Development of Ischemia-Specific Oligonucleotide Therapeutics System for Acute stroke

• Project/Area Number: 26282136
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: ISHIBASHI SATORU 東京医科歯科大学, 医歯(薬)学総合研究科, 講師 (30533369)
• Co-Investigator(Kenkyū-buntansha): 和田 健彦 東北大学, 多元物質科学研究所, 教授 (20220957) ; 横田 隆徳 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (90231688)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: 脳卒中 / 核酸医薬 / 神経保護 / マウス / 人工核酸 / 環境応答型核酸 / 水素イオン指数 / 血管新生 / 脳血管障害 / 虚血応答性核酸 / ヘテロ核酸 / 神経科学 / 脳神経疾患 / 刺激応答性核酸 / 脳梗塞 / 動物モデル / pH

Outline of Final Research Achievements

Stroke remains a major cause of mortality and long-term disability worldwide. Recent studies elucidated well the complex pathogenesis of acute ischemic stroke such as dynamic alternation of genes and proteins, which could contribute to develop novel therapeutics for acute stroke.
We previously developed an heteroduplex oligonucleotide (HDO), which strongly silenced its target gene in many organs, and a peptide ribonucleic acids (PRNA), which regulated genes expression based on intracellular environmental condition (e.g. pH). By means of these two novel technologies, we aimed to create ischemia-specific oligonucleotide therapeutics system for acute stroke. In our studies, we have successfully developed a novel oligonucleotide, which could specifically and strongly inhibit gene expression in brain early in ischemic stroke and can dramatically influence its phenotype.

Research Products

• [Journal Article] Depressive disorder may be associated with raphe nuclei lesions in patients with brainstem infarction. (2017)
  • Author(s): Numasawa Y, Hattori T, Ishiai S, Kobayashi Z, Kamata T, Kotera M, Ishibashi S, Sanjo N, Mizusawa H, Yokota T
  • Journal Title: J Affect Disord. Volume: 213 Pages: 191-198
  • DOI: 10.1016/j.jad.2017.02.005
  • Peer Reviewed / Open Access
• [Journal Article] Protonation-Induced Red-Coloured Circularly Polarized Luminescence of [5]Carbohelicene Fused by Benzimidazole (2016)
  • Author(s): H. Sakai, T. Kubota, J. Yuasa, Y. Araki, T. Sakanoue, T. Takenobu, T. Wada, T. Kawai, T. Hasobe
  • Journal Title: Org. Biomol. Chem. Volume: 14 Issue: 28 Pages: 6738-6743
  • DOI: 10.1039/c6ob00937a
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Controllable Electronic Structures and Photoinduced Processes of Bay-Linked Perylenediimide Dimers and Ferrocene-Linked Triad (2016)
  • Author(s): H. Horinouchi, H. Sakai, Y. Araki, T. Sakanoue, T. Takenobu, T. Wada, N. V. Tkachenko, T. Hasobe
  • Journal Title: Chem. Eur. J. Volume: 21 Issue: 28 Pages: 9631-9641
  • DOI: 10.1002/chem.201601058
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Activation of p53 facilitates the target search in DNA by enhancing the target recognition probability (2016)
  • Author(s): Itoh Y., Murata A., Sakamoto S., Nanatani K., Wada T., Takahashi S., Kamagata K.
  • Journal Title: J. Mol. Biol. Volume: 428 Issue: 14 Pages: 2916-2930
  • DOI: 10.1016/j.jmb.2016.06.001
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Progressive Multifocal Leukoencephalopathy with Balanced CD4/CD8 T-Cell Infiltration and Good Response to Mefloquine Treatment (2016)
  • Author(s): Sanjo N, Kina S, Shishido-Hara Y, Nose Y, Ishibashi S, Fukuda T, Maehara K, Eishi Y, Mizusawa H, Yokota T
  • Journal Title: Internal Medicine Volume: 55 Issue: 12 Pages: 1631-1635
  • DOI: 10.2169/internalmedicine.55.6051
  • NAID: 130005157669
  • ISSN: 0918-2918, 1349-7235
  • Peer Reviewed / Open Access
• [Journal Article] Yoshihisa Inoue - A Researcher's Quest for Photochirogenesis (2016)
  • Author(s): Mori, T.; Fukuhara, G.; Wada, T.
  • Journal Title: J. Photochem. Photobiol. A Volume: 331 Pages: 2-7
  • DOI: 10.1016/j.jphotochem.2016.08.006
  • Peer Reviewed
• [Journal Article] Highly Enantiodifferentiating Site of Human Serum Albumin for Mediating Photocyclodimerization of 2-Anthracenecarboxylate Elucidated by Site-Specific Inhibition/Quenching with Xenon (2016)
  • Author(s): Nishijima, M.; Pace, T. C. S.; Bohne, C.; Mori, T.; Inoue, Y.; Wada, T.
  • Journal Title: J. Photochem. Photobiol. A Volume: 331 Pages: 89-94
  • DOI: 10.1016/j.jphotochem.2015.12.019
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Control of Local Structures and Photophysical Properties of Zinc Porphyrin-Based Molecular Assemblies Structually Organized by Regioselctive Ligand Coordination (2016)
  • Author(s): T. Sakuma, H. Sakai, Y. Araki, T. Wada, T. Hasobe
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 18 Issue: 7 Pages: 5453-5463
  • DOI: 10.1039/c5cp07110k
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Synthetic Control of the Excited-State Dynamics and Circularly Polarized Luminescence of Fluorescent "push-Pull" Tetrathia[9]helicenes (2016)
  • Author(s): Yuki Yamamoto, Hayato Sakai, Junpei Yuasa, Yasuyuki Araki, Takehiko Wada, Tomo Sakanoue, Taishi Takenobu, Tsuyoshi Kawai, Taku Hasobe
  • Journal Title: Chemistry - A European Journal Volume: 22 Issue: 12 Pages: 4263-4273
  • DOI: 10.1002/chem.201504048
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Long-Lived Triplet Excited States of Bent-Shaped Pentacene Dimers by Intramolecular Singlet Fission (2016)
  • Author(s): T. Sakuma, H. Sakai, Y. Araki, T. Mori, T. Wada, N. V. Tkachenko, T. Hasobe
  • Journal Title: J. Phys. Chem. A Volume: 120 Issue: 11 Pages: 1867-1875
  • DOI: 10.1021/acs.jpca.6b00988
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Controlled Excited-State Dynamics and Enhanced Fluorescence Property of Tetrasulfone[9]helicene by a Simple Synthetic Process (2016)
  • Author(s): Yamamoto, Y; Sakai, H ; Yuasa, J ; Araki, Y; Wada, T ; Sakanoue, T ; Takenobu, T ; Kawai, T; Hasobe, T
  • Journal Title: JOURNAL OF PHYSICAL CHEMISTRY C Volume: 120 Issue: 13 Pages: 7421-7427
  • DOI: 10.1021/acs.jpcc.6b01123
  • Peer Reviewed
• [Journal Article] Acute development of collateral circulation and therapeutic prospects in ischemic stroke. (2016)
  • Author(s): Iwasawa E, Ichijo M, Ishibashi S, Yokota T.
  • Journal Title: Neural Regen Res. Volume: 11 Issue: 3 Pages: 368-71
  • DOI: 10.4103/1673-5374.179033
  • Peer Reviewed / Open Access
• [Journal Article] Module Strategy for Peptide Ribonucleic Acid (PRNA)-DNA and PRNA-Peptide Nucleic Acid (PNA)-DNA Chimeras: Synthesis and Interaction of Chimeras with DNA and RNA (2016)
  • Author(s): Uematsu, R.; Inagaki, M.; Asai, M.; Sugai, H.; Maeda, Y.; Nagami, A.; Sato, H.; Sakamoto, S.; Araki, Y.; Nishijima, M.; Inoue, Y.; Wada, T
  • Journal Title: Chemistry Letters Volume: 45 Issue: 3 Pages: 350-352
  • DOI: 10.1246/cl.151157
  • NAID: 130005132165
  • Peer Reviewed
• [Journal Article] Synthetic Control of Photophysical Process and Circularly Polarized Luminescence of [5]Carbohelicene Derivatives Substituted by Maleimide Units (2016)
  • Author(s): Hayato Sakai, Takako Kubota, Junpei Yuasa, Yasuyuki Araki, Tomo Sakanoue, Taishi Takenobu, Takehiko Wada, Tsuyoshi Kawai, and Taku Hasobe
  • Journal Title: Journal of Phyisical Chemistry C Volume: 120 Issue: 14 Pages: 7860-7869
  • DOI: 10.1021/acs.jpcc.6b01344
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Sphingosine-1-Phosphate Receptor-1 Selective Agonist Enhances Collateral Growth and Protects against Subsequent Stroke. (2015)
  • Author(s): Ichijo M, Ishibashi S, Li F, Yui D, Miki K, Mizusawa H, Yokota T.
  • Journal Title: PLoS One. Volume: 14 Issue: 10 Pages: 1839-45
  • DOI: 10.3174/ajnr.a4384
  • Peer Reviewed / Open Access
• [Journal Article] Hetero-Selective DNA-Like Duplex Stabilized by Donor-Acceptor Interactions (2015)
  • Author(s): Tetsuya Doi, Takumi Sakakibara, Hiromu Kashida, Yasuyuki Araki, Takehiko Wada, Hiroyuki Asanuma
  • Journal Title: Chem. Eur. J. Volume: 21 Issue: 45 Pages: 15974-15980
  • DOI: 10.1002/chem.201502653
  • Peer Reviewed / Open Access
• [Journal Article] Enhanced Phospholipase A2 Group 3 Expression by Oxidative Stress Decreases the Insulin-Degrading Enzyme (2015)
  • Author(s): Yui D, Nishida Y, Nishina T, Mogushi K, Tajiri M, Ishibashi S, Ajioka I, Ishikawa K, Mizusawa H, Murayama S, Yokota T
  • Journal Title: PLoS One. Volume: 10 Issue: 12 Pages: e0143518-e0143518
  • DOI: 10.1371/journal.pone.0143518
  • Peer Reviewed / Open Access
• [Journal Article] A Score for Predicting Paroxysmal Atrial Fibrillation in Acute Stroke Patients: iPAB Score (2015)
  • Author(s): Yoshioka K, Watanabe K, Zeniya S, Ito Y, Hizume M, Kanazawa T, Tomita M, Ishibashi S, Miake H, Tanaka H, Yokota T, Mizusawa H
  • Journal Title: J Stroke Cerebrovasc Dis Volume: 24 Issue: 10 Pages: 2263-9
  • DOI: 10.1016/j.jstrokecerebrovasdis.2015.06.019
  • Peer Reviewed
• [Journal Article] Chimeric Antisense Oligonucleotide Conjugated to α- Tocopherol (2015)
  • Author(s): Nishina T, Numata J, Nishina K, Yoshida-Tanaka K, Nitta K, Piao W, Iwata R, Ito S, Kuwahara H, Wada T, Mizusawa H, Yokota T
  • Journal Title: Mol Ther Nucleic Acids Volume: 4 Pages: e220-e220
  • DOI: 10.1038/mtna.2014.72
  • Peer Reviewed / Open Access
• [Journal Article] Synthesis and properties of vitamin E analog-conjugated neomycin for delivery of RNAi drugs to liver cells (2015)
  • Author(s): Iwata R, Nakayama F, Hirochi S, Sato K, Piao W, Nishina K, Yokota T, Wada T
  • Journal Title: Bioorg Med Chem. Lett Volume: 25 Issue: 4 Pages: 815-819
  • DOI: 10.1016/j.bmcl.2014.12.079
  • Peer Reviewed
• [Journal Article] Mammalian serum albumins as a chiral mediator library for bio-supramolecular photochirogenesis: optimizing enantiodifferentiating photocyclodimerization of 2-anthracenecarboxylate. (2014)
  • Author(s): Nishijima, M.; Goto, M.; Fujikawa, M.; Yang, C.; Mori, T.; Wada, T.; Inoue, Y.
  • Journal Title: Chem. Commun. Volume: 50 Issue: 91 Pages: 14082-14085
  • DOI: 10.1039/c4cc04818k
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Circular dichroism spectra of uridine derivatives: ChiraSac study (2014)
  • Author(s): 5) T. Miyahara, H. Nakatsuji, T. Wada
  • Journal Title: J. Phys. Chem. A Volume: 118 Issue: 16 Pages: 2931-2941
  • DOI: 10.1021/jp501906u
  • Peer Reviewed
• [Journal Article] Systematic control of the excited-state dynamics and carrier-transport properties of functionalized benzo[ghi]perylene and coronene derivatives (2014)
  • Author(s): S. Hirayama, H. Sakai, Y. Araki, M. Tanaka, M. Imakawa, T. Wada, T. Takenobu, T. Hasobe
  • Journal Title: Chem.- A Eur. J Volume: 20 Issue: 29 Pages: 9081-9093
  • DOI: 10.1002/chem.201304679
  • Peer Reviewed
• [Journal Article] Formation of One-Dimensional Helical Columns and Excimerlike Excited States by Racemic Quinoxaline-Fused [7] Carbohelicenes in the Crystal, (2014)
  • Author(s): H. Sakai, S. Shinto, Y. Araki, T. Wada, T. Sakanoue, T. Takenobu, T. Hasobe
  • Journal Title: Chem. - A Eur. J Volume: 20 Issue: 32 Pages: 10099-10109
  • DOI: 10.1002/chem.201402426
  • Peer Reviewed
• [Journal Article] Electron transfer dissociation mass spectrometry of peptides containing free cysteine using group XII metals as a charge carrier (2014)
  • Author(s): Asakawa D, Wada Y.
  • Journal Title: J Phys Chem B Volume: 118 Issue: 43 Pages: 12318-25
  • DOI: 10.1021/jp502818u
  • Peer Reviewed
• [Journal Article] Supramolecular Photocyclodimerization of 2-Hydroxyanthracene with Chiral Hydrogen-Bonding Template, Cyclodextrin and Serum Albumin (2014)
  • Author(s): G. Fukuhara, H. Umehara, S. Higashino, M. Nishijima, C. Yang, T. Mori, T. Wada, Y. Inoue
  • Journal Title: Photochemical & Photobiological Sciences Volume: 13 Issue: 2 Pages: 162-171
  • DOI: 10.1039/c3pp50127b
  • Peer Reviewed
• [Journal Article] Elevated platelet microparticle levels after acute ischemic stroke with concurrent idiopathic thrombocytopenic purpura (2014)
  • Author(s): Ichijo M, Ishibashi S, Ohkubo T, Nomura S, Sanjo N, Yokota T, Mizusawa H
  • Journal Title: J Stroke Cerebrovasc Dis Volume: 23 Issue: 3 Pages: 587-9
  • DOI: 10.1016/j.jstrokecerebrovasdis.2013.04.032
  • Peer Reviewed
• [Journal Article] Synthesis and properties of double-stranded RNA-bindable oligodiaminogalactose derivatives conjugated with vitamin E (2014)
  • Author(s): Rintaro Iwata, Kazutaka Nishina, Takanori Yokota, Takeshi Wada
  • Journal Title: Bioorg. Med. Chem. Volume: 22 Issue: 4 Pages: 1394-1403
  • DOI: 10.1016/j.bmc.2013.12.060
  • Peer Reviewed
• [Journal Article] Intrathecal AAV Serotype 9-mediated Delivery of shRNA Against TRPV1 Attenuates Thermal Hyperalgesia in a Mouse Model of Peripheral Nerve Injury. (2014)
  • Author(s): Hirai T, Enomoto M, Kaburagi H, Sotome S, Yoshida-Tanaka K, Ukegawa M, Kuwahara H, Yamamoto M, Tajiri M, Miyata H, Hirai Y, Tominaga M, Shinomiya K, Mizusawa H, Okawa A, Yokota T
  • Journal Title: Mol Ther Volume: 22 Issue: 2 Pages: 409-19
  • DOI: 10.1038/mt.2013.247
  • Peer Reviewed
• [Journal Article] Double Helices of a Pyridine-Appended Zinc Chlorophyll Derivative (2013)
  • Author(s): Y. Shinozaki, G. Richards, K. Ogawa, A. Yamano, K. Ohara, K. Yamaguchi, S. Kawano, K. Tanaka, Y. Araki, T. Wada, and J. Otsuki
  • Journal Title: J. Am. Chem. Soc. Volume: 135 Issue: 14 Pages: 5262-5265
  • DOI: 10.1021/ja400493e
  • Peer Reviewed
• [Presentation] SILENCING OF MALAT1 LONG NON-CODING RNA WITH DNA/RNA HETERODUPLEX OLIGONUCLEOTIDES EXACERBATES CEREBRAL ISCHEMIA (2017)
  • Author(s): Ishibashi S, Li L, Iwasawa E, Song J, Ichijo M, Zhang Y, Piao W, Yoshida K, Kuwahara H, Yoshioka K, Nagata T, Yokota T.
  • Organizer: XXIII World Congress of Neurology
  • Place of Presentation: KYOTO INTERNATIONAL CONFERENCE CENTER, Kyoto
  • Year and Date: 2017-09-16
  • Int'l Joint Research
• [Presentation] GENE SILENCING EFFECT OF HETERODUPLEX OLIGONUCLEOTIDE AND ITS BIODISTRIBUTION IN NEURONS AND BRAIN ENDOTHELIAL CELLS AFTER ISCHEMIA (2017)
  • Author(s): Li L, Ishibashi S, Iwasawa E, Song J, Ichijo M, Zhang Y, Piao W, Yoshida K, Kuwahara H, Yoshioka K, Nagata T, Yokota T
  • Organizer: XXIII World Congress of Neurology
  • Place of Presentation: KYOTO INTERNATIONAL CONFERENCE CENTER, Kyoto
  • Year and Date: 2017-09-16
  • Int'l Joint Research
• [Presentation] Heteroduplex oligonucleotide reduced gene expression in focal ischemic brain in mice (2017)
  • Author(s): Li L, Ishibashi S, Iwasawa E, Song J, Ichijo M, Zhang Y, Piao W, Yoshida K, Kuwahara H, Yoshioka K, Nagata T, Yokota T.
  • Organizer: STROKE2017
  • Place of Presentation: 大阪国際会議場, 大阪
• [Presentation] Expression of sphingosine-1-phosphate receptor 1 in leptomeningeal arteries is upregulated and its agonist improves neurological outcome in ischemic stroke in mice (2017)
  • Author(s): Iwasawa E, Li FY, Ishibashi S, Ichijo M, Miki K, Yokota T.
  • Organizer: XXIII World Congress of Neurology
  • Place of Presentation: KYOTO INTERNATIONAL CONFERENCE CENTER, Kyoto
  • Int'l Joint Research
• [Presentation] DEVELOPMENT of HIGH SENSITIVE AND HIGH TIME-RESOLVE CIRCULAR DICHROISM (CD) MEASUREMENT SYSTEM - Toward Analysis of Interaction Dynamics of Porphyrin Derivative with double stranded DNA - (2016)
  • Author(s): Wada T
  • Organizer: 4th Asia Chemical Biology Conference 2016
  • Place of Presentation: Kaohsiung, Taiwan
  • Year and Date: 2016-11-28
  • Invited
• [Presentation] Creation of Ischemia-Specifi c Oligonucleotide Therapeutics System with Intracellular Environmental Condition - Responsive Peptide Ribonucleic Acids (PRNAs) (2016)
  • Author(s): Wada T, Uematsu R, Asai M, Inagaki M, Sugai H, Araki Y, Sakamoto S, Ishibashi S, Yokota T, Wada T
  • Organizer: The 43nd International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Kumamoto Univ, Kumamoto
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] Creation of the ischemia-specific oligonucleotide therapeutics system with intracellular environment-responsive Peptide Ribonucleic Acids (PRNAs) : Development of half-gamer type chimeric PRNA-DNA-LNA derivatives (2016)
  • Author(s): Inagaki M, Uematsu R, Unabara D, Araki Y, Nakamoto S, Ishibashi S, Yokota T, Wada T
  • Organizer: The 43nd International Symposium on Nucleic Acids Chemistry
  • Place of Presentation: Kumamoto Univ, Kumamoto, Japan
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] Creation of hypoxia specific oligonucleotide therapeutics system with intracellular environment-responsible Peptide Ribonucleic Acids (PRNAs): ― Tuning of on - off switching pH of PRNA for optimization of hypoxia cell specificity (2016)
  • Author(s): Asai M, Uematsu R, Araki Y, Nakamoto S, Ishibashi S, Yokota T, Wada T
  • Organizer: Campus Asia Summer School Symposium 2016
  • Place of Presentation: Tohoku Univ, Sendai, Japan
  • Year and Date: 2016-08-27
  • Int'l Joint Research
• [Presentation] Creation of the hypoxia-specific oligonucleotide therapeutics system with intracellular environment-responsible Peptide Ribonucleic Acids (PRNAs): Synthesis and properties of new type of chimeric PRNA derivatives with 3’-DNA moiety introduced at N-terminus of PRNA (2016)
  • Author(s): Inagaki M, Uematsu R, Araki Y, Sakamoto S, Ishibashi S, Yokota T, Wada T
  • Organizer: Campus Asia Summer School Symposium 2016
  • Place of Presentation: Tohoku Univ, Sendai, Japan
  • Year and Date: 2016-08-27
  • Int'l Joint Research
• [Presentation] 「塞栓源不明の脳塞栓症」とはどのような病気か (2016)
  • Author(s): 石橋哲
  • Organizer: 第57回日本神経学会学術大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県神戸市）
  • Year and Date: 2016-05-18
  • Invited
• [Presentation] Upregulation and distribution of sphingosine-1-phosphate receptor 1 in acute ischemic stroke (2016)
  • Author(s): Iwasawa E, Ishibashi S, Ichijo M, Li F, Yokota T
  • Organizer: 第57回日本神経学会学術大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県神戸市）
  • Year and Date: 2016-05-18
• [Presentation] Heteroduplex Oligonucleotide reduced gene expression in focal ischemic brain in mice (2016)
  • Author(s): Li F, Ishibashi S, Yoshioka K, Wada T, Ichijo M, Iwasawa E, Song J, Zhang Y, Yokota T
  • Organizer: 第57回日本神経学会学術大会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県神戸市）
  • Year and Date: 2016-05-18
• [Presentation] SUPRAMOLECULAR ASYMMETRIC PHOTOCHIROHGEBESIS (SMAP) OF 2-ANTHRACENECARBOXYLATE (AC) WITH PROTEINS AND PROTEIN-POLYMER HYBRIDS AS CHIRAL REACTION MEDIAS - PEGYLATION OF HAS/BSA AND SMAP OF AC MEDIATED BY PEG-HAS/BSA- (2016)
  • Author(s): Wada T
  • Organizer: 2016 IUPAC Photochem
  • Place of Presentation: Osaka City Central Public Hall, Osaka
  • Year and Date: 2016-04-03
  • Invited
• [Presentation] Remarkable Enhancement of RNaseH Cleavage Activities of RNA Complexed with Peptide Ribonucleic Acid (PRNA) - DNA Chimera for Effective Cancer Cell Specific Oligonucleotide Therapeutics (2016)
  • Author(s): Wada T
  • Organizer: Institut Europeen de Chimie et Biologie Symposium
  • Place of Presentation: Bordeaux Univ., France
  • Invited
• [Presentation] Creation of hypoxia specific oligonucleotide therapeutics system with intracellular environment-responsible peptide ribonucleic acids (PRNAs): Tuning and optimization of on – off switching pH of PRNA for hypoxia specificity (2015)
  • Author(s): Asai M, Uematsu R, Araki Y, Nakamoto S, Ishibashi S, Yokota T, Wada T
  • Organizer: PACIFICHEM 2015
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Creation of the hypoxia-specific oligonucleotide therapeutics system with intracellular environment-responsible peptide ribonucleic acids (PRNAs): Synthesis and antisense activities of new type of chimeric PRNA-DNA derivatives containing phosphoramidate linkage in PRNADNA junction (2015)
  • Author(s): M. Inagaki, R. Uematsu, Y. Araki, S. SAKAMOTO, S. Ishibashi, H. Kashida, H. Asanuma, T. Yokota, T. Wada
  • Organizer: PACIFICHEM 2015
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Novel Strategy of Supramolecular Asymmetric Photochirogenesis with Tailor-made Biopolymers as Chiral Reaction Medias (2015)
  • Author(s): Wada T
  • Organizer: PACIFICHEM Molecular and Supramolecular Photochemistry 2015
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 2015-12-15
  • Int'l Joint Research / Invited
• [Presentation] 細胞内環境応答性ペプチドリボ核酸（PRNA）を利用したハイポキシア特異的核酸医薬の創製 (2015)
  • Author(s): 上松亮平, 浅井光夫, 稲垣雅仁, 荒木保幸, 坂本清志, 石橋哲, 横田隆徳, 和田健彦
  • Organizer: 第1回核酸医薬学会
  • Place of Presentation: 京都テルサ（京都府京都市）
  • Year and Date: 2015-11-30
