Studies on the development of aquafeed with consideraton of rearing condition and low load to environment

• Project/Area Number: 26252034
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Satoh Shuichi 東京海洋大学, 学術研究院, 教授 (80154053)
• Co-Investigator(Kenkyū-buntansha): 廣野 育生 東京海洋大学, 学術研究院, 教授 (00270926) ; 芳賀 穣 東京海洋大学, 学術研究院, 准教授 (00432063) ; 吉崎 悟朗 東京海洋大学, 学術研究院, 教授 (70281003)
• Project Period (FY): 2014-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥39,520,000 (Direct Cost: ¥30,400,000、Indirect Cost: ¥9,120,000); Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2017: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000); Fiscal Year 2014: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 養魚飼料 / 環境負荷低減 / 必須脂肪酸代謝 / アミノ酸代謝 / 無魚粉飼料 / 無魚粉無魚油飼料 / 無魚粉 / 無魚油 / 脂肪酸 / アミノ酸 / 消化吸収率 / 環境負荷低減飼料 / 環境適応 / 脂肪酸代謝 / 塩分

Outline of Final Research Achievements

The project was conducted to decrease the load from aquaculture site by developing the non-fish meal and non-fish oil feed for fish, and surveyed the optimal condition for rearing. As protein sources, soybean meal, corn gluten meal, and soy protein concentrate which contain low phosphorus are considered to suitable. As lipid source, schizochytrium meal is considered to be suitable because of high docosahexaenoic acid contents. It was succeeded to develop the non-fish meal and non-fish oil feed for red sea brea by formulate plant protein sources and schizochytrium meal.

Academic Significance and Societal Importance of the Research Achievements

淡水魚の栄養要求と海水魚の栄養要求は異なり、そのため海水魚の無魚粉無魚油飼料の作製は困難とされてきた。そこで、海水魚と淡水魚の栄養素の代謝経路の相違を詳細に検討した。そして、海水魚では最終代謝産物として合成されないタウリンやドコサヘキサエン酸を含んだ原料を配合することで無魚粉無魚油飼料の開発に成功した。これらのことは、魚類栄養学における最新の知見を提供するばかりではなく、魚に依存しない養殖用飼料の開発に貢献するものと考えられる。

Research Products

• [Journal Article] Effect of nucleotides supplementation to low fish meal feed on long-chain polyunsaturated fatty acids composition of juvenile rainbow trout Oncorhynchus mykiss (2019)
  • Author(s): A.Rudwanudin, Y.Haga, S.Satoh
  • Journal Title: Aquaculture Research Volume: 50 Issue: 8 Pages: 2218-2230
  • DOI: 10.1111/are.14103
  • Peer Reviewed
• [Journal Article] Cysteamine dioxygenase as enzymes for taurine 1 synthesis and the negative effect of high dietary cysteamine on growth and body shape of the common carp, Cyprinus carpio (2019)
  • Author(s): M.M. Gonzales-Plasus, H. Kondo, I. Hirono, S. Satoh, Y.Haga
  • Journal Title: Aquaculture Science Volume: 65
  • Peer Reviewed
• [Journal Article] Molecular characterization and tissue distribution of cysteamine dioxygenase (ADO) in common carp Cyprius carpio (2019)
  • Author(s): M.M. Gonzales-Plasus, Y.Haga, H. Kondo, I. Hirono, S. Satoh
  • Journal Title: The Palawan Scientist Volume: 11
  • Peer Reviewed
• [Journal Article] Effects of arginine supplementation on growth performance and plasma arginine, ornithine, and citrulline dynamics of rainbow trout (2019)
  • Author(s): I.A.Fauzi, Y.Haga, S.Satoh
  • Journal Title: Fisheries Science Volume: 50 Issue: 4 Pages: 1277-1290
  • DOI: 10.1111/are.14004
  • Peer Reviewed
• [Journal Article] Periodic changes in the growth performance and biochemical composition of juvenile red sea bream Pagrus major fed non-heatedand heated squid and krill meal-based diets (2018)
  • Author(s): J.H.Cho, Y.Haga, R.Masuda, and S.Satoh
  • Journal Title: Fisheries Science Volume: 84 Issue: 4 Pages: 699-713
  • DOI: 10.1007/s12562-018-1205-6
  • Peer Reviewed
• [Journal Article] 201.Interactive effects of salinity and complete fishmeal replacement on growth, food consumption, and gene expression of hepatic IGF-I, IGF-II and growth hormone receptors in Nile tilapia, Oreochromis niloticus (L.) (2018)
