| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
|
|---|
| Outline of Final Research Achievements |
Pseudocentrotus depressus was exposed to 400 (control), 2000 and 10,000 ppm CO2 for 48 days. Acclimation to higher CO2 conditions reduced contraction force of the tube feet. Two-dimensional gel electrophoresis showed that eight spots changed in protein volume. Using matrix-assisted laser desorption/ionization-quadrupole ion trap-time of flight mass spectrometry, three upregulated spots (tubulin beta chain, tropomyosin fragment, and actin N-terminal fragment) and two downregulated spots (actin C-terminal fragment and myosin light chain) were identified. Hemicentrotus pulcherrimus was used to study separate and combined effects of a 9-month exposure to 1,000 ppm CO2 and temperature (2°C above ambient).The sea urchins in elevated CO2 showed a gradual decline in food intake. Spawning did not occur in both high CO2 conditions. Temperature elevation alone caused earlier spawning. The projected changes in seawater conditions would disrupt physiological traits and reproduction of sea urchins.
|
