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Effects of nitrous acid exposure on base line pulmonary resistance and inflammation in rats

Research Project

Project/Area Number 26281034
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Environmental impact assessment
Research InstitutionOsaka Prefectural Institute of Public Health

Principal Investigator

Ohyama Masayuki  大阪府立公衆衛生研究所, 衛生化学部, 主任研究員 (40175253)

Co-Investigator(Kenkyū-buntansha) 東 賢一  近畿大学, 医学部, 准教授 (80469246)
竹中 規訓  大阪府立大学, 工学(系)研究科(研究院), 教授 (70236488)
礒濱 洋一郎  東京理科大学, 薬学部, 教授 (10240920)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Keywords大気汚染 / 喘息 / 亜硝酸 / 二酸化窒素 / 肺抵抗 / ラット / 肺気腫 / モルモット / 大気汚染物質 / TNF-α / Muc5ac / アレルギー・ぜんそく / 環境 / 衛生 / 社会医学 / 特異的気道抵抗 / 呼吸機能 / 動物曝露実験
Outline of Final Research Achievements

The relationship between nitrogen dioxide (NO2) and asthma has been studied in many epidemiological studies; however, conventional assays of NO2 measure nitrous acid (HONO) as NO2. We investigated the effects of HONO on base line pulmonary resistance (RLung), dynamic lung compliance (Cdyn), cytokine induction, and histopathological changes in rats.
Our results demonstrated that HONO exposure significantly induced an increase in RLung and Muc5ac expression but did not affect Cdyn or expression of Cxcl-1 and TNF-a, and that the Lm of HONO exposure groups was significantly longer than that of control group. We found hypertrophy of the bronchial smooth muscle, pulmonary emphysema-like alterations in the alveolar duct centriacinar regions, and increased mucous secretion in goblets cells in HONO-exposed rats.
The present results suggest that HONO have a strong effect on asthma, and that HONO is the cause of the relationship between NO2 and asthma by epidemiological studies.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (22 results)

All 2017 2016 2015 2014 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Remarks (5 results)

  • [Journal Article] 亜硝酸ガスのモルモット曝露による特異的気道抵抗への影響2016

    • Author(s)
      大山正幸, 東賢一, 峰島知芳,安達修一, 竹中規訓
    • Journal Title

      第43回建築物環境衛生管理全国大会抄録集

      Volume: 43 Pages: 90-91

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurements of Nitrous Acid (HONO) Direct Emission from Rice Paddy Soil and Its Contribution to Atmospheric HONO Concentration2015

    • Author(s)
      峰島 知芳,中根 令以, 島田 幸治郎, 利谷 翔平, 佐藤 啓市, 大山 正幸, 寺田 昭彦, 細見 正明
    • Journal Title

      Journal of Japan Society for Atmospheric Environment / Taiki Kankyo Gakkaishi

      Volume: 50 Issue: 6 Pages: 249-256

    • DOI

      10.11298/taiki.50.249

    • NAID

      130005144029

    • ISSN
      1341-4178, 2185-4335
    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Comparing the role of silica particle size with mineral fiber geometry in the release of superoxide from rat alveolar macrophages2014

    • Author(s)
      M. Ohyama, H. Tachi, C. Minejima, T. Kameda
    • Journal Title

      The Journal of Toxicological Sciences

      Volume: 39 Issue: 4 Pages: 551-559

    • DOI

      10.2131/jts.39.551

    • NAID

      130004904003

    • ISSN
      0388-1350, 1880-3989
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparing Heat-treated Silica Particle with Silica Particles for the Ability to Induce Superoxide Release from Rat Alveolar Macrophages2014

    • Author(s)
      M. Ohyama, H. Tachi, C. Minejima, T. Kameda
    • Journal Title

      J. Clin. Toxicol.

      Volume: 4 Issue: 03 Pages: 199-199

    • DOI

      10.4172/2161-0495.1000199

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ラット肺抵抗に対する亜硝酸曝露の影響2017

    • Author(s)
      大山正幸(代表)
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      東北大学川内北キャンパス(宮城県仙台市)
    • Year and Date
      2017-03-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Effects of nitrous acid exposure on pulmonary resistance of rats2017

    • Author(s)
      Masayuki Ohyama(代表)
    • Organizer
      第90回日本薬理学会
    • Place of Presentation
      長崎ブリックホール(長崎県長崎市)
    • Year and Date
      2017-03-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] ラット肺抵抗に対する亜硝酸曝露の影響2017

