| Budget Amount *help |
¥40,300,000 (Direct Cost: ¥31,000,000、Indirect Cost: ¥9,300,000)
Fiscal Year 2016: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2015: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2014: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
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| Outline of Final Research Achievements |
Plants regulate concentrations of metal ions in order to maintain their activities. We focused on the ion transport systems and ion-sensing machineries and revealed the followings. 1) The cytosolic histidine-rich loop changes its configuration and functions acts as a sensor of cytosolic zinc to maintain an essential level in the cytosol. This was evidence by in vivo examination using loss-of-function mutant plants. 2) New-type zinc transporter MTP12 forms a hetero-oligomer with MTP5 and functions as zinc transporter in the membrane of Golgi apparatus. 3) A newly identified calcium-binding protein PCaP1, which is stably associated with the plasma membrane, changes its conformation by binding with metal ions. The expression of PCaP1 gene is enhanced by increased copper levels and its loss-of-function mutant plants become sensitive to high copper levels. Also, PCaP1 is involved in the closing mechanism of stomata in leaves.
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