Introduction of human nuclear receprors into Daphnia for chemical monitoring of water environment
| Project/Area Number |
26281027
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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| Keywords | ミジンコ / 緑色蛍光タンパク質 / エストロゲン / エストロゲン受容体 / バイオモニタリング / レポーター遺伝子 / ゲノム編集 / バイオアッセイ / 化学物質影響 / 環境指標生物 / 蛍光タンパク質 / ホルモン |
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| Outline of Final Research Achievements |
We successfully designed and integrated a human derived estrogen biosensor construct into the Daphnia magna genome. The resulting line expresses functional human estrogen alpha and its reporter gene exhibits fluorescence responses to exposure to both natural and synthetic estrogens as tested with E2 and DES. Sensitivity must be improved for practical application for environmental monitoring, either by changing the exposure javascript:onTransientSave()regiment or the genetic cssette, e.g. by replacement of mCherry with a brighter fluorescent protein like tdTomato, or the introduction of human coactivators like SRC-1, SRC-3 and LRP16. Nevertheless, human estrogen receprot could be shown to be functional in daphnia for the first time, suggesting a huge potential for the use of daphnia to study the interaction of human genes with environmental factors like the effect of EDCs in this study.
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Report
(4 results)
Research Products
(55 results)
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[Journal Article] In Vitro Membrane Protein Synthesis Inside Cell-Sized Vesicles Reveals the Dependence of Membrane Protein Integration on Vesicle Volume.2014
Author(s)
Soga, H., Fujii, S., Yomo, T., Kato, Y., Watanabe, H. and Matsuura, T.
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Journal Title
ACS Synth Biol
Volume: 0
Pages: 16796-16801
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Molecular impact of juvenile hormone agonists on neonatal Daphnia magna.2014
Author(s)
Toyota, K., Kato, Y., Miyakawa, H., Yatsu, R., Mizutani, T., Ogino, Y., Miyagawa, S., Watanabe, H., Nishide, H., Uchiyama, I., Tatarazako, N. and Iguchi, T.
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Journal Title
Jounal of Applied Toxicology
Volume: 34
Pages: 537-544
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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