| Budget Amount *help |
¥40,560,000 (Direct Cost: ¥31,200,000、Indirect Cost: ¥9,360,000)
Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2017: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2015: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2014: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Outline of Final Research Achievements |
In this study, we dissected the molecular mechanism in the formation of esophago-gastric (EC) junction during organogenesis. We found that Sox2 and Gata4 are initially co-expressed in the progenitor epithelia then expressions of Sox2 and Gata4 gradually formed gradients in inverse direction across the EC junction. Meanwhile, Fgf2 was induced in the gastric mesenchyme and proximal epithelial cells expressed FgfR2, resulting in the restricted activation of ERK at the junction. Separately sorted Gata4-low, -mid and -high epithelial cells at E13.5 formed stratified squamous, pseudostratified and simple columnar organoid, respectively, by in vitro culture. Furthermore, Fgf2 treatment to Gata4-low cells inhibited the differentiation to stratified squamous epithelia and stimulated the differentiation to transitional epithelia correspond to the E-C junction. Thus, spatio-temporal patterning of epithelial and mesenchymal Sox2/Gata4/ Fgf2 is important for the construction of EC junction.
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