Sophistication of local gene expression microscopy by adaptive optics
| Project/Area Number |
26282128
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
KAMEI Yasuhiro 基礎生物学研究所, 生物機能解析センター, 特任准教授 (70372563)
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| Co-Investigator(Renkei-kenkyūsha) |
TAMADA Yosuke 基礎生物学研究所, 生物進化研究部門, 助教 (50579290)
HATTORI Masayuki 基礎生物学研究所, 生物機能解析センター, 研究員 (20308208)
HAYANO Yutaka 国立天文台, 先端技術センター, 准教授 (80390623)
OYA Shin 国立天文台, TMT推進室, 特任准教授 (80399287)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2015: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2014: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | 顕微鏡 / 赤外レーザー / 熱ショック / 補償光学 / 遺伝子発現 / 遺伝子機能解析 / 個体 / 温度 / 温度計測 / 光操作 / レーザー / in vivo / 蛍光タンパク質 / 細胞内温度計測 / IR-LEGO / 波面センサー |
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| Outline of Final Research Achievements |
Current concept for development of biological microscopy supposes ideal sample that constitutes with homogeneous media for optics. A real living tissue, however, is constituted with heterogeneous media that have different refractive index for optics and they disturb wave front, and finally, an image in deeper area becomes blur. The current concept could not avoid this disturbance. Therefore, new concept is required for biological microscopy. In this project, we apply adaptive optics into microscopy to improve image blurring in deeper area in living tissues. And then, we also apply this technology to new microscope method that enables local gene expression by using heat shock response, called IR-LEGO. Temperature sensing method in living organisms is also required because the IR-LEGO uses invisible infrared light. It means improvement should be evaluated by 3D temperature mapping.
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Grana-localized proteins, RIQ1 and RIQ2, affect the organization of light-harvesting vomplex II and grana stacking in Arabidopsis.2016
Author(s)
Ryo Yokoyama, Hiroshi Yamamotoa, Maki Kondo, Satomi Takeda, Kentaro Ifuku, Yoichiro Fukao, Yasuhiro Kamei, Mikio Nishimura,Toshiharu Shikanai
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Journal Title
Plant Cell
Volume: 28
Issue: 9
Pages: 2261-2275
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Application of local gene induction by infrared laser-mediated microscope and temperature stimulator to amphibian regeneration study.2015
Author(s)
Kawasumi, A., Hayashi, T., Kobayashi, T., Nagayama, C., Hayashi, S., Kamei, Y., Morishita, Y., T Takeuchi, T., Tamura, K., Yokoyama, H.
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Journal Title
Dev. Growth Differ.
Volume: 57
Issue: 9
Pages: 601-613
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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