Basic studies on anti-inflammatory lipid mediators
Project/Area Number |
26252018
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Food science
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Research Institution | The University of Tokyo (2015-2017) Nagoya University (2014) |
Principal Investigator |
Uchida Koji 東京大学, 大学院農学生命科学研究科(農学部), 教授 (40203533)
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Research Collaborator |
SHIBATA Takahiro 名古屋大学, 大学院生命農学研究科, 准教授 (80447838)
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Project Period (FY) |
2014-06-27 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥39,780,000 (Direct Cost: ¥30,600,000、Indirect Cost: ¥9,180,000)
Fiscal Year 2017: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2016: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2015: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2014: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
|
Keywords | 脂質メディエーター / プロスタグランジン / 抗炎症 / タンパク質修飾 / 神経細胞分化 |
Outline of Final Research Achievements |
Prostaglandin D2 (PGD2) mediates important effector and regulatory functions during inflammatory response. In the present study, we studied non-enzymatic conversion of PGD2 and identified Δ12-PGD2, a structural isomer of PGD2, as one of the major albumin-dependent metabolites. The formation of Δ12-PGD2 was demonstrated in the activated RBL-2H3 mast cells. Based on the finding that the medium conditioned by activated RBL-2H3 mast cells enhanced the nerve growth factor-induced neuritogenesis of PC12 cells, we attempted to isolate an active compound from the mast cell conditioned culture medium and identified 15-deoxy-Δ12,14-PGJ2 (15d-PGJ2) as a potential enhancer of neuritogenesis. We have established a mechanism in which 15d-PGJ2-induced neuritogenesis is regulated by two sets of mechanisms, one for the translocation of a calcium channel (TRPV1) into the cell surface by NGF and one for the activation of TRPV1 by 15d-PGJ2.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Abnormal myofiber morphology and limb dysfunction in claudication.2015
Author(s)
Koutakis, P., Myers, S. A., Cluff, K., Ha, D. M., Haynatzki, G., McComb, R. D., Uchida, K., Miserlis, D., Papoutsi, E., Johanning, J. M., Casale, G. P., and Pipinos, I. I.
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Journal Title
J. Surg. Res.
Volume: J. Surg. Res.
Issue: 1
Pages: 172-179
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Generation of adducts of 4-hydroxy-2-nonenal with heat shock 60kDa protein 1 in human promyelocytic HL-60 and monocytic THP-1 cell lines.2015
Author(s)
Arcaro, A., Daga, M., Paolo Cetrangolo, G., Ciamporcero, E., Lepore, A., Pizzimenti, A., Petrella, C., Graf, M., Uchida, U., Mamone, G., Ferranti, P., Julian Ames, R., Palumbo, G., BarreraG., and Gentile, F.
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Journal Title
Oxid Med. Cell. Longev.
Volume: 2015
Pages: 296146-296146
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Direct exposure of non-equilibrium atmospheric pressure plasma confers simultaneous oxidative and ultraviolet modifications in biomolecules2014
Author(s)
Yasumasa Okazaki, Yue Wang, Hiromasa Tanaka, Masaaki Mizuno, Kae Nakamura, Hiroaki Kajiyama, Hiroyuki Kano, Koji Uchida, Fumitaka Kikkawa, Masaru Hori, Shinya Toyokuni
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Journal Title
Journal of Clinical Biochemistry and Nutrition
Volume: 55
Issue: 3
Pages: 207-215
DOI
NAID
ISSN
0912-0009, 1880-5086
Related Report
Peer Reviewed / Open Access
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[Journal Article] Toll-like receptors as a target of food-derived anti-Inflammatory compounds.2014
Author(s)
Shibata, T., Nakashima, F., Honda, K., Lu, Y. J., Kondo, T., Ushida, Y., Aizawa, K., Suganuma, H., Oe, S., Tanaka, H., Takahashi, T. and Uchida, K.
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Journal Title
J. Biol. Chem.
Volume: 289
Issue: 47
Pages: 32757-32772
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ferritin-stimulated lipid peroxidation, lysosomal leak, and macroautophagy promote lysosomal "metastability" in primary hepatocytes determining in vitro cell survival.2014
Author(s)
Krenn, M. A., Schürz, M., Teufl, B., Uchida, K., Eckl, P. M., Bresgen, N.
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Journal Title
Free Radic. Biol. Med.
Volume: 80
Pages: 48-58
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Functional interaction between cyclooxygenase-2 and p53 in response to an endogenous electrophile2014
Author(s)
Kumagai, T., Usami, H., Matsukawa, N., Nakashima, F., Chikazawa, M., Shibata, T., Noguchi, N., and Uchida, K.
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Journal Title
Redox Biol.
Volume: 4
Pages: 74-86
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Elastin modification by 4-hydroxynonenal in hairless mice exposed to UV-A. Role in photoaging and actinic elastosis.2014
Author(s)
Larroque-Cardoso, P., Camaré, C., Nadal-WollboldF., Grazide, M. H., Pucelle, M., Garoby-Salom, S., Bogdanowicz, P., Josse, G., Schmitt, A. M., Uchida, K., Zarkovic, K., Salvayre, R., and Nègre-Salvayre, A.
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Journal Title
J. Invest. Dermatol.
Volume: 135
Issue: 7
Pages: 1873-1881
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Book] 細胞工学2014
Author(s)
熊谷嘉人、内田浩二
Total Pages
440
Publisher
学研メディカル秀潤社
Related Report
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