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Formation and degradation of solid-phase humic substances as electron mediator for pollutant-degrading microorganisms in geosphere

Research Project

Project/Area Number 26281040
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Modeling and technologies for environmental conservation and remediation
Research InstitutionNagoya University

Principal Investigator

Katayama Arata  名古屋大学, 未来材料・システム研究所, 教授 (60185808)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords腐植物質 / 細胞外電子伝達 / 脱塩素菌 / 固体腐植ヒューミン / 金属-腐植酸複合体 / ペンタクロロフェノール / 嫌気性脱塩素反応 / 細胞外電子伝達系 / 脱ハロゲン呼吸 / 固体腐植 / 硝酸還元呼吸 / 腐植生成消失 / 細胞外電子伝達物質 / 固体腐植物質 / メラノイジン反応 / 金属ー腐植酸複合体 / ペンタクロロフェノール脱塩素活性 / 嫌気性微生物 / 還元的脱塩素反応 / 硝酸還元 / 電気培養 / 機能解析 / 反応中心解析 / 腐植化
Outline of Final Research Achievements

Because of the persistent characteristics of humic substances in the environment, they are not considered as active material in biological metabolic systems. However, humic substances are now understood as external electron mediators for microbes. This study aims to investigate the formation and degradation of humic substances as external electron mediators in natural environment. To examine the function of external electron mediator, we have used the humin-dependent anaerobic pentachlorophenol-dechlorinating culture. The results showed that the freeze-dried powder of some bacteria and fungi functioned as external electron mediators, and that some products of humic acids and metals showed the external electron mediators. These suggested the formation and degradation of external electron mediators in natural environment.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (25 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (8 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 8 results,  Acknowledgement Compliant: 8 results,  Open Access: 1 results) Presentation (14 results) (of which Int'l Joint Research: 8 results,  Invited: 3 results) Remarks (1 results)

  • [Int'l Joint Research] ハルピン工科大学/浙江大学(中国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 中国地質大学/哈爾浜工業大学/浙江大学(中国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Biofilm anode involved Geobacter in microbial fuel cell for phenol degradation2017

    • Author(s)
      Dongdong Zhang, Zhiling Li, Chunfang Zhang, Xue Zhou, Zhixing Xiao, Takanori Awata, Arata Katayama
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 123 Issue: 3 Pages: 364-369

    • DOI

      10.1016/j.jbiosc.2016.10.010

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Enhanced denitrification of Pseudomonas stutzeri by a bioelectrochemical system assisted with solid-phase humin2016

    • Author(s)
      Zhixing Xiao, Takanori Awata, Dongdong Zhang, Chunfang Zhang, Zhiling Li, and Arata Katayama
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 122 Issue: 1 Pages: 85-91

    • DOI

      10.1016/j.jbiosc.2015.11.004

    • NAID

      40020885930

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Denitrification of Pseudomonas stutzeri coupled with CO2 reduction by Sporomusa ovata with hydrogen as electron donor assisted with solid-phase humin2016

    • Author(s)
      Zhixing Xiao, Takanori Awata, Dongdong Zhang, and Arata Katayama
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 122 Issue: 3 Pages: 307-313

    • DOI

      10.1016/j.jbiosc.2016.02.002

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Spatial Abundance and Distribution of Potential Microbes and Functional Genes Associated with Anaerobic Mineralization of Pentachlorophenol in a Cylindrical Reactor2016

    • Author(s)
      Zhiling Li, Jun Nan, Cong Huang, Bin Liang, Wenzong Liu, Haoyi Cheng, Chunfang Zhang, Dongdong Zhang, Deyong Kong, Kyoko Kanamaru, Tetsuo Kobayashi, Aijie Wang, and Arata Katayama
    • Journal Title

      Scientific Report

      Volume: 6 Issue: 1 Pages: 19015-19015

    • DOI

      10.1038/srep19015

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Polyphasic characterization of an anaerobic hexachlorobenzene-dechlorinating microbial consortium with a wide dechlorination spectrum for chlorobenzenes2015

    • Author(s)
      Xue Zhou, Chunfang Zhang, Dongdong Zhang, Takanori Awata, Zhixing Xiao, Qi Yang, Arata Katayama
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 120 Issue: 1 Pages: 62-68

    • DOI

      10.1016/j.jbiosc.2014.11.023

    • NAID

      110009973287

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Temporal distributions of functional microbes and putative genes associated with halogenated phenol anaerobic dehalogenation and further mineralization2015

    • Author(s)
      Zhiling Li, Jun Nan, Jiaqi Yang, Xiao Jin, Arata Katayama, Aijie Wang
    • Journal Title

      RSC Advances

      Volume: 5 Issue: 108 Pages: 89157-89163

    • DOI

      10.1039/c5ra16815e

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Characterization of humins from different natural sources and the effect on microbial reductive dechlorination of pentachlorophenol2015

    • Author(s)
      Chunfang Zhang, Dongdong Zhang, Zhixing Xiao, Zhiling Li, Daisuke Suzuki, Arata Katayama
    • Journal Title

      Chemosphere

      Volume: 131 Pages: 110-116

    • DOI

      10.1016/j.chemosphere.2015.02.043

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Enhancement of multiple microbial reduction reactions with different redox potentials in a consortium by solid-phase humin as a redox mediator2014

    • Author(s)
      99.Dongdong Zhang, Chunfang Zhang, Zhixing Xiao, Daisuke Suzuki, Arata Katayama
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 119 Issue: 2 Pages: 188-194

    • DOI

      10.1016/j.jbiosc.2014.07.010

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Design of artificial anaerobic microbial community for bioremediation2016

