Autonomous regulatory mechanism of metal ion levels by membrane transport system and metal-binding protein

• Project/Area Number: 26252011
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Nagoya University
• Principal Investigator: Maeshima Masayoshi 名古屋大学, 生命農学研究科, 教授 (80181577)
• Co-Investigator(Renkei-kenkyūsha): KAWACHI Miki 名古屋大学, 大学院生命農学研究科, 准教授 (40625125)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥40,300,000 (Direct Cost: ¥31,000,000、Indirect Cost: ¥9,300,000); Fiscal Year 2016: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000); Fiscal Year 2015: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000); Fiscal Year 2014: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
• Keywords: Membrane / Metals / Membrane transpoter / Ion concentration / Membrane transporter / 液胞 / 金属イオン / 膜輸送 / 金属耐性 / 亜鉛輸送体 / 細胞膜 / 液胞膜 / 金属輸送体 / プロトンポンプ

Outline of Final Research Achievements

Plants regulate concentrations of metal ions in order to maintain their activities. We focused on the ion transport systems and ion-sensing machineries and revealed the followings. 1) The cytosolic histidine-rich loop changes its configuration and functions acts as a sensor of cytosolic zinc to maintain an essential level in the cytosol. This was evidence by in vivo examination using loss-of-function mutant plants. 2) New-type zinc transporter MTP12 forms a hetero-oligomer with MTP5 and functions as zinc transporter in the membrane of Golgi apparatus. 3) A newly identified calcium-binding protein PCaP1, which is stably associated with the plasma membrane, changes its conformation by binding with metal ions. The expression of PCaP1 gene is enhanced by increased copper levels and its loss-of-function mutant plants become sensitive to high copper levels. Also, PCaP1 is involved in the closing mechanism of stomata in leaves.

