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Microbial production of elastic polymers with ordered structure

Research Project

Project/Area Number 26281043
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Environmental conscious materials and recycle
Research InstitutionHokkaido University

Principal Investigator

Matsumoto Ken'ichiro  北海道大学, 工学(系)研究科(研究院), 准教授 (80360642)

Co-Investigator(Kenkyū-buntansha) 大井 俊彦  北海道大学, 工学(系)研究科(研究院), 准教授 (40223713)
田口 精一  北海道大学, 工学(系)研究科(研究院), 教授 (70216828)
佐藤 敏文  北海道大学, 工学(系)研究科(研究院), 教授 (80291235)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2015: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2014: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Keywordsバイオベースプラスチック / 微生物合成 / 生分解性材料 / 高分子合成 / 酵素反応 / バイオベースポリマー / 酵素重合 / ポリエステル / バイオプラスチック
Outline of Final Research Achievements

Microbial polyesters are useful material, which are produced from renewable biomass. For the practical use of the material, polymer properties are critical factors. It is well-known that microbial copolyesters are random polymer. However, we found a condition that generated copolymer with low randomness. This study addressed optimization of the polymer production, structure and property analysis, and kinetic analysis of relevant enzymes. As the results, the copolymer, in which the monomer composition was regulated, was successfully synthesized with good yield. The copolymers exhibit transparent and flexible properties. The enzymatic activity of relevant enzymes accounted for the intracellular intermediate levels. Overall, the study contributed to the significantly improved the efficiency of the polymer production system, and provided insight into the mechanism of the polymer synthesis.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Annual Research Report
  • 2014 Annual Research Report
  • Research Products

    (10 results)

All 2017 2016 2015 2014

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Acknowledgement Compliant: 5 results,  Open Access: 2 results) Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Incorporation of glycolate units promotes hydrolytic degradation in flexible poly(glycolate-co-3-hydroxybutyrate) synthesized by engineered Escherichia coli2016

    • Author(s)
      K. Matsumoto, T. Shiba, Y. Hiraide, S. Taguchi
    • Journal Title

      ACS Biomaterials Science & Engineering

      Volume: in press Issue: 12 Pages: 3058-3063

    • DOI

      10.1021/acsbiomaterials.6b00194

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Microbial Secretion of D-Lactate-Based Oligomers2016

    • Author(s)
      C. Utsunomia, K. Matsumoto, S. Taguchi
    • Journal Title

      ACS Sustainable Chemistry & Engineering

      Volume: in press Issue: 3 Pages: 2360-2367

    • DOI

      10.1021/acssuschemeng.6b02679

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Microbial production of poly(lactate-co-3-hydroxybutyrate) from hybrid Miscanthus-derived sugars2016

    • Author(s)
      J. Sun, C. Utsunomia, S. Sasaki, K. Matsumoto, T. Yamada, T. Ooi, S. Taguchi
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 80 Issue: 4 Pages: 818-820

    • DOI

      10.1080/09168451.2015.1116933

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular weight-dependent degradation of D-lactate-containing polyesters by polyhydroxyalkanoate depolymerases from Variovorax sp. C34 and Alcaligenes faecalis T12015

    • Author(s)
      Jian Sun, Ken’ichiro Matsumoto, Yuta Tabata, Ryosuke Kadoya, Toshihiko Ooi, Hideki Abe, Seiichi Taguchi
    • Journal Title

      Appl. Microbiol. Biotechnol.

      Volume: 99 Issue: 22 Pages: 9555-9563

    • DOI

      10.1007/s00253-015-6756-1

    • NAID

      120005855945

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase2015

    • Author(s)
      T. Yokoo, K. Matsumoto, J. M. Nduko, T. Ooi, S. Taguchi
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 3 Issue: 6 Pages: 1-3

    • DOI

      10.1080/09168451.2014.1002448

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improved production of poly(lactic acid)-like polyester based on metabolite analysis to address the rate-limiting step2014

    • Author(s)
      Ken’ichiro Matsumoto, Kota Tobitani, Shunsuke Aoki, Yuyang Song, Toshihiko Ooi, Seiichi Taguchi
    • Journal Title

      AMB Express

      Volume: 4 Issue: 1 Pages: 83-83

    • DOI

      10.1186/s13568-014-0083-2

    • NAID

      120005649137

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 非天然ポリエステル合成のための重合酵素の高活性化2017

    • Author(s)
      松本謙一郎、大石健太、大井俊彦、田口精一
    • Organizer
      日本農芸化学会大会
    • Place of Presentation
      京都女子大学(京都市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] Chiral synthesis production of poly[(R)-2-hydroxybutyrate-co-(R)-3-hydroxybutyrate] from racemic precursors using engineered polymerizing enzyme with strict enantiospecificity2017

    • Author(s)
      R. Fujii, M. Takaya, T. Ooba, K. Matsumoto, T. Ooi, S. Taguchi
    • Organizer
      National Taipei University of Technology-Hokkaido University students joint symposium in 2017
    • Place of Presentation
      Taipei University (Taipei)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microbial synthesis of P(glycolate-co-3-hydroxybutyrate) with hydrolytic degradability2015

    • Author(s)
      K. Matsumoto, T. Shiba1, Y. Hiraide, S. Taguchi:
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii Convention Center, Honolulu, Hawaii
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 組換え大腸菌によるグリコール酸ベースポリマー合成とポリマーの物性評価2015

    • Author(s)
      平出幸和、斯波哲史、孫健、大井俊彦、田口精一、松本謙一郎
    • Organizer
      農芸化学会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-27 – 2015-03-29
    • Related Report
      2014 Annual Research Report

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Published: 2014-04-04   Modified: 2018-12-17  

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