| Budget Amount *help |
¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Outline of Final Research Achievements |
We successfully designed and integrated a human derived estrogen biosensor construct into the Daphnia magna genome. The resulting line expresses functional human estrogen alpha and its reporter gene exhibits fluorescence responses to exposure to both natural and synthetic estrogens as tested with E2 and DES. Sensitivity must be improved for practical application for environmental monitoring, either by changing the exposure javascript:onTransientSave()regiment or the genetic cssette, e.g. by replacement of mCherry with a brighter fluorescent protein like tdTomato, or the introduction of human coactivators like SRC-1, SRC-3 and LRP16. Nevertheless, human estrogen receprot could be shown to be functional in daphnia for the first time, suggesting a huge potential for the use of daphnia to study the interaction of human genes with environmental factors like the effect of EDCs in this study.
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