High sensitive bio-fluorometric gas sensors using NADH-dependent enzymes catalyzing odorous chemicals

• Project/Area Number: 26280053
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Perceptual information processing
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Mitsubayashi Kohji 東京医科歯科大学, 生体材料工学研究所, 教授 (40307236)
• Co-Investigator(Kenkyū-buntansha): 當麻 浩司 東京医科歯科大学, 生体材料工学研究所, 助教 (40732269) ; 荒川 貴博 東京医科歯科大学, 生体材料工学研究所, 講師 (50409637)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 生化学式蛍光ガスセンサ / NADH / 脱水素酵素 / 還元酵素 / 光計測 / センシングデバイス・システム

Outline of Final Research Achievements

Several biochemical gas sensors were developed using an NADH fluorometric unit with a UV-LED excitation and several dehydrogenase enzymes for high sensitive detection of odorous chemicals. As the results, the sensors allowed to monitor the concentration of volatile chemicals (nonenal by ALDH, acetaldehyde by ADH and formaldehyde by FALDH, respectively), thus showing the possibility of novel artificial nose.

Research Products

• [Journal Article] Optical isopropanol biosensor using NADH-dependent secondary alcohol dehydrogenase (S-ADH) (2016)
  • Author(s): Po-Jen Chien, Ming Ye, Takuma Suzuki, Koji Toma, Takahiro Arakawa, Yasuhiko Iwasaki, Kohji Mitsubayashi
  • Journal Title: Talanta Volume: 159 Pages: 418-424
  • DOI: 10.1016/j.talanta.2016.06.036
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] A Fluorometric Biochemical Gas Sensor (Biosniffer) for Acetaldehyde Vapor Based on Catalytic Reaction of Aldehyde Dehydrogenase (2015)
  • Author(s): Y. Suzuki, M. Ye, K. Miyajima, T. Arakawa, S. Sawada, H. Kudo, K. Akiyoshi, K. Mitsubayashi
  • Journal Title: Sensors and Materials Volume: 27(11) Pages: 1123-1130
  • DOI: 10.18494/SAM.2015.1149
  • ISSN: 0914-4935, 2435-0869
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] 日常医療のためのキャビタス(体腔)センサと生体ガスのバイオ計測（光スニファ＆探嗅カメラ） (2016)
  • Author(s): 三林浩二
  • Organizer: 応用物理学会 有機分子・バイオエレクトロニクス分科会講習会
  • Place of Presentation: 東京理科大学 森戸記念館（飯田橋）
  • Year and Date: 2016-11-07
  • Invited
• [Presentation] ホルムアルデヒド用バイオスニファ（生化学式ガスセンサ）と環境浄化評価への応用 (2015)
  • Author(s): 簡 伯任、叶 明、當麻浩司、荒川貴博、工藤寛之、三林浩二
  • Organizer: 第47回化学工学会秋季大会
  • Place of Presentation: 北海道大学（北海道・札幌市）
  • Year and Date: 2015-09-09
• [Presentation] 加齢臭成分trans-2-ノネナールの気相バイオセンシングに関する研究 (2015)
  • Author(s): 森 英久，叶 明，當麻 浩司，荒川 貴博，三林 浩二
  • Organizer: 平成27年電気学会全国大会
  • Place of Presentation: 東京（東京都市大学）
  • Year and Date: 2015-03-24 – 2015-03-26
• [Presentation] Highly sensitive fiber-optic formaldehyde gas sensor (biosniffer) for food-sample detection (2014)
  • Author(s): Jen Chien P, Miyajima K, Munkhjargal M, Toma K, Arakawa T, Kudo H, Mitsubayashi K
  • Organizer: International Conference on BioSensors, BioElectronics, BioMedical Devices, BioMEMS/NEMS and Applications (Bio4Apps) 2014
  • Place of Presentation: Shanghai, China
  • Year and Date: 2014-11-17 – 2014-11-19