| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Outline of Final Research Achievements |
In order to produce structural diversity of compounds, combinatorial biosyntheses of the polyketide cyclase, olivetolic acid cyclase (OAC) and the type III polyketide synthase, octaketide synthase from Aloe arborescens using structurally distinct various CoA thioesters as the substrates were carried out. As a result, we found that the co-incubation of the enzymes using hexanoyl-CoA and malonyl-CoA as the substrates led to a production of novel naphthalene. Furthermore, we solved OAC apo and OAC-olivetolic acid binary complex structures at 1.4 angstrom and 1.7 angstrom resolutions, respectively. The crystallographic and site-directed mutagenesis studies of OAC revealed that the enzyme possesses the pentyl-binding pocket that plays an important role to bind the pentyl moiety of the substrate and employs Tyr72 and His78 as the catalytic residues.
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