• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Search for the Phytophthora mating hormone receptors and signal transduction analysis

Research Project

Project/Area Number 26252015
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Bioorganic chemistry
Research InstitutionNagoya University

Principal Investigator

Ojika Makoto  名古屋大学, 生命農学研究科, 教授 (50152492)

Co-Investigator(Kenkyū-buntansha) 矢島 新  東京農業大学, 生命科学部, 准教授 (30328546)
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥32,890,000 (Direct Cost: ¥25,300,000、Indirect Cost: ¥7,590,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Fiscal Year 2014: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Keywords疫病菌 / Phytophthora / 交配ホルモン / 受容体 / 生合成 / 微生物 / 有性生殖 / トランスクリプトーム
Outline of Final Research Achievements

The plant pathogens of the genus Phytophthora, which are known to infect important crops and cause enormous damage, use mating hormones to perform sexual reproduction. With the aim of establishing the molecular basis of this biological phenomenon, we aimed to identify hormone receptors and biosynthetic enzymes. Biochemical approaches using fluorescent probes, etc. have not been successful due to the instability or low expression of proteins. Therefore, searching for receptors and biosynthetic genes by transcriptome analysis using RNA-Seq have performed, resulting in the finding several candidate genes. Experiments such as gene knockdown are in progress to identify the receptors and biosynthetic enzymes.

Academic Significance and Societal Importance of the Research Achievements

疫病菌(Phytophthora属糸状菌)は、様々な作物に感染して甚大な被害を与えることで知られる。特にジャガイモ疫病菌はアイルランドジャガイモ飢饉を引き起こした歴史をもつ。この植物病原菌は有性生殖を行うことで耐久性(グローバルな拡散性)と遺伝的多様性を獲得した有性胞子を作るので、疫病菌有性生殖の分子基盤の確立は、微生物有性生殖メカニズムの解明という学術的な意義に加え、疫病菌繁殖制御に繋がる重要課題である。

Report

(5 results)
  • 2018 Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (24 results)

All 2019 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (9 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 9 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (13 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Int'l Joint Research] Inner Mongolia Agricultural University(中国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Chinese Academy of Agricultural Sciences(中国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Specific Inhibitors of Sporangium Formation of Phytophthora capsici from Kalimeris indica2018

    • Author(s)
      Zhang H.、Farooq U.、Cheng L. H.、Ye Y.、Wang Y. C.、Kawagishi H.、Ojika M.、Qi J. H.
    • Journal Title

      Chemistry of Natural Compounds

      Volume: 54 Issue: 3 Pages: 567-569

    • DOI

      10.1007/s10600-018-2409-9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Universality of the Phytophthora mating hormones and diversity of their production profile2017

    • Author(s)
      Tomura Tomohiko、Molli Shylaja D.、Murata Ryo、Ojika Makoto
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 1-12

    • DOI

      10.1038/s41598-017-05380-3

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Phytophthora mating hormone α2 is an antagonist of the counterhormone α12016

    • Author(s)
      Zhang, Li; Yajima, Arata; Ojika, Makoto
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 80 Issue: 6 Pages: 1062-1065

    • DOI

      10.1080/09168451.2016.1146071

    • NAID

      120005868226

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The Full-size ABCG Transporters Nb-ABCG1 and Nb-ABCG2 function in Pre- and Post-invasion Defense against Phytophthora infestans in Nicotiana benthamiana2016

    • Author(s)
      Shibata Yusuke; Ojika Makoto; Sugiyama Akifumi; Yazaki Kazufumi; Jones David A; Kawakita Kazuhito; Takemoto Daigo
    • Journal Title

      Plant Cell

      Volume: 28 Issue: 5 Pages: 1163-1181

    • DOI

      10.1105/tpc.15.00721

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Heterologous production of the marine myxobacterial antibiotic haliangicin and its unnatural analogues generated by engineering of the biochemical pathway2016

    • Author(s)
      Sun, Y.; Feng, Z.; Tomura, T.; Suzuki, A.; Miyano, S.; Tsuge, T.; Mori, H.; Suh, J.-W.; Iizuka, T.; Fudou, R.; Ojika, M.
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 22091-22091

    • DOI

      10.1038/srep22091

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation and biosynthetic analysis of haliamide, a new PKS-NRPS hybrid metabolite from the marine myxobacterium Haliangium ochraceum2016

    • Author(s)
      Sun, Y.; Tomura, T.; Sato, J.; Iizuka, T.; Fudou, R. Ojika, M.
    • Journal Title

      Molecules

      Volume: 21 Issue: 1 Pages: 59-59

    • DOI

      10.3390/molecules21010059

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of the straightforward electro-transformation system for Phytophthora infestans and its comparison with the improved PEG/CaCl2 transformation2015

