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Improved detection of atmospheric bioaerosols by integrating online fluorescence method with offline measurements based on DNA staining

Research Project

Project/Area Number 26281018
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Environmental dynamic analysis
Research InstitutionJapan Agency for Marine-Earth Science and Technology

Principal Investigator

KANAYA Yugo  国立研究開発法人海洋研究開発機構, 地球表層物質循環研究分野, 分野長代理 (60344305)

Co-Investigator(Kenkyū-buntansha) 宮川 拓真  国立研究開発法人海洋研究開発機構, 地球表層物質循環研究分野, 研究員 (30707568)
竹谷 文一  国立研究開発法人海洋研究開発機構, 地球表層物質循環研究分野, 主任研究員 (50377785)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Keywords大気現象 / 生物起源有機エアロゾル / PM2.5 / 蛍光法 / DNA / 生物起源エアロゾル
Outline of Final Research Achievements

Organic particles consist 20-50% of PM2.5 aerosol mass concentrations in the Earth's atmosphere. However, their origins are mostly not well identified. In this study, criteria to identify bioaerosols, a possibly important class of unidentified organics, were established for an online sensor detecting autofluorescence (WIBS4), by comparison to a more reliable, offline measurement with DNA staining. Specifically, comparison measurements at Fukue Island, western Japan, and those during three research cruises on R/V Mirai were conducted and the results were comprehensively analyzed. Optimization of the criteria was made separately for terrestrial and marine origin air masses. We also found that bioaerosols from Siberian forest fires even reached open ocean on the Bering Sea, and also that marine atmospheric bioaerosol concentrations could depend on wind speed and SST, in addition to their corresponding concentrations in the surface seawater.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (15 results)

All 2017 2016 2015 2014

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Journal Article] Intercomparison between a single particle soot photometer and evolved gas analysis in an industrial area in Japan: Implications for the consistency of soot aerosol mass concentration measurements2016

    • Author(s)
      T. Miyakawa, Y. Kanaya, Y. Komazaki, F. Taketani, X. Pan, M. Irwin, J. Symonds
    • Journal Title

      Atmospheric Environment

      Volume: 127 Pages: 14-21

    • DOI

      10.1016/j.atmosenv.2015.12.018

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Analysis of the mixing State of airbone particles using a tandem combination of laser-induced fluorescence and incandescence techniques2015

    • Author(s)
      F. Taketani, Y. Kanaya, T. Nakamura, N. Takeda, K. Koizumi, N. Hirayama, T. Miyakawa, X. Pan, N. Moteki, N. Takegawa
    • Journal Title

      Journal of Aerosol Science

      Volume: 87 Pages: 102-108

    • DOI

      10.1016/j.jaerosci.2015.05.002

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 観測とモデルからPM2.5越境大気汚染の実像に迫る2017

    • Author(s)
      金谷有剛
    • Organizer
      福井県衛生環境研究センタ―研修会
    • Place of Presentation
      福井県衛生環境研究センター、福井県福井市
    • Year and Date
      2017-02-27
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Ozone and aerosol studies over Japan: Perspectives of continental outflow and megacity pollution2017

    • Author(s)
      Yugo Kanaya
    • Organizer
      topical workshop TEAM: Transport and transformation of pollutants from European and Asian Major Population Centres
    • Place of Presentation
      Univ. Bremen, Bremen, Germany
    • Year and Date
      2017-02-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 北極海、ベーリング海、西部北太平洋上でのオンライン計測による蛍光性粒子解析2016

    • Author(s)
      竹谷文一、宮川拓真、駒崎雄一、高島久洋、金谷有剛
    • Organizer
      第22回大気化学討論会
    • Place of Presentation
      北海道大学、北海道札幌市
    • Year and Date
      2016-10-12
    • Related Report
      2016 Annual Research Report
  • [Presentation] 有機粒子の未把握起源に関する研究:蛍光性バイオエアロゾルなど2016

    • Author(s)
      金谷有剛、竹谷文一、宮川拓真
    • Organizer
      北海道大学低温研研究集会:有機エアロゾルに関する大気化学的研究:組成、起源、および光化学的変質
    • Place of Presentation
      北海道大学、北海道札幌市
    • Year and Date
      2016-08-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] レーザー誘起蛍光―白熱法を利用した大気粒子の混合状態解析2016

