Molecular mechanism mediating estrogen feedback to regulate follicular development and ovulation in domestic animals

• Project/Area Number: 26252046
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal production science
• Research Institution: Nagoya University
• Principal Investigator: Tsukamura Hiroko 名古屋大学, 生命農学研究科, 教授 (00212051)
• Co-Investigator(Kenkyū-buntansha): 大蔵 聡 名古屋大学, 生命農学研究科, 教授 (20263163) ; 平林 真澄 生理学研究所, 行動・代謝分子解析センター, 准教授 (20353435) ; 前多 敬一郎 東京大学, 大学院農学生命科学研究科(農学部), 教授 (30181580)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥38,610,000 (Direct Cost: ¥29,700,000、Indirect Cost: ¥8,910,000); Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2015: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2014: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
• Keywords: キスペプチン / GPR54 / GnRH / メタスチン / エストロゲン

Outline of Final Research Achievements

The present study aimed to investigate the mechanism mediating estrogen negative and positive feedback regulating follicular development and ovulation, respectively, in mammals. The study focused on the effects of estrogen on Kiss1 (kisspeptin gene) expression in kisspeptin neurons, and demonstrates that the arcuate (ARC) and anteroventral paraventricular nucleus (AVPV)/ preoptic kisspeptin neurons are targets of estrogen to exert the estrogen negative and positive feedback effects, respectively, in rats, mouse, monkeys, and goats. The study also revealed that specific transcriptional factors are expressed in the both kisspeptin neuronal populations. Further, the present study tried to generate immortalized rat AVPV and ARC kisspeptin neuronal cell lines to investigate molecular mechanism mediating the estrogen effects. As a result, we obtained some kisspeptin neuronal cell line candidates from the AVPV and ARC.

Research Products

• [Journal Article] Evaluation of heat stress response in crossbred dairy cows under tropical climate by analysis of heart rate variability (2018)
  • Author(s): Bun, C., Watanabe, Y., Uenoyama, Y., Inoue, N., Ieda, N., Matsuda, F., Tsukamura, H., Kuwahara, M., Maeda, K.-I., Ohkura, S. and Pheng, V.
  • Journal Title: Journal of Veterinary Medical Science Volume: 80 Issue: 1 Pages: 181-185
  • DOI: 10.1292/jvms.17-0368
  • NAID: 130006331058
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Co‐expression of the calcitonin receptor gene in the hypothalamic kisspeptin neurons in female rats. (2018)
  • Author(s): Assadullah, Nahoko Ieda, Narumi Kawai, Hirotaka Ishii, Kunio Ihara, Naoko Inoue, Yoshihisa Uenoyam, Hiroko Tsukamura
  • Journal Title: Reprod Med Biol. Volume: 17 Issue: 2 Pages: 164-172
  • DOI: 10.1002/rmb2.12085
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fetal/perinatal programming causing sexual dimorphism of the kisspeptin-GnRH neuronal network (2018)
  • Author(s): Tsukamura H, Maeda KI, Uenoyama Y.
  • Journal Title: In: Herbison AE, Plant TM, eds. The GnRH Neuron and its Control: Wiley Volume: 印刷中
• [Journal Article] CRISPR/Cas9-mediated genome editing in wild-derived mice: generation of tamed wild-derived strains by mutation of the a (nonagouti) gene (2017)
  • Author(s): Michiko Hirose, Ayumi Hasegawa, Keiji Mochida, Shogo Matoba, Yuki Hatanaka, Kimiko Inoue, Tatsuhiko Goto, Hideki Kaneda, Ikuko Yamada, Tamio Furuse, Kuniya Abe, Yoshihisa Uenoyama, Hiroko Tsukamura, Shigeharu Wakana, Arata Honda, and Atsuo Ogura
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 42476-42476
  • DOI: 10.1038/srep42476
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Long -term neonatal estrogen exposure causes irreversible inhibition of LH pulses by suppressing arcuate kisspeptin expression via estrogen receptors α and β in female rodents. (2017)
  • Author(s): Minabe S., Ieda N., Watanabe Y., Inoue, N., Uenoyama Y., Maeda K.-I., and Tsukamura H.
  • Journal Title: Endocrinology Volume: 158(6) Issue: 9 Pages: 1-12
  • DOI: 10.1210/en.2016-1144
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Evidence of involvement of neurone-glia/neurone-neurone communications via gap junctions in synchronised activity of KNDy neurones (2017)
  • Author(s): Ikegami K.、Minabe S.、Ieda N.、Goto T.、Sugimoto A.、Nakamura S.、Inoue N.、Oishi S.、Maturana A. D.、Sanbo M.、Hirabayashi M.、Maeda K.-I.、Tsukamura H.、Uenoyama Y.
  • Journal Title: Journal of Neuroendocrinology Volume: 29 Issue: 6
  • DOI: 10.1111/jne.12480
  • Peer Reviewed
• [Journal Article] Enhancement of the LH surge by male olfactory signals is associated with AVPV Kiss1 cell activation in female rats. (2017)
  • Author(s): Watanabe Y, Ikegami K, Ishigaki R, Ieda N, Uenoyama Y, Maeda KI, Tsukamura H. Inoue N.
