Regulation and determination of organ derived from invaginated epithelium
Project/Area Number |
26253092
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Orthodontics/Pediatric dentistry
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Research Institution | Tohoku University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
保住 建太郎 東京薬科大学, 薬学部, 講師 (10453804)
江草 宏 東北大学, 歯学研究科(研究院), 教授 (30379078)
齋藤 正寛 東北大学, 歯学研究科(研究院), 教授 (40215562)
齋藤 幹 東北大学, 大学病院, 講師 (40380852)
阪井 丘芳 大阪大学, 歯学研究科(研究院), 教授 (90379082)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥41,730,000 (Direct Cost: ¥32,100,000、Indirect Cost: ¥9,630,000)
Fiscal Year 2016: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2015: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2014: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
|
Keywords | 上皮陥入 / 器官形成 / 歯の再生 / 発生医学 / 再生 / エナメル質 / 毛 / 歯の発生 / 歯学 / 再生医学 / シグナル伝達 / 細胞・組織 / 生体分子 |
Outline of Final Research Achievements |
Tooth and hair are formed by invagination of epithelium. Invaginated epithelium interacts with neural crest derived mesenchymal tissues, and then formed tooth and hair. However, mechanism of organ determination has never clearly understood. Sox21 is one of the transcription factor, and expressed in dental epithelium. Sox21 knockout mice showed hair formation in incisor indicating that Sox21 may be involved in the determination of organs. Decrease Sox21 inhibited ameloblast marker gene expression, and induced hair marker genes including keratins. ChipSeq analysis showed that Sox21 directly binds to Anapc10 gene promoter region and regulated their expression in dental epithelium. In Sox21 knockout mice, Anapc10 expression was decreased in tooth germ. Further, Sox21 over expression induced Anapc10 expression. These results suggested that Sox21 regulates Anapc10 expression and organ determination.
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Nephronectin plays critical roles in Sox2 expression and proliferation in dental epithelial stem cells via EGF-like repeat domains.2017
Author(s)
Arai C, Yoshizaki K, Miyazaki K, Saito K, Yamada A, Han X, Funada K, Fukumoto E, Haruyama N, Iwamoto T, Takahashi I, Fukumoto S.
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Journal Title
Sci Rep.
Volume: 27
Issue: 1
Pages: 45181-45181
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Practical whole-tooth restoration utilizing autologous bioengineered tooth germ transplantation in a postnatal canine model.2017
Author(s)
Ono M, Oshima M, Ogawa M, Sonoyama W, Hara ES, Oida Y, Shinkawa S, Nakajima R , Mine A ,Hayano S, Fukumoto S , Kasugai8 S , Yamaguchi A, Tsuji T, Kuboki T
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Journal Title
Sci Rep
Volume: 7
Issue: 1
Pages: 44522-44522
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] The SCFβ-TRCP E3 ubiquitin ligase complex targets Lipin1 for ubiquitination and degradation to promote hepatic lipogenesis.2017
Author(s)
Shimizu K, Fukushima H, Ogura K, Lien EC, Nihira NT, Zhang J, North BJ, Guo A, Nagashima K, Nakagawa T, Hoshikawa S, Watahiki A, Okabe K, Yamada A, Toker A, Asara JM, Fukumoto S, Nakayama KI, Nakayama K, Inuzuka H, Wei W.
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Journal Title
Sci Signal. doi: 10.1126/scisignal.aah4117.
Volume: 10
Issue: 460
Pages: 1-15
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Nutrient-induced FNIP degradation by SCFβ-TRCP regulates FLCN couplex localization and promotes renal cancer progression2016
Author(s)
Nagashima, K., Fukushima, H., Shimizu, K., Hidaka, M., Hasumi, H., Ikebe, T., Fukumoto, S., Okabe, K., Inuzuka, H.
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Journal Title
Oncotarget
Volume: 8
Issue: 6
Pages: 9947-9960
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1/5/8 phosphorylation2016
Author(s)
Liu J, Saito K*, Maruya Y, Nakamura T, Yamada A, Fukumoto E, Ishikawa M, Iwamoto T, Miyazaki K, Yoshizaki K, Ge L, Fukumoto S
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Journal Title
Scientific reports
Volume: 6
Issue: 1
Pages: 23670-23670
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Plakophilin-1, a novel Wnt signaling regulator, is critical for tooth development and ameloblast differentiation2016
Author(s)
Miyazaki K, Yoshizaki K, Arai C, Yamada A, Saito K, Ishikawa M, Xue H, Funada K, Haruyama N, Yamada Y, Fukumoto S, Takahashi I
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Journal Title
Plos One
Volume: 11
Issue: 3
Pages: 0152206-0152206
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Connexin 43 is necessary for salivary gland branching morphogenesis and FGF10-induced ERK1/2 phosphorylation2016
Author(s)
Yamada A, Futagi M, Fukumoto E, Saito K, Yoshizaki K, Ishikawa M, Arakaki M, Hino R, Sugawara Y, Ishikawa M, Naruse M, Miyazaki K, Nakamura T, Fukumoto S.
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Journal Title
The Journal of Biological Chemistry
Volume: 291
Issue: 2
Pages: 904-912
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Interaction between Fibronectin and β1 Integrin is Essential for Tooth Development.2015
Author(s)
Saito, K., Fukumoto, E., Yamada, A., Yuasa, K., Yoshizaki, K., Iwamoto, T., Saito, M., Nakamura, T., and Fukumoto, S.
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Journal Title
PLOS one
Volume: 10(4)
Issue: 4
Pages: 1-12
DOI
NAID
Related Report
Peer Reviewed / Open Access
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