| Project/Area Number |
26253092
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
保住 建太郎 東京薬科大学, 薬学部, 講師 (10453804)
江草 宏 東北大学, 歯学研究科(研究院), 教授 (30379078)
齋藤 正寛 東北大学, 歯学研究科(研究院), 教授 (40215562)
齋藤 幹 東北大学, 大学病院, 講師 (40380852)
阪井 丘芳 大阪大学, 歯学研究科(研究院), 教授 (90379082)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥41,730,000 (Direct Cost: ¥32,100,000、Indirect Cost: ¥9,630,000)
Fiscal Year 2016: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2015: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2014: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
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| Keywords | 上皮陥入 / 器官形成 / 歯の再生 / 発生医学 / 再生 / エナメル質 / 毛 / 歯の発生 / 歯学 / 再生医学 / シグナル伝達 / 細胞・組織 / 生体分子 |
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| Outline of Final Research Achievements |
Tooth and hair are formed by invagination of epithelium. Invaginated epithelium interacts with neural crest derived mesenchymal tissues, and then formed tooth and hair. However, mechanism of organ determination has never clearly understood. Sox21 is one of the transcription factor, and expressed in dental epithelium. Sox21 knockout mice showed hair formation in incisor indicating that Sox21 may be involved in the determination of organs. Decrease Sox21 inhibited ameloblast marker gene expression, and induced hair marker genes including keratins. ChipSeq analysis showed that Sox21 directly binds to Anapc10 gene promoter region and regulated their expression in dental epithelium. In Sox21 knockout mice, Anapc10 expression was decreased in tooth germ. Further, Sox21 over expression induced Anapc10 expression. These results suggested that Sox21 regulates Anapc10 expression and organ determination.
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