  • Author(s): S.S.Herath, Y.Haga, and S.Satoh
  • Journal Title: Aquaculture Research Volume: 49 Issue: 6 Pages: 2128-2139
  • DOI: 10.1111/are.13667
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 202.Periodic changes in the growth performance and biochemical composition of juvenile red sea bream Pagrus major fed non-heatedand heated squid and krill meal-based diets (2018)
  • Author(s): J.H.Cho, Y.Haga, R.Masuda, and S.Satoh
  • Journal Title: Fisheries Science Volume: 84
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 200.Cloning and functional characterization of fads2 desaturase 1 and elovl5 elongase fromJapanese flounder Paralichthys olivaceus (2017)
  • Author(s): N. Kabeya, M.Chiba, Y. Haga, S. Satoh., and G. Yoshizaki
  • Journal Title: Comparative Biochemistry and Physiology Part B Volume: 214B Pages: 36-47
  • DOI: 10.1016/j.cbpb.2017.09.002
  • Peer Reviewed
• [Journal Article] Evaluation of gastric evacuation and digestion of extruded pellet composed of a single protein source for juvenile red seabream Pagrus major (2017)
  • Author(s): K.Touhata, T.Koshiishi, S.Satoh, and N.Ishida
  • Journal Title: Aquaculture Science Volume: 65 Pages: 1-9
  • NAID: 130006516272
  • Peer Reviewed
• [Journal Article] Effect of supplementation with enzyme complex to non-fish meal diet in adult red sea bream Pagrus major (2017)
  • Author(s): K.Matsukura, S.Iino, Y.Haga, R.Kitajima, and S.Satoh
  • Journal Title: Aquaculture Science Volume: 65 Pages: 19-27
  • NAID: 130006516275
  • Peer Reviewed
• [Journal Article] Effect of long-term feeding of corn co-product-based diets on growth, fillet color, and fatty acid and amino acid composition of Nile tilapia, Oreochromis niloticus (2016)
  • Author(s): S.S.Herath, Y.Haga, and S.Sato
  • Journal Title: Aquaculture Volume: 464 Pages: 205-212
  • DOI: 10.1016/j.aquaculture.2016.06.032
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Potential use of corn co-products for fish meal-free diets for juvenile Nile tilapia, Oreochromis niloticus (2016)
  • Author(s): S.S.Herath, Y.Haga, and S.Satoh
  • Journal Title: Fisheries Science Volume: 82 Issue: 5 Pages: 811-818
  • DOI: 10.1007/s12562-016-1008-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 178.Preliminary nutritional evaluation of rubber seed and defatted rubber seed meals as plant protein sources for common carp Cyprinus carpio L. juvenile diet (2015)
  • Author(s): M. A. Suprayudi, C.Inara, J.Ekasari, N.Priyoutomo, Y.Haga, T.Takeuchi, and S.Satoh
  • Journal Title: Aquaculture Research Volume: 46 Issue: 12 Pages: 2971-2981
  • DOI: 10.1111/are.12452
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Replacement of fish meal with rendered animal protein and plant protein sources on growth response, biological indices and amino acid availability of rainbow trout Oncorhynchus mykiss (2015)
  • Author(s): F.Lu, Y.Haga, S.Satoh
  • Journal Title: Fisheries Science Volume: 81 Issue: 1 Pages: 95-105
  • DOI: 10.1007/s12562-014-0818-7
  • Peer Reviewed
• [Journal Article] 180.Transgenic modification of the n-3 HUFA biosynthetic pathway in nibe croaker larvae: Improved DPA (docosapentaenoic acid; 22:5n-3) production (2015)
  • Author(s): N.Kabeya, Y.Takeuchi, R.Yazawa, Y.Haga, S.Satoh, and G.Yoshizaki
  • Journal Title: Aquaculture Nutrition Volume: 22 Issue: 2 Pages: 482-478
  • DOI: 10.1111/anu.12273
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 184.Polyunsaturated fatty acid metabolism in a marine teleost, Nibe croaker Nibea mitsukurii: Functional characterization of Fads2 desaturase and Elovl5 and Elovl4 elongases (2015)