  • Journal Title: J Neuroendocrinol Volume: - Issue: 8 Pages: 1-9
  • DOI: 10.1111/jne.12505
  • Peer Reviewed
• [Journal Article] SB223412, a neurokinin-3 receptor-selective antagonist, suppresses testosterone secretion in male guinea pigs (2017)
  • Author(s): Nakamura Sho、Ito Yoshiko、Yamamoto Koki、Takahashi Chudai、Dai Mingdao、Tanahashi Miyu、Uenoyama Yoshihisa、Tsukamura Hiroko、Oishi Shinya、Maeda Kei-ichiro、Matsuda Fuko
  • Journal Title: Theriogenology Volume: 102 Pages: 183-189
  • DOI: 10.1016/j.theriogenology.2017.07.053
  • Peer Reviewed
• [Journal Article] キスペプチンによる生殖制御機構 (2017)
  • Author(s): 家田菜穂子・井上直子・束村博子・上野山賀久
  • Journal Title: 内分泌・糖尿病・代謝内科 Volume: 44 Pages: 2-6
• [Journal Article] 哺乳類の生殖制御メカニズムとその老化 (2017)
  • Author(s): 上野山賀久・井上直子・束村博子
  • Journal Title: 日本抗加齢医学会雑誌 Volume: 13 Pages: 808-812
• [Journal Article] Immunohistochemical characterization of the arcuate kisspeptin/neurokinin B/dynorphin (KNDy) and preoptic kisspeptin neuronal populations in the hypothalamus during the estrous cycle in heifers. (2016)
  • Author(s): Hassaneen ASA, Naniwa Y, Suetomi Y, Matsuyama S, Kimura K, Ieda N, Inoue N, Uenoyama Y, Tsukamura H, Maeda K-I, Matsuda F, Ohkura S.
  • Journal Title: J. Reprod. Dev. Volume: 62(5) Pages: 471-477
  • NAID: 130005431286
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Neonatal kisspeptin is steroid-independently required for defeminisation and peripubertal kisspeptin-induced testosterone is required for masculinisation of the brain: A behavioural study using Kiss1 KO rats. (2016)
  • Author(s): Nakamura, S., Uenoyama, Y., Ikegami, K., Dai, M., Watanabe, Y., Takahashi, C., Hirabayashi, M., Tsukamura, H. and Maeda, K.-I.
  • Journal Title: J. Neuroendocrinol. Volume: 999 Issue: 10 Pages: 999-999
  • DOI: 10.1111/jne.12409
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ほ乳類の性成熟を制御する脳内メカニズムの解明 (2016)
  • Author(s): 上野山賀久・束村博子
  • Journal Title: 生物科学 Volume: 67 Pages: 161-167
  • Peer Reviewed
• [Journal Article] Molecular and Epigenetic Mechanism Regulating Hypothalamic Kiss1 Gene Expression in Mammals. (2016)
  • Author(s): Uenoyama Y, Tomikawa J, Inoue N, Goto T, Minabe S, Ieda N, Nakamura S, Watanabe Y, Ikegami K, Matsuda F, Ohkura S, Maeda KI, Tsukamura H.
  • Journal Title: Neuroendocrinology Volume: 印刷中 Issue: 6 Pages: 640-649
  • DOI: 10.1159/000445207
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Maturation and physiology of hypothalamic regulation of the gonadal axis. (2016)
  • Author(s): Uenoyama, Y., Inoue, N., Ieda, N., Pheng, V., Maeda, K.-I. and Tsukamura, H.
  • Journal Title: Springer Volume: 999 Pages: 1-11
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The roles of kisspeptin revisited: inside and outside the hypothalamus. (2016)
  • Author(s): Uenoyama, Y., Pheng, V., Tsukamura, H. and Maeda, K.-I.
  • Journal Title: J. Reprod. Dev. Volume: 62 Pages: 537-545
  • NAID: 130005251168
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] ほ乳類の生殖機能を制御するエストロジェンのフィードバック作用を仲介する脳内メカニズム (2016)
  • Author(s): 上野山賀久、束村博子
  • Journal Title: 日本生殖内分泌学会雑誌 Volume: 21 Pages: 5-8
  • NAID: 40020952331
  • Acknowledgement Compliant
• [Journal Article] 視床下部による卵胞発育制御機構～卵胞発育を理解する～知っておくべき基礎知識【卵胞発育の生理】 (2016)
  • Author(s): 井上直子、束村博子、上野山賀久
  • Journal Title: 臨床婦人科産科 Volume: 70 Pages: 1094-1097
• [Journal Article] ほ乳類の性成熟を制御する脳内メカニズムの解明 (2016)
  • Author(s): 上野山賀久, 束村博子
  • Journal Title: 生物科学 Volume: 第67巻3号 Pages: 1-7
  • Peer Reviewed / Open Access
• [Journal Article] Pharmacological and morphological evidence of AMPK-mediated energy sensing in the lower brain stem ependymocytes to control reproduction in female rodents. (2015)
  • Author(s): Minabe, S., Deura, C., Ikegami, K., Goto, T., Sanbo, M., Hirabayashi, M., Inoue, N., Uenoyama, Y., Maeda, K., Tsukamura, H.
  • Journal Title: Endocrinology Volume: in press Issue: 6 Pages: 2278-2287
  • DOI: 10.1210/en.2014-2018
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Central estrogen action sites involved in prepubertal restraint of pulsatile luteinizing hormone release in female rats (2015)
  • Author(s): Uenoyama Y, Tanaka A, Takase K, Yamada S, Pheng V, Inoue N, Maeda K, Tsukamura H.
  • Journal Title: Journal of Reproduction and Development Volume: 61 Issue: 4 Pages: 351-359
  • DOI: 10.1262/jrd.2014-143
  • NAID: 130005094644
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Luteinising Hormone Surge-Generating System is Functional in Male Goats as in Females: Involvement of Kisspetin Neurons in the Medial Preoptic Area (2015)
  • Author(s): F. Matsuda, K. Nakatsukasa, Y. Suetomi, Y. Naniwa, D. Ito, N. Inoue, Y. Wakabayashi, H. Okamura, K.-I. Maeda, Y. Uenoyama, H. Tsukamura and S. Ohkura
  • Journal Title: Journal of Neuroendocrinology Volume: 27 Issue: 1 Pages: 57-65
  • DOI: 10.1111/jne.12235
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification of hypothalamic arcuate nucleus-specific enhancer region of kiss1 gene in mice. (2015)
  • Author(s): Goto, T., Tomikawa, J., Ikegami, K., Minabe, S., Abe, H., Fukanuma, T., Imamura, T., Takase, K., Sanbo, M., Tomita, K., Hirabayashi, M., Maeda, K., Tsukamura, H., Uenoyama, Y.
