木村知宏、手塚武揚、大西康夫
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都府・京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] AMIS75470 is Essential for Flagellar Assembly in the Rare Actinomycete Actinoplanes missouriensis2017

    • Author(s)
      Tomohiro Kimura, Takeaki Tezuka, Shin-Ichi Aizawa, Yasuo Ohnishi
    • Organizer
      The OIST International Workshop on Bacterial Flagella, Injectisome and Type III Secretion System
    • Place of Presentation
      沖縄科学技術大学院大学(沖縄県・国頭郡恩納村)
    • Year and Date
      2017-03-01
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of an essential gene for flagella synthesis in Actinoplanes missouriensis2016

    • Author(s)
      Tomohiro kimura, Takeaki Tezuka, Yasuo Ohnishi
    • Organizer
      The 13th International Symposium on the Genetics of Industrial Microorganisms
    • Place of Presentation
      Wuhan, China
    • Year and Date
      2016-10-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Involvement of the sole SsgA-like protein (SsgB-Am) in the sporangium formation in Actinoplanes missouriensis2016

    • Author(s)
      Yasuo Ohnishi
    • Organizer
      The 13th International Symposium on the Genetics of Industrial Microorganisms
    • Place of Presentation
      Wuhan, China
    • Year and Date
      2016-10-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 希希少放線菌Actinoplanes missouriensisの形態分化を制御する転写制御因子BldDの機能解析2016

    • Author(s)
      毛利佳弘、小西健司、手塚武揚、大西康夫
    • Organizer
      グラム陽性菌ゲノム機能会議
    • Place of Presentation
      KKRホテル熱海(静岡県・熱海市)
    • Year and Date
      2016-08-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] 希少放線菌Actinoplanes missouriensisにおける胞子嚢形成の制御遺伝子AMIS_76070の機能解析2016

    • Author(s)
      安久都 卓哉、平田 愛子、手塚 武揚、大西 康夫
    • Organizer
      2016年度日本農芸化学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 希少放線菌Actinoplanes missouriensisの休眠を制御する転写制御因子TcrAのレギュロンの網羅的同定2016

    • Author(s)
      毛利 佳弘、手塚 武揚、大西 康夫
    • Organizer
      2016年度日本農芸化学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] dRNAシーケンスによる希少放線菌Actinoplanes missouriensis胞子嚢の開裂過程に働く遺伝子のプロモーター領域の解析2016

    • Author(s)
      安田 理沙、手塚 武揚、大西 康夫
    • Organizer
      2016年度日本農芸化学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 希少放線菌Actinoplanes missouriensisの遊走子べん毛回転停止に必須な遺伝子ftgAの機能解析2016

    • Author(s)
      木村 知宏、種村 裕幸、手塚 武揚、大西 康夫
    • Organizer
      2016年度日本農芸化学会大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] 希少放線菌Actinoplanes missouriensisの遊走子の走化性に必須なcheクラスターの機能解析2016

    • Author(s)
      木村 知宏、張 文スン、種村 裕幸、手塚 武揚、大西 康夫
    • Organizer
      第21回べん毛交流会
    • Place of Presentation
      山形県天童温泉 滝の湯(山形県・天童市)
    • Year and Date
      2016-03-06
    • Related Report
      2015 Annual Research Report
  • [Presentation] 希少放線菌の「休眠と覚醒」に関する遺伝子発現制御機構2015

    • Author(s)
      大西康夫
    • Organizer
      日本農芸化学会2015年度大会(シンポジウム)
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 希少放線菌Actinoplanes missouriensisにおける遊走子形成を制御する遺伝子ssgMの機能解析2015

    • Author(s)
      安久都卓哉、手塚武揚、大西康夫
    • Organizer
      日本農芸化学会2015年度大会(一般講演)
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 希少放線菌Actinoplanes missouriensisの遊走胞子べん毛の回転運動の解析2015

    • Author(s)
      手塚武揚、堺隆之、曽和義幸、大西康夫
    • Organizer
      日本農芸化学会2015年度大会(一般講演)
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 放線菌Actinoplanes missouriensisの胞子嚢開裂に必要な遺伝子のRNA-Seq解析による探索2015

    • Author(s)
      安田理沙、毛利佳弘、手塚武揚、大西康夫
    • Organizer
      日本農芸化学会2015年度大会(一般講演)
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 希少放線菌Actinoplanes missouriensisの運動性胞子の走化性制御メカニズムの解析2015

    • Author(s)
      木村知宏、手塚武揚、中村周吾、奥寛雅、大西康夫
    • Organizer
      日本農芸化学会2015年度大会(一般講演)
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 放線菌Actinoplanes missouriensisの胞子嚢の開裂に必要な遺伝子のRNA-Seqによる探索2015

    • Author(s)
      安田理沙、毛利佳弘、手塚武揚、大西康夫
    • Organizer
      第9回日本ゲノム微生物学会年会
    • Place of Presentation
      神戸大学
    • Year and Date
      2015-03-06 – 2015-03-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] BldDは希少放線菌Actinoplanes missouriensis の胞子嚢形成を制御する2015

    • Author(s)
      毛利佳弘、小西健司、手塚武揚、大西康夫
    • Organizer
      第9回日本ゲノム微生物学会年会
    • Place of Presentation
      神戸大学
    • Year and Date
      2015-03-06 – 2015-03-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] Morphological development of the rare actinomycete Actinoplanes missouriensis2014

    • Author(s)
      Yasuo Ohnishi
    • Organizer
      17th International Symposium on the Biology of Actinomycetes
    • Place of Presentation
      Kusadasi-Aydin, Turkey
    • Year and Date
      2014-10-08 – 2014-10-12
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Functional analysis of the response regulator TcrA involved in the flagellar synthesis and spore dormancy in Actinoplanes missouriensis2014

    • Author(s)
      Yoshihiro Mouri, Moon-Sun Jang, Kenji Konishi, Takeaki Tezuka, Yasuo Ohnishi
    • Organizer
      17th International Symposium on the Biology of Actinomycetes
    • Place of Presentation
      Kusadasi-Aydin, Turkey
    • Year and Date
      2014-10-08 – 2014-10-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] 希少放線菌Actinoplanes missouriensisの胞子嚢開裂メカニズムの解析2014

    • Author(s)
      手塚武揚、安田理沙、大西康夫
    • Organizer
      グラム陽性細菌のゲノム生物学研究会
    • Place of Presentation
      鶴岡
    • Year and Date
      2014-09-03 – 2014-09-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 希少放線菌Actinoplanes missouriensisのべん毛形成に必須な遺伝子AMIS75470に関する解析2014

    • Author(s)
      木村知宏、手塚武揚、大西康夫
    • Organizer
      第13回 微生物研究会
    • Place of Presentation
      東京農大
    • Year and Date
      2014-07-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 希少放線菌Actinoplanes missouriensisの胞子嚢開裂メカニズムの解析2014

    • Author(s)
      安田理沙、手塚武揚、大西康夫
    • Organizer
      第13回 微生物研究会
    • Place of Presentation
      東京農大
    • Year and Date
      2014-07-26
    • Related Report
      2014 Annual Research Report

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Published: 2014-11-20   Modified: 2018-03-22  

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