Tomographic imaging of a cell by laser ultrasound microscope with femto-second laser

• Project/Area Number: 26282142
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Medical systems
• Research Institution: Tohoku University
• Principal Investigator: SAIJO Yoshifumi 東北大学, 医工学研究科, 教授 (00292277)
• Co-Investigator(Kenkyū-buntansha): 鷲尾 勝由 東北大学, 工学(系)研究科(研究院), 教授 (20417017)
• Co-Investigator(Renkei-kenkyūsha): MATSUURA Yuji 東北大学, 大学院医工学研究科, 教授 (10241530)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
• Keywords: 光超音波 / レーザー / 細胞 / バイオメカニクス / フェムト秒レーザー

Outline of Final Research Achievements

A laser ultrasound microscope with cellular resolution was developed by means of measuring high frequency laser ultrasound signal generated by ultra-short pulsed laser with ZnO thin film sensor. 3T3-L1 cells, which proliferated from fibroblast to adipocyte, were cultured on PET film and observed by the laser ultrasound microscope. C-mode and B-mode images were successfully obtained by the system. The method is capable of measuring sound speed and acoustic impedance and finally visualizes the distribution of Young’s elastic modulus and density within a cell. The method may contribute to the future investigation on cellular biomechanics.

Research Products

• [Journal Article] 3D Gabor wavelet based vessel filtering of photoacoustic images. (2016)
  • Author(s): Ul Haq I, Nagoaka R, Makino T, Tabata T, Saijo Y
  • Journal Title: Conf Proc 38th IEEE Eng Med Biol Soc Volume: - Pages: 3883-6
  • DOI: 10.1109/embc.2016.7591576
  • Peer Reviewed
• [Journal Article] Simultaneous evaluation of articular cartilage and subchondral bone from immobilized knee in rats by photoacoustic imaging system. (2015)
  • Author(s): Hagiwara Y, Izumi T, Yabe Y, Sato M, Sonofuchi K, Kanazawa K, Koide M, Saijo Y, Itoi E.
  • Journal Title: J Orthop Sci. Volume: 20 Issue: 2 Pages: 397-402
  • DOI: 10.1007/s00776-014-0692-2
  • Peer Reviewed / Open Access
• [Journal Article] Macrophage with gold nanorod visualized by optical-resolution and acoustic-resolution photoacoustic microscopes. (2015)
  • Author(s): Yamazaki R, Ogasawara K, Fujiwara M, Kobayashi K, Saijo Y
  • Journal Title: Conf Proc 37th IEEE Eng Med Biol Soc Volume: 1 Pages: 2387-2390
  • DOI: 10.1109/embc.2015.7318874
  • Peer Reviewed
• [Journal Article] Development of an ultrasound microscope combined with optical microscope for multiparametric characterization of a single cell. (2015)
  • Author(s): Arakawa M, Shikama J, Yoshida K, Nagaoka R, Kobayashi K, Saijo Y
  • Journal Title: IEEE Trans Ultrason Ferroelectr Freq Control Volume: 62 Issue: 9 Pages: 1615-1622
  • DOI: 10.1109/tuffc.2014.006865
  • Peer Reviewed
• [Presentation] Skin vasculature imaging by acoustical resolution photoacoustic microscope with parabolic array transducer. (2016)
  • Author(s): Saijo Y, Nagaoka R, Ul Haq I, Siregar S, Takagi R, Yoshizawa S, Umemura S
  • Organizer: 5th Joint Meeting of the Acoustical Society of America and the Acoustical Society of Japan
  • Place of Presentation: Honolulu, HI, USA.
  • Year and Date: 2016-11-28
  • Int'l Joint Research / Invited
• [Presentation] Photoacoustic imaging of vasculature with parabolic array transducer. (2016)
  • Author(s): Saijo Y, Nagaoka R, Takagi R, Yoshizawa S, Umemura S
  • Organizer: International Congress on Acoustics 2016
  • Place of Presentation: Buenos Aires, Argentine
  • Year and Date: 2016-09-05
  • Int'l Joint Research / Invited
• [Presentation] Recent developments of high frequency ultrasound imaging and its collaboration with optics. (2015)
  • Author(s): Saijo Y
  • Organizer: 12th Western Pacific Acoustics Conference 2015
  • Place of Presentation: Singapore (Singapore)
  • Year and Date: 2015-12-07
  • Int'l Joint Research / Invited
• [Presentation] Adaptive spatial filtering with principal component analysis for biomedical photoacoustic imaging. (2015)
  • Author(s): Nagaoka R, Yamazaki R, Saijo Y
  • Organizer: International Congress on Ultrasonics 2015
  • Place of Presentation: Metz (France)
  • Year and Date: 2015-05-11
  • Int'l Joint Research
• [Presentation] Photoacoustic imaging of macrophage with gold nanorod. (2015)
  • Author(s): Saijo Y, Yamazaki R, Ogasawara K
  • Organizer: The 5th Asian and Pacific-Rim Symposium on Biophotonics APBP ’15
  • Place of Presentation: パシフィコ横浜（神奈川県・横浜市）
  • Year and Date: 2015-04-23
  • Int'l Joint Research
• [Presentation] Photoacoustic micro imaging of gold nanorod phagocytosing macrophage (2015)
  • Author(s): Saijo Y
  • Organizer: Optics in Cardiology 2015
  • Place of Presentation: Rotterdam, The Netherlands
  • Year and Date: 2015-03-11 – 2015-03-13
• [Presentation] Characterization of a single cell with multiple ultrasound parameters (2014)
  • Author(s): Saijo Y, Shikama J, Yoshida K, Nagaoka R, Arakawa M, Kobayashi K, O’Brien Jr W
  • Organizer: 9th International Conference on Ultrasonic Biomedical Microscanning
  • Place of Presentation: Edinburgh, United Kingdom
  • Year and Date: 2014-09-28 – 2014-10-01
• [Remarks] 西條研究室ホームページ
  • URL: http://www.ecei.tohoku.ac.jp/imaging/index.htm
• [Remarks] 西條研究室
  • URL: http://www.ecei.tohoku.ac.jp/imaging/index.htm