| Budget Amount *help |
¥39,780,000 (Direct Cost: ¥30,600,000、Indirect Cost: ¥9,180,000)
Fiscal Year 2017: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2016: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2015: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2014: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Outline of Final Research Achievements |
Prostaglandin D2 (PGD2) mediates important effector and regulatory functions during inflammatory response. In the present study, we studied non-enzymatic conversion of PGD2 and identified Δ12-PGD2, a structural isomer of PGD2, as one of the major albumin-dependent metabolites. The formation of Δ12-PGD2 was demonstrated in the activated RBL-2H3 mast cells. Based on the finding that the medium conditioned by activated RBL-2H3 mast cells enhanced the nerve growth factor-induced neuritogenesis of PC12 cells, we attempted to isolate an active compound from the mast cell conditioned culture medium and identified 15-deoxy-Δ12,14-PGJ2 (15d-PGJ2) as a potential enhancer of neuritogenesis. We have established a mechanism in which 15d-PGJ2-induced neuritogenesis is regulated by two sets of mechanisms, one for the translocation of a calcium channel (TRPV1) into the cell surface by NGF and one for the activation of TRPV1 by 15d-PGJ2.
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