Research Products

• [Int'l Joint Research] チューリッヒ大学(スイス)
• [Int'l Joint Research] ハイデルベルグ大学(ドイツ)
• [Int'l Joint Research] ポハン科学技術大学(韓国)
• [Journal Article] Compensated cell enlargement in fugu5 is specifically triggered by lowered sucrose production from seed storage lipids. (2017)
  • Author(s): Takahashi, K., Morimoto, R., Ishida, M., Asaoka, M., Maeshima, M., Tsukaya, H., and Ferjani, A.
  • Journal Title: Plant & Cell Physiology Volume: 58 Issue: 4 Pages: 668-678
  • DOI: 10.1093/pcp/pcx021
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A novel-type phosphatidylinositol phosphate-interactive, Ca-binding protein PCaP1 in Arabidopsis thaliana: stable association with plasma membrane and partial involvement in stomata closure. (2016)
  • Author(s): Nagata, C., Miwa, C., Tanaka, N., Kato, M., Suito, M., Tsuchihira, A., Sato, Y., Segami, S. and Maeshima, M.
  • Journal Title: Journal of Plant Research Volume: In press Issue: 3 Pages: 539-550
  • DOI: 10.1007/s10265-016-0787-2
  • NAID: 120005868252
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Contribution of PPi-hydrolyzing function of vacuolar H+-pyrophosphatase in vegetative growth of Arabidopsis: Evidenced by expression of uncoupling mutated enzymes. (2016)
  • Author(s): Asaoka, M., Segami, S., Ferjani, A., and Maeshima, M.
  • Journal Title: Frontiers in Plant Research Volume: 7 Pages: 415-415
  • DOI: 10.3389/fpls.2016.00415
  • NAID: 120005868253
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Lack of H+-pyrophosphatase causes developmental damage in Arabidopsis leaves in ammonia-free culture medium. (2016)
  • Author(s): Fukuda, M., Segami, S., Tomoyama, T., Nakanishi, Y., Gunji, S., Ferjani, A., and Maeshima, M.
  • Journal Title: Frontiers of Plant Science Volume: 6 Pages: 819-819
  • DOI: 10.3389/fpls.2016.00819
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Multiple facets of H+-pyrophosphatase and related enzymes. (2016)
  • Author(s): Ferjani, A., and Maeshima, M.
  • Journal Title: Frontiers of Plant Science Volume: 8
  • DOI: 10.3389/fpls.2016.01265
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The histidine-rich loop of Arabidopsis vacuolar membrane zinc transporter AtMTP1 is involved in sensing cytosolic level of zinc. (2015)
  • Author(s): Tanaka, N., Fujiwara, T., Tomioka, R., Krämer, U., Kawachi, M., and Maeshima, M.
  • Journal Title: Plant and Cell Physiology Volume: 56 Issue: 3 Pages: 510-519
  • DOI: 10.1093/pcp/pcu194
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of a unique CDF member containing the long N-terminal region in Arabidopsis thaliana. (2015)
  • Author(s): Fujiwara, T., Mori, H., Kawachi, M., and Maeshima, M.
  • Journal Title: FEBS Journal Volume: 282 Pages: 1965-1979
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Up-regulation of genes involved in N-acetylglucosamine uptake and metabolismsuggests a recycling mode of chitin in intraradical mycelium of arbuscular mycorrhizal fungi (2015)
  • Author(s): Yoshihiro Kobae, Miki Kawachi, Katsuharu Saito, Yusuke Kikuchi, Tatsuhiro Ezawa, Masayoshi Maeshima, Shingo Hata and Toru Fujiwara
  • Journal Title: Mycorrhiza Volume: 25 Issue: 5 Pages: 00-00
  • DOI: 10.1007/s00572-014-0623-2
  • NAID: 120005820694
  • Peer Reviewed / Open Access
• [Journal Article] GFPの二量体化を原因とする細胞内オルガネラの異常構造の形成とその予防策 (2015)
  • Author(s): 瀬上紹嗣、前島正義
  • Journal Title: 化学と生物 Volume: 53 Pages: 703-708
  • NAID: 130005416123
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characteristics of a root hair-less line of Arabidopsis thaliana under physiological stresses. (2014)
  • Author(s): Tanaka, N., Kato, M., Tomioka, R., Kurata, R., Fukao, Y., Aoyama, T., and Maeshima, M.
  • Journal Title: Journal of Experimental Botany Volume: 65 Issue: 6 Pages: 1497-1512
  • DOI: 10.1093/jxb/eru014
  • Peer Reviewed / Open Access
• [Journal Article] Accumulation of TIP2;2 aquaporin during dark adaptation is partially phyA dependent in roots of Arabidopsis seedlings. (2014)
  • Author(s): Uenishi, Y., Nakabayashi, Y., Tsuchihira, A., Takusagawa, M., Hashimoto, K., Maeshima M., and Sato-Nara, K.
  • Journal Title: Plants Volume: 3 Issue: 1 Pages: 177-195
  • DOI: 10.3390/plants3010177
  • Peer Reviewed
• [Journal Article] Roles of the vacuolar H+-PPase in seed storage oil mobilization and plant development. (2014)
  • Author(s): Ferjani, A., Segami, S., Horiguchi, G., Muto, Y., Maeshima, M., and Tsukaya, H.
  • Journal Title: Plant Morphology Volume: 26 Pages: 45-51
  • NAID: 130005066549
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Dynamics of vacuoles and H+-pyrophosphatase visualized by monomeric green fluorescent protein in Arabidopsis: Artifactual bulbs and native intravacuolar spherical structures. (2014)
  • Author(s): Segami, S., Makino, S., Miyake, A., Asaoka, M., and Maeshima, M.
  • Journal Title: Plant Cell Volume: 26 Issue: 8 Pages: 3416-3434
  • DOI: 10.1105/tpc.114.127571
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of the critical residues for the function of vacuolar H+-pyrophosphatase by amino acid screening based on the 3D structure. (2014)
  • Author(s): Asaoka, M., Segami, S., and Maeshima, M.
