Mechanisms of symbiotic evolution and N2O mitigation of soybean bradyrhizobia

• Project/Area Number: 26252065
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Tohoku University
• Principal Investigator: Minamisawa Kiwamu 東北大学, 生命科学研究科, 教授 (70167667)
• Co-Investigator(Kenkyū-buntansha): 佐藤 修正 東北大学, 生命科学研究科, 准教授 (70370921) ; 内田 隆史 東北大学, (連合)農学研究科(研究院), 教授 (80312239)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥40,300,000 (Direct Cost: ¥31,000,000、Indirect Cost: ¥9,300,000); Fiscal Year 2016: ¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000); Fiscal Year 2015: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000); Fiscal Year 2014: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
• Keywords: 生物間相互作用 / 共生 / 脱窒 / タンパク質分泌系 / エフェクター / 根粒菌 / ダイズ / 病原システム / 温室効果ガス / アンチターミネーション

Outline of Final Research Achievements

Rj2 restricts nodulation by specific rhizobial strains including Bradyrhizobium diazoefficiens USDA122. We identified a T3SS effector gene that induces the incompatibility to Rj2-soybeans. These results suggested that hosts monitor the rhizobial T3SS effector during infection and reject nodulation via host R protein. B. diazoefficiens is able to scavenge the greenhouse gas N2O through the N2O reductase. Comparative analysis of Nos(++) mutant genomes showed that nasS mutation of resulted in Nos(++) phenotype. nasS mutation induced nosZ via NasT. NO3(-) addition dissociated the NasS-NasT complex in vitro, suggesting the release of the activator NasT. Disruption of nasT led to a marked decrease in nosZ transcription. Thus NasST regulates the NO3(-) -mediated response of nosZ gene. EMSA analysis suggested that nos expression is subjected to antitermination on both H1 and H2 hairpins upstream of nosR.

Research Products

• [Journal Article] Complete genome sequence of Bradyrhizobium japonicum J5, isolated from a soybean nodule in Hokkaido, Japan (2017)
  • Author(s): Kazuma Kanehara, Kiwamu Minamisawa.
  • Journal Title: Genome Announc. Volume: 5(6) Issue: 6
  • DOI: 10.1128/genomea.01619-16
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Complete genome sequence of Bradyrhizobium diazoefficiens USDA 122,a nitrogen-fixing soybean symbiont (2017)
  • Author(s): Masayuki Sugawara, Takahiro Tsukui, Takakazu Kaneko, Yoshiyuki Ohtsubo, Shusei Sato, Yuji Nagata, Masataka Tsuda, Hisayuki Mitsui, Kiwamu Minamisawa.
  • Journal Title: Genome Announc. Volume: 5(9) Issue: 9
  • DOI: 10.1128/genomea.01743-16
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Origin and Evolution of Nitrogen Fixation Genes on Symbiosis Islands and Plasmid in <i>Bradyrhizobium</i> (2016)
  • Author(s): Takashi Okubo, Pongdet Piromyou, Panlada Tittabutr, Neung Teaumroong, and Kiwamu Minamisawa.
  • Journal Title: Microbes and Environments Volume: 31 Issue: 3 Pages: 260-267
  • DOI: 10.1264/jsme2.ME15159
  • NAID: 130005265078
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Metagenomic Analysis Revealed Methylamine and Ureide Utilization of Soybean-Associated <i>Methylobacterium</i> (2016)
  • Author(s): Tomoyuki Minami, Misue Anda, Hisayuki Mitsui, Masayuki Sugawara, Takakazu Kaneko, Shusei Sato, Seishi Ikeda, Takashi Okubo, Hirohito Tsurumaru, Kiwamu Minamisawa.
  • Journal Title: Microbes and Environments Volume: 31 Issue: 3 Pages: 268-278
  • DOI: 10.1264/jsme2.ME16035
  • NAID: 130005265032
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Draft genome sequence of Methylosinus sp. strain 3S-1, an isolate from rice root in a low-N paddy field. (2016)
  • Author(s): Zhihua Bao, Ryo Shinoda, and Kiwamu Minamisawa.
