| Budget Amount *help |
¥39,910,000 (Direct Cost: ¥30,700,000、Indirect Cost: ¥9,210,000)
Fiscal Year 2017: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2016: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2015: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Outline of Final Research Achievements |
Lepidopteran insects are known to have specific chromosomes called heliocentric chromosome having multiple centromeres along the entire length. In this study, we clarified the molecular basis of kinetochore formation of the heliocentric chromosome and newly found six centromere-related genes specific to silkworm using the gene network analysis. Based on these results and analysis on replication related genes, an artificial chromosome functioning by tethering DNA replication factor and kinetochore protein was synthesized.
On the other hand, as for the genome-wide gene expression regulation and molecular basis of chromatin structure, we focused on histone acetylation and deacetylation enzymes, and performed gene knockdown on these genes. As a result, we found that HDAC 8 plays an important role in the silkworm.
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