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The use of stable isotope ratios of nitrate in the forest ecosystems for the better understanding of nitrogen status

Research Project

Project/Area Number 26252020
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Forest science
Research InstitutionKyoto University (2015-2016)
Tokyo University of Agriculture and Technology (2014)

Principal Investigator

Koba Keisuke  京都大学, 生態学研究センター, 教授 (90311745)

Co-Investigator(Kenkyū-buntansha) 戸田 浩人  東京農工大学, (連合)農学研究科(研究院), 教授 (00237091)
大手 信人  京都大学, 情報学研究科, 教授 (10233199)
五味 高志  東京農工大学, (連合)農学研究科(研究院), 准教授 (30378921)
勝山 正則  京都大学, 学際融合教育研究推進センター, 准教授 (40425426)
徳地 直子  京都大学, フィールド科学教育研究センター, 教授 (60237071)
柴田 英昭  北海道大学, 北方生物圏フィールド科学センター, 教授 (70281798)
楊 宗興  東京農工大学, (連合)農学研究科(研究院), 教授 (50260526)
Co-Investigator(Renkei-kenkyūsha) INAGAKI Yoshiyuki  森林総合研究所, 立地環境研究領域, 主任研究員 (00353590)
TATENO Ryunosuke  京都大学, フィールド科学教育研究センター, 准教授 (60390712)
WATANABE Mirai  国立環境研究所, 地域環境研究センター, 主任研究員 (50455250)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥39,910,000 (Direct Cost: ¥30,700,000、Indirect Cost: ¥9,210,000)
Fiscal Year 2016: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2015: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2014: ¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Keywords窒素循環 / 脱窒 / 硝化 / 集水域 / 安定同位体 / 窒素飽和 / 物質循環・フラックス / 窒素沈着
Outline of Final Research Achievements

It is crucial to explore the nitrogen status of the forest ecosystems due to the management of the healthy forested ecosystems. However, due to the high spatiotemporal variations in nitrogen dynamics even in a small watershed, it is difficult to grasp its nitrogen status. We applied the isotope technique to estimate the gross nitrification rate and the denitrification with the help of the isotopic fractionation factors for different nitrogen processes occurring in the watershed. We found the high gross nitrification rates and denitrification rates in the watershed with more N deposition. However, the efficiency of the nitrogen removal by the denitrification was relatively low in high N deposition sites, indicating that the removal of nitrogen cannot work effectively in nitrogen-polluted sites.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (29 results)

All 2017 2016 2015 2014 Other

All Journal Article (14 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 14 results,  Acknowledgement Compliant: 6 results,  Open Access: 3 results) Presentation (10 results) (of which Invited: 1 results) Remarks (5 results)

  • [Journal Article] Hybrid Nitrous Oxide Production from a Partial Nitrifying Bioreactor: Hydroxylamine Interactions with Nitrite2017

    • Author(s)
      A. Terada, S. Sugawara, K. Hojo, Y. Takeuchi, S. Riya, W. F. HarperJr., T. Yamamoto, M. Kuroiwa, Isobe, C. Katsuyama, Y. Suwa, K. Koba and M. Hosomi
    • Journal Title

      Environmental Science and Technology

      Volume: 51 Issue: 5 Pages: 2748-2756

    • DOI

      10.1021/acs.est.6b05521

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Nitrogen and oxygen isotope effects of ammonia oxidation by thermophilic Thaumarchaeota from a geothermal water stream2016

    • Author(s)
      Nishizawa M, Sakai S, Konno U, Nakahara N, Takaki Y, Saito Y, Imachi H, Tasumi E, Makabe A, Koba K and Takai K
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 82 Issue: 15 Pages: 4492-4504

    • DOI

      10.1128/aem.00250-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Stable isotope and isotopomer distributions reveal differential N2O dynamics in two oxygen-deficient lake basins2016

    • Author(s)
      C. B. Wenk, C. H. Frame, K. Koba, K. L. Casciotti, M. Veronesi, H. Niemann, C. J. Schubert, N. Yoshida, S. Toyoda, A. Makabe, J. Zopfi, and M. F. Lehmann
    • Journal Title

      Limnology and Oceanography

      Volume: 61 Issue: 5 Pages: 1735-1749

    • DOI

      10.1002/lno.10329

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Relationships among pH, minerals, and carbon in soils from tundra to boreal forest2016