  • Journal Title: Mol Endocrinol Volume: 29 Issue: 1 Pages: 121-129
  • DOI: 10.1210/me.2014-1289
  • Peer Reviewed / Open Access
• [Journal Article] Lack of pulse and surge modes and glutamatergic stimulation of LH release in Kiss1 knockout rats. (2015)
  • Author(s): Uenoyama, Y., Nakamura, S., Hayakawa, Y., Ikegami, K., Watanabe, Y., Deura, C., Minabe, S., Tomikawa, J., Goto, T., Ieda, N., Inoue, N., Sanbo, M., Tamura, C., Hirabayashi, M., Maeda, K., Tsukamura, H.
  • Journal Title: J Neuroendocrinol Volume: 27 Issue: 3 Pages: 187-197
  • DOI: 10.1111/jne.12257
  • Peer Reviewed / Open Access
• [Journal Article] Design and synthesis of fluorescent probes for GPR54. (2014)
  • Author(s): Kaneda M, Misu R, Ohno H, Hirasawa A, Ieda N, Uenoyama Y, Tsukamura H, Maeda K, Oishi S, Fujii N.
  • Journal Title: Bioorg. Med. Chem. Volume: 22 Issue: 13 Pages: 3325-3330
  • DOI: 10.1016/j.bmc.2014.04.052
  • Peer Reviewed
• [Journal Article] <i>KISS1</i> Gene Expression in the Developing Brain of Female Pigs in Pre- and Peripubertal Periods (2014)
  • Author(s): Ieda, N., Uenoyama, Y., Tajima, Y., Nakata, T., Kano, M., Naniwa, Y., Watanabe, Y., Minabe, S., Tomikawa, J., Inoue, N., Matsuda, F., Ohkura, S., Maeda, K.-I. and Tsukamura, H.
  • Journal Title: Journal of Reproduction and Development Volume: 60 Issue: 4 Pages: 312-316
  • DOI: 10.1262/jrd.2013-129
  • NAID: 130004137827
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] Oestrogen-Induced Activation of Preoptic Kisspeptin Neurones May be Involved in the Luteinising Hormone Surge in Male and Female Japanese Monkeys (2014)
  • Author(s): Watanabe Y, Uenoyama Y, Suzuki J, Takase K, Suetomi Y, Ohkura S, Inoue N, Maeda KI. and Tsukamura H
  • Journal Title: Journal of Neuroendocrinology Volume: 26 Issue: 12 Pages: 909-917
  • DOI: 10.1111/jne.12227
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 新たな中枢性繁殖制御剤のシーズ：KNDyニューロン (2014)
  • Author(s): 前多敬一郎・大石真也・束村博子・上野山賀久・大蔵聡・松田二子
  • Journal Title: MPアグロジャーナル Volume: 16 Pages: 17-21
  • NAID: 40020266615
• [Journal Article] KNDy neuron as a gatekeeper of puberty onset. (2014)
  • Author(s): Uenoyama, Y. Tsukamura, H., Maeda, K.
  • Journal Title: J Obstet Gynacol Res. Volume: 40 Issue: 6 Pages: 1518-1526
  • DOI: 10.1111/jog.12398
  • Peer Reviewed
• [Presentation] The Tracing Study of the Neural Pathway Originating from the Hindbrain Ependymocytes for Regulating Reproductive Functions (2018)
  • Author(s): Kei Horihata, Naoko Inoue, Nahoko Ieda, Yoshihisa Uenoyama, Yuta Suetomi, Fuko Matsuda, Kei-ichiro Maeda and Hiroko Tsukamura
  • Organizer: ENDO 2018
  • Int'l Joint Research
• [Presentation] Establishment of Immortalized Cell Lines Derived from Rat AVPV and ARC Kisspeptin Neurons (2018)
  • Author(s): Chikaya Deura, Naoko Inoue, Yoshihisa Uenoyama, Kei-ichiro Maeda and Hiroko Tsukamura
  • Organizer: ENDO 2018
  • Int'l Joint Research
• [Presentation] GnRHパルスおよびサージ発生中枢の同定を目的としたKiss1-floxedラットの作出 (2018)
  • Author(s): 長江麻佑子、余郷享子、後藤哲平、三宝誠、平林真澄、井上直子、束村博子、前多敬一郎、上野山賀久
  • Organizer: 日本畜産学会第124回大会
• [Presentation] 哺乳類の生殖を制御する神経内分泌機構―キスペプチニューロンを中心にー (2017)
  • Author(s): 束村博子
  • Organizer: 日本内分泌学会学術総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2017-04-21
  • Invited
• [Presentation] Involvement of gap junctional communications between KNDy neurons and glial cells in synchronized discharges of KNDy neurons in mice. (2017)
  • Author(s): Kana Ikegami, Nahoko Ieda, Teppei Goto, Alisa Sugimoto, Sho Nakamura, Naoko Inoue, Kei-ichiro Maeda, Hiroko Tsukamura and Yoshihisa Uenoyama
  • Organizer: 3rd World Conference of Kisspeptin-Brain and Beyond
  • Place of Presentation: Orland, USA
  • Year and Date: 2017-03-30
  • Int'l Joint Research
• [Presentation] Development/Sex Differences of Kiss1 neurons. (2017)
  • Author(s): Tsukamura, H.