  • Author(s): N. Kabeya, Y. Yamamoto, S.F. Cummins, A. Elizur, R. Yazawa, Y. Takeuchi, Y. Haga, Shuichi Satoh and G. Yoshizaki
  • Journal Title: Comparative Biochemistry Part B Volume: 188 Pages: 37-45
  • DOI: 10.1016/j.cbpb.2015.06.005
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 185.Detection of transgenic and endogenous plant DNA in blood and organs of Nile tilapia, Oreochromis niloticus fed a diet formulated with genetically modified soybean meal (2015)
  • Author(s): I.Suharman, S.Satoh, Y.Haga, I.Hirono, Z.A.Muchlisin
  • Journal Title: AACL Bioflux Volume: 8 Pages: 160-168
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] 完全無魚粉化extruded-pellet（EP）飼料を用いたブリSeriola 28 quinqueradiataの長期飼育試験 (2015)
  • Author(s): 津﨑龍雄・吉田一範・堀田卓朗・中川雅弘・山田真之・佐藤秀一・輿石友彦・前野幸二・秋山敏男・石田典子
  • Journal Title: 水産増殖 Volume: 63 Pages: 29-38
  • Peer Reviewed
• [Journal Article] Isolation, molecular characterization of cysteine sulfinic acid decarboxylase (CSD) of red sea bream Pagrus major and yellowtail Seriola quinqueradiata and expression analysis of CSD from sereal marine fish species (2015)
  • Author(s): Y.Haga, H.Kondo, A.Kumagai, N.Satoh, I.Hirono, and S.Satoh
  • Journal Title: Aquaculture Volume: 印刷中 Pages: 8-17
  • DOI: 10.1016/j.aquaculture.2015.04.004
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 魚粉高騰危機を回避するブリ用の魚粉削減飼料の開発 (2015)
  • Author(s): 佐藤秀一
  • Journal Title: 養殖ビジネス Volume: 52(3) Pages: 6-9
• [Presentation] ブリ用無魚粉飼料における魚油代替に関する研究 (2019)
  • Author(s): 松吉純平、輿石友彦、宮原治郎、R.Kitagima、佐藤秀一、芳賀穣
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] マダイ稚魚の成長成績およびタウリン含量に対するタウリン前駆物質の給餌効果 (2019)
  • Author(s): 伊藤智子、芳賀穣、益田玲爾、佐藤秀一
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] Effect of pyrimidine mucliotides supplementation in high or low fish oil based-diets on growth and fatty acid composition of juvenile raibow trout Oncorhychus mykiss (2019)
  • Author(s): A.Rudwanudin, H.Kasuya,Y.Haga, S.Satoh
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] Effect of different thermal condition during extrusion processing on soybean and corngluten meal on their nutritional value for juvenile rainbow trout (Oncorhychus mykiss) (2019)
  • Author(s): T.R.Santizo, S.Satoh, Y.Haga
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] Non-fish meal and non-fish oil deit development for red sea bream, Pagrus major, with microalgae species (2019)
  • Author(s): T.Seong, K.Waki, T.Hashimoto, Y.Haga, S.Satoh
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] Effect of intraperitoneal injection of sulfur compoiunds on taurine synthesis in juvenile red sea bream (Pagrus major) (2018)
  • Author(s): T.Itoh, H.Yaga, R.Masuda, S.Satoh
  • Organizer: International Symposium on Fish Nutrition and Feeding 2018
  • Int'l Joint Research
• [Presentation] Effect of dietary fish oil replacement by microalgae, schizochytrium sp. on growth performance, body composition and fatty acid profile of juvenile red seabream, Pagrus major (2018)
  • Author(s): T.Seong, R.Kitagima, Y.Haga, S.Satoh
  • Organizer: International Symposium on Fish Nutrition and Feeding 2018
  • Int'l Joint Research
• [Presentation] Effect of taurine supplementation to non or low fishmeal diet on growth, intestinalmorphology and cytokines gene expression of juvenile res sea bream, Pagrus major (2018)
  • Author(s): F.Li, H.Kond, Y.Haga, S.Satoh
  • Organizer: International Symposium on Fish Nutrition and Feeding 2018
  • Int'l Joint Research