    • Author(s)
      Arata Katayama
    • Organizer
      the 2nd International Conference on Systems & Synthetic Biology
    • Place of Presentation
      London, UK
    • Year and Date
      2016-08-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Electron Transfer assisted by solid-phase electron mediator (humin) in bioelectrochemical system for enhancing anaerobic bioremediation2016

    • Author(s)
      D. Zhang, Z. Xiao, T. Awata, A. Katayama
    • Organizer
      Second International symposium on Advanced Water Science and Technology (ISAWST-2)
    • Place of Presentation
      Nagoya University, Nagoya, Japan
    • Year and Date
      2016-02-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Solid-phase humin: A “new” natural material with redox mediating property for the anaerobic biotransformation of pollutants2016

    • Author(s)
      Z. Xiao, D. Zhang, C. Zhang, T. Awata, A. Katayama
    • Organizer
      Second International symposium on Advanced Water Science and Technology (ISAWST-2)
    • Place of Presentation
      Nagoya University, Nagoya, Japan
    • Year and Date
      2016-02-15
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Enhancement of Different Microbial Reduction Reactions by Humin as a Versatile Electron donor2015

    • Author(s)
      D. Zhang, C. Zhang, Z. Xiao, D. Suzuki, A. Katayama
    • Organizer
      International Symposium on EcoTopia Science 2015 - Innovation for Smart Sustainable Society -
    • Place of Presentation
      Nagoya University, Nagoya, Japan
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characteristics of humin as solid-phase electron mediator2015

    • Author(s)
      Arata Katayama, Chunfang Zhang
    • Organizer
      International Symposium on EcoTopia Science 2015 - Innovation for Smart Sustainable Society -
    • Place of Presentation
      Nagoya University, Nagoya, Japan
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The Development of the Environmental Depollution Electrochemical Device using a Microbial Electrode2015

    • Author(s)
      Y. Miyata, K. Kanda, T. Awata, A. Katayama
    • Organizer
      International Symposium on EcoTopia Science 2015 - Innovation for Smart Sustainable Society -
    • Place of Presentation
      Nagoya University, Nagoya, Japan
    • Year and Date
      2015-11-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biofilm anode involving Geobacter for phenol degradation and electricity production2015

    • Author(s)
      Dongdong Zhang, Zhiling Li, Chunfang Zhang, Xue Zhou, Zhixing Xiao, Takanori Awata, Arata Katayama
    • Organizer
      The 5th International Meeting on Microbial Electrochemistry and Technologies
    • Place of Presentation
      Arizona State University, Tempe, Arizona, USA
    • Year and Date
      2015-10-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Humin-assisted bioelectrochemical system for the enhanced denitrification of Pseudomonas stutzeri (JCM20778)2015

    • Author(s)
      Zhixing Xiao, Awata Takanori, Dongdong Zhang,Chunfang Zhang, Zhiling Li, and Arata Katayama
    • Organizer
      The 5th International Meeting on Microbial Electrochemistry and Technologies
    • Place of Presentation
      Arizona State University, Tempe, Arizona, USA
    • Year and Date
      2015-10-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Enhancement of anaerobic microbial dehalogenation by extracellular electron transfer2014

    • Author(s)
      rata Katayama, Chunfang Zhang, Dongdong Zhang, Zhixing Xiao, & Takanori Awata
    • Organizer
      World Green Energy & Resources Congress 2014
    • Place of Presentation
      Beijing, China
    • Year and Date
      2014-11-01 – 2014-11-03
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Electrochemically-mediated microbial dehalogenation of halogenated aromatics2014

    • Author(s)
      ongdong Zhang, Cunfang Zhang, Zhixing Xiao, Daisuke Suzuki, and Arata Katayama
    • Organizer
      The 11th International Symposium on Persistent Toxic Substances
    • Place of Presentation
      City University of Hong Kong, Hong Kong
    • Year and Date
      2014-10-27 – 2014-10-30
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 細胞外電子伝達として機能する固体腐植ヒューミンによる嫌気脱ハロゲン微生物の活性化2014

    • Author(s)
      章春芳、張冬冬、肖智興、鈴木大典、粟田貴宣、片山新太
    • Organizer
      環境微生物系合同大会
    • Place of Presentation
      浜松アクトシティコングレスセンター、浜松
    • Year and Date
      2014-10-21 – 2014-10-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Anaerobic bioremediation technology for halogenated aromatic compounds using multiple microbial communities in combination2014

    • Author(s)
      Arata Katayama
    • Organizer
      SETAC Asia 2014
    • Place of Presentation
      Adelaide, South Australia
    • Year and Date
      2014-09-14 – 2014-09-17
    • Related Report
      2014 Annual Research Report
  • [Presentation] Solid-phase humin-assisted microbial dehalogenation of aromatic compounds under anaerobic conditions2014

    • Author(s)
      Arata Katayama, Chunfang Zhang
    • Organizer
      13th IUPAC International Congress of Pesticide Chemistry
    • Place of Presentation
      San Francisco, CA, USA
    • Year and Date
      2014-08-10 – 2014-08-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Humin based bio-electrochemical system for nitrate reduction using pure culture of Pseudomonas stutzeri and Shewanella CN322014

    • Author(s)
      Xhixing XIAO, Dongdong ZHANG, Chunfang ZHANG, Daisuke SUZUKI, and Arata KATAYAMA
    • Organizer
      Water and Environment Technology Conference
    • Place of Presentation
      TWIns, Waseda University
    • Year and Date
      2014-06-28 – 2014-06-30
    • Related Report
      2014 Annual Research Report
  • [Remarks] 片山新太研究室「微生物による環境修復と資源化」

    • URL

      http://www.er.imass.nagoya-u.ac.jp/AKLab_J/

    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2022-03-01  

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