  • Organizer: 3rd World Conference of Kisspeptin-Brain and Beyond
  • Place of Presentation: Orland, USA
  • Year and Date: 2017-03-30
  • Int'l Joint Research / Invited
• [Presentation] 前腹側室周囲核へのATPの投与がLH分泌に及ぼす影響 (2017)
  • Author(s): 石垣蓮、家田菜穂子、上野山賀久、束村博子、井上直子
  • Organizer: 畜産学会第122回大会
  • Place of Presentation: 神戸大学鶴甲第１キャンパス（神戸市）
  • Year and Date: 2017-03-26
• [Presentation] 生殖を制御する中枢メカニズム：キスペプチンとその発現のエピジェネティック機構 (2017)
  • Author(s): 束村博子
  • Organizer: 第９０回日本内分泌学会学術総会
  • Invited
• [Presentation] 雌ラットKNDyニューロンにおけるGnRH代謝産物受容体の発現 (2017)
  • Author(s): 家田菜穂子、井上直子、上野山賀久、束村博子
  • Organizer: 第14回GPCR研究会
• [Presentation] 哺乳類におけるHPG軸と生殖機能制御 (2017)
  • Author(s): 井上直子、家田菜穂子、上野山賀久、束村博子
  • Organizer: 第35回内分泌代謝学サマーセミナー
• [Presentation] 雌由来臭覚刺激は雌ラット前腹側室周囲核キスペプチンニューロンを活性化しLH分泌を増強する (2017)
  • Author(s): 渡辺雄貴、池上花奈、石垣蓮、家田菜穂子、上野山賀久、前多敬一郎、束村博子、井上直子
  • Organizer: 第35回内分泌代謝学サマーセミナー
• [Presentation] Histological analysis of the possible participation of TIP39 and somatostatin in Kiss1 suppression during lactation (2017)
  • Author(s): Alisa Sugimoto, Yoshihisa Uenoyama, Nohoko Ieda, Kana Ikegami, Naoko Inoue and Hiroko Tsukamura
  • Organizer: 4th World Congress of Reproductive Biology
  • Int'l Joint Research
• [Presentation] Establishment and evaluation of rat kisspeptin neuronal cell lines (2017)
  • Author(s): Kei Horihata, Ai Takahashi, Naoko Inoue, Nahoko Ieda, Yoshihisa Uenoyama, Yuta Suetomi, Fuko Matsuda, Kei-ichiro Maeda and Hiroko Tsukamura
  • Organizer: 4th World Congress of Reproductive Biology
  • Int'l Joint Research
• [Presentation] The Neural pathway originating form the ependymocytes of the hindbrain to the kisspeptin neurons (2017)
  • Author(s): Chikaya Deura, Naoko Inoue, Kei-ichiro Maeda, Yoshihisa Uenoyama and Hiroko Tsukamura
  • Organizer: 4th World Congress of Reproductive Biology
  • Int'l Joint Research
• [Presentation] The laclization of μ-opioid receptor-expressing cells in the female rat brain (2017)
  • Author(s): Narumi Kawai, Nahoko Ieda, Yoshihisa Uenoyama, Naoko Inoue and Hiroko Tsukamura
  • Organizer: 4th World Congress of Reproductive Biology
  • Int'l Joint Research
• [Presentation] Expression of calcitonin receptors in kisspeptin neurons in the arcuate and anteroventral periventicular nuclei in female rats (2017)
  • Author(s): Assadullah Dost, Nahoko Ieda, Naoko Inoue, Yoshihisa Uenoyama and Hiroko Tsukamura
  • Organizer: 4th World Congress of Reproductive Biology
  • Int'l Joint Research
• [Presentation] The effect of administration of ATP into the anteroventral periventricular nucleus on LH secretion in female rats (2017)
  • Author(s): Ren Ishigaki, Nahoko Ieda, Yoshihisa Uenoyama, Hiroko Tsukamura and Naoko Inoue
  • Organizer: 4th World Congress of Reproductive Biology
  • Int'l Joint Research
• [Presentation] Expression of Gpr101, receptor gene for GnRH metabolite, in KNDy neurons in female rats (2017)
  • Author(s): Nahoko Ieda, Shiori Minabe, Kana Ikegami, Youki Watanabe, Alisa Sugimoto, Naoko Inoue, Yoshihisa Uenoyama, Kei-ishiro Maeda and Hiroko Tsukamura
  • Organizer: 4th World Congress of Reproductive Biology
  • Int'l Joint Research
• [Presentation] 生命現象をのぞき込む～試験に出ない？基礎研究の凄みと楽しさ～ (2017)
  • Author(s): 束村博子
  • Organizer: 第10回形態科学シンポジウム
  • Invited
• [Presentation] Suckling-induced changes in TIP39 and somatostatin expressions in the rat brain (2017)
  • Author(s): Alisa Sugimoto, Yoshihisa Uenoyama, Nahoko Ieda, Kana Ikegami, Naoko Inoue and Hiroko Tsukamura
  • Organizer: Society for Neuroscience
  • Int'l Joint Research
• [Presentation] 「内分泌学」のダイナミズム (2017)
  • Author(s): 束村博子
  • Organizer: 日本内分泌学会創立90周年記念式典
  • Invited
• [Presentation] 低栄養による哺乳類の生殖機能抑制の脳内メカニズム (2017)