• [Presentation] Effect of dietary nucleotide on growth and fatty acid composition of juvenile raibow trout Oncorhnchus mykiss (2018)
  • Author(s): A.Rudwanudin, Y.Haga, S.Satoh
  • Organizer: International Symposium on Fish Nutrition and Feeding 2018
• [Presentation] タウリンおよびシステイン、システアミン添加飼料がヒラメ稚魚の成長および遊離アミノ酸含量に及ぼす影響 (2018)
  • Author(s): 中村康平、MMGonzales、伊藤智子、益田玲爾、近藤秀裕、廣野育生、芳賀穣、佐藤秀一
  • Organizer: 平成30年度日本水産学会秋季大会
• [Presentation] Cystein dioxygenase and cysteamine dioxygenase as enzymes for turine synthesis and negative effect of cysteamine on growth of common carp Cypriuns carpio (2017)
  • Author(s): M.M.Gonzalez, Y.Haga, H.Kondo, I.Hirono, S.Satoh
  • Organizer: The JSFS 85th anniversary commemorative international symposium
  • Int'l Joint Research
• [Presentation] Influence of salinity and complete fish meal replacement on growth performance and relative expression of growth related genes in Nile tilapia, Oreochromis niloticus (2017)
  • Author(s): S.S.Herath, Y.Haga, M.Endo, S.Satoh
  • Organizer: The JSFS 85th anniversary commemorative international symposium
  • Int'l Joint Research
• [Presentation] Effects of chlorella supplementation to the low fish meal diets on growth, body composiion, digestion, and diesease resistance in rainbow trout, Oncorhnchus mykiss (2017)
  • Author(s): H.Mei, F.Li, Y.Haga, M.Endo, H.Kondo, I Hirono, S.Satoh
  • Organizer: The JSFS 85th anniversary commemorative international symposium
  • Int'l Joint Research
• [Presentation] Growth performance, survival, and expresssion of immune related gene in rainbow trout, Oncorhynchus mykiss pre-fed with supplemental level of arginine, ornithine, and citrulline upon challenge with Vibrio anguillarum (2017)
  • Author(s): I.A.Fauzi, Y.Haga, H.Kondo, I.Hirono, S.Satoh
  • Organizer: The JSFS 85th anniversary commemorative international symposium
  • Int'l Joint Research
• [Presentation] Non-fish meal, non-fish oil diet development for red seabream, Pagrus major, with microalgae Schizochytrium sp. (2017)
  • Author(s): T.Seong, Y.Haga, R.Kitagima, S.Satoh
  • Organizer: The JSFS 85th anniversary commemorative international symposium
  • Int'l Joint Research
• [Presentation] Effect of supplementation with taurine and enzyme comples to low fish meal diet on growth and enterohepatic health of juvenile red sea bream, Pagrus major (2017)
  • Author(s): F.Li, Y.Haga, H.Kondo, M.Endo, I.Hirono, S.Satoh
  • Organizer: The JSFS 85th anniversary commemorative international symposium
  • Int'l Joint Research
• [Presentation] First step of non-fish mea, non-fish oil diet development for red seabream, Pagrus major with microalgae, Schizochytrium sp. (2016)
  • Author(s): T.K.Seong, H.Matsutani, Y.Haga, R.Kitajima, S.Satoh
  • Organizer: 平成28年度日本水産学会秋季大会
  • Place of Presentation: 奈良市
  • Year and Date: 2016-09-08
• [Presentation] Evaluation of growth performance, plasma amino acid, and expression of arginine related genes in Oncorhynchus mykiss fed by different dietary arginine suppplementation level (2016)
  • Author(s): I.A.Fauzi, Y.Haga, H.Kondo, I.Hirono, S.Satoh
  • Organizer: 平成28年度日本水産学会秋季大会
  • Place of Presentation: 奈良市
  • Year and Date: 2016-09-08
• [Presentation] Isolation, molecular charactaerization, and tissue distribution of taurine synthesizing enzymes in Japanese flounder(Paralicthys olivaceus) (2016)
  • Author(s): M.M.Gonzales, Y.Haga, S.Satoh
  • Organizer: International Symposium on Fish Nutrition and Feeding 2016
  • Place of Presentation: San Valley, 米国
  • Year and Date: 2016-06-06
  • Int'l Joint Research
• [Presentation] Reduced salinity enhances growth and lipid levels but has no major effect on expression of taurine synthetic enzymes-like genes in Japanese flounder Paralicthys oliveceus (2016)
