| Budget Amount *help |
¥38,610,000 (Direct Cost: ¥29,700,000、Indirect Cost: ¥8,910,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2014: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
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| Outline of Final Research Achievements |
The present study aimed to investigate the mechanism mediating estrogen negative and positive feedback regulating follicular development and ovulation, respectively, in mammals. The study focused on the effects of estrogen on Kiss1 (kisspeptin gene) expression in kisspeptin neurons, and demonstrates that the arcuate (ARC) and anteroventral paraventricular nucleus (AVPV)/ preoptic kisspeptin neurons are targets of estrogen to exert the estrogen negative and positive feedback effects, respectively, in rats, mouse, monkeys, and goats. The study also revealed that specific transcriptional factors are expressed in the both kisspeptin neuronal populations. Further, the present study tried to generate immortalized rat AVPV and ARC kisspeptin neuronal cell lines to investigate molecular mechanism mediating the estrogen effects. As a result, we obtained some kisspeptin neuronal cell line candidates from the AVPV and ARC.
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