  • Author(s): 束村博子
  • Organizer: 2017年度生命科学系学会合同年次大会
  • Invited
• [Presentation] 脳内 ATP-プリン受容体を介した排卵中枢制御メカニズム (2017)
  • Author(s): 石垣蓮・家田菜穂子・上野山賀久・束村博子・井上直子
  • Organizer: 平成29年度 東海畜産学会大会
• [Presentation] 卵胞発育中枢および排卵中枢の同定を目的とした Kiss1-floxed ラットの作出 (2017)
  • Author(s): 長江麻佑子・余郷享子・後藤哲平・三宝誠・平林真澄・ 井上直子・束村博子・前多敬一郎・上野山賀久
  • Organizer: 平成29年度 東海畜産学会大会
• [Presentation] 哺乳類の生殖中枢の制御機構解明に資するラットキスペプチンニューロン不死化細胞株の作出 (2017)
  • Author(s): 堀畑慶・井上直子・家田菜穂子・上野山賀久・末富祐太・松田二子・前多敬一郎・束村博子
  • Organizer: 平成29年度 東海畜産学会大会
• [Presentation] TIP39-ソマトスタチン系による泌乳期キスペプチン遺伝子発現抑制の可能性 (2017)
  • Author(s): 杉本有沙、上野山賀久、渡辺雄貴、家田菜穂子、池上花奈、井上直子、束村博子
  • Organizer: 第21回日本生殖内分泌学会学術集会
  • Place of Presentation: 千里ライフサイエンスセンター（大阪府豊中市）
• [Presentation] 性成熟期におけるKNDyニューロンの神経ペプチド遺伝子発現におよぼすエストロジェンの影響 (2016)
  • Author(s): 小林卓磨、上野山賀久、家田菜穂子、井上直子、束村博子
  • Organizer: 第43回日本神経内分泌学会学術集会
  • Place of Presentation: アクトシティ浜松（静岡県浜松市）
  • Year and Date: 2016-10-14
• [Presentation] ラット脳内におけるμ-オピオイド受容体発現細胞の局在 (2016)
  • Author(s): 河合成美、家田菜穂子、上野山賀久、井上直子、束村博子
  • Organizer: 第43回日本神経内分泌学会学術集会
  • Place of Presentation: アクトシティ浜松（静岡県浜松市）
  • Year and Date: 2016-10-14
• [Presentation] ラットキスペプチンニューロン不死化細胞株の樹立 (2016)
  • Author(s): 堀畑慶、家田菜穂子、井上直子、上野山賀久、末富祐太、松田二子、前多敬一郎、束村博子
  • Organizer: 第109回日本繁殖生物学会大会
  • Place of Presentation: 麻布大学（神奈川県相模原市）
  • Year and Date: 2016-09-11
• [Presentation] 吸乳刺激による弓状核Kiss1遺伝子発現抑制がTIP39-ソマトスタチンニューロン経路により仲介される可能性 (2016)
  • Author(s): 杉本有沙、上野山賀久、渡辺雄貴、家田菜穂子、池上花奈、井上直子、束村博子
  • Organizer: 第109回日本繁殖生物学会大会
  • Place of Presentation: 麻布大学（神奈川県相模原市）
  • Year and Date: 2016-09-11
• [Presentation] Cell-to-cell communication via gap junctions may play a role in gonadotropin-releasing hormone pulse generation. (2016)
  • Author(s): Ikegami, K., Ieda, N., Minabe, S., Goto, T., Inoue, N., Maeda, K.-I., Tsukamura H., and Yoshihisa Uenoyama
  • Organizer: International Symposium on Pituitary Gland and Related Systems (ISPGRS 2016)
  • Place of Presentation: East-West Center, University of Hawaii
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] Establishment of rat kisspeptin neuronal cell lines. (2016)
  • Author(s): Kei Horihata, Nahoko Ieda, Noko Inoue, Yoshihisa Uenoyama, Yuta Suetomi, Fuko Matsuda, Kei-ichiro Maeda, Hiroko Tsukamura
  • Organizer: International Symposium on Pituitary Gland and Related Systems (ISPGRS 2016)
  • Place of Presentation: East-West Center, University of Hawaii
  • Year and Date: 2016-09-01
  • Int'l Joint Research
• [Presentation] 脳の性分化研究からみる男女共同参画 (2016)
  • Author(s): 束村博子
  • Organizer: 第34回内分泌代謝学サマーセミナー
  • Place of Presentation: 久山温泉 ホテル夢家
  • Year and Date: 2016-07-14
  • Invited
• [Presentation] 排卵中枢キスペプチンニューロンの性分化には種差がある (2016)
  • Author(s): 松田二子、渡辺雄貴、大蔵聡、井上直子、上野山賀久、前多敬一郎、束村博子
  • Organizer: 第13回GPCR研究会
  • Place of Presentation: 日本科学未来館
  • Year and Date: 2016-05-13
• [Presentation] WCRBの日本開催と日本の家畜繁殖学 (2016)
  • Author(s): 束村博子
  • Organizer: 畜産学会第121回大会
  • Place of Presentation: 日本獣医生命科学大学
  • Year and Date: 2016-03-27
  • Invited
• [Presentation] 哺乳類の生殖を制御するキスペプチンーGPCR54シグナリングの役割 (2016)
  • Author(s): 束村博子、上野山賀久
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-22
  • Invited
• [Presentation] 性腺機能低下症の基礎と臨床:最新のトピックス 「ほ乳類の生殖機能を制御するキスペプチンニューロンの役割」 (2016)
  • Author(s): 束村博子
  • Organizer: 第20回日本生殖内分泌学会学術集会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2016-01-09
  • Invited
• [Presentation] GnRH ニューロンからキスペプチンニューロンへのポジティブ・ショートループ・フィードバック機構 (2016)
  • Author(s): 家田菜穂子、美辺詩織、池上花奈、井上直子、上野山賀久、前多敬一郎、束村博子
  • Organizer: 第20回日本生殖内分泌学会学術集会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2016-01-09
• [Presentation] Kisspeptin and Reproduction. (2016)
  • Author(s): Tsukamura, H.
  • Organizer: China Agricultral University Seminar
  • Place of Presentation: China Agricultral University
  • Int'l Joint Research / Invited
• [Presentation] Involvement of Preoptic Kisspeptin Neurons in Estrogen Positive Feedback to induce Luteinizing Hormone surge in both female and male Japanese monkey (2015)
  • Author(s): Y. Watanabe, Y. Uenoyama, J. Suzuki, K. Takase, Y. Suetomi, S. Ohkura, N. Inoue, K.-I. Maeda and H. Tsukamura.
  • Organizer: International Conference on Biology and Pathology of Reproduction in Domestic Animals
  • Place of Presentation: グダニスク (ポーランド)
  • Year and Date: 2015-09-28
  • Int'l Joint Research
• [Presentation] : Involvement of cell-to-cell communication via gap junctions in NKB-NK3R signaling-induced synchronous discharges of KNDy neurons. (2015)
  • Author(s): K. Ikegami, S. Minabe, N. Ieda, T. Goto, S. Nakamura, K. Maeda, H. Tsukamura and Y. Uenoyama
  • Organizer: International Conference on Biology and Pathology of Reproduction in Domestic Animals
  • Place of Presentation: グダニスク (ポーランド)
  • Year and Date: 2015-09-28
  • Int'l Joint Research
• [Presentation] コンディショナルKiss1KOマウスを用いたGnRHパルス発生機構の同定とKiss1可視化マウス in vitro系によるパルス発生分子メカニズムの解明 (2015)
  • Author(s): 池上花奈、美辺詩織、家田菜穂子、渡辺雄貴、後藤哲平、中村翔、井上直子、前多敬一郎、束村博子、上野山賀久
  • Organizer: 第108回日本繁殖生物学会大会
  • Place of Presentation: 宮崎大学木花キャンパス
  • Year and Date: 2015-09-17
• [Presentation] メスとの同居はオスラットAVPVのKiss1発現およびLH分泌上昇を誘起する (2015)
  • Author(s): 渡辺雄貴、井上直子、池上花奈、上野山賀久、束村博子
  • Organizer: 第108回日本繁殖生物学会大会
  • Place of Presentation: 宮崎大学木花キャンパス
  • Year and Date: 2015-09-17
• [Presentation] キスペプチン・GnRHニューロン間のultra-short loop feedbackによるLH分泌促進機構 (2015)
  • Author(s): 家田菜穂子、美辺詩織、池上花奈、井上直子、上野山賀久、前多敬一郎、束村博子
  • Organizer: 第108回日本繁殖生物学会大会
  • Place of Presentation: 宮崎大学木花キャンパス
  • Year and Date: 2015-09-17
• [Presentation] オスラットの床敷曝露によるメスラット前腹側室周囲核(AVPV)キスペプチンニューロンの活性化およびLH分泌の増強作用 (2015)
  • Author(s): 渡辺雄貴、井上直子、池上花奈、上野山賀久、束村博子
  • Organizer: 第30回日本下垂体研究会学術集会
  • Place of Presentation: 黒部市宇奈月国際会館セレネ
  • Year and Date: 2015-08-05
• [Presentation] 性行動中枢の雄性化／脱雄性化におけるキスペプチンの役割 (2015)
  • Author(s): 中村翔、上野山賀久、池上花奈、載明道、高橋宙大、平林真澄、束村博子、前多敬一郎
  • Organizer: 第30回日本下垂体研究会学術集会
  • Place of Presentation: 黒部市宇奈月国際会館セレネ
  • Year and Date: 2015-08-05
• [Presentation] 性腺刺激ホルモン放出ホルモン(GnRH)のパルス状分泌に関与するkappa opioid receptor(KOR) 発現細胞の局在解明 (2015)
  • Author(s): 高橋宙大、中村翔、載明道、後藤哲平、平林真澄、上野山賀久、束村博子、前多敬一郎
  • Organizer: 第30回日本下垂体研究会学術集会
  • Place of Presentation: 黒部市宇奈月国際会館セレネ
  • Year and Date: 2015-08-05
• [Presentation] キスペプチンニューロンによる生殖機能制御メカニズム (2015)
  • Author(s): 束村博子
  • Organizer: 第19回小児分子内分泌研究会
  • Place of Presentation: 大沼国際セミナーハウス
  • Year and Date: 2015-07-05
  • Invited
• [Presentation] 生殖を制御する脳内メカニズム～キスペプチンニューロンを中心に～ (2015)
  • Author(s): 束村博子
  • Organizer: 第37回中部生殖医学会学術集会
  • Place of Presentation: 名古屋大学医学部鶴舞キャンパス
  • Year and Date: 2015-06-06
  • Invited
• [Presentation] 視床下部による生殖機能制御メカニズムーキスペプチンニューロンを中心にー (2015)
  • Author(s): 束村博子
  • Organizer: 第88回日本内分泌学会学術総会
  • Place of Presentation: ホテルニューオータニ東京
  • Year and Date: 2015-04-23
  • Invited
• [Presentation] Kiss1ノックアウト雄ラットにおける生殖機能不全 (2015)
  • Author(s): 上野山賀久、中村翔、池上花奈、美辺詩織、家田菜穂子、冨川順子、後藤哲平、前多敬一郎、束村博子
  • Organizer: 第88回日本内分泌学会学術総会
  • Place of Presentation: ホテルニューオータニ東京
  • Year and Date: 2015-04-23
• [Presentation] 性ステロイドフィードバックと脳内プログラミング (2015)
  • Author(s): 束村博子
  • Organizer: 第１２０回日本解剖学会総会・全国学術集会・第９２回日本生理学会大会
  • Place of Presentation: 神戸国際会議場（神戸市中央区）
  • Year and Date: 2015-03-21 – 2015-03-23
  • Invited
• [Presentation] Stimulation of LH secretion By Ultra-Shortloop Positive Feedback Between GnRH and Kisspeptin Neurons (2015)
  • Author(s): Ieda, N., Minabe, S., Ikegami, K., Sugimoto, Y., Sugimoto, A., Inoue, N., Uenoyama, Y., Maeda K.-I., Tsukamura H.
  • Organizer: ENDO2015
  • Place of Presentation: San Diego Convention Center San Diego, CA(USA)
  • Year and Date: 2015-03-05 – 2015-03-08
• [Presentation] GnRH部分ペプチドGnRH(1-5)は弓状核キスペプチン(KNDy)ニューロンを介してLH分泌を促進する (2015)
  • Author(s): 家田菜穂子、美辺詩織、井上直子、上野山賀久、前多敬一郎、束村博子
  • Organizer: 第１９回日本生殖内分泌学会学術集会
  • Place of Presentation: 千里ライフサイエンスセンター（大阪府豊中市）
  • Year and Date: 2015-01-10
• [Presentation] 「種を超えてほ乳類の生殖機能を制御するキスペプチンニューロン」 (2014)
  • Author(s): 束村博子
  • Organizer: 第41回日本神経内分泌学会学術集会
  • Place of Presentation: 都道府県会館（東京都千代田区）
  • Year and Date: 2014-10-31 – 2014-11-02
  • Invited
• [Presentation] Kisspeptin-GPR54 signaling controlling reproductive functions in mammals (2014)
  • Author(s): Tsukamura, H.
  • Organizer: World Congress of Reproductive Biology 2014
  • Place of Presentation: Edinburgh (Scotland)
  • Year and Date: 2014-09-02 – 2014-09-04
  • Invited
• [Presentation] Establishment of immortalized neuronal cell lines derived from fetal goat hypothalamus. (2014)
  • Author(s): Suetomi, Y., Tsukamura, H., Ohkura, S., Matsuda, F.
  • Organizer: World Congress of Reproductive Biology 2014
  • Place of Presentation: Edinburgh(Scotland)
  • Year and Date: 2014-09-02 – 2014-09-04
• [Presentation] Changes in gene expressions induced by perinatal estrogen related to the brain sexual differentiation in rodents (2014)
  • Author(s): Watanabe, Y., Sakakibara, M., Uenoyama, Y., Minabe, S., Deura, C., Nakamura, S., Maeda, K.-I., Tsukamura, H.
  • Organizer: World Congress of Reproductive Biology 2014
  • Place of Presentation: Edinburgh(Scotland)
  • Year and Date: 2014-09-02 – 2014-09-04
• [Presentation] Neurokinin B activates synchronized intracellular Ca2+ oscillations in KNDy neurons obtained from the hypothalamic arcuate nucleus of Kiss1-GFP transgenic mice (2014)
  • Author(s): Ikegami, K., Minabe, S., Ieda, N., Goto, T., Abe, H., Sanbo, M., Hirabayashi, M., Maeda, K.-I., Tsukamura, H., Uenoyama Y.
  • Organizer: World Congress of Reproductive Biology 2014
  • Place of Presentation: Edinburgh(Scotland)
  • Year and Date: 2014-09-02 – 2014-09-04
• [Presentation] Hypothalamic arcuate nuleus-specific enhancer for kisspeptin expression of female mice (2014)
  • Author(s): Goto, T., Tsukamura, H., Tomikawa, J., Abe, H., Fukanuma, T., Imaura, T., Takase, K., Sanbo, M., Tomita, K., Hirabayashi, M., Maeda, K.-I., Uenoyama Y.
  • Organizer: World Congress of Reproductive Biology 2014
  • Place of Presentation: Edinburgh(Scotland)
  • Year and Date: 2014-09-02 – 2014-09-04
• [Presentation] Lack of gonadotropin release in Kiss1 knockout male rats (2014)
  • Author(s): Uenoyama, Y., Nakamura, S., Hayakawa, Y., Ikegami, K., Deura, C., Minabe, S., Tomikawa, J., Goto, T., Ieda, N., Sanbo, M., Tamura, C., Hirabayashi, M., Maeda, K.-I., Tsukamura H.
  • Organizer: World Congress of Reproductive Biology 2014
  • Place of Presentation: Edinburgh(Scotland)
  • Year and Date: 2014-09-02 – 2014-09-04
• [Presentation] The role of kisspeptin for defeminization and masculinization of sexual behaviors in rats (2014)
  • Author(s): Nakamura, S., Uenoyama, Y., Ikegami, K., Tamura, C., Sanbo, M., Hirabayashi, M., Tsukamura, H., Maeda K.-I.