  • Journal Title: Genome Announc. Volume: 4(5) Issue: 5
  • DOI: 10.1128/genomea.00932-16
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mitigation of soil N2O emission by inoculation with a mixed culture of indigenous Bradyrhizobium diazoefficiens. (2016)
  • Author(s): Hiroko Akiyama, Yuko Takada Hoshino, Manabu Itakura, Yumi Shimomura, Yong Wang, Akinori Yamamoto, Kanako Tago, Yasuhiro Nakajima, Kiwamu Minamisawa & Masahito Hayatsu.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 32869-32869
  • DOI: 10.1038/srep32869
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] sNOOOpy, a Sensor for Physiological NO3－/NO2－ Employing a Bacterial Two-Component Regulatory System. (2016)
  • Author(s): Hidaka M, Gotoh A, Shimizu T, Minamisawa K, Imamura H and Uchida T
  • Journal Title: Journal Biological Chemistry Volume: 291 Issue: 5 Pages: 2260-2269
  • DOI: 10.1074/jbc.m115.687632
  • Peer Reviewed
• [Journal Article] Identification of the Hydrogen Uptake Gene Cluster for Chemolithoautotrophic Growth and Symbiosis Hydrogen Uptake in <i>Bradyrhizobium Diazoefficiens</i> (2016)
  • Author(s): Sachiko Masuda, Masaki Saito, Chiaki Sugawara, Manabu Itakura, Shima Eda, Kiwamu Minamisawa
  • Journal Title: Microbes and Environments Volume: 31 Issue: 1 Pages: 76-78
  • DOI: 10.1264/jsme2.ME15182
  • NAID: 130005138801
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sulfur Fertilization Changes the Community Structure of Rice Root-, and Soil- Associated Bacteria (2016)
  • Author(s): Sachiko Masuda, Zhihua Bao, Takashi Okubo, Kazuhiro Sasaki, Seishi Ikeda, Ryo Shinoda, Mizue Anda, Ryuji Kondo, Yumi Mori, Kiwamu Minamisawa
  • Journal Title: Microbes and Environments Volume: 31 Issue: 1 Pages: 70-75
  • DOI: 10.1264/jsme2.ME15170
  • NAID: 130005138796
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Are Symbiotic Methanotrophs Key Microbes for N Acquisition in Paddy Rice Root? (2016)
  • Author(s): Kiwamu Minamisawa, Haruko Imaizumi-Anraku, Zhihua Bao, Ryo Shinoda, Takashi Okubo, Seishi Ikeda
  • Journal Title: Microbes and Environments Volume: 31 Issue: 1 Pages: 4-10
  • DOI: 10.1264/jsme2.ME15180
  • NAID: 130005138800
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Complete genome sequence of Methylobacterium sp. strain AMS5, an isolate from a soybean stem (2016)
  • Author(s): Tomoyuki Minami, Yoshiyuki Ohtsubo, Mizue Anda, Yuji Nagata, Masataka Tsuda, Hisayuki Mitsui, Masayuki Sugawara, Kiwamu Minamisawa
  • Journal Title: Genome Announc. Volume: 4(2) Issue: 2
  • DOI: 10.1128/genomea.00144-16
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Preference of endophytic bradyrhizobia in different rice cultivars and the implication of rice endophyte evolution (2015)
  • Author(s): Piromyou, P., T. Greetatorn, K. Teamtisong, T. Okubo, R. Shinoda, A. Nuntakij, P. Tittabutr, N. Boonkerd, K. Minamisawa, and N. Teaumroong
  • Journal Title: Appl. Environ. Microbiol. Volume: in press Issue: 9 Pages: 3049-3061
  • DOI: 10.1128/aem.04253-14
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Symbiosis island shuffling with abundant insertion sequences in the genomes of extra-slow-growing strains of soybean bradyrhizobia (2015)
  • Author(s): Takayuki Iida, Manabu Itakura, Mizue Anda, Masayuki Sugawara, Tsuyoshi Isawa, Takashi Okubo, Shusei Sato, Kaori Chiba-Kakizaki and Kiwamu Minamisawa
  • Journal Title: Appl. Environ. Microbiol. Volume: 81(12) Issue: 12 Pages: 4143-4154
  • DOI: 10.1128/aem.00741-15
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Bacterial clade with the ribosomal RNA operon on a small plasmid rather than the chromosome (2015)
