Construction of cell-based composite tissue by using an oxygen permeable culture device

• Project/Area Number: 26282133
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tohoku University
• Principal Investigator: Anada Takahisa 東北大学, 歯学研究科(研究院), 准教授 (30398466)
• Co-Investigator(Kenkyū-buntansha): 鈴木 治 東北大学, 歯学研究科(研究院), 教授 (60374948)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 細胞・組織工学材料 / 細胞培養デバイス / 三次元培養 / 骨芽細胞 / 酸素濃度

Outline of Final Research Achievements

Engineering three-dimensional (3D) cellular constructs outside of the body is one of the most important issues for tissue engineering. Increase in size of cellular aggregates causes a depletion of oxygen in the core of aggregates. We have developed an oxygen-permeable cell culture device to prevent hypoxia in the core of the cellular aggregates. The aim of this study was construction of 3D cellular structure consisted of mesenchymal stem cells (MSCs) and vascular endothelial cells based on the oxygen-permeable device we developed. We found that the device was able to improve osteoblastic differentiation of MSCs and generate cellular aggregates consisted of two types of cells. These results suggest that the device is useful for engineering 3D cellular constructs for tissue engineering.

Research Products

• [Journal Article] A 3D culture model study monitoring differentiation of dental epithelial cells into ameloblast-like cells (2016)
  • Author(s): Tadaki, M., Anada, T., Shiwaku, Y., Nakamura, T., Nakamura, M., Kojima, M., Arai, T., Fukumoto, S., and Suzuki, O.
  • Journal Title: RSC Advances Volume: 6(67) Issue: 67 Pages: 62109-62109
  • DOI: 10.1039/c6ra04570g
  • Peer Reviewed
• [Journal Article] An oxygen-permeable spheroid culture chip (Oxy chip) promotes osteoblastic differentiation of mesenchymal stem cells (2016)
  • Author(s): T. Kamoya, T. Anada, Y. Shiwaku, T. Takano-Yamamoto, O. Suzuki
  • Journal Title: Sensors and Actuators B: Chemical Volume: 232 Pages: 75-83
  • DOI: 10.1016/j.snb.2016.03.107
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evaluation of bioactivity of octacalcium phosphate using osteoblastic cell aggregates on a spheroid culture device (2016)
  • Author(s): T. Anada, T. Sato, T. Kamoya, Y. Shiwaku, K. Tsutchiya, T. Takano-Yamamoto, K. Sasaki, O. Suzuki
  • Journal Title: Regenerative Therapy Volume: 3 Pages: 58-62
  • DOI: 10.1016/j.reth.2016.02.004
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Effect of mechanical stress on differentiation of mouse mesenchymal stem cells seeded into an octacalcium phosphate-gelatin scaffold (2015)
  • Author(s): M. Yamada, T. Anada, T. Masuda, T. Takano-Yamamoto, O. Suzuki
  • Journal Title: Sensors and Actuators B: Chemical Volume: 220 Pages: 125-130
  • DOI: 10.1016/j.snb.2015.05.073
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Characterization of partially hydrolyzed OCP crystals deposited in a gelatin matrix as a scaffold for bone tissue engineering. (2015)
  • Author(s): Ezoe Y, Anada T, Yamazaki H, Handa T, Kobayashi K, Takahashi T, Suzuki O
  • Journal Title: J Nanopart Res Volume: 17 Issue: 3 Pages: 140-140
  • DOI: 10.1007/s11051-015-2864-1
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Osteoconductive property of a mechanical mixture of octacalcium phosphate and amorphous calcium phosphate (2014)
  • Author(s): K Kobayashi, T Anada, T Handa, N Kanda, M Yoshinari, T Takahashi, O Suzuki
  • Journal Title: ACS Applied Materials & Interfaces Volume: 6 Issue: 24 Pages: 22602-22611
  • DOI: 10.1021/am5067139
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 酸素透過性三次元培養デバイスによる間葉系幹細胞培養 (2017)
  • Author(s): 穴田貴久、鈴木治
  • Organizer: 第16回日本再生医療学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-07
• [Presentation] The Effect of Three-dimensional Culture on The Ameloblastic Differentiation of Dental Epithelial Cells (2016)
  • Author(s): Anada T, Tadaki M, Shiwaku Y, Nakamura T, Nakamura M, Kojima M, Arai T, Fukumoto S, Suzuki O,
  • Organizer: 2016 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: 名古屋
  • Year and Date: 2016-11-28
  • Int'l Joint Research
• [Presentation] The application of oxygen-permeable spheroid culture chip (Oxy chip) in bone tissue engineering (2016)
  • Author(s): Anada T, Kamoya T, Sato T, Shiwaku Y, Takano-Yamamoto T, Sasaki K, and Suzuki O
  • Organizer: IDMC2016
  • Place of Presentation: バリ、インドネシア
  • Year and Date: 2016-11-04
  • Int'l Joint Research
• [Presentation] Oxygen supply to 3D cellular constructs using an oxygen-permeable culture device (2016)
  • Author(s): Anada T, Suzuki O
  • Organizer: 10th World Biomaterials Congress
  • Place of Presentation: モントリオール、カナダ
  • Year and Date: 2016-05-17
  • Int'l Joint Research
• [Presentation] MSCスフェロイドの三次元培養における酸素供給の影響 (2015)
  • Author(s): 加茂谷拓央、穴田貴久、塩飽由香利、山本照子、鈴木治
  • Organizer: 第37回日本バイオマテリアル学会
  • Place of Presentation: 京都
  • Year and Date: 2015-11-09
• [Presentation] 歯原性上皮細胞のエナメル芽細胞分化に与える三次元培養の効果 (2015)
  • Author(s): 只木麻友，穴田貴久，福本 敏，鈴木 治
  • Organizer: 65 回日本歯科理工学会学術講演会
  • Place of Presentation: 仙台市
  • Year and Date: 2015-04-11 – 2015-04-12
• [Presentation] 酸素供給型培養デバイスによるMSCスフェロイドの分化制御 (2015)
  • Author(s): 加茂谷拓央、穴田貴久、山本照子、鈴木治
  • Organizer: 第14回日本再生医療学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2015-03-20 – 2015-03-21
• [Presentation] 酸素透過性三次元細胞培養デバイスの開発と応用 (2014)
  • Author(s): 穴田 貴久、福田 淳二、鈴木 治
  • Organizer: 日本動物実験代替法学会第27回大会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-12-06 – 2014-12-07
  • Invited
• [Book] Efficacy of calcium phosphate-based scaffold materials on mineralized and non-mineralized tissue regeneration. Interface Oral Health Science (2016)
  • Author(s): O Suzuki, T Anada, Y Shiwaku
  • Total Pages: 7
  • Publisher: Springer
• [Book] 三次元ティッシュエンジニアリング―細胞の培養・操作・組織化から品質管理、脱細胞化まで (2015)
  • Author(s): 穴田貴久、福田淳二、鈴木 治
  • Total Pages: 346
  • Publisher: エヌ・ティー・エス
• [Remarks] 東北大学大学院歯学研究科顎口腔機能創建学分野ホームページ
  • URL: http://www.cfe.dent.tohoku.ac.jp/
• [Remarks] 東北大学大学院歯学研究科顎口腔機能創建学分野
  • URL: http://www.cfe.dent.tohoku.ac.jp/
• [Remarks] 東北大学大学院歯学研究科顎口腔機能創建学分野HP
  • URL: http://www.cfe.dent.tohoku.ac.jp/