Mass production of genetically sterile mutant fish by germ-cell transplantation
| Project/Area Number |
26252033
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Aquatic life science
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| Research Institution | Tokyo University of Marine Science and Technology |
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Principal Investigator |
Yoshizaki Goro 東京海洋大学, 学術研究院, 教授 (70281003)
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| Co-Investigator(Kenkyū-buntansha) |
北野 健 熊本大学, 自然科学研究科, 准教授 (40336219)
吉浦 康寿 国立研究開発法人水産研究・教育機構, その他部局等, 研究員 (90372052)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥40,430,000 (Direct Cost: ¥31,100,000、Indirect Cost: ¥9,330,000)
Fiscal Year 2016: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2015: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Fiscal Year 2014: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
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| Keywords | 不妊魚 / メダカ / トラフグ / ゲノム編集 / FSH受容体 / 人工ヌクレアーゼ / 代理親魚 / 生殖細胞移植 / GTH受容体 / サケ科魚類 |
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| Outline of Final Research Achievements |
The escape of captive fish is a serious global problem, as some of the farmed fish are highly domesticated. While the adoption of sterile fish seeds is desirable for avoiding genetic pollution, the mass production of sterile seeds is difficult. We employed medaka that were mutants for FSHR, produced by TILLING, as the female homozygous mutants are completely sterile. Since the homozygous males are fertile, production of sterile seeds can be achieved by mating heterozygous females with homozygous XX sex-reversed males. However, only half of the resulting F1 individuals are homozygous. To overcome this problem, we applied germ cell transplantation. We transplanted XX spermatogonia isolated from sex-reversed mutants homozygous for FSHR into sterile triploid female recipients. The resulting surrogate females produced only FSHR-mutant eggs.Thus, we confirmed that germ cell transplantation is a powerful technique for mass production of genetically sterile fish.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] Production of germ cell-deficient salmonids by dead end gene knockdown, and their use as recipients for germ cell transplantation2016
Author(s)
Yoshizaki, G., Takashiba, K., Shimamori, S., Fujinuma, K., Shikina, S., Okutsu, T., Kume, S., Hayashi, M.
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Journal Title
Molecular Reproduction and Development
Volume: 83
Issue: 4
Pages: 298-311
DOI
Related Report
Peer Reviewed
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[Journal Article] Germ Cell-Specific Excision of loxP-Flanked Transgenes in Rainbow Trout Oncorhynchus mykiss2016
Author(s)
Katayama, N., Kume, S., Hattori-Ihara, S., Sadaie, S., Hayashi, M., Yoshizaki, G.
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Journal Title
Biology of Reproduction
Volume: 94
Issue: 4
Pages: 1-12
DOI
Related Report
Peer Reviewed
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[Presentation] Targeted rnutagenesis using easy-to-use.2016
Author(s)
Digmayer M, Yoshizaki G, Qian L, Katayama N, Nobrega RH
Organizer
VI International Symposium on Animal Biology of Reproduction (ISABR 2016)
Place of Presentation
Campos do Jordao, SP, Brazil
Year and Date
2016-11-06
Related Report
Int'l Joint Research
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[Presentation] Germ cell manipulation in fish.2016
Author(s)
Goro Yoshizaki
Organizer
The 22th Japanese medaka and zebrafish meeting
Place of Presentation
Okazaki, Japan (National Institute for Basic Biology)
Year and Date
2016-08-20
Related Report
Int'l Joint Research / Invited
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