    • Author(s)
      Dong, Lei; Zhu, Xiangyuan; Cui, Haichen; Ojika, Makoto; Wang, Ruigang; Liu, Huirong
    • Journal Title

      J. Microbiol. Meth.

      Volume: 112 Pages: 83-86

    • DOI

      10.1016/j.mimet.2015.03.013

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methanol extract of mycelia from Phytophthora infestans-induced resistance in potato2015

    • Author(s)
      Monjil Mohammad Shahjahan; Nozawa Takeshi; Shibata Yusuke; Takemoto Daigo; Ojika Makoto; Kawakita Kazuhito
    • Journal Title

      Compt. ren. biol.

      Volume: 338 Pages: 185-196

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Recent progress in the chemistry and chemical biology of microbial signaling molecules: quorum sensing pheromones and microbial hormones2014

    • Author(s)
      Yajima, Arata
    • Journal Title

      Tetrahedron Lett.

      Volume: 55 Issue: 17 Pages: 2773-2780

    • DOI

      10.1016/j.tetlet.2014.03.051

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 病菌交配ホルモン生合成酵素の探索2019

    • Author(s)
      有吉 俊、戸村友彦、小鹿 一
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 疫病菌と宿主植物をつなぐ活性分子2018

    • Author(s)
      小鹿 一
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Two types of lipid elicitors with different functions from the potato pathogen Phytophthora infestans2018

    • Author(s)
      Makoto Ojika, Ryo Murata, Shiho Tenhiro, Mohammad S. Monjil, Takeshi Nozawa, Yasuhiro Ito, Shinya Wada, Yusuke Shibata, Daigo Takemoto, and Kazuhito Kawakita
    • Organizer
      30th International Symposium on the Chemistry of Natural Products and the 10th International Congress on Biodiversity
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 疫病菌交配ホルモンの生産プロファイルの多様性2017

    • Author(s)
      戸村友彦、大西裕貴、小鹿 一
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都
    • Related Report
      2016 Annual Research Report
  • [Presentation] 植物疫病菌交配ホルモンα1の生合成酵素の探索2016

    • Author(s)
      戸村友彦、小鹿 一
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
  • [Presentation] 疫病菌交配ホルモンα2は対ホルモンα1のアンタゴニストである2016

    • Author(s)
      張 莉、矢島 新、小鹿 一
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌
    • Year and Date
      2016-03-29
    • Related Report
      2015 Annual Research Report
  • [Presentation] 疫病菌交配ホルモンの産生を左右する因子2016

    • Author(s)
      戸村友彦、矢島 新、小鹿 一
    • Organizer
      第58回天然有機化合物討論会
    • Place of Presentation
      仙台
    • Related Report
      2016 Annual Research Report
  • [Presentation] 植物疫病菌の交配ホルモン感受性の多様性2016

    • Author(s)
      山田麻衣子、戸村友彦、大西裕貴、小鹿 一
    • Organizer
      日本農芸化学会中部支部第177回例会
    • Place of Presentation
      名古屋
    • Related Report
      2016 Annual Research Report
  • [Presentation] Regulatory factors that control asexual reproduction of the plant pathogen Phytophthora2015

    • Author(s)
      Makoto Ojika, Rika Iwai, Chunguang Han
    • Organizer
      PACIFICHEM 2015
    • Place of Presentation
      ハワイ、ホノルル
    • Year and Date
      2015-12-19
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel cytotoxic diterpene from a marine myxobacterium, Enhygromyxa sp.2015

    • Author(s)
      Tomohiko Tomura, Ryosuke Fudou, Takashi Iizuka, Makoto Ojika
    • Organizer
      PACIFICHEM 2015
    • Place of Presentation
      ハワイ、ホノルル
    • Year and Date
      2015-12-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物疫病菌交配ホルモンの受容体および生合成酵素の探索2015

    • Author(s)
      戸村友彦、張 莉、山田麻衣子、矢島 新、小鹿 一
    • Organizer
      第57回天然有機化合物討論会
    • Place of Presentation
      横浜
    • Year and Date
      2015-09-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 野菜ジュースからの植物疫病菌有性生殖促進物質の探索2015

    • Author(s)
      天廣 志保、岩井 里佳、小鹿 一
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山市
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 疫病菌の交配ホルモン2014

    • Author(s)
      小鹿 一
    • Organizer
      第49回天然物化学談話会
    • Place of Presentation
      倉敷市
    • Year and Date
      2014-07-02 – 2014-07-04
    • Related Report
      2014 Annual Research Report
    • Invited

URL: 

Published: 2014-04-04   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi