| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
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| Outline of Final Research Achievements |
Polymeric hydrogel matrix was prepared from a water-soluble phospholipid polymer (MPC polymer) with high cytocompatibility. It functioned as a fine artificial extracellular matrix (ECM) to control cell functions when the cells embedding in the MPC polymer hydrogel. This artificial ECM is reversibly dissociated by the addition of a low molecular weight sugar compound. The proliferation cycle of the ES cells embedding in the hydrogel was aligned with the G0/G1 phase, and it became clear that efficiency is several times multiplied by adding the differentiation-inducing factor in this state. We also succeeded in inducing aggregates derived from one cell in the matrix. Innovative cell manipulation method/development of differentiation induction method can be developed, and along with establishment of iPS cell and ES cell preparation method, it will be developed as fundamental technology for future cell engineering and tissue regeneration medicine.
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