  • Author(s): Mizue Anda, Yoshiyuki Ohtsubo, Takashi Okubo, Masayuki Sugawara, Yuji Nagata, Masataka Tsuda, Kiwamu Minamisawa, and Hisayuki Mitsui
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 112(46) Issue: 46 Pages: 14343-14347
  • DOI: 10.1073/pnas.1514326112
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The type III secretion system (T3SS) is a determinant for rice-endophyte colonization by non-photosynthetic Bradyrhizobium (2015)
  • Author(s): Pongdet Piromyou, Pongpan Songwattana, Teerana Greetatorn, Takashi Okubo, Kaori Chiba Kakizaki, Janpen Prakamhang, Panlada Tittabutr, Nantakorn Boonkerd, Neung Teaumroong, Kiwamu Minamisawa
  • Journal Title: Microbes Environ. Volume: 30(4) Issue: 4 Pages: 291-300
  • DOI: 10.1264/jsme2.me15080
  • NAID: 130005116036
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Elevated atmospheric CO2 levels affect community structure of rice root-associated bacteria (2015)
  • Author(s): Takashi Okubo, Dongyan Liu, Hirohito Tsurumaru, Seishi Ikeda, Susumu Asakawa, Takeshi Tokida, Kanako Tago, Masahito Hayatsu, Naohiro Aoki, Ken Ishimaru, Kazuhiro Ujiie, Yasuhiro Usui, Hirofumi Nakamura, Hidemitsu Sakai, Kentaro Hayashi, Toshihiro Hasegawa and Kiwamu Minamisawa
  • Journal Title: Frontiers in Microbiology Volume: 6 Pages: 1-8
  • DOI: 10.3389/fmicb.2015.00136
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Metagenomic Analysis of the Bacterial Community Associated with the Taproot of Sugar Beet (2015)
  • Author(s): Hirohito Tsurumaru, Takashi Okubo, Kazuyuki Okazaki, Megumi Hashimoto, Kaori Kakizaki, Eiko Hanzawa, Hiroyuki Takahashi, Noriyuki Asanome, Fukuyo Tanaka, Yasuyo Sekiyama, Seishi Ikeda, and Kimwamu Minamisawa
  • Journal Title: Microbes and Environments Volume: 30 Issue: 1 Pages: 63-69
  • DOI: 10.1264/jsme2.ME14109
  • NAID: 130005061718
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characterization of Leaf Blade- and Leaf Sheath-Associated Bacterial Communities and Assessment of Their Responses to Environmental Changes in CO₂, Temperature, and Nitrogen Levels under Field Conditions (2015)
  • Author(s): Seishi Ikeda, Takeshi Tokida, Hirofumi Nakamura, Hidemitsu Sakai, Yasuhiro Usui, Takashi Okubo, Kanako Tago, Kentaro Hayashi, Yasuyo Sekiyama, Hiroshi Ono, Satoru Tomita, Masahito Hayatsu, Toshihiro Hasegawa, and Kiwamu Minamisawa
  • Journal Title: Microbes and Environments Volume: 30 Issue: 1 Pages: 51-62
  • DOI: 10.1264/jsme2.ME14117
  • NAID: 130005061713
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Relationship Between Soil Type and N₂O Reductase Genotype (<i>nosZ</i>) of Indigenous Soybean Bradyrhizobia: <i>nosZ</i>-minus Populations are Dominant in Andosols (2014)
  • Author(s): Shiina, Y., M. Itakura, H. Choi, Y. Saeki, M. Hayatsu, and K. Minamisawa
  • Journal Title: Microbes and Environments Volume: 29 Issue: 4 Pages: 420-426
  • DOI: 10.1264/jsme2.ME14130
  • NAID: 130004703875
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Phylogeny and Functions of Bacterial Communities Associated with Field-Grown Rice Shoots (2014)
  • Author(s): Takashi Okubo, Seishi Ikeda, Kazuhiro Sasaki, Kenshiro Ohshima, Masahira Hattori, Tadashi Sato, and
  • Journal Title: Microbes and Environments Volume: 29 Issue: 3 Pages: 329-332
  • DOI: 10.1264/jsme2.ME14077
  • NAID: 130004677526
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The nitrate-sensing NasST system regulates nitrous oxide reductase and periplasmic nitrate reductase in Bradyrhizobium japonicum. (2014)
  • Author(s): Sanchez, C., M. Itakura, T. Okubo, T. Matsumoto, H. Yoshikawa, A. Gotoh, M. Hidaka, T. Uchida, and K. Minamisawa.