  • Author(s): Y.Haga, T.Itoh, H.Kondo, I.Hirono, R.Masuda, S.Satoh
  • Organizer: International Symposium on Fish Nutrition and Feeding 2016
  • Place of Presentation: San Valley, 米国
  • Year and Date: 2016-06-06
  • Int'l Joint Research
• [Presentation] Influence of tatal fishmeal replacement and salinity on growth performances and nutritional quality of Nile tilapia, Oreochromis niloticus (2016)
  • Author(s): S.Sakunthala, Y.Haga, M.Endo, S.Satoh
  • Organizer: International Symposium on Fish Nutrition and Feeding 2016
  • Place of Presentation: San Valley, 米国
  • Year and Date: 2016-06-06
  • Int'l Joint Research
• [Presentation] Combined effects of fishmeal replacement and salinity on growth performance of Nile tiapia, Oreochromis niloticus (2015)
  • Author(s): S.S.Herath,Y.Haga, M.Endo, S.Satoh
  • Organizer: 平成２７年日本水産学会秋季大会
  • Place of Presentation: 仙台
  • Year and Date: 2015-09-22
• [Presentation] Potential applicaton of corn co-prducts tofrmulate zero fish meal diets for juvenile Nile tilapia (Oreochromis niloticus) (2015)
  • Author(s): S.S.Herath, Y.Haga, M.Endo, S.Satoh
  • Organizer: Aquaculture 2015
  • Place of Presentation: Montpellier, France
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] Development of non-fish meal feed for red sea bream Pagrus major using with altenative feed stocks (2015)
  • Author(s): K.Matsukura, K.Hattori, Y.Haga, O.Nishizawa, S.Satoh
  • Organizer: Aquaculture 2015
  • Place of Presentation: Montpellier, France
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] Nutritional regultion of expression ofDHA and taurine synthesizing enzymes in Japanese flounder Paralichthys olivceus (2015)
  • Author(s): Y.Haga, H.Kondo, T.Itoh, R.Masuda, N.Kabeya, G.Yoshizaki, I.Hirono, S.Satoh
  • Organizer: Aquaculture 2015
  • Place of Presentation: Montpellier, France
  • Year and Date: 2015-08-23
  • Int'l Joint Research
• [Presentation] Impacts of different corn co-products on fillet color and growth performance of nile tilapia Oreochromis niloticus (2015)
  • Author(s): S.S.Herath, Y.Haga, S.Satoh
  • Organizer: World Aquaculture 2015
  • Place of Presentation: Cheju, Korea
  • Year and Date: 2015-05-25
  • Int'l Joint Research
• [Presentation] Does corn by-products chage fillet quality of Nile tilapia, Oreochromis niloticus? (2015)
  • Author(s): S.S.Herath, Y.Haga, M.Endo, S.Satoh
  • Organizer: 平成27年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
  • Year and Date: 2015-03-27 – 2015-03-31
• [Presentation] 異なる魚粉代替タンパク原料を組み合わせた無魚粉飼料がマダイの飼育成績および血漿化学組成に及ぼす影響 (2015)
  • Author(s): 松倉一樹、服部広大、芳賀穣、西澤央騎、輿石友彦、佐藤秀一
  • Organizer: 平成27年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学品川キャンパス
  • Year and Date: 2015-03-27 – 2015-03-31
• [Presentation] Development of non fish meal extruded pellet for Japanese yellowtail Seriola quinqeradiata (2014)
  • Author(s): S.Satoh, T.Tsuzuki, K.oshida, T.Hotta, M.Nakagawa, MYamada, T.Koshiishi, N.Ishida
  • Organizer: Aquaculture Europe 2014
  • Place of Presentation: San Sebastian Convention Ceter, Spain
  • Year and Date: 2014-10-14 – 2014-10-17
• [Presentation] Replacement of fish meal with rendered animal protein and plant protein sources on growth response, biological indices and amino acid availability of rainbow trout (Oncorhynchus mykiss) (2014)
  • Author(s): F.Lu, S.Satoh, Y.Haga
  • Organizer: The 16th International Symposium on Fish Nutrition and Feeing
  • Place of Presentation: Cairns Convention Centre, Australia
  • Year and Date: 2014-05-25 – 2014-05-30