    • Author(s)
      S. Hobara, K. Kushida, Y. Kim, K. Koba, B.-Y. Lee and N. Ae
    • Journal Title

      Ecosystems

      Volume: 印刷中

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Variation in leaf and soil δ15N in diverse tree species in a lowland dipterocarp rainforest, Malaysia.2016

    • Author(s)
      A. Tanaka-Oda , T. Kenzo, Y. Inoue, M. Yano, K. Koba, and T. Ichie.
    • Journal Title

      Trees

      Volume: 30(2) Issue: 2 Pages: 509-522

    • DOI

      10.1007/s00468-015-1298-9

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ecological interpretations of nitrogen isotope ratios of terrestrial plants and soils2016

    • Author(s)
      J. M. Craine, E. N. J. Brookshire, M. D. Cramer, N. J. Hasselquist, K. Koba, E. Marin-Spiotta, and L. Wang
    • Journal Title

      Plant and Soil

      Volume: 396(1) Issue: 1-2 Pages: 1-26

    • DOI

      10.1007/s11104-015-2542-1

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Isotopocule analysis of biologically produced nitrous oxide in various environments2015

    • Author(s)
      Sakae Toyoda, Naohiro Yoshida, and Keisuke Koba
    • Journal Title

      Mass Spectrometry Reviews

      Volume: - Issue: 2 Pages: 135-160

    • DOI

      10.1002/mas.21459

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Drivers of Atmospheric Nitrate Processing and Export in Forested Catchments2015

    • Author(s)
      L. A. Rose, S. D. Sebestyen, E. M. Elliott, K. Koba.
    • Journal Title

      Water Resources Research

      Volume: 51 Issue: 2 Pages: 1333-1352

    • DOI

      10.1002/2014wr015716

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Convergence of soil nitrogen isotopes across global climate gradients2015

    • Author(s)
      J. M. Craine, A. J. Elmore, L. Wang, L. Augusto, W. T. Baisden, E. N. J. Brookshire, M. D. Cramer, N. J. Hasselquist, E. A. Hobbie, A. Kahmen, K. Koba, et al.
    • Journal Title

      Scientific Reports

      Volume: 5 Issue: 1 Pages: 8280-8280

    • DOI

      10.1038/srep08280

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Higher diversity and abundance of denitrifying microorganisms in environments than considered previously2015

    • Author(s)
      Wei, W., K. Isobe, T. Nishizawa, L. Zhu, Y. Shiratori, N. Ohte, K. Koba, S. Otsuka, and K. Senoo
    • Journal Title

      The ISME Journal

      Volume: 10 March 2015 Issue: 9 Pages: 1-12

    • DOI

      10.1038/ismej.2015.9

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Hadal biosphere: insight into the microbial ecosystem in the deepest ocean on Earth.2015

    • Author(s)
      Nunoura T, Takaki Y, Hirai M, Shigeru S, Makabe A, Koide O, Kikuchi T, Miyazaki J, Koba K, Yoshida N, Sunamura M, Takai K
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 112 Issue: 11

    • DOI

      10.1073/pnas.1421816112

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Microbial denitrification dominates nitrate losses from forest ecosystems2015

    • Author(s)
      Fang Y, Koba K, Makabe A, Takahashi C, Zhu W, Hayashi T, Hokari AA, Urakawa R, Bai E, Houlton BZ, Xi D, Zhang S, Matsushita K, Tu Y, Liu D, Zhu F, Wang Z, Zhou G, Chen D, Makita T, Toda H, Liu X, Chena Q, Zhang D, Li Y, Yoh M
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 112 Issue: 5 Pages: 1470-1474

    • DOI

      10.1073/pnas.1416776112

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel pyrroloquinoline quinone-dependent 2-keto-D-glucose dehydrogenase from Pseudomonas aureofaciens2015

    • Author(s)
      Kiwamu Umezawa, Kouta Takeda, Takuya Ishida, Naoki Sunagawa, Akiko Makave, Kazuo Isobe, Keisuke Koba, Hiroyuki Ohno, Masahiro Samejima, Nobuhumi Nakamura, Kiyohiko Igarashi, Makoto Yoshida
    • Journal Title

      Journal of Bacteriology

      Volume: 197(8) Issue: 8 Pages: 1322-1329

    • DOI

      10.1128/jb.02376-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isotopomeric characterization of nitrous oxide produced by reaction of enzymes extracted from nitrifying and denitrifying bacteria2014