• [Presentation] Remarkable Enhancement of RNaseH Cleavage and Antisense Activities of Peptide Ribonucleic Acid (PRNA) -DNA Chimera (2015)
  • Author(s): Wada T
  • Organizer: 11th Annual Meeting of the Oligonucleotide Therapeutics Society (OTS 2015)
  • Place of Presentation: Leiden, Netherlands
  • Year and Date: 2015-10-11
  • Int'l Joint Research
• [Presentation] 構造制御に基づく高機能核酸医薬用人工核酸の開 発 ― モデュール法によるペプチドリボ核酸（PRNA）の構造・ 機能制御への新戦略 (2015)
  • Author(s): 和田健彦, 上松亮平, 菅井祥加, 浅井光夫, 稲垣 雅仁, 荒木保幸, 坂本清志, 樫田啓, 浅沼浩之, 石橋哲, 横田隆徳
  • Organizer: 第64回高分子討論会
  • Place of Presentation: 東北大学（宮城県仙台市）
  • Year and Date: 2015-09-15
• [Presentation] Development of High Sensitive and Time-resolved Circular Dichroism Detection System–Toward the Analyses of Supramolecular Dynamic (2015)
  • Author(s): Wada T
  • Organizer: Circular Dichroism 2015
  • Place of Presentation: Hokkaido University, Sapporo, Japan
  • Year and Date: 2015-08-30
  • Invited
• [Presentation] Creation of hypoxia specific oligonucleotide therapeutics system with intracellular environment-responsible Peptide Ribonucleic Acids (PRNAs): ― Tuning of on - off switching pH of PRNA for optimization of hypoxia cell specificity (2015)
  • Author(s): Asai M, Uematsu R, Araki Y, Nakamoto S, Ishibashi S, Yokota T, Wada T
  • Organizer: Campus Asia Summer School Symposium 2015
  • Place of Presentation: Tohoku Univ, Sendai, Japan
  • Year and Date: 2015-08-27
  • Int'l Joint Research
• [Presentation] Development of High Sensitive and Time-resolved Circular Dichroism (CD) Detection System ‐Toward Analyses of Structural Change Dynamics of Biomolecules - The Interaction Dynamics of dsDNA-Cationic Porphyrin Comple (2015)
  • Author(s): Wada T
  • Organizer: Case Western Reserve University and Tohoku University Data and Life Science Collaboration & Symposium 2015
  • Place of Presentation: Cleveland, USA
  • Year and Date: 2015-07-29
  • Int'l Joint Research / Invited
• [Presentation] 生体界面と人工核酸の相互作用細胞膜透過性向上を指向したアルギニン残基導入とその効果 (2015)
  • Author(s): 菅井 祥加、上松 亮平、中瀬 生彦、坂本 清志、荒木 保幸、高井 まどか、石橋 哲、横田 隆徳、和田 健彦
  • Organizer: 生理研研究会「生体界面研究会」シンポジウム
  • Place of Presentation: 生理学研究所（愛知県岡崎市）
  • Year and Date: 2015-07-16
• [Presentation] Oligonucleotide reduced gene expression in focal ischemic brain in mice (2015)
  • Author(s): FuYing Li、石橋哲、一條真彦、和田健彦、横田隆徳
  • Organizer: 第56回に本神経学会学術集会
  • Place of Presentation: 新潟
  • Year and Date: 2015-05-21
  • Invited
• [Presentation] S1PR1 expression and endothelial cell proliferation after focal ischemia in mice (2015)
  • Author(s): 石橋哲、一條真彦、李富宝、三木 一徳、水澤 英洋、横田隆徳
  • Organizer: 第56回日本神経学会学術集会
  • Place of Presentation: 新潟
  • Year and Date: 2015-05-20
• [Presentation] 核酸医薬創薬の問題点と今後の方向性 (2015)
  • Author(s): 横田隆徳
  • Organizer: 日本化学会第95春季年会
  • Place of Presentation: 船橋
  • Year and Date: 2015-03-29
  • Invited
• [Presentation] DNA/RNA Heteroduplex Oligonucleotide as a New Class of Oligonucleotide (2015)
  • Author(s): Takanori Yokota
  • Organizer: 第7回Asia TIDES
  • Place of Presentation: 大阪
  • Year and Date: 2015-03-03
  • Invited
• [Presentation] 次世代核酸医薬の創出 (2014)
  • Author(s): 横田隆徳
  • Organizer: 第55回日本神経学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2014-05-21 – 2015-05-21
• [Book] 細胞内環境応答性ペプチドリボ核酸（PRNA）「核酸医薬の創製と応用展開」 (2015)
  • Author(s): 和田健彦
  • Publisher: CMC出版
• [Book] 光化学の事典、第２章 光化学の基礎I -物理化学-2.3節 励起状態の環境状態と反応 (2014)
  • Author(s): 和田健彦
  • Total Pages: 436
  • Publisher: 朝倉書房