    • Author(s)
      大山正幸(代表)
    • Organizer
      黄砂等に関する共同研究会
    • Place of Presentation
      鳥取県衛生環境研究所(鳥取県東伯郡)
    • Related Report
      2016 Annual Research Report
  • [Presentation] モルモット特異的気道抵抗に対する亜硝酸曝露の影響(2)2016

    • Author(s)
      大山正幸(代表)
    • Organizer
      第57回大気環境学会
    • Place of Presentation
      北海道大学工学部(北海道札幌市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] ラット呼吸機能に対する亜硝酸曝露の影響2016

    • Author(s)
      大山正幸(代表)
    • Organizer
      第57回大気環境学会
    • Place of Presentation
      北海道大学工学部(北海道札幌市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
  • [Presentation] モルモット曝露実験における特異的気道抵抗と亜硝酸との関係2016

    • Author(s)
      大山正幸, 東賢一, 峰島知芳,安達修一, 竹中規訓
    • Organizer
      日本薬学会第136年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-03-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Effects of Nitrous Acid Exposure on Specific Airway Resistance of Guinea Pigs2016

    • Author(s)
      Masayuki Ohyama, Hiroshi Nishimura, Shuichi Adachi, Kenji Oka, Kenichi Azuma, Chika Minejima, Norimichi Takenaka
    • Organizer
      第89回日本薬理学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-03-09
    • Related Report
      2015 Annual Research Report
  • [Presentation] 喘息影響指標である特異的気道抵抗に与える亜硝酸の影響2016

    • Author(s)
      大山正幸, 東賢一, 峰島知芳,安達修一, 竹中規訓
    • Organizer
      平成27年度 黄砂共同連絡会
    • Place of Presentation
      大阪府立環境農林水産総合研究所
    • Year and Date
      2016-02-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 硫酸アンモニウムの人体吸入実験の論文紹介2016

    • Author(s)
      大山正幸
    • Organizer
      大気環境学会近畿支部人体影響部会2016年度セミナー
    • Place of Presentation
      大阪府立環境農林水産総合研究所(大阪府大阪市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Nitrous Acid induces Pulmonary Emphysema-like Alterations in Guinea Pigs2015

    • Author(s)
      Masayuki Ohyama, Hiroshi Nishimura, Shuichi Adachi, Kenji Oka, Kenichi Azuma, Chika Minejima, Norimichi Takenaka
    • Organizer
      The international conference on the Atmospheric Sciences and Application to Air Quality (ASAAQ)
    • Place of Presentation
      Kobe International Conference Center.
    • Year and Date
      2015-11-11
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] モルモット特異的気道抵抗に対する亜硝酸曝露の影響2015

    • Author(s)
      大山正幸, 東賢一, 峰島知芳,安達修一, 竹中規訓
    • Organizer
      第56回大気環境学会
    • Place of Presentation
      早稲田大学西早稲田キャンパス
    • Year and Date
      2015-09-15
    • Related Report
      2015 Annual Research Report
  • [Presentation] 粉塵のマクロファージ活性酸素反応亢進因子-形状や物質-2015

    • Author(s)
      大山正幸
    • Organizer
      黄砂に関する共同研究会
    • Place of Presentation
      大阪
    • Year and Date
      2015-02-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] Guessing the fossil fuel mix used at emission sources from a downwind location2014

    • Author(s)
      C. Minejima, Y. Tohjima, M. Kubo, H. Mukai, H. Yamagishi,K. Kita, Y. Koyama, S. Maksyutov, R. Nakane, K. Shimada, S. Riya, K. Sato, M. Ohyama, M. Hosomi
    • Organizer
      14th Japanese-American Frontiers of Science Symposium
    • Place of Presentation
      東京
    • Year and Date
      2014-10-04 – 2014-10-07
    • Related Report
      2014 Annual Research Report
  • [Remarks] 大気中に存在するガス状亜硝酸について

    • URL

      http://www.iph.pref.osaka.jp/merumaga/back/145-2.html

    • Related Report
      2015 Annual Research Report
  • [Remarks] 亜硝酸ガスと喘息症状の関連性に関する疫学的事例調査

    • URL

      http://www.iph.pref.osaka.jp/news/vol53/news53_1.html

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 大気中に存在する亜硝酸の生体影響

    • URL

      http://www.iph.pref.osaka.jp/news/vol43/news43_2.html

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 大気中に存在する亜硝酸の生体影響について

    • URL

      http://www.iph.pref.osaka.jp/merumaga/back/105-2.html

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] 大阪とソウルに飛来した黄砂に対するマクロファージの活性酸素反応の違い

    • URL

      http://www.iph.pref.osaka.jp/merumaga/back/104-2.html

    • Related Report
      2014 Annual Research Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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