    • Author(s)
      T. Yamazaki, T. Hozuki, K. Arai, S. Toyoda, K. Koba, T. Fujiwara, N. Yoshida
    • Journal Title

      Biogeosciences

      Volume: 11 Issue: 10 Pages: 2679-2689

    • DOI

      10.5194/bg-11-2679-2014

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 微量溶存窒素化合物の同位体比測定とその応用2016

    • Author(s)
      木庭啓介
    • Organizer
      第6回同位体環境学シンポジウム
    • Place of Presentation
      総合地球環境学研究所(京都市北区)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 土壌微生物バイオマスの窒素安定同位体比:ストイキオメトリーと同位体比の接点2015

    • Author(s)
      木庭啓介
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      土浦市亀城プラザ(茨城県・土浦市)
    • Year and Date
      2015-10-20
    • Related Report
      2015 Annual Research Report
  • [Presentation] 土壌DNAにおける窒素安定同位体比測定法の開発と森林土壌への応用2015

    • Author(s)
      村田 基樹
    • Organizer
      日本微生物生態学会
    • Place of Presentation
      土浦市亀城プラザ(茨城県・土浦市)
    • Year and Date
      2015-10-19
    • Related Report
      2015 Annual Research Report
  • [Presentation] Effects of environmental factors on production of dissolved N2-a product of denitrification. A case study in Tama River2015

    • Author(s)
      CONG, Thuan NGUYEN、Keisuke KOBA and others
    • Organizer
      日本地球惑星合同大会
    • Place of Presentation
      幕張メッセ(千葉県・幕張市)
    • Year and Date
      2015-05-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 窒素安定同位体比を用いた植物の窒素利用様式の把握2015

    • Author(s)
      木庭 亜弥美、木庭 啓介ほか
    • Organizer
      日本地球惑星合同大会
    • Place of Presentation
      幕張メッセ(千葉県・幕張市)
    • Year and Date
      2015-05-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] NOxの窒素安定同位体比測定技術の開発2015

    • Author(s)
      村田 恵理子、木庭 啓介ほか
    • Organizer
      日本地球惑星合同大会
    • Place of Presentation
      幕張メッセ(千葉県・幕張市)
    • Year and Date
      2015-05-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] NOxの窒素安定同位体比測定技術の開発2015

    • Author(s)
      村田 恵理子,木庭啓介,ほか
    • Organizer
      地球惑星科学連合合同大会
    • Place of Presentation
      幕張メッセ
    • Year and Date
      2015-05-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] 窒素安定同位体比を用いた植物の窒素利用様式の把握2015

    • Author(s)
      木庭 亜弥美,木庭啓介,ほか
    • Organizer
      地球惑星科学連合合同大会
    • Place of Presentation
      幕張メッセ
    • Year and Date
      2015-05-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Aspergillus oryzae の窒素安定同位体比における窒素可給性の影響2015

    • Author(s)
      篠田 一輝、木庭 啓介ほか
    • Organizer
      日本地球惑星合同大会
    • Place of Presentation
      幕張メッセ(千葉県・幕張市)
    • Year and Date
      2015-05-26
    • Related Report
      2015 Annual Research Report
  • [Presentation] 土壌微生物バイオマスの窒素安定同位体比:ストイキオメトリーと同位体比の接点2015

    • Author(s)
      木庭啓介
    • Organizer
      日本地球惑星合同大会
    • Place of Presentation
      幕張メッセ(千葉県・幕張市)
    • Year and Date
      2015-05-26
    • Related Report
      2015 Annual Research Report
  • [Remarks] 京都大学生態学研究センター木庭研究室

    • URL

      http://www.ecology.kyoto-u.ac.jp/~keikoba/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 木庭研究室Website(京大生態学研究センター)

    • URL

      http://www.ecology.kyoto-u.ac.jp/~keikoba/index.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 東京農工大学木庭研究室

    • URL

      http://ecosystems.lab.tuat.ac.jp/index.html

    • Related Report
      2014 Annual Research Report
  • [Remarks] 超深海・海溝生命圏を発見 ―マリアナ海溝の超深海水塊に独自の微生物生態系―

    • URL

      http://www.jamstec.go.jp/j/about/press_release/20150224/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 新たな窒素循環解析法の構築で森林の窒素除去能力を解明

    • URL

      http://www.tuat.ac.jp/disclosure/pressrelease/2014/20150107195447/index.html

    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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