  • Journal Title: Journal of Biochemistry Volume: 156 Issue: 6 Pages: 333-344
  • DOI: 10.1093/jb/mvu046
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] (43) 河内美樹、藤原崇志、田中奈月、前島正義：植物の亜鉛膜輸送体からみた亜鉛ホメオスタシスと亜鉛シグナリング (2014)
  • Author(s): 河内美樹、藤原崇志、田中奈月、前島正義
  • Journal Title: 生化学 Volume: 86 Pages: 407-410
  • Peer Reviewed
• [Presentation] Multiple facets of vacuolar H+-pyrophosphatase and vacuolar functions: visible and invisible parts. (2017)
  • Author(s): Masayoshi MAESHIMA
  • Organizer: Annual Meeting of Japanese Society of Plant Physiologists. (Symposium)
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2017-03-16
  • Int'l Joint Research / Invited
• [Presentation] 植物の成長最適化に関わる代謝産物ピロリン酸濃度の調節. (2016)
  • Author(s): 前島正義、瀬上紹嗣、浅岡真理子、福田茉由、郡司 玄、Ali Ferjani
  • Organizer: 日本植物学会大会（シンポジウム）
  • Place of Presentation: Okinawa
  • Year and Date: 2016-09-16
  • Invited
• [Presentation] Dual functions of vacuolar H+-pyrophosphatase: vacuolar acidification and PPi homeostasis. (2016)
  • Author(s): Maeshima M, Segami S, Fukuda M, Asaoka M, Ferjani A
  • Organizer: 17th International Workshop on Plant Membrane Biology
  • Place of Presentation: Annapolis, Maryland, USA
  • Year and Date: 2016-06-05
  • Int'l Joint Research / Invited
• [Presentation] Cooperative works of H+-PPase and soluble PPases for PPi homeostasis in Arabidopsis. (2016)
  • Author(s): Segami, S., Tomoyama, T., Maeshima, M.
  • Organizer: 日本植物生理学会2016年度年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] Contribution of PPi-hydrolysis function of H+-pyrophosphatase in vegetative growth of Arabidopsis thaliana. (2016)
  • Author(s): Asaoka, M., Segami, S., Ferjani, A., Maeshima, M.
  • Organizer: 日本植物生理学会2016年度年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] Physiological effects of loss-of-function mutations of enzymes involved in pyrophosphate metabolism depending on nitrogen. (2016)
  • Author(s): Fukuda, M., Segami, S., Tomoyama, T., Gunji, S., Ferjanai, A., Maeshima, M.
  • Organizer: 日本植物生理学会2016年度年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] A Ca2+-binding protein PCaP1 is involved in root hydrotropism. (2016)
  • Author(s): Tanaka, N., Kobayashi, H., Takahashi, H., Maeshima, M.
  • Organizer: 日本植物生理学会2016年度年会
  • Place of Presentation: 岩手大学
  • Year and Date: 2016-03-18
• [Presentation] Characterization of the histidine loop of Arabidopsis vacuolar membrane zinc transporter AtMTP1 as a sensor of cytosol zinc level (2015)
  • Author(s): Tanaka, N., Fujiwara, T., Tomioka, R., Kraemer, U., Kawachi M. and Maeshima, M
  • Organizer: Vienna International Conference Series 2015
  • Place of Presentation: Wien, Austria
  • Year and Date: 2015-06-23
  • Int'l Joint Research
• [Presentation] シロイヌナズナ根毛におけるCa2+結合タンパク質PCaP2とホスファチジルイノシトール-4,5二リン酸の細胞内動態. (2015)
  • Author(s): 加藤真理子、柘植知彦、前島正義、青山卓史
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] ピロリン酸代謝関連酵素の機能欠失がピロリン酸濃度と生育に与える影響. (2015)
  • Author(s): 福田茉由、瀬上紹嗣、郡司玄、Ali Ferjani、前島正義
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] The H+-pyrophosphatase is necessary for normal growth of Arabidopsis thaliana. (2015)
  • Author(s): 浅岡真理子、瀬上紹嗣、Ali Ferjani, 前島正義
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] PPi opposes gluconeogenesis progression during oilseed mobilization in Arabidopsis. (2015)
  • Author(s): Ferjani, A., Kawade, K.. Oikawa, A., Asaoka, Takahashi, K., Ishida, M., Maeshima, M., Yokota-Hirai, M., Saito, K. and Tsukaya, H.
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] 根毛伸長時におけるシロイヌナズナCa2+結合タンパク質PCaP2とホスファチジルイノシトール-4,5二リン酸の細胞内動態. (2014)
  • Author(s): 加藤真理子、前島正義、青山卓史
  • Organizer: 植物脂質科学研究会シンポジウム
  • Place of Presentation: 静岡市産学交流センター
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] GFP二量体化による人為的な膜の接着と単量体化によって見えた真の液胞構造. (2014)
  • Author(s): 瀬上紹嗣、牧野沙知、三宅愛、浅岡真理子、前島正義
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
  • Invited
• [Presentation] 新規シグナル変換分子PCaP1は植物の水分屈性に関与している (2014)
  • Author(s): 田中奈月、高橋秀幸、前島正義
  • Organizer: 日本生化学会大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2014-10-15 – 2014-10-18
• [Presentation] リン酸欠乏時に発現上昇する根毛タンパク質RHPPの機能解析. (2014)
  • Author(s): 田中奈月、奥田祥平、Ferjani Ali、郡司玄、前島正義
  • Organizer: 植物学会
  • Place of Presentation: 明治大学
  • Year and Date: 2014-09-12 – 2014-09-14
• [Presentation] Characteristics of a root hair-less line of Arabidopsis thaliana under physiological stresses. (2014)
  • Author(s): Tanaka, N., Kato, M., Tomioka, R., Kurata, R., Fukao, Y., Aoyama, T., and Maeshima, M.
  • Organizer: Plant Biology Europe FESPB/EPSO 2014 Congress
  • Place of Presentation: Doublin, Irland
  • Year and Date: 2014-06-21 – 2014-06-27
• [Presentation] H+-pyrophosphatase is necessary for normal growth of Arabidopsis thaliana. (2014)
  • Author(s): Asaoka, M., Segami, S., Sato, C., Ferjani, A. and Maeshima, M.
  • Organizer: Plant Biology Europe FESPB/EPSO 2014 Congress
  • Place of Presentation: Doublin, Irland
  • Year and Date: 2014-06-21 – 2014-06-27
• [Book] Signaling and Communication in Plants, Vol. 22 (2014)
  • Author(s): Song, W.Y., Park J., Eisenach, C., Maeshima, M., Lee, Y., and Martinoia, E.
  • Total Pages: 331
  • Publisher: Springer-Verlag
• [Remarks] 細胞ダイナミクス研究室
  • URL: http://celld.agr.nagoya-u.ac.jp/index.html