  • Organizer: World Congress of Reproductive Biology 2014
  • Place of Presentation: Edinburgh(Scotland)
  • Year and Date: 2014-09-02 – 2014-09-04
• [Presentation] 低栄養による繁殖抑制を担う脳内エネルギーセンシングメカニズム (2014)
  • Author(s): 美辺 詩織、松本 華代、出浦 慎哉、池上 花奈、後藤 哲平、三宝 誠、平林 真澄、上野山 賀久、前多 敬一郎、束村 博子
  • Organizer: 第107回日本繁殖生物学会大会
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] キスペプチンニューロンを介したGnRH部分ペプチドによるLH分泌促進 (2014)
  • Author(s): 家田 菜穂子美辺 詩織、井上 直子、上野山 賀久、前多 敬一郎、束村 博子
  • Organizer: 第107回日本繁殖生物学会大会
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] キスペプチンノックアウトマウス由来の卵子および精子の受精能の検討 (2014)
  • Author(s): 後藤 哲平、平林 真澄、前多 敬一郎、束村 博子、上野山 賀久
  • Organizer: 第107回日本繁殖生物学会大会
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] 新生児期エストロジェンにより発現が変化する視床下部内遺伝子の網羅的解析 (2014)
  • Author(s): 渡辺 雄貴、榊原 基嗣、上野山 賀久、美辺 詩織、出浦 慎哉、中村 翔、前多 敬一郎、束村 博子
  • Organizer: 第107回日本繁殖生物学会大会
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] NKB-NK3RシグナリングによるKNDyニューロンの神経活動同期メカニズム (2014)
  • Author(s): 池上 花奈、美辺 詩織、家田 菜穂子、安部 仁美、後藤 哲平、前多 敬一郎、束村 博子、上野山 賀久
  • Organizer: 第107回日本繁殖生物学会大会
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] 吸乳刺激によるキスペプチン遺伝子発現抑制を仲介する TIP39-SST 系の 可能性 (2014)
  • Author(s): 杉本 有沙、上野山 賀久、渡辺 雄貴、家田 菜穂子、池上 花奈、井上 直子、束村 博子
  • Organizer: 第107回日本繁殖生物学会大会
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] ラット性行動の発現制御におけるキスペプチンの役割 (2014)
  • Author(s): 中村 翔、上野山 賀久、池上 花奈、田村 千尋、三宝 誠、平林 真澄、束村 博子、前多 敬一郎
  • Organizer: 第107回日本繁殖生物学会大会
  • Place of Presentation: 帯広畜産大学（北海道帯広市）
  • Year and Date: 2014-08-21 – 2014-08-24
• [Presentation] Key role of Kisspeptin-GPR54 signaling in regulating mammalian reproduction: physiological and epigenetic aspects (2014)
  • Author(s): Tsukamura, H.
  • Organizer: International Congress of Neuroendocrinology
  • Place of Presentation: Sydney (Australia)
  • Year and Date: 2014-08-17 – 2014-08-20
  • Invited
• [Presentation] Estrogen-induced changes in gene expressions related to the brain sexual differentiation in rodents (2014)
  • Author(s): Watanabe, Y., Sakakibara, M., Uenoyama, Y., Minabe, S., Deura, C., Nakamura, S., Maeda, K.-I., Tsukamura, H.
  • Organizer: International Congress of Neuroendocrinology 2014
  • Place of Presentation: Sydney (Australia)
  • Year and Date: 2014-08-17 – 2014-08-20
• [Presentation] Cell-to-cell communication via gap junctions may play a role in controlling synchronized activities of KNDy neurons regulating pulsatile secretion of gonadotropin-releasing hormone (2014)
  • Author(s): Ikegami, K., Minabe, S., Ieda, N., Abe, H., Goto, T., Maeda, K.-I., Tsukamura, H., Uenoyama, Y.
  • Organizer: International Congress of Neuroendocrinology 2014
  • Place of Presentation: Sydney (Australia)
  • Year and Date: 2014-08-17 – 2014-08-20
• [Presentation] Perinatal kisspeptin is required for suppression of female sexual behavior in male rats (2014)
  • Author(s): Nakamura, S., Uenoyama, Y., Ikegami, K., Tamura, C., Sanbo, M., Hirabayashi, M., Tsukamura, H., Maeda K.-I.
  • Organizer: International Congress of Neuroendocrinology 2014
  • Place of Presentation: Sydney (Australia)
  • Year and Date: 2014-08-17 – 2014-08-20
• [Presentation] 性腺機能制御に関わる後脳上衣細胞の AMP 活性化プロテインキナーゼ(AMPK) の役割 (2014)
  • Author(s): 美辺詩織、松本華代、出浦慎哉、池上花奈、後藤哲平、三宝誠、 平林真澄、上野山賀久、前多敬一郎、束村博子
  • Organizer: 日本下垂体研究会第 29 回学術集会
  • Place of Presentation: 八王子セミナーハウス（東京都八王子市）
  • Year and Date: 2014-08-08 – 2014-08-10
• [Presentation] GnRH-(1-5)による KNDy ニューロンを介した GnRH/LH 分泌促進機構 (2014)
  • Author(s): 家田菜穂子、美辺詩織、井上直子、上野山賀久、前多敬一郎、束村博子
  • Organizer: 日本下垂体研究会第 29 回学術集会
  • Place of Presentation: 八王子セミナーハウス（東京都八王子市）
  • Year and Date: 2014-08-08 – 2014-08-10
• [Presentation] 「脳の性分化とキスペプチンニューロン」 (2014)
  • Author(s): 束村博子
  • Organizer: 第41回日本毒性学会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県神戸市）
  • Year and Date: 2014-07-02 – 2014-07-04
  • Invited
• [Presentation] Epigenetic Regulation of Kisspeptin Neurons (2014)
  • Author(s): Tsukamura, H.
  • Organizer: 16th International Congress of Endocrinology & the Endocrine Society’s 96th Annual Meeting & Expo
  • Place of Presentation: CHICAGO (USA)
  • Year and Date: 2014-06-21 – 2014-06-24
  • Invited
• [Presentation] NKB-NK3RシグナリングによるKNDyニューロンの神経活動制御メカニズム (2014)
  • Author(s): 池上花奈、美辺詩織、家田菜穂子、安部仁美、後藤哲平、前多敬一郎、束村博子、上野山賀久
  • Organizer: 第11回GPCR研究会
  • Place of Presentation: 日本科学未来館（東京都江東区）
  • Year and Date: 2014-05-09 – 2014-05-10
• [Book] Fetal/perinatal programming causing sexual dimorphism of the kisspeptin-GnRH neuronal network. In: Herbison AE, Plant TM eds The GnRH Neuron and its Control, (2018)
  • Author(s): Tsukamura H, Maeda KI, Uenoyama Y, 43-60.
  • Total Pages: 517
  • Publisher: Wiley