  • Journal Title: Environmental Microbiology Volume: 16 Issue: 10 Pages: 3263-3274
  • DOI: 10.1111/1462-2920.12546
  • Peer Reviewed / Open Access
• [Journal Article] Metaproteomic Identification of Diazotrophic Methanotrophs, and their Tissue Localization in Field-grown Rice Roots (2014)
  • Author(s): Bao, Z., T. Okubo, K. Kubota, Y. Kasahara, H. Tsurumaru, M. Anda, S. Ikeda, K. Minamisawa
  • Journal Title: Applied and Environmental Microbiology Volume: 80 Issue: 16 Pages: 5043-5052
  • DOI: 10.1128/aem.00969-14
  • Peer Reviewed
• [Journal Article] NODULE INCEPTION creates a long-distance negative feedback loop involved in homeostatic regulation of nodule organ production. (2014)
  • Author(s): Soyano, T., Hirakawa, H., Sato, S., *Hayashi, M., and Kawaguchi, M.
  • Journal Title: Proc Natl Acad Sci USA Volume: 111 Issue: 40 Pages: 14607-14612
  • DOI: 10.1073/pnas.1412716111
  • Peer Reviewed
• [Presentation] ダイズとBradyrhizobium diazoefficiensとの共生不和合性を誘導する根粒菌3型分泌エフェクター (2017)
  • Author(s): 菅原雅之 ，岩野裕也 ，今道仁 ，高橋智子 ，佐藤修正 ，梅原洋佐 ，三井 久幸 ，南澤究
  • Organizer: 日本農芸化学会2017年度大会
  • Place of Presentation: ウェスティン都ホテル京都、京都女子大学（京都府京都市）
  • Year and Date: 2017-03-17
• [Presentation] 開花特性からみたミヤコグサの地域適応戦略の解析 (2017)
  • Author(s): 川村弥司子,王明卓,日下部翔平,菊池裕喜,佐藤修正
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学 群元キャンパス（鹿児島県鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] ダイズ根粒菌3型分泌エフェクター遺伝子の多様性 (2017)
  • Author(s): 高橋智子、菅原雅之、三井久幸、南澤究
  • Organizer: 第11回日本ゲノム微生物学会年会
  • Place of Presentation: 慶應義塾大学 湘南藤沢キャンパス（神奈川県藤沢市）
  • Year and Date: 2017-03-02
• [Presentation] 温室効果ガスを減らす植物共生細菌 (2017)
  • Author(s): 南澤究
  • Organizer: 日本生物工学会東日本支部賀詞交歓会
  • Place of Presentation: アサヒビールタワー（東京都千代田区）
  • Invited
• [Presentation] Frontiers of plant-associated bacteria (2016)
  • Author(s): Kiwamu Minamisawa
  • Organizer: 4th Asian Conference Plant-Microbe Symbiosis and Nitrogen Fixation (4APMNF)
  • Place of Presentation: PARKROYAL Penang Resort（Penang, Malaysia）
  • Year and Date: 2016-10-16
  • Int'l Joint Research / Invited
• [Presentation] Differences in the denitrification pathway between Bradyrhizobium diazoefficiens and B. japonicum under strictly anaerobic conditions (2016)
  • Author(s): Arthur Fernandes Siqueira, Cristina Sanchez, Kiwamu Minamisawa
  • Organizer: 4th Asian Conference Plant-Microbe Symbiosis and Nitrogen Fixation (4APMNF)
  • Place of Presentation: PARKROYAL Penang Resort（Penang, Malaysia）
  • Year and Date: 2016-10-16
  • Int'l Joint Research
• [Presentation] Rhizobial effector protein inducing the symbiotic incompatibility between Bradyrhizobium diazoefficiens and soybean plants (2016)
  • Author(s): Masayuki Sugawara, Hiroya Iwano, Hitoshi Kondo, Satoko Takahashi, Shohei Kusakabe, Shusei Sato, Yosuke Umehara, Hisayuki Mitsui, Kiwamu Minamisawa