    • Author(s)
      竹谷文一、金谷有剛、中村貴之、武田直希、小泉和裕、宮川拓真、Xiaole Pan, 茂木信宏、平山紀友、竹川暢之
    • Organizer
      日本地球惑星科学連合2016年大会
    • Place of Presentation
      幕張メッセ、千葉県千葉市
    • Year and Date
      2016-05-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 北極海、ベーリング海、西部北太平洋上における蛍光性粒子測定2016

    • Author(s)
      竹谷文一、宮川拓真、金谷有剛、駒崎雄一、高島久洋
    • Organizer
      ブルーアース2016
    • Place of Presentation
      東京海洋大学、東京都品川区
    • Year and Date
      2016-03-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] Online measurements for fluorescent airborne particles at Fukue island in fall 20112015

    • Author(s)
      Fumikazu Taketani,Kohei Ikeda, Yugo Kanaya
    • Organizer
      American Association for Aerosol Research (AAAR) 34th Annual Conference
    • Place of Presentation
      Hyatt Regency Minneapolis, Minneapolis, Minnesota, USA
    • Year and Date
      2015-10-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 2011年秋季における長崎県福江島における蛍光性粒子のオンライン測定2015

    • Author(s)
      竹谷文一、池田恒平、金谷有剛
    • Organizer
      第56回大気環境学会年会
    • Place of Presentation
      早稲田大学、東京都新宿区
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] Synthesis of observations of atmospheric black carbon, organics and PM2.5 aerosols over Japan, Korea, and China with numerical simulations to better constrain their emission rates and origins2015

    • Author(s)
      Yugo Kanaya, Fumikazu Taketani, Xiaole Pan, Yuichi Komazaki, Takuma Miyakawa, Hitoshi Matsui, Kazuyo Yamaji, Zifa Wang, Limseok Chang, Daeil Kang, Akinori Takami, Kohei Ikeda, Hiroshi Tanimoto, Yutaka Kondo
    • Organizer
      AOGS 12th Annual Meeting
    • Place of Presentation
      Suntec国際会議場, Singapore
    • Year and Date
      2015-08-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] グリオキザール(CHOCHO, GLY)経由のSOA生成に関する知見2015

    • Author(s)
      金谷有剛
    • Organizer
      日本における地球大気化学の将来検討会~不均一反応研究の現状と今後~
    • Place of Presentation
      KKR熱海、静岡県熱海市
    • Year and Date
      2015-01-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] 植生起源イソプレンの放出量と大気酸化反応と生成物に関する知見2014

    • Author(s)
      金谷有剛
    • Organizer
      研究集会「寒冷圏陸域植生と大気微粒子・気体成分を介した大気環境の相互作用」
    • Place of Presentation
      北海道大学低温科学研究所、北海道札幌市
    • Year and Date
      2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 日中韓広域大気汚染集中観測における有機エアロゾル・PM2.5濃度のモデル再現性評価:VBSアプロー チによる改善2014

    • Author(s)
      金谷有剛、松井仁志、竹谷文一、駒崎雄一、Xiaole Pan、山地一代、池田恒平、高見昭憲、谷本浩志、 近藤豊、Zifa Wang、Daeil Kang、Limseok Chang
    • Organizer
      第20回大気化学討論会
    • Place of Presentation
      府中グリーンプラザ、東京都府中市
    • Year and Date
      2014-10-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Mass concentrations of organic aerosol and PM2.5 in East Asia reproduced by using volatility base-set approach in the WRF-Chem model2014

    • Author(s)
      Yugo Kanaya, Hitoshi Matsui, Fumikazu Taketani, Xiaole Pan, Yuichi Komazaki, Kohei Ikeda, Kazuyo Yamaji, Zifa Wang, Limseok Chang, Daeil Kang, Akinori Takami, Hiroshi Tanimoto, and Yutaka Kondo
    • Organizer
      13th Quadrennial ICACGP Symposium & 13th IGAC Science Conference on Atmospheric Chemistry
    • Place of Presentation
      Natal Convention Center, Natal, Brazil
    • Year and Date
      2014-09-25
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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