  • Organizer: 4th Asian Conference Plant-Microbe Symbiosis and Nitrogen Fixation (4APMNF)
  • Place of Presentation: PARKROYAL Penang Resort（Penang, Malaysia）
  • Year and Date: 2016-10-16
  • Int'l Joint Research
• [Presentation] ダイズとBradyrhizobium diazoefficiensとの共生不和合性を誘導する根粒菌エフェクター (2016)
  • Author(s): 菅原雅之 ，岩野裕也 ，今道仁 ，高橋智子 ，日下部翔平 ，佐藤修正 ，梅原洋佐 ，三井 久幸 ，南澤究
  • Organizer: 植物微生物研究会第26回研究交流会
  • Place of Presentation: 東北大学片平キャンパスさくらホール（宮城県仙台市）
  • Year and Date: 2016-09-07
• [Presentation] 共生不和合性を誘導する根粒菌エフェクターのダイズによる認識と応答 (2016)
  • Author(s): 今道仁, 菅原雅之, 岩野裕也, 佐藤修正, 三井久幸, 南澤究
  • Organizer: 植物微生物研究会第26回研究交流会
  • Place of Presentation: 東北大学片平キャンパスさくらホール（宮城県仙台市）
  • Year and Date: 2016-09-07
• [Presentation] ダイズ根粒菌３型分泌エフェクター遺伝子の多様性 (2016)
  • Author(s): 高橋智子、鈴木悠太、菅原雅之、三井久幸、南澤究
  • Organizer: 植物微生物研究会第26回研究交流会
  • Place of Presentation: 東北大学片平キャンパスさくらホール（宮城県仙台市）
  • Year and Date: 2016-09-07
• [Presentation] Enrichment of nasS mutant of Bradyrhizobium diazoefficiens with higher N2O reductase activity to recover naturally-rare nosZ++ strains (2016)
  • Author(s): Arthur F. Siqueira, Manabu Itakura, Kiwamu Minamisawa
  • Organizer: 植物微生物研究会第26回研究交流会
  • Place of Presentation: 東北大学片平キャンパスさくらホール（宮城県仙台市）
  • Year and Date: 2016-09-07
• [Presentation] Functional characterization of Bradyrhizobium oligotrophicum S58 Cu-type and cd1-type nitrite reductase genes (2016)
  • Author(s): Cristina Sanchez, Kiwamu Minamisawa
  • Organizer: 植物微生物研究会第26回研究交流会
  • Place of Presentation: 東北大学片平キャンパスさくらホール（宮城県仙台市）
  • Year and Date: 2016-09-07
• [Presentation] Plant-associated Aureimonas with the sole rRNA operon on a tiny plasmid (2016)
  • Author(s): Kiwamu Minamisawa
  • Organizer: 16th International Symposium on Microbial Ecology
  • Place of Presentation: Palais des Congres de Montreal（Montreal, Canada）
  • Year and Date: 2016-08-21
  • Int'l Joint Research / Invited
• [Presentation] メタゲノム解析によるダイズ Methylobacterium 属細菌のメチルアミン・ウレイド代謝の発見 (2016)
  • Author(s): 南智之・按田瑞恵・三井久幸・菅原雅之・金子貴一・佐藤修正・池田成志・南澤究
  • Organizer: 日本土壌微生物学会2016年度大会
  • Place of Presentation: 岐阜大学全学共通教育講義棟（岐阜県岐阜市）
  • Year and Date: 2016-06-11
• [Presentation] Differences in the denitrification pathway between Bradyrhizobium diazoefficiens and Bradyrhizobium japonicum under strictly anaerobic conditions (2016)
  • Author(s): Arthur F. Siqueira・Cristina Sanchez・Manabu Itakura・Kiwamu Minamisawa
  • Organizer: 日本土壌微生物学会2016年度大会
  • Place of Presentation: 岐阜大学全学共通教育講義棟（岐阜県岐阜市）
  • Year and Date: 2016-06-11
• [Presentation] 植物共生微生物のエコゲノミクスによる物質循環能の解明 (2016)
  • Author(s): 南澤 究
  • Organizer: 拠点共同研究ワークショップ「微小生態系の構成原理~Wet/Dry双方からのアプローチによる理解と制御の試み」
  • Place of Presentation: 岡山大学資源植物科学研究所 (岡山県倉敷市)
  • Year and Date: 2016-02-15
  • Invited
• [Presentation] 植物共生微生物の役割と農業 (2016)
  • Author(s): 南澤 究
  • Organizer: 2016年 マルタ全国大会
  • Place of Presentation: TKPガーデンシティ品川 (東京都港区)
  • Year and Date: 2016-02-12
  • Invited
• [Presentation] 食料生産・環境分野における微生物ゲノミクスの可能性と課題 (2015)
  • Author(s): 南澤 究
  • Organizer: 生命医薬情報学連合大会 2015 年大会 （日本バイオインフォマティクス学会 2015 年年会）
  • Place of Presentation: 京都大学宇治キャンパスおうばくプラザ (京都府宇治市)
  • Year and Date: 2015-10-29
  • Invited
• [Presentation] Rhizosphere is a textbook for microbial N cycle (2015)
  • Author(s): Kiwamu Minamisawa
  • Organizer: 日本微生物生態学会第30回大会 2015 JSME annual meeting & 7th JTK symposium
  • Place of Presentation: 土浦市役所 亀城プラザ (茨城県土浦市)
  • Year and Date: 2015-10-17
  • Int'l Joint Research / Invited
• [Presentation] N2O emission from soybean rhizosphere and its mitigation by Bradyrhizobium diazoefficiens inoculation (2015)
  • Author(s): Manabu Itakura, Kiwamu Minamisawa
  • Organizer: 日本微生物生態学会第30回大会 2015 JSME annual meeting & 7th JTK symposium
  • Place of Presentation: 土浦市役所 亀城プラザ (茨城県土浦市)
  • Year and Date: 2015-10-17
  • Int'l Joint Research / Invited
• [Presentation] Regulation of N2O reductase genes by the two-component system NasST in Bradyrhizobium diazoefficiens (2015)
  • Author(s): Cristina Sanchez, Kiwamu Minamisawa
  • Organizer: 日本微生物生態学会第30回大会 2015 JSME annual meeting & 7th JTK symposium
  • Place of Presentation: 土浦市役所 亀城プラザ (茨城県土浦市)
  • Year and Date: 2015-10-17
  • Int'l Joint Research / Invited
• [Presentation] "Differential efficiency of nitrate respiration between Bradyrhizbium diazoefficiens and Bradyrhizobium japonicum" (2015)
  • Author(s): Arthur F. Siqueira, Cristina Sanchez, Manabu Itakura, Kiwamu Minamisawa
  • Organizer: 日本微生物生態学会第30回大会 2015 JSME annual meeting & 7th JTK symposium
  • Place of Presentation: 土浦市役所 亀城プラザ (茨城県土浦市)
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] N2O reductase gene and activity in bradyrhizobia nodulating Aeschynomene indica (2015)
  • Author(s): Tomohiro Kumei, Masayuki Sugawara, Cristina Sanchez, Kiwamu Minamisawa
  • Organizer: 日本微生物生態学会第30回大会 2015 JSME annual meeting & 7th JTK symposium
  • Place of Presentation: 土浦市役所 亀城プラザ (茨城県土浦市)
  • Year and Date: 2015-10-17
• [Presentation] Regulation of nitrous oxide reductase genes by the NasST system in Bradyrhizobium diazoefficiens (2015)
  • Author(s): Cristina Sanchez, Manabu Itakura, Takashi Okubo, Takashi Matsumoto, Hirofumi Yoshikawa, Aina Gotoh, Masafumi Hidaka, Takafumi Uchida, Kiwamu Minamisawa
  • Organizer: 20th European Nitrogen Cycle Meeting
  • Place of Presentation: The University of Aberdeen (UK)
  • Year and Date: 2015-09-28
  • Int'l Joint Research
• [Presentation] 日本のダイズ根粒菌とダイズのRj2共生不和合性 (2015)
  • Author(s): 菅原雅之・鈴木悠太・板倉学・千葉(柿崎)芳里・佐藤修正・加賀秋人・石本政男・南 澤 究
  • Organizer: 日本土壌微生物学会2015年度大会
  • Place of Presentation: つくば国際会議場 (茨城県つくば市)
  • Year and Date: 2015-05-22
• [Presentation] ワークショップ「入れ子型構造か彩る新生命観」根粒菌共生アイラントのマトリョーシカ型進化 (2015)
  • Author(s): 南澤究
  • Organizer: 第89回細菌学会総会
  • Place of Presentation: 長良川国際会議場（岐阜市）
  • Year and Date: 2015-03-23 – 2015-03-25
  • Invited
• [Presentation] 日本ダイズコアコレクションにおける共生不和合性Rj2遺伝型の分布 (2015)
  • Author(s): 菅原雅之、南澤究
  • Organizer: 第８回ダイズ研究会
  • Place of Presentation: 岩手大学農学部（岩手県盛岡市）
  • Year and Date: 2015-03-06 – 2015-03-07
• [Presentation] Establishment of the Lotus japonicus genomic information toward functional genomics. (2014)
  • Author(s): Shusei Sato
  • Organizer: 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: The New International Convention & Exposition Center (Chengdu, China)
  • Year and Date: 2014-10-28 – 2014-10-30
  • Invited
• [Presentation] 共生不和合性を誘導するダイズ根粒菌のタイプIII型エフェクターの探索 (2014)
  • Author(s): 岩野裕也、鈴木悠太、柿崎芳里、菅原雅之、佐藤修正、南澤究
  • Organizer: 環境微生物系学会合同大会2014
  • Place of Presentation: 浜松アクトシティ コングレスセンター（静岡県浜松市)
  • Year and Date: 2014-10-21 – 2014-10-24
• [Presentation] 微生物ゲノム情報を圃場で活かす ― 作物根圏からの温室効果ガス発生を制御するために ― (2014)
  • Author(s): 南澤究
  • Organizer: 平成２６年度日本農学会シンポジウム
  • Place of Presentation: 東京大学弥生講堂（東京都文京区）
  • Year and Date: 2014-10-04
  • Invited
• [Presentation] 共生窒素固定を促進するダイズ根粒菌III型分泌系エフェクター (2014)
  • Author(s): 鈴木悠太、Cristina Sanchez、柿崎芳里、菅原雅之、佐藤修正、金子貴一、南澤究
  • Organizer: 第24回植物微生物研究交流会
  • Place of Presentation: 佐賀大学農学部（佐賀市）
  • Year and Date: 2014-09-19 – 2014-09-21
• [Presentation] 共生窒素固定を促進するダイズ根粒菌III型分泌系エフェクター (2014)
  • Author(s): 鈴木悠太、Cristina Sanchez、柿崎芳里、佐藤修正、金子貴一、南澤究
  • Organizer: 日本土壌肥料学会2014年度東京大会
  • Place of Presentation: 東京農工大学小金井キャンパス（東京都小金井市）
  • Year and Date: 2014-09-09 – 2014-09-11
• [Presentation] The nitrate-sensing NasST system regulates nitrous oxide reductase in Bradyrhizobium japonicum (2014)
  • Author(s): Cristina Sanchez, Manabu Itakura, Takashi Okubo, Takashi Matsumoto, Hirofumi Yoshikawa, Aina Gotoh, Masafumi Hidaka, Takafumi Uchida, Kiwamu Minamisawa
  • Organizer: 15th International Symposium on Microbial Ecology
  • Place of Presentation: Coex Comvention Center (Seoul, Korea)
  • Year and Date: 2014-08-24 – 2014-08-29
• [Book] 共生微生物 生物と密接に関わるミクロな生命体 (2016)
  • Author(s): 南澤究
  • Total Pages: 281
  • Publisher: 化学同人
• [Book] 肥料科学 (2016)
  • Author(s): 南澤究
  • Publisher: 肥料科学研究所
• [Book] シリーズ21世紀の農学 ここまで進んだ！飛躍する農学 (2015)
  • Author(s): 南澤 究
  • Total Pages: 171
  • Publisher: 養賢堂 日本農学会編
• [Book] シリーズ21世紀の農学 ここまで進んだ！飛躍する農学 (2015)
  • Author(s): 南澤究
  • Total Pages: 171
  • Publisher: 株式会社 養賢堂 編集者：日本農学会
• [Book] The Lotus japonicus genome (Chapter 4. Genome sequencing) (2014)
  • Author(s): Shusei Sato and Stig Andersen
  • Total Pages: